scholarly journals Mass Spectrometric Characterization of Epoxidized Vegetable Oils

Polymers ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 394
Author(s):  
Ákos Kuki ◽  
Tibor Nagy ◽  
Mahir Hashimov ◽  
Stella File ◽  
Miklós Nagy ◽  
...  
Keyword(s):  
Raw Materials ◽  
Ionization Mass ◽  
Mass Spectral Peak ◽  
Mass Spectral ◽  
Natural Systems ◽  
Data Mining Approach ◽  
Novel Approach ◽  
Fragmentation Mechanisms ◽  
Fatty Acid Compositions

Matrix-assisted laser desorption ionization and electrospray ionization mass spectrometry (MALDI-MS and ESI-MS) were used for the characterization of epoxidized soybean and linseed oils, which are important raw materials in the biopolymer production. The recently invented data mining approach, mass-remainder analysis (MARA), was implemented for the analysis of these types of complex natural systems. Different epoxidized triglyceride mass spectral peak series were identified, and the number of carbon atoms and epoxide groups was determined. The fragmentation mechanisms of the epoxidized triglyceride (ETG) adducts formed with different cations (such as H+, Na+, Li+, and NH4+) were explored. As a novel approach, the evaluation of the clear fragmentation pathways of the sodiated ETG adducts enabled the estimation of the epoxidized fatty acid compositions of these types of oils by MS/MS.

Electrospray Ionization Mass Spectral Characterization of Transient Iron Species of Bioinorganic Relevance

1996 ◽  
Vol 35 (8) ◽  
pp. 2369-2372 ◽  
Author(s):  
Jinheung Kim ◽  
Yanhong Dong ◽  
Edmund Larka ◽  
Lawrence Que
Keyword(s):  
Electrospray Ionization ◽  
Spectral Characterization ◽  
Ionization Mass ◽  
Iron Species ◽  
Mass Spectral

scholarly journals Standardized measure for phosphatidylinositol, 4,5-bisphosphate by mass spectrometry

2021 ◽  
Author(s):  
Jaspreet Jawanda
Keyword(s):  
Mass Spectrometry ◽  
Membrane Trafficking ◽  
High Performance ◽  
Fold Increase ◽  
Mass Spectral ◽  
Physiological Processes ◽  
Novel Approach ◽  
Nuclear Events ◽  
Standardized Measure

Phosphoinositides (PIs) play a fundamental role in many physiological processes such as cell surface signal transduction, membrane trafficking, cytoskeleton regulation, nuclear events and permeability and transport functions of membranes. Levels of PIs vary under different physiological conditions thus PI profiling may be an important step in elucidation of importance in the progression towards many diseases. Previous methods for PI analysis have several disadvantages in time-constraints, use of radioactive samples and inconsistent results due to lack of sensitivity. Mass spectrometry has previously been utilized to quantify low abundance peptides, metabolites and lipids. Here we propose a novel approach for PI quantitation based on inositol head cleavage coupled to high-performance liquid-mass spectrometry (HPLC-MS) to overcome these issues. First, we highlight the extraction and deacylation of PIs from S. Ceravisiae, followed by purification via reverse phase chromatography with a Sep-Pak. Next, using commercially purchased PI (4,5) P2 standards, we created a tune file which provide the correct conditions sensitive enough to identify prolific ion peaks to be utilized in characterization of biological samples. Using the standard tune file, we successfully identified and quantitated the PI (4,5) P2 in cells lacking INP51 which have 2-4 fold increase in PI (4,5)P(subscript 2) and comparing them to the wild type cells. The methods described may form a basis for further optimization of mass spectral based quantitation of PIs.

scholarly journals Enhanced Morphological Characterization of Cellulose Nano/Microfibers through Image Skeleton Analysis

Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2077
Author(s):  
Jose Luis Sanchez-Salvador ◽  
Cristina Campano ◽  
Patricio Lopez-Exposito ◽  
Quim Tarrés ◽  
Pere Mutjé ◽  
...  
Keyword(s):  
Raw Materials ◽  
Morphological Characterization ◽  
Gel Point ◽  
Mechanical Pretreatment ◽  
Novel Approach ◽  
Point Data ◽  
And Control ◽  
Microscopy Images ◽  
Mediated Oxidation

The present paper proposes a novel approach for the morphological characterization of cellulose nano and microfibers suspensions (CMF/CNFs) based on the analysis of eroded CMF/CNF microscopy images. This approach offers a detailed morphological characterization and quantification of the micro and nanofibers networks present in the product, which allows the mode of fibrillation associated to the different CMF/CNF extraction conditions to be discerned. This information is needed to control CMF/CNF quality during industrial production. Five cellulose raw materials, from wood and non-wood sources, were subjected to mechanical, enzymatic, and (2,2,6,6-Tetramethylpiperidin-1-yl)oxyl (TEMPO)-mediated oxidative pre-treatments followed by different homogenization sequences to obtain products of different morphologies. Skeleton analysis of microscopy images provided in-depth morphological information of CMF/CNFs that, complemented with aspect ratio information, estimated from gel point data, allowed the quantification of: (i) fibers peeling after mechanical pretreatment; (ii) fibers shortening induced by enzymes, and (iii) CMF/CNF entanglement from TEMPO-mediated oxidation. Being mostly based on optical microscopy and image analysis, the present method is easy to implement at industrial scale as a tool to monitor and control CMF/CNF quality and homogeneity.

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