Capability of Immobilized Clostridium beijerinckii TISTR 1461 on Lotus Stalk Pieces to Produce Butanol from Sugarcane Molasses

Immobilized Clostridium beijerinckii TISTR 1461 was used to enhance the butanol production efficiency from sugarcane molasses. Lotus stalk (LS) pieces were used as carriers for cell immobilization. Sugarcane molasses containing 50 g/L of sugar supplemented with 1 g/L of yeast extract was found to be an appropriate medium for bacterial cell immobilization on the LS pieces. Carrier size (4, 12 and 20 mm in length) and carrier loading (1:15, 1:30 and 1:45, w/v) were optimized for high levels of butanol production using response surface methodology (RSM). The batch fermentation was carried out under anaerobic conditions in 1 L screw-capped bottles at 37 °C and an agitation rate of 150 rpm. It was found that the optimum conditions for the butanol production were the carrier size of 4 mm and carrier loading of 1:31 (w/v). Under these conditions, the butanol concentration (PB) was 12.89 g/L, corresponding to the butanol productivity (QB) of 0.36 g/L∙h and butanol yield (YB/S) of 0.36 g/g. These values were higher than those using free cells (PB, 10.20 g/L, QB, 0.28 g/L∙h and YB/S, 0.32 g/g). In addition, it was found that a 24 h incubation time for cell immobilization was appropriate for the immobilization process, which was confirmed by the results of the scanning electron microscope (SEM) and atomic force microscopy (AFM) images and specific surface area measurement. When the fermentation using the immobilized cells was carried out in a stirred-tank reactor (STR), column reactor (CR) and CR coupled with STR, the results showed that all reactors could be used to produce butanol production from the immobilized cells on LS pieces. However, the PB using CR and CR coupled with STR were only 75% and 45% of those using the screw-capped bottle and STR.

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Impacts of Initial Sugar, Nitrogen and Calcium Carbonate on Butanol Fermentation from Sugarcane Molasses by Clostridium beijerinckii

Energies ◽

10.3390/en13030694 ◽

2020 ◽

Vol 13 (3) ◽

pp. 694 ◽

Cited By ~ 1

Author(s):

Patthranit Narueworanon ◽

Lakkana Laopaiboon ◽

Niphaphat Phukoetphim ◽

Pattana Laopaiboon

Keyword(s):

Calcium Carbonate ◽

Low Cost ◽

Nitrogen Sources ◽

Clostridium Beijerinckii ◽

Sugarcane Molasses ◽

Butanol Production ◽

Gas Stripping ◽

Box Behnken Design ◽

Butanol Fermentation

Low-cost nitrogen sources, i.e., dried spent yeast (DSY), rice bran (RB), soybean meal (SM), urea and ammonium sulfate were used for batch butanol fermentation from sugarcane molasses by Clostridium beijerinckii TISTR 1461 under anaerobic conditions. Among these five low-cost nitrogen sources, DSY at 1.53 g/L (nitrogen content equal to that of 1 g/L of yeast extract) was found to be the most suitable. At an initial sugar level of 60 g/L, the maximum butanol concentration (PB), productivity (QB) and yield (YB/S) were 11.19 g/L, 0.23 g/L·h and 0.31 g/g, respectively. To improve the butanol production, the concentrations of initial sugar, DSY and calcium carbonate were varied using response surface methodology (RSM) based on Box–Behnken design. It was found that the optimal conditions for high butanol production were initial sugar, 50 g/L; DSY, 6 g/L and calcium carbonate, 6.6 g/L. Under these conditions, the highest experimental PB, QB and YB/S values were 11.38 g/L, 0.32 g/L·h and 0.40 g/g, respectively with 50% sugar consumption (SC). The PB with neither DSY nor CaCO3 was only 8.53 g/L. When an in situ gas stripping system was connected to the fermenter to remove butanol produced during the fermentation, the PB was increased to 15.33 g/L, whereas the YB/S (0.39 g/g) was not changed. However, the QB was decreased to 0.21 g/L·h with 75% SC.

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Butanol production from wheat straw by simultaneous saccharification and fermentation using Clostridium beijerinckii: Part II—Fed-batch fermentation

Biomass and Bioenergy ◽

10.1016/j.biombioe.2007.07.005 ◽

2008 ◽

Vol 32 (2) ◽

pp. 176-183 ◽

Cited By ~ 76

Author(s):

Nasib Qureshi ◽

Badal C. Saha ◽

Michael A. Cotta

Keyword(s):

Wheat Straw ◽

Batch Fermentation ◽

Simultaneous Saccharification And Fermentation ◽

Clostridium Beijerinckii ◽

Butanol Production ◽

Fed Batch ◽

Fed Batch Fermentation ◽

Simultaneous Saccharification

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Comparison of different Vetiver grass pretreatment techniques and their impact on immobilized butanol production by Clostridium beijerinckii TISTR 1461

Cellulose ◽

10.1007/s10570-021-04101-4 ◽

2021 ◽

Author(s):

Fuangfa Srisuk ◽

Piyawat Chinwatpaiboon ◽

Thanaphat Atjayutpokin ◽

Akarin Boonsombuti ◽

Ancharida Savarajara ◽

...

