scholarly journals Antifungal Effect of Volatile Organic Compounds from Bacillus velezensis CT32 against Verticillium dahliae and Fusarium oxysporum

Processes ◽  
2020 ◽  
Vol 8 (12) ◽  
pp. 1674
Author(s):  
Xinxin Li ◽  
Xiuhong Wang ◽  
Xiangyuan Shi ◽  
Baoping Wang ◽  
Meiping Li ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Volatile Organic Compounds ◽  
Fusarium Oxysporum ◽  
Organic Compounds ◽  
Verticillium Dahliae ◽  
Inhibitory Effect ◽  
Rrna Gene ◽  
Vascular Wilt ◽  
Bacillus Velezensis ◽  
Volatile Organic

The present study focuses on the inhibitory effect of volatile metabolites released by Bacillus velezensis CT32 on Verticillium dahliae and Fusarium oxysporum, the causal agents of strawberry vascular wilt. The CT32 strain was isolated from maize straw compost tea and identified as B. velezensis based on 16S rRNA gene sequence analysis. Bioassays conducted in sealed plates revealed that the volatile organic compounds (VOCs) produced by the strain CT32 possessed broad-spectrum antifungal activity against eight phytopathogenic fungi. The volatile profile of strain CT32 was obtained by headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). A total of 30 volatile compounds were identified, six of which have not previously been detected in bacteria or fungi: (Z)-5-undecene, decyl formate, 2,4-dimethyl-6-tert-butylphenol, dodecanenitrile, 2-methylpentadecane and 2,2’,5,5’-tetramethyl-1,1’-biphenyl. Pure compounds were tested in vitro for their inhibitory effect on the mycelial growth of V. dahliae and F. oxysporum. Decanal, benzothiazole, 3-undecanone, 2-undecanone, 2-undecanol, undecanal and 2,4-dimethyl-6-tert-butylphenol showed high antifungal activity, with benzothiazole and 2,4-dimethyl-6-tert-butylphenol being the most potent compounds. These results indicate that the VOCs produced by B. velezensis CT32 have the potential to be used as a biofumigant for management of vascular wilt pathogens.

scholarly journals Different Fecal Microbiotas and Volatile Organic Compounds in Treated and Untreated Children with Celiac Disease

2009 ◽  
Vol 75 (12) ◽  
pp. 3963-3971 ◽  
Author(s):  
Raffaella Di Cagno ◽  
Carlo G. Rizzello ◽  
Francesca Gagliardi ◽  
Patrizia Ricciuti ◽  
Maurice Ndagijimana ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Solid Phase ◽  
Lactobacillus Delbrueckii ◽  
Rrna Gene ◽  
Healthy Children ◽  
Fecal Samples ◽  
Dgge Analysis ◽  
Volatile Organic

ABSTRACTThis study aimed at investigating the fecal microbiotas of children with celiac disease (CD) before (U-CD) and after (T-CD) they were fed a gluten-free diet and of healthy children (HC). Brothers or sisters of T-CD were enrolled as HC. Each group consisted of seven children. PCR-denaturing gradient gel electrophoresis (DGGE) analysis with V3 universal primers revealed a unique profile for each fecal sample. PCR-DGGE analysis with group- or genus-specific 16S rRNA gene primers showed that theLactobacilluscommunity of U-CD changed significantly, while the diversity of theLactobacilluscommunity of T-CD was quite comparable to that of HC. Compared to HC, the ratio of cultivable lactic acid bacteria andBifidobacteriumtoBacteroidesand enterobacteria was lower in T-CD and even lower in U-CD. The percentages of strains identified as lactobacilli differed as follows: HC (ca. 38%) > T-CD (ca. 17%) > U-CD (ca. 10%).Lactobacillus brevis,Lactobacillus rossiae, andLactobacillus pentosuswere identified only in fecal samples from T-CD and HC.Lactobacillus fermentum,Lactobacillus delbrueckiisubsp.bulgaricus, andLactobacillus gasseriwere identified only in several fecal samples from HC. Compared to HC, the composition ofBifidobacteriumspecies of T-CD varied, and it varied even more for U-CD. Forty-seven volatile organic compounds (VOCs) belonging to different chemical classes were identified using gas-chromatography mass spectrometry-solid-phase microextraction analysis. The median concentrations varied markedly for HC, T-CD, and U-CD. Overall, ther2values for VOC data for brothers and sisters were equal to or lower than those for unrelated HC and T-CD. This study shows the effect of CD pathology on the fecal microbiotas of children.

