scholarly journals Evaluation of the Antiwrinkle Activity of Enriched Isatidis Folium Extract and an HPLC–UV Method for the Quality Control of Its Cream Products

Plants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1586
Author(s):  
Dan Gao ◽  
Chong Woon Cho ◽  
Cheong Taek Kim ◽  
Won Seok Jeong ◽  
Jong Seong Kang
Keyword(s):  
Quality Control ◽  
Matrix Metalloproteinase ◽  
Mrna Expression ◽  
Manufacturing Process ◽  
High Performance ◽  
Chemical Constituents ◽  
Hplc Method ◽  
Matrix Metalloproteinase 3 ◽  
Matrix Metalloproteinase 1 ◽  
Entire Manufacturing Process

Currently, many extracts from natural sources are added to cosmetic products for reducing facial aging and wrinkles. This study investigated the antiwrinkle activity of enriched extract of Isatidis Folium used for a novel antiwrinkle cream product. The result demonstrated that this enriched extract has excellent antiwrinkle activity by significantly inhibiting mRNA expression of matrix metalloproteinase-1, matrix metalloproteinase-3, and pro-inflammatory cytokines IL-1β and upregulating the mRNA expression of IL-4 and procollagen. Additionally, to implement effective quality control of the entire manufacturing process of antiwrinkle cream products based on the enriched extract of Isatidis Folium, the main chemical constituents of the enriched extract of Isatidis Folium was evaluated by high–performance liquid chromatography-photodiode array-tandem mass spectrometry (HPLC-PDA-ESI-MS/MS), five constituents were undisputedly confirmed. An HPLC-UV method in 15-min analysis time for quality assessment of the entire manufacturing process of antiwrinkle cream products was proposed and validated. The optimal conditions for extracting TMCA (3,4,5-trimethoxycinnamic acid) from the developed antiwrinkle cream products were determined using response surface methodology based on central composite design. The established HPLC method and optimal extract condition are suitable for routinely analyzing this novel antiwrinkle cream product.

scholarly journals Quantitative determination of ciprofloxacin and levofloxacin antibacterials by Spectrophotometeric and high performance liquid chromatography

2015 ◽  
Vol 11 (1) ◽  
Author(s):  
Muhammad Akram ◽  
Jamil Anwar ◽  
Ammar Z. Alshemarya ◽  
Yi-Fan Goh ◽  
Ahmed Sher Awan ◽  
...  
Keyword(s):  
Quality Control ◽  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Drug Effect ◽  
Hplc Method ◽  
Linear Calibration ◽  
Pharmaceutical Tablets ◽  
Pure Compounds ◽  
The Stability

A simple, rapid and sensitive Spectrophotometeric method for the determination of fluoroquinolones; ciprofloxacin and levofloxacin have been performed in pure form and pharmaceutical tablets. Both drugs gave reddish complexes when treated with iron (III) chloride at pH 4.0. The drugs showed maximum absorption at 530 and 545 nm. In both cases linear calibration was obtained up to 0.9 mg/10 mL of the drug. Effect of different parameters like pH, temperature and time was also studied on the stability of the complexes. The percentage recoveries found by described method was in the range of 98.2---100.01 %. Standards were prepared from the pure compounds obtained from sigma-Aldrich Pharm. The method was successfully employed for the Assay of drugs in commercial formulations. Finally determination of the drugs was carried out through HPLC method which showed that there is no appreciable difference between the results of both the methods. Results revealed that proposed method is practically suitable for routine applications in quality control laboratories for the analysis of fluoroquinolones drugs.________________________________________GRAPHICAL ABSTRACT

scholarly journals Matrix metalloproteinase-1 and matrix metalloproteinase-3 gene promoter polymorphisms are associated with mortality in haemodialysis patients

2009 ◽  
Vol 24 (7) ◽  
pp. 2207-2212 ◽  
Author(s):  
Mario Cozzolino ◽  
Maria Luisa Biondi ◽  
Andrea Galassi ◽  
Olivia Turri ◽  
Diego Brancaccio ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Gene Promoter ◽  
Promoter Polymorphisms ◽  
Matrix Metalloproteinase 3 ◽  
Matrix Metalloproteinase 1

scholarly journals Analytical procedure for quality control of 18F-Choline radiopharmaceutical

2021 ◽  
Vol 63 (5) ◽  
pp. 10-16
Author(s):  
Thanh Quang Vu ◽  
◽  
Ngoc Khoan Ha ◽  
Thanh Rin Bui ◽  
Trung Dung Nguyen ◽  
...  
Keyword(s):  
Quality Control ◽  
High Performance ◽  
Radiochemical Purity ◽  
Analytical Procedure ◽  
Hplc Method ◽  
Central Hospital ◽  
Residual Solvents ◽  
Quality Specifications ◽  
Pet Radiopharmaceutical ◽  
Required Quality

18Fluoromethylcholine (18F-Choline, 18F-FCH) has been produced using a home-made automatic synthesiser at 30 MeV Cyclotron Centre, 108 Military Central Hospital. In order to be licensed using for patients, the 18F-FCH radiopharmaceutical needs to meet the required quality specifications listed in the pharmacopeia. The objective of this study was to build up the analytical procedure and to perform for the 18F-FCH quality control followed the EuPh2017 pharmacopeia for the PET radiopharmaceutical. The result obtained confirmed that the total time to complete the test of one sample of the 18F-FCH was less than 35 min. Identifications of 18F-FCH, radiochemical purity, and 18F content were determined with accuracy >96% by High-performance liquid chromatography (HPLC) method and content of residual solvents consists of Ethanol, Acetonitrile, Dibromomethane, Dimethylethanolamine were determined with the accuracy >97% by Gas chromatography (GC) method.

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