Ascorbic Acid Content and Transcriptional Profiling of Genes Involved in Its Metabolism during Development of Petals, Leaves, and Fruits of Orange (Citrus sinensis cv. Valencia Late)

Citrus fruit is one of the most important contributors to the ascorbic acid (AsA) intake in humans. Here, we report a comparative analysis of AsA content and transcriptional changes of genes related to its metabolism during development of petals, leaves and fruits of Valencia Late oranges (Citrus sinensis). Petals of close flowers and at anthesis contained the highest concentration of AsA. In fruits, AsA content in the flavedo reached a maximum at color break, whereas the pulp accumulated lower levels and experienced minor fluctuations during development. AsA levels in leaves were similar to those in the flavedo at breaker stage. The transcriptional profiling of AsA biosynthetic, degradation, and recycling genes revealed a complex and specific interplay of the different pathways for each tissue. The D-galacturonic acid pathway appeared to be relevant in petals, whereas in leaves the L-galactose pathway (GGP and GME) also contributed to AsA accumulation. In the flavedo, AsA content was positively correlated with the expression of GGP of the L-galactose pathway and negatively with DHAR1 gene of the recycling pathway. In the pulp, AsA appeared to be mainly controlled by the coordination among the D-galacturonic acid pathway and the MIOX and GalDH genes. Analysis of the promoters of AsA metabolism genes revealed a number of cis-acting elements related to developmental signals, but their functionalities remain to be investigated.

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Analysis of ascorbic acid content in various fruits and vegetables by spectrofluorimetric methods

Planta Medica ◽

10.1055/s-0031-1282624 ◽

2011 ◽

Vol 77 (12) ◽

Cited By ~ 2

Author(s):

A Haskovic ◽

A Copra Janicijevic ◽

A Topcagic ◽

L Klepo ◽

A Kapur ◽

...

Keyword(s):

Ascorbic Acid ◽

Acid Content ◽

Fruits And Vegetables ◽

Ascorbic Acid Content

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EFFECTS OF PSEUDOHYPOPHYSECTOMY (UNDERFEEDING), STARVATION, HORMONES AND AGING UPON THE ASCORBIC ACID CONTENT OF THE ADRJENAL GLANDS AND LIVER OF THE RAT

Endocrinology ◽

10.1210/endo-31-2-276 ◽

1942 ◽

Vol 31 (2) ◽

pp. 276-281 ◽

Cited By ~ 12

Author(s):

MICHAEL G. MULINOS ◽

LEO POMERANTZ ◽

MARY E. LOJKIN

Keyword(s):

Ascorbic Acid ◽

Acid Content ◽

Ascorbic Acid Content

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Silicon attenuates calcium deficiency by increasing ascorbic acid content, growth and quality of cabbage leaves

Scientific Reports ◽

10.1038/s41598-020-80934-6 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Dalila Lopes da Silva ◽

Renato de Mello Prado ◽

Luis Felipe Lata Tenesaca ◽

José Lucas Farias da Silva ◽

Ben-Hur Mattiuz

Keyword(s):

Ascorbic Acid ◽

Nutrient Solution ◽

Acid Content ◽

Dry Matter ◽

Ascorbic Acid Content ◽

Calcium Deficiency ◽

Ca Deficiency ◽

Leaf Water Loss ◽

Nutrient Solutions

AbstractCalcium (Ca) deficiency in cabbage plants induces oxidative damage, hampering growth and decreasing quality, however, it is hypothesized that silicon (Si) added to the nutrient solution may alleviate crop losses. Therefore, this study aims at evaluating whether silicon supplied in the nutrient solution reduces, in fact, the calcium deficiency effects on cabbage plants. In a greenhouse, cabbage plants were grown using nutrient solutions with Ca sufficiency and Ca deficiency (5 mM) without and with added silicon (2.5 mM), arranged as a 2 × 2 factorial in randomized blocks, with five replications. At 91 days after transplanting, the plants were harvested for biological evaluations. In the treatment without added Si, Ca deficiency promoted oxidative stress, low antioxidant content, decreased dry matter, and lower quality leaf. On the other hand, added Si attenuated Ca deficiency in cabbage by decreasing cell extravasation while increasing both ascorbic acid content and fresh and dry matter, providing firmer leaves due to diminished leaf water loss after harvesting. We highlighted the agronomic importance of Si added to the nutrient solution, especially in crops at risk of Ca deficiency.

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Optimising Tropical Fruit Juice Quality Using Thermosonication-Assisted Extraction via Blocked Face-Centered Composite Design

Processes ◽

10.3390/pr9010003 ◽

2020 ◽

Vol 9 (1) ◽

pp. 3

Author(s):

Norazlin Abdullah ◽

Nyuk Ling Chin

Keyword(s):

Ascorbic Acid ◽

Fruit Juice ◽

Ascorbic Acid Content ◽

Soluble Solids ◽

Quadratic Model ◽

Juice Quality ◽

Tropical Fruit ◽

Total Soluble Solids ◽

Juice Yield ◽

Solids Content

Extraction of tropical fruit juice using simple, efficient, and environmentally friendly technologies is gaining importance to produce high quality juices. Juice from pink-fleshed guava, pink-fleshed pomelo, and soursop was extracted using direct and indirect thermosonication methods by varying intensity, time, and temperature, and compared to those extracted using water bath incubation. Improvised models of juice yield, ascorbic acid, and total soluble solids responses were generated by eliminating insignificant model terms of the factors in full quadratic model using backward eliminating procedure. Main effects, 3D, or 4D plots for each response were developed based on factors that influenced the response. Results showed that the best extraction method for guava and pomelo juices were within indirect thermosonication method of 1 kW, 55 °C and 30 min, and 2.5 kW, 54 °C and 23 min, respectively. Direct thermosonication method at 10% amplitude, 55 °C for 2 to 10 min was more suitable for soursop juice. Thermosonicated extraction of tropical fruit juice can improve its juice yield, ascorbic acid content, and total soluble solids content.