scholarly journals Antifungal properties of volatile organic compounds produced by Daldinia eschscholtzii MFLUCC 19-0493 isolated from Barleria prionitis leaves against Colletotrichum acutatum and its post-harvest infections on strawberry fruits

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11242
Author(s):  
Sarunpron Khruengsai ◽  
Patcharee Pripdeevech ◽  
Chutima Tanapichatsakul ◽  
Chanin Srisuwannapa ◽  
Priya Esilda D’Souza ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Endophytic Fungi ◽  
Colletotrichum Acutatum ◽  
Anthracnose Disease ◽  
Post Harvest ◽  
Volatile Organic

Fungal endophytes are microorganisms living symbiotically with a host plant. They can produce volatile organic compounds (VOCs) that have antimicrobial activity. This study aimed to isolate endophytic fungi from Barleria prionitis plants grown in Thailand and to investigate the antifungal properties of their VOCs against Colletotrichum acutatum, a causal agent of anthracnose disease on post-harvest strawberry fruits. A total of 34 endophytic fungi were isolated from leaves of B. prionitis. The VOCs produced from each individual isolate were screened for their antifungal activity against C. acutatum using a dual-culture plate method. From this in vitro screening experiment, the VOCs produced by the endophytic isolate BP11 were found to have the highest inhibition percentage (80.3%) against the mycelial growth of C. acutatum. The endophytic isolate BP11 was molecularly identified as Daldinia eschscholtzii MFLUCC 19-0493. This strain was then selected for an in vivo experiment. Results from the in vivo experiment indicated that the VOCs produced by D. eschscholtzii MFLUCC 19-0493 were able to inhibit infections by C. acutatum on organic fresh strawberry fruits with an average inhibition percentage of 72.4%. The quality of the pathogen-inoculated strawberry fruits treated with VOCs produced by D. eschscholtzii MFLUCC 19-0493 was evaluated. Their fruit firmness, total soluble solids, and pH were found to be similar to the untreated strawberry fruits. Solid phase microextraction-gas chromatographic-mass spectrometric analysis of the VOCs produced by D. eschscholtzii MFLUCC 19-0493 led to the detection and identification of 60 compounds. The major compounds were elemicin (23.8%), benzaldehyde dimethyl acetal (8.5%), ethyl sorbate (6.8%), methyl geranate (6.5%), trans-sabinene hydrate (5.4%), and 3,5-dimethyl-4-heptanone (5.1%). Each major compound was tested for its antifungal activity against C. acutatum using the in vitro assay. While all these selected VOCs showed varying degrees of antifungal activity, elemicin was found to possess the strongest antifungal activity. This work suggests that D. eschscholtzii MFLUCC 19-0493 could be a promising natural preservative for controlling C. acutatum associated anthracnose disease in strawberry fruits during the post-harvest period.

scholarly journals Characterization of Volatile Organic Compounds Emitted from Endophytic Burkholderia cenocepacia ETR-B22 by SPME-GC-MS and Their Inhibitory Activity against Various Plant Fungal Pathogens

Molecules ◽  
2020 ◽  
Vol 25 (17) ◽  
pp. 3765
Author(s):  
Jian-Hua Chen ◽  
Wei Xiang ◽  
Ke-Xin Cao ◽  
Xuan Lu ◽  
Shao-Chang Yao ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Broad Spectrum ◽  
Fungal Pathogens ◽  
Control Plant ◽  
Burkholderia Cenocepacia ◽  
Nonanoic Acid ◽  
Antagonistic Microorganisms ◽  
Volatile Organic

