scholarly journals Genetics of Clubroot and Fusarium Wilt Disease Resistance in Brassica Vegetables: The Application of Marker Assisted Breeding for Disease Resistance

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 726 ◽  
Author(s):  
Hasan Mehraj ◽  
Ayasha Akter ◽  
Naomi Miyaji ◽  
Junji Miyazaki ◽  
Daniel J. Shea ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Fusarium Wilt ◽  
Interleukin 1 ◽  
Wilt Disease ◽  
R Genes ◽  
Vegetable Crops ◽  
Marker Assisted Breeding ◽  
Resistant Genes ◽  
Brassica Vegetables ◽  
Lrr Protein

The genus Brassica contains important vegetable crops, which serve as a source of oil seed, condiments, and forages. However, their production is hampered by various diseases such as clubroot and Fusarium wilt, especially in Brassica vegetables. Soil-borne diseases are difficult to manage by traditional methods. Host resistance is an important tool for minimizing disease and many types of resistance (R) genes have been identified. More than 20 major clubroot (CR) disease-related loci have been identified in Brassica vegetables and several CR-resistant genes have been isolated by map-based cloning. Fusarium wilt resistant genes in Brassica vegetables have also been isolated. These isolated R genes encode the toll-interleukin-1 receptor/nucleotide-binding site/leucine-rice-repeat (TIR-NBS-LRR) protein. DNA markers that are linked with disease resistance allele have been successfully applied to improve disease resistance through marker-assisted selection (MAS). In this review, we focused on the recent status of identifying clubroot and Fusarium wilt R genes and the feasibility of using MAS for developing disease resistance cultivars in Brassica vegetables.

Cloning of disease-resistance homologues in end sequences of BAC clones linked to Fom-2, a gene conferring resistance to Fusarium wilt in melon (Cucumis melo L.)

Genome ◽  
2002 ◽  
Vol 45 (3) ◽  
pp. 473-480 ◽  
Author(s):  
Yi-Hong Wang ◽  
Woobong Choi ◽  
Claude E Thomas ◽  
Ralph A Dean
Keyword(s):  
Disease Resistance ◽  
Fusarium Wilt ◽  
Cucumis Melo ◽  
Interleukin 1 ◽  
Bac Library ◽  
Artificial Chromosome ◽  
Sequence Information ◽  
R Genes ◽  
Nucleotide Binding Sites ◽  
Cucumis Melo L

Disease resistance has not yet been characterized at the molecular level in cucurbits, a group of high-value, nutritious, horticultural plants. Previously, we genetically mapped the Fom-2 gene that confers resistance to Fusarium wilt races 0 and 1 of melon. In this paper, two cosegregating codominant markers (AM, AFLP marker; FM, Fusarium marker) were used to screen a melon bacterial artificial chromosome (BAC) library. Identified clones were fingerprinted and end sequenced. Fingerprinting analysis showed that clones identified by each marker assembled into two separate contigs at high stringency. GenBank searches produced matches to leucine-rich repeats (LRRs) of resistance genes (R genes); to retroelements and to cellulose synthase in clones identified by FM; and to nucleotide-binding sites (NBSs) of R genes, retroelements, and cytochrome P-450 in clones identified by AM. A 6.5-kb fragment containing both NBS and LRR sequences was found to share high homology to TIR (Toll-interleukin-1 receptor)–NBS–LRR R genes, such as N, with 42% identity and 58% similarity in the TIR–NBS and LRR regions. The sequence information may be useful for identifying NBS–LRR class of R genes in other cucurbits.Key words: BAC end sequencing, Cucumis melo L., Fusarium wilt, R gene.

Selection for Fusarium wilt disease resistance from regenerants derived from leaf callus of strawberry

1991 ◽  
Vol 10 (4) ◽  
Author(s):  
Hideyoshi Toyoda ◽  
Koji Horikoshi ◽  
Yasuyoshi Yamano ◽  
Seiji Ouchi
Keyword(s):  
Disease Resistance ◽  
Fusarium Wilt ◽  
Wilt Disease ◽  
Fusarium Wilt Disease ◽  
Selection For

scholarly journals Breeding for Disease Resistance in Brassica Vegetables Using DNA Marker Selection

2021 ◽  
Author(s):  
Mst Arjina Akter ◽  
Hasan Mehraj ◽  
Takeru Itabashi ◽  
Tomoe Shindo ◽  
Masaaki Osaka ◽  
...  
Keyword(s):  
Fusarium Wilt ◽  
Crop Production ◽  
Asian Country ◽  
Plant Diseases ◽  
R Genes ◽  
Global Food Security ◽  
Resistant Cultivars ◽  
Marker Selection ◽  
Disease Resistant ◽  
Brassica Vegetables