Keyword(s):

Clostridium Beijerinckii ◽

Vetiver Grass ◽

Butanol Production

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Developing a coculture for enhanced butanol production by Clostridium beijerinckii and Saccharomyces cerevisiae

Bioresource Technology Reports ◽

10.1016/j.biteb.2019.03.006 ◽

2019 ◽

Vol 6 ◽

pp. 223-228 ◽

Cited By ~ 10

Author(s):

Jiwen Wu ◽

Lili Dong ◽

Chunshuang Zhou ◽

Bingfeng Liu ◽

Liping Feng ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Clostridium Beijerinckii ◽

Butanol Production

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Effective continuous acetone–butanol–ethanol production with full utilization of cassava by immobilized symbiotic TSH06

Biotechnology for Biofuels ◽

10.1186/s13068-019-1561-1 ◽

2019 ◽

Vol 12 (1) ◽

Author(s):

Zhangnan Lin ◽

Hongjuan Liu ◽

Jing Wu ◽

Petra Patakova ◽

Barbora Branska ◽

...

Keyword(s):

Large Scale ◽

Immobilized Cells ◽

Continuous Fermentation ◽

Abe Fermentation ◽

Human Beings ◽

Butanol Production ◽

System A ◽

Glucose System ◽

Acetone Butanol Ethanol ◽

Full Utilization

Abstract Background Butanol production by fermentation has recently attracted increasingly more attention because of its mild reaction conditions and environmentally friendly properties. However, traditional feedstocks, such as corn, are food supplies for human beings and are expensive and not suitable for butanol production at a large scale. In this study, acetone, butanol, and ethanol (ABE) fermentation with non-pretreated cassava using a symbiotic TSH06 was investigated. Results In batch fermentation, the butanol concentration of 11.6 g/L was obtained with a productivity of 0.16 g/L/h, which was similar to that obtained from glucose system. A full utilization system of cassava was constructed to improve the fermentation performance, cassava flour was used as the substrate and cassava peel residue was used as the immobilization carrier. ABE fermentation with immobilized cells resulted in total ABE and butanol concentrations of 20 g/L and 13.3 g/L, which were 13.6% and 14.7% higher, respectively, than those of free cells. To further improve the solvent productivity, continuous fermentation was conducted with immobilized cells. In single-stage continuous fermentation, the concentrations of total ABE and butanol reached 9.3 g/L and 6.3 g/L with ABE and butanol productivities of 1.86 g/L/h and 1.26 g/L/h, respectively. In addition, both of the high product concentration and high solvent productivity were achieved in a three-stage continuous fermentation. The ABE productivity and concentration was 1.12 g/L/h and 16.8 g/L, respectively. Conclusions The results indicate that TSH06 could produce solvents from cassava effectively. This study shows that ABE fermentation with cassava as a substrate could be an efficient and economical method of butanol production.

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Milling byproducts are an economically viable substrate for butanol production using clostridial ABE fermentation

Applied Microbiology and Biotechnology ◽

10.1007/s00253-020-10882-8 ◽

2020 ◽

Vol 104 (20) ◽

pp. 8679-8689

Author(s):

Nils Thieme ◽

Johanna C. Panitz ◽

Claudia Held ◽

Birgit Lewandowski ◽

Wolfgang H. Schwarz ◽

...

Keyword(s):

Clostridium Beijerinckii ◽

Abe Fermentation ◽

Liquid Fuels ◽

Butanol Production ◽

Enzymatic Pretreatment ◽

Profitability Analysis ◽

Key Points ◽

Wide Range ◽

Wheat Middlings ◽

Acetone Butanol Ethanol

Abstract Butanol is a platform chemical that is utilized in a wide range of industrial products and is considered a suitable replacement or additive to liquid fuels. So far, it is mainly produced through petrochemical routes. Alternative production routes, for example through biorefinery, are under investigation but are currently not at a market competitive level. Possible alternatives, such as acetone-butanol-ethanol (ABE) fermentation by solventogenic clostridia are not market-ready to this day either, because of their low butanol titer and the high costs of feedstocks. Here, we analyzed wheat middlings and wheat red dog, two wheat milling byproducts available in large quantities, as substrates for clostridial ABE fermentation. We could identify ten strains that exhibited good butanol yields on wheat red dog. Two of the best ABE producing strains, Clostridium beijerinckii NCIMB 8052 and Clostridium diolis DSM 15410, were used to optimize a laboratory-scale fermentation process. In addition, enzymatic pretreatment of both milling byproducts significantly enhanced ABE production rates of the strains C. beijerinckii NCIMB 8052 and C. diolis DSM 15410. Finally, a profitability analysis was performed for small- to mid-scale ABE fermentation plants that utilize enzymatically pretreated wheat red dog as substrate. The estimations show that such a plant could be commercially successful. Key points • Wheat milling byproducts are suitable substrates for clostridial ABE fermentation. • Enzymatic pretreatment of wheat red dog and middlings increases ABE yield. • ABE fermentation plants using wheat red dog as substrate are economically viable.

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