The use of antagonistic microorganisms and their volatile organic compounds (VOCs) to control plant fungal pathogens is an eco-friendly and promising substitute for chemical fungicides. In this work, endophytic bacterium ETR-B22, isolated from the root of Sophora tonkinensis Gagnep., was found to exhibit strong antagonistic activity against 12 fungal pathogens found in agriculture. Strain ETR-B22 was identified as Burkholderia cenocepacia based on 16S rRNA and recA sequences. We evaluated the antifungal activity of VOCs emitted by ETR-B22. The VOCs from strain ETR-B22 also showed broad-spectrum antifungal activity against 12 fungal pathogens. The composition of the volatile profiles was analyzed based on headspace solid phase microextraction (HS-SPME) gas chromatography coupled to mass spectrometry (GC-MS). Different extraction strategies for the SPME process significantly affected the extraction efficiency of the VOCs. Thirty-two different VOCs were identified. Among the VOC of ETR-B22, dimethyl trisulfide, indole, methyl anthranilate, methyl salicylate, methyl benzoate, benzyl propionate, benzyl acetate, 3,5-di-tert-butylphenol, allyl benzyl ether and nonanoic acid showed broad-spectrum antifungal activity, and are key inhibitory compounds produced by strain ETR-B22 against various fungal pathogens. Our results suggest that the endophytic strain ETR-B22 and its VOCs have high potential for use as biological controls of plant fungal pathogens.

scholarly journals Identification and analysis of odor-active compounds from Choerospondias axillaris (Roxb.) Burtt et Hill with different moisture content levels and lacquer treatments

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Qifan Wang ◽  
Jun Shen ◽  
Bin Zeng ◽  
Huiyu Wang
Keyword(s):  
Volatile Organic Compounds ◽  
Moisture Content ◽  
Organic Compounds ◽  
Inhibitory Effect ◽  
Waterborne Coating ◽  
Active Compounds ◽  
Antiseptic Solution ◽  
Volatile Organic ◽  
Gas Chromatography Mass Spectroscopy ◽  
Total Volatile Organic Compounds

Abstract The problem of indoor odors can greatly affect a room’s occupants. To identify odorants and comprehensively evaluate emissions from wooden materials, emissions and odors from Choerospondias axillaris (Roxb.) Burtt et Hill with different moisture content percentages and lacquer treatments were investigated in this study. Thermal desorption–gas chromatography–mass spectroscopy/olfactometry was used to analyze the release characteristics. In total, 11 key odor-active compounds were identified as moisture content gradually decreased, concentrating between 15 and 33 min. Total volatile organic compounds, total very volatile organic compounds, and total odor intensity decreased as moisture content decreased. In addition, 35 odor-active compounds, including aromatics, alkenes, aldehydes, esters, and alcohols, were identified in the odor control list. Polyurethane (PU), ultraviolet (UV), and waterborne coatings had a good inhibitory effect on eight odor characteristics, but some scents arose after lacquer treatment. For equilibrium moisture content, the major characteristics of Choerospondias axillaris were fragrant (9.4) and mint-like (3.0) compared with the fragrant (8.2), fruity (7.8), and pleasant (5.8) characteristics of PU coating; the flowery (5.9), fragrant (5.0), glue-like (4.3), and pineapple-like (4.3) characteristics of UV coating; and the antiseptic solution (3.6), fragrant (2.9), cigarette-like (2.8), and fruity (2.5) characteristics of waterborne coating. Based on multicomponent evaluation, a Choerospondias axillaris board with waterborne coating was suggested for use indoors.

scholarly journals Antifungal activity of volatile organic compounds fromStreptomyces alboflavusTD-1

2013 ◽  
Vol 341 (1) ◽  
pp. 45-51 ◽  
Author(s):  
Changlu Wang ◽  
Zhifang Wang ◽  
Xi Qiao ◽  
Zhenjing Li ◽  
Fengjuan Li ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Volatile Organic
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