scholarly journals Residual sodium dodecyl sulfate in decellularized muscle matrices leads to fibroblast activation in vitro and foreign body response in vivo

2017 ◽  
Vol 12 (3) ◽  
Author(s):  
Emily E. Friedrich ◽  
Steven T. Lanier ◽  
Solmaz Niknam‐Bienia ◽  
Gabriel A. Arenas ◽  
Divya Rajendran ◽  
...  
Keyword(s):  
Foreign Body ◽  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
Foreign Body Response ◽  
Fibroblast Activation ◽  
Dodecyl Sulfate ◽  
Body Response

scholarly journals Biodegradation of poly(anhydride-esters) into non-steroidal anti-inflammatory drugs and their effect on Pseudomonas aeruginosa biofilms in vitro and on the foreign-body response in vivo

Biomaterials ◽  
2006 ◽  
Vol 27 (29) ◽  
pp. 5039-5048 ◽  
Author(s):  
James D. Bryers ◽  
Rebecca A. Jarvis ◽  
Jason Lebo ◽  
Almudena Prudencio ◽  
Themis R. Kyriakides ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Foreign Body ◽  
Foreign Body Response ◽  
Inflammatory Drugs ◽  
Anti Inflammatory ◽  
Body Response

scholarly journals In vitro and in vivo evaluation of sodium dodecyl sulfate (SDS) as an inactivator of caprine lentivirus (CLV) in colostrum and milk

2018 ◽  
Vol 70 (5) ◽  
pp. 1459-1467 ◽  
Author(s):  
A.L.M. Sousa ◽  
R.R. Pinheiro ◽  
J.F. Araújo ◽  
V.W.S. Santos ◽  
D.A.A. Azevedo ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Inhibitory Activity ◽  
Sodium Dodecyl ◽  
In Vivo Evaluation ◽  
Nested Polymerase Chain Reaction ◽  
Vitro Experiment ◽  
In Vitro Experiment ◽  
Dodecyl Sulfate

ABSTRACT The aim of this study was to evaluate in vitro and in vivo the effect of sodium dodecyl sulfate (SDS) on the caprine lentivirus (CLV) in colostrum and milk. This was performed to develop a practical and efficient method of blocking the lactogenic transmission of the virus. In the in vitro experiment, colostrum and milk were treated with 0.25%; 0.50% and 1% SDS. Then, somatic cells of colostrum and milk were submitted to co-culture with caprine synovial membrane cells (CSM). In the in vivo test, goats were fed with colostrum and milk provided from CLV-positive goats treated with SDS in the same concentrations used in the in vitro experiment. Animals were tested by nested polymerase chain reaction (nPCR) and Western blot (WB) assays. In the in vitro experiment, inhibitory activity against CLV without inactivation occurred in colostrum with all SDS concentrations. However, concentrations of 0.25 and 0.5% SDS presented only inhibitory activity against CLV in milk cells, and 1% concentration provided inactivation of the virus. In the in vivo tests, none of the three concentrations of SDS was effective in inactivating LVC in colostrum or goat milk, which was confirmed by seroconversion and presence of proviral DNA in animals afterwards.

scholarly journals Assessing the Effects of VEGF Releasing Microspheres on the Angiogenic and Foreign Body Response to a 3D Printed Silicone-Based Macroencapsulation Device

Pharmaceutics ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2077
Author(s):  
Ruth E. Levey ◽  
Fergal B. Coulter ◽  
Karina C. Scheiner ◽  
Stefano Deotti ◽  
Scott T. Robinson ◽  
...  
Keyword(s):  
Foreign Body ◽  
Blood Vessels ◽  
Foreign Body Response ◽  
Islet Cell Transplantation ◽  
Clinical Implementation ◽  
Multi Scale ◽  
Significant Difference ◽  
Body Response

Macroencapsulation systems have been developed to improve islet cell transplantation but can induce a foreign body response (FBR). The development of neovascularization adjacent to the device is vital for the survival of encapsulated islets and is a limitation for long-term device success. Previously we developed additive manufactured multi-scale porosity implants, which demonstrated a 2.5-fold increase in tissue vascularity and integration surrounding the implant when compared to a non-textured implant. In parallel to this, we have developed poly(ε-caprolactone-PEG-ε-caprolactone)-b-poly(L-lactide) multiblock copolymer microspheres containing VEGF, which exhibited continued release of bioactive VEGF for 4-weeks in vitro. In the present study, we describe the next step towards clinical implementation of an islet macroencapsulation device by combining a multi-scale porosity device with VEGF releasing microspheres in a rodent model to assess prevascularization over a 4-week period. An in vivo estimation of vascular volume showed a significant increase in vascularity (* p = 0.0132) surrounding the +VEGF vs. −VEGF devices, however, histological assessment of blood vessels per area revealed no significant difference. Further histological analysis revealed significant increases in blood vessel stability and maturity (** p = 0.0040) and vessel diameter size (*** p = 0.0002) surrounding the +VEGF devices. We also demonstrate that the addition of VEGF microspheres did not cause a heightened FBR. In conclusion, we demonstrate that the combination of VEGF microspheres with our multi-scale porous macroencapsulation device, can encourage the formation of significantly larger, stable, and mature blood vessels without exacerbating the FBR.

scholarly journals Sodium Dodecyl Sulfate and C31G as Microbicidal Alternatives to Nonoxynol 9: Comparative Sensitivity of Primary Human Vaginal Keratinocytes

2000 ◽  
Vol 44 (7) ◽  
pp. 1954-1960 ◽  
Author(s):  
Fred C. Krebs ◽  
Shendra R. Miller ◽  
Bradley J. Catalone ◽  
Patricia A. Welsh ◽  
Daniel Malamud ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Cell Viability ◽  
Sodium Dodecyl ◽  
Transmitted Disease ◽  
Cell Types ◽  
Direct Immunofluorescence ◽  
Sexually Transmitted ◽  
Dodecyl Sulfate

ABSTRACT A broad-spectrum vaginal microbicide must be effective against a variety of sexually transmitted disease pathogens and be minimally toxic to the cell types found within the vaginal epithelium, including vaginal keratinocytes. We assessed the sensitivity of primary human vaginal keratinocytes to potential topical vaginal microbicides nonoxynol-9 (N-9), C31G, and sodium dodecyl sulfate (SDS). Direct immunofluorescence and fluorescence-activated cell sorting analyses demonstrated that primary vaginal keratinocytes expressed epithelial cell-specific keratin proteins. Experiments that compared vaginal keratinocyte sensitivity to each agent during a continuous, 48-h exposure demonstrated that primary vaginal keratinocytes were almost five times more sensitive to N-9 than to either C31G or SDS. To evaluate the effect of multiple microbicide exposures on cell viability, primary vaginal keratinocytes were exposed to N-9, C31G, or SDS three times during a 78-h period. In these experiments, cells were considerably more sensitive to C31G than to N-9 or SDS at lower concentrations within the range tested. When agent concentrations were chosen to result in an endpoint of 25% viability after three daily exposures, each exposure decreased cell viability at the same constant rate. When time-dependent sensitivity during a continuous 48-h exposure was examined, exposure to C31G for 18 h resulted in losses in cell viability not caused by either N-9 or SDS until at least 24 to 48 h. Cumulatively, these results reveal important variations in time- and concentration-dependent sensitivity to N-9, C31G, or SDS within populations of primary human vaginal keratinocytes cultured in vitro. These investigations represent initial steps toward both in vitro modeling of the vaginal microenvironment and studies of factors that impact the in vivo efficacy of vaginal topical microbicides.

scholarly journals Chitosan/Sodium Dodecyl Sulfate Complexes for Microencapsulation of Vitamin E and Its Release Profile—Understanding the Effect of Anionic Surfactant

2021 ◽  
Vol 15 (1) ◽  
pp. 54
Author(s):  
Jelena Milinković Budinčić ◽  
Lidija Petrović ◽  
Ljiljana Đekić ◽  
Milijana Aleksić ◽  
Jadranka Fraj ◽  
...  
Keyword(s):  
Vitamin E ◽  
Sodium Dodecyl Sulfate ◽  
Anionic Surfactant ◽  
Sodium Dodecyl ◽  
Pharmaceutical Products ◽  
Release Mechanism ◽  
Bioactive Substances ◽  
Water Emulsion ◽  
Dodecyl Sulfate

Microencapsulation of bioactive substances is a common strategy for their protection and release rate control. The use of chitosan (Ch) is particularly promising due to its abundance, biocompatibility, and interaction with anionic surfactants to form complexes of different characteristics with relevance for use in microcapsule wall design. In this study, Ch/sodium dodecyl sulfate (SDS) microcapsules, without and with cross-linking agent (formaldehyde (FA) or glutaraldehyde (GA)), were obtained by the spray drying of vitamin E loaded oil-in-water emulsion. All of the microcapsules had good stability during the drying process. Depending on the composition, their product yield, moisture content, and encapsulation efficiency varied between 11–34%, 1.14–1.62%, and 94–126%, respectively. SEM and FTIR analysis results indicate that SDS as well as cross-linkers significantly affected the microcapsule wall properties. The profiles of in vitro vitamin E release from the investigated microcapsules fit with the Korsmeyer-Peppas model (r2 > 0.9). The chemical structure of the anionic surfactant was found to have a significant effect on the vitamin E release mechanism. Ch/SDS coacervates may build a microcapsule wall without toxic crosslinkers. This enabled the combined diffusion/swelling based release mechanism of the encapsulated lipophilic substance, which can be considered favorable for utilization in food and pharmaceutical products.

scholarly journals Comparison of In Vitro and Cell-Mediated Alteration of a Human Rhinovirus and Its Inhibition by Sodium Dodecyl Sulfate 1

1973 ◽  
Vol 12 (4) ◽  
pp. 819-826 ◽  
Author(s):  
K. Lonberg-Holm ◽  
J. Noble-Harvey
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
Human Rhinovirus ◽  
Dodecyl Sulfate

scholarly journals Comparative In Vitro Sensitivities of Human Immune Cell Lines, Vaginal and Cervical Epithelial Cell Lines, and Primary Cells to Candidate Microbicides Nonoxynol 9, C31G, and Sodium Dodecyl Sulfate

2002 ◽  
Vol 46 (7) ◽  
pp. 2292-2298 ◽  
Author(s):  
Fred C. Krebs ◽  
Shendra R. Miller ◽  
Bradley J. Catalone ◽  
Raina Fichorova ◽  
Deborah Anderson ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Sodium Dodecyl Sulfate ◽  
Cell Viability ◽  
Cell Lines ◽  
Sodium Dodecyl ◽  
Primary Cells ◽  
Cytokine Activation ◽  
Dodecyl Sulfate ◽  
Human Immune

ABSTRACT In experiments to assess the in vitro impact of the candidate microbicides nonoxynol 9 (N-9), C31G, and sodium dodecyl sulfate (SDS) on human immune and epithelial cell viability, cell lines and primary cell populations of lymphocytic and monocytic origin were generally shown to be equally sensitive to exposures ranging from 10 min to 48 h. However, U-937 cells were more sensitive to N-9 and C31G after 48 h than were primary monocyte-derived macrophages. Cytokine activation of monocytes and lymphocytes had no effect on cell viability following exposure to these microbicidal compounds. Primary and passaged vaginal epithelial cultures and cell lines differed in sensitivity to N-9 and C31G but not SDS. These studies provide a foundation for in vitro experiments in which cell lines of human immune and epithelial origin can be used as suitable surrogates for primary cells to further investigate the effects of microbicides on cell metabolism, membrane composition, and integrity and the effects of cell type, proliferation, and differentiation on microbicide sensitivity.

Decellularization of porcine carotid arteries using low-concentration sodium dodecyl sulfate

2020 ◽  
pp. 039139882097542
Author(s):  
Jin Cheng ◽  
Ji Li ◽  
Zhiwen Cai ◽  
Yuehao Xing ◽  
Cong Wang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Sodium Dodecyl Sulfate ◽  
Carotid Arteries ◽  
Sodium Dodecyl ◽  
Dimensional Structure ◽  
Low Concentration ◽  
Dodecyl Sulfate ◽  
Decellularized Scaffolds ◽  
Triton X

Background: The decellularized scaffold is a promising material for producing tissue-engineered vascular grafts (TEVGs) because of its complex, native-like three-dimensional structure and mechanical properties. Sodium dodecyl sulfate (SDS), one of the most commonly used decellularization reagents, appears to be more effective than other detergents for removing cells from dense tissues. The concentrations of SDS used in previous studies and their effects on decellularization are not consistent. Methods: In this study, porcine carotid arteries were decellularized using detergent-based protocols using Triton X-100 followed by SDS at different concentrations and exposing time. Cell removal efficiency and composition were evaluated by histological analysis, and DNA and collagen quantification. Ultrastructure, mechanical properties, pore size distribution, and in vivo biocompatibility of decellularized arteries were also evaluated. Results: The DNA content of decellularized scaffolds treated with 0.3% SDS for 72 h or 0.5% SDS for 48 h was significantly less than that treated with 1% SDS for 30 h. There was a significant loss of soluble collagen after treatment with 1% SDS relative to native arteries. The extensive loss of elastin and glycosaminoglycans was observed in decellularized arteries treated with 0.5% SDS or 1% SDS. The basement membrane and biomechanics were also damaged by these two protocols. Moreover, decellularized scaffolds became more porous with many large pores after treatment with 0.3% SDS. Conclusion: Low-concentration SDS could be a suitable choice for artery decellularization. Decellularized porcine carotid arteries, prepared using Triton X-100 followed by 0.3% SDS, may be a promising biological scaffold for TEVGs.

In vivo modulation of foreign body response on polyurethane by surface entrapment technique

2010 ◽  
Vol 95A (2) ◽  
pp. 413-423 ◽  
Author(s):  
Anand P. Khandwekar ◽  
Deepak P. Patil ◽  
Anand A. Hardikar ◽  
Yogesh S. Shouche ◽  
Mukesh Doble
Keyword(s):  
Foreign Body ◽  
Foreign Body Response ◽  
Body Response
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