scholarly journals Evaluation of a New Cerium Oxide-Bismuth Oxide-Based Nanobiocomposite as a Biocatalyst for Biodiesel Production

Processes ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 2012
Author(s):  
Anam Shahzadi ◽  
Muhammad Waseem Mumtaz ◽  
Hamid Mukhtar ◽  
Sadia Akram ◽  
Tooba Touqeer ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Methyl Ester ◽  
Cerium Oxide ◽  
Vegetable Oils ◽  
Bismuth Oxide ◽  
Immobilized Lipase ◽  
Acid Methyl Ester ◽  
Biodiesel Production ◽  
Gas Chromatography Mass Spectrometry ◽  
Acid Methyl

Biodiesel is a promising renewable energy source that can be used together with other biofuels to help meet the growing energy needs of the rapidly increasing global population in an environmentally friendly way. In search for new and more efficient biodiesel production methods, this work reports on the synthesis and use of a novel biocatalyst that can function in a broader range of pH and temperature conditions, while producing high biodiesel yields from vegetable oils. Biodiesel was synthesized by transesterification of non-edible Eruca sativa oil using a lipase from Aspergillus niger that was immobilized on cerium oxide bismuth oxide nanoparticles. The synthesized nanoparticles were first grafted with polydopamine which facilitated the subsequent anchoring of the enzyme on the nanoparticle support. The enzyme activity, pH and temperature stability, and reusability of the immobilized lipase were superior to those of the free enzyme. Following response surface methodology optimization, the highest biodiesel yield of 90.6% was attained using 5 wt% biocatalyst, methanol to oil ratio of 6:1, reaction temperature of 40 °C, pH of 7, and reaction time of 60 h. The produced biodiesel was characterized by Fourier transform infrared spectroscopy and its fatty acid methyl ester composition was determined by gas chromatography-mass spectrometry. Erucic acid methyl ester was identified as the major component in biodiesel, with 47.7 wt% of the total fatty acid methyl esters content. The novel nanobiocatalyst (Bi2O3·CeO2@[email protected]) has the potential to produce high biodiesel yields from a variety of vegetable oils.

scholarly journals