The Brassica genus comprises of agro-economically important vegetables. Disease causes great yield loss of Brassica vegetables worldwide. Different traditional methods such as crop rotation and chemical control have limited effect on different diseases of Brassica vegetables and cannot completely eradicate the pathogens by these methods. Development of disease resistant cultivars is one of the most effective, ecofriendly, and cheapest measure to control Brassica diseases. With the development of genomics, molecular biology techniques, and biological methods, it is possible to discover and introduce resistance (R) genes to efficiently control the plant diseases caused by pathogens. Some R genes of major diseases such as Fusarium wilt and clubroot in Brassica vegetables have been already identified. Therefore, we will focus to review the Fusarium wilt and clubroot resistance in Brassica vegetables and the methodologies for identification, mapping, and pyramiding of R genes/quantitative trait loci (QTLs) to develop disease resistant cultivars. These techniques will be helpful for sustainable crop production and to maintain global food security and contribute to ensure protection of food supply in the Asian country as well as throughout the world.

scholarly journals Development of a Mitochondrial SCAR Marker Related to Susceptibility of Banana (Musa AAA Cavendish) to Fusarium oxysporum f. sp. Cubense Race 4

2018 ◽  
Vol 46 (2) ◽  
pp. 509-516
Author(s):  
Fang WANG ◽  
Ling XIA ◽  
Shun LV ◽  
Chunxiang XU ◽  
Yuqing NIU ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Mitochondrial Genome ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Scar Marker ◽  
Wilt Disease ◽  
Scar Markers ◽  
Resistant Cultivars ◽  
Fusarium Wilt Disease ◽  
Race 4

The use of resistant cultivars is an effective method for the control of banana (Musa spp.) Fusarium wilt caused by race 4 of Fusarium oxysporum f. sp. cubense (Foc4). However, selection of disease-resistant cultivars requires large-scale field evaluations and is time-consuming. Development of early, reliable, and reproducible selection strategies can speed up this process. Sequence characterized amplified region (SCAR) markers have been widely employed in the resistant breeding of many crops. However, to date, there have been no reports about the presence of plant disease resistance-related SCAR markers in mitochondrial genome yet, which also plays a very important role in plant defenses. In the present study, a sequence-related amplified polymorphism (SRAP) marker, a specific fragment of 829 bp, was identified. This fragment could be amplified from Foc4-susceptible but not from the resistant cultivars. It was located in banana mitochondrial genome and mapped near the putative cytochrome c biogenesis ccmB-like mitochondrial protein. This fragment was then successfully converted into a SCAR marker, namely Mito-Foc-S001, which was found to be able to discriminate the resistance from susceptibility to Fusarium wilt disease of bananas with the discriminatory power of the new mark being 96.88%. Thus, this marker can be used in banana (Musa AAA Cavendish) breeding for Fusarium wilt disease resistance.

scholarly journals Genome-Wide Characterization of NBS-Encoding Genes in Watermelon and Their Potential Association with Gummy Stem Blight Resistance

2019 ◽  
Vol 20 (4) ◽  
pp. 902 ◽  
Author(s):  
Md Hassan ◽  
Md Rahim ◽  
Hee-Jeong Jung ◽  
Jong-In Park ◽  
Hoy-Taek Kim ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Citrullus Lanatus ◽  
Interleukin 1 ◽  
Expression Patterns ◽  
Defense Responses ◽  
Economic Losses ◽  
R Genes ◽  
Stem Blight ◽  
Gummy Stem Blight ◽  
Encoding Genes

Watermelon (Citrullus lanatus) is a nutritionally rich and economically important horticultural crop of the Cucurbitaceae family. Gummy stem blight (GSB) is a major disease of watermelon, which is caused by the fungus Didymella bryoniae, and results in substantial economic losses in terms of yield and quality. However, only a few molecular studies have focused on GSB resistance in watermelon. Nucleotide binding site (NBS)-encoding resistance (R) genes play important roles in plant defense responses to several pathogens, but little is known about the role of NBS-encoding genes in disease resistance in watermelon. The analyzed NBS-encoding R genes comprises several domains, including Toll/interleukin-1 receptor(TIR), NBS, leucine-rich repeat (LRR), resistance to powdery mildew8(RPW8) and coiled coil (CC), which are known to be involved in disease resistance. We determined the expression patterns of these R genes in resistant and susceptible watermelon lines at different time points after D. bryoniae infection by quantitative RT-PCR. The R genes exhibited various expression patterns in the resistant watermelon compared to the susceptible watermelon. Only six R genes exhibited consistent expression patterns (Cla001821, Cla019863, Cla020705, Cla012430, Cla012433 and Cla012439), which were higher in the resistant line compared to the susceptible line. Our study provides fundamental insights into the NBS-LRR gene family in watermelon in response to D. bryoniae infection. Further functional studies of these six candidate resistance genes should help to advance breeding programs aimed at improving disease resistance in watermelons.

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