Immobilization of Platelet-Rich Plasma onto COOH Plasma-Coated PCL Nanofibers Boost Viability and Proliferation of Human Mesenchymal Stem Cells

AbstractSkeletal muscle tissue is characterized by restrained self-regenerative capabilities, being ineffective in relation to trauma extension both in time span (e.g. chronic diseases) and in size (e.g. large trauma). For these reasons, tissue engineering and/or cellular therapies represent a valuable solution in the cases where the physiological healing process failed. Satellite cells, the putative skeletal muscle stem cells, have been the first solution explored to remedy the insufficient self-regeneration capacity. Nevertheless, some limitation related to donor age, muscle condition, expansion hitch and myogenic potentiality maintenance have limited their use as therapeutic tool. To overcome this hindrance, different stem cells population with myogenic capabilities have been investigated to evaluate their real potentiality for therapeutic approaches, but, as of today, the perfect cell candidate has not been identified yet.In this work, we analyze the characteristics of skeletal muscle-derived human Mesenchymal Stem Cells (hMSCs), showing the maintenance/increment of myogenic activity upon differential culture conditions. In particular, we investigate the influence of a commercial enriched growth medium (Cyto-Grow), and of a medium enriched with either human-derived serum (H.S.) or Platelet-rich Plasma (PrP), in order to set up a culture protocol useful for employing this cell population in clinical therapeutic strategies. The presented results reveal the remarkable effects of H.S. in the enhancement of hMSC proliferation and myogenic differentiation.

Download Full-text

Human Mesenchymal Stem Cells Behavior on Synthetic Coral Scaffold

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.696.205 ◽

2016 ◽

Vol 696 ◽

pp. 205-211 ◽

Cited By ~ 5

Author(s):

Erlina Sih Mahanani ◽

Indra Bachtiar ◽

Ika Dewi Ana

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Cell Attachment ◽

Platelet Rich Plasma ◽

Human Mesenchymal Stem Cells ◽

Control Group ◽

Freeze Dried ◽

Initial Support ◽

The One ◽

Coral Scaffold

For bone tissue engineering, corals have long history to be used as scaffold to promote bone regeneration. However the use of a lot of corals may damage their habitates. For this reason, a strategy to mimic coral in a synthetic form is needed. As an ideal scaffold, synthetic coral must provide structure and initial support for cell attachment and proliferation. The aim of this study was to investigate the attachment and proliferation of human Mesenchymal Stem Cells (h-MSC) on synthetic coral scaffold, to provide information on the behavior of h-MSC on the designated scaffold. Synthetic coral scaffolds were prepared from bovine gelatine and CaCO3 with 5:5 in 10% w/v concentration in aquadest. Sodium citrate was used as dispersant in the suspension. Gelatin-CaCO3 suspension was moulded in a plastic cover of 24 well plate, then freezed at -20°C for 24 hours, freeze dried for 24 hours and continued by dehydrothermal crosslinking for 72 hours. After the fabrication, synthetic coral scaffolds were subjects to cover the bottom of the well for cell culture. Human Mesenchymal Stem Cells (h-MSC) were seeded and divided into 2 groups, control group without scaffold and the one with scaffold. All groups were incubated for 3, 6, and 24 hours. Cells attatchment were determined by deduction of the cells unattached from total cells seeding. Proliferation of h-MSC were done in 3 groups ie., control group without scaffold, scaffold only and scaffold incorporated Platelet Rich Plasma (PRP) in the bottom of well. All groups were incubated for 24, 48 and 72 hours. Human Mesenchymal Stem Cells attached faster to synthetic coral scaffold than the control. Its proliferation behavior was faster in the scaffold incorporated PRP, showing better interaction of scaffold and cells with the incorporation of morphogenetic factor.

Download Full-text