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COMPARISON OF ASCORBIC ACID CONTENT IN IMPORTED TOMATOES AND IN CULTIVARS GROWN IN NOVA SCOTIA

Canadian Journal of Plant Science ◽

10.4141/cjps87-047 ◽

1987 ◽

Vol 67 (1) ◽

pp. 331-335

Author(s):

HAK-YOON JU ◽

W. JOHN MULLIN

Keyword(s):

Ascorbic Acid ◽

Nova Scotia ◽

Vitamin C ◽

Acid Content ◽

Ascorbic Acid Content ◽

Fresh Weight ◽

Atlantic Region ◽

Significant Difference ◽

Cluster Location ◽

Greenhouse Tomatoes

The ascorbic acid (vitamin C) content of fresh imported field tomatoes and Nova Scotia greenhouse and field tomatoes was determined on a bi-weekly basis during the period of availability of each type of tomato to the Nova Scotia consumer in 1984. The average ascorbic acid contents of imported and Nova Scotia field and greenhouse tomatoes were 13.3, 16.7 and 17.7 mg 100 g−1 fresh weight, respectively. A study of nine recommended or promising field tomatoes for the Atlantic region showed significant differences in ascorbic acid content among the cultivars. The cultivar Quick Pick had the highest ascorbic acid content of 22.5 ± 1.5 mg 100 g−1, the cultivar Campbell 18 had the lowest content, 12.0 ± 2.9 mg 100 g−1. In Dombito greenhouse tomatoes the stage of maturity and the effect of cluster location were tested against ascorbic acid content. The lowest ascorbic acid content of 9.1 ± 1.0 mg 100 g−1 was found with the small green tomatoes while others from mature green to overripe contained 14.0–16.7 mg 100 g−1. Tomatoes from different cluster locations showed no significant difference in ascorbic acid content.Key words: Vitamin C, L-ascorbic acid, tomatoes

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Ascorbic Acid Content, pH, and Acceptability of Tomatoes Processed by Different Home Canning Methods

Home Economics Research Journal ◽

10.1177/1077727x7800600406 ◽

1978 ◽

Vol 6 (4) ◽

pp. 305-311 ◽

Cited By ~ 3

Author(s):

Marilyn M. Skelton ◽

Charles W. Marr

Keyword(s):

Ascorbic Acid ◽

Acid Content ◽

Ascorbic Acid Content

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Antioxidant Potential in Different Parts and Callus ofGynura procumbensand Different Parts ofGynura bicolor

BioMed Research International ◽

10.1155/2015/147909 ◽

2015 ◽

Vol 2015 ◽

pp. 1-7 ◽

Cited By ~ 15

Author(s):

Vijendren Krishnan ◽

Syahida Ahmad ◽

Maziah Mahmood

Keyword(s):

Ascorbic Acid ◽

Acid Content ◽

Phenolic Content ◽

Reducing Power ◽

Ascorbic Acid Content ◽

Total Phenolic ◽

Total Flavonoid Content ◽

Dpph Radical ◽

Flavonoid Content ◽

Different Parts

Plants from Gynura family was used in this study, namely,Gynura procumbensandGynura bicolor.Gynura procumbensis well known for its various medicinal properties such as antihyperglycaemic, antihyperlipidaemic, and antiulcerogenic; meanwhile,G. bicolorremains unexploited. Several nonenzymatic antioxidants methods were utilized to study the antioxidant capacity, which include ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, total flavonoid content, total phenolic content, and ascorbic acid content determination. DPPH assay revealsG. procumbensshoot as the lowest (66.885%) andG. procumbensroot as the highest (93.499%) DPPH radical inhibitor. In FRAP assay, reducing power was not detected inG. procumbensleaf callus (0.000 TEAC mg/g FW) wherebyG. procumbensroot exhibits the highest (1.103 TEAC mg/g FW) ferric reducing power. Total phenolic content and total flavonoid content exhibited similar trend for both the intact plants analysed. In all antioxidant assays,G. procumbenscallus culture exhibits very low antioxidant activity. However,G. procumbensroot exhibited highest phenolic content, flavonoid content, and ascorbic acid content with 4.957 TEAC mg/g FW, 543.529 QEµg/g FW, and 54.723 µg/g FW, respectively. This study reveals thatG. procumbensroot extract is a good source of natural antioxidant.

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THE EFFECT OF LOW TEMPERATURE ON THE ASCORBIC ACID CONTENT OF THE OVOTESTIS OF THE SLUG, ARION SUBFUSCUS (DRAPARNAUD)

Canadian Journal of Zoology ◽

10.1139/z57-053 ◽

1957 ◽

Vol 35 (5) ◽

pp. 609-613

Author(s):

D. Pelluet

Keyword(s):

Ascorbic Acid ◽

Low Temperature ◽

Acid Content ◽

Ascorbic Acid Content ◽

Arion Subfuscus ◽

Initial Decrease

The amount of ascorbic acid in the slug Arion subfuscus has been estimated quantitatively at laboratory temperatures and at 0°–2 °C. The results show that the effect of the exposure to the low temperature reduces the amount-present in the ovotestis significantly. In general, the amount of ascorbic acid in the cold treated animals does not exceed that of the controls. This result does not agree with the cytological appearance of the ovotestis exposed to the same conditions, in which the cold treated animals show an increased number of granules of ascorbic acid after an initial decrease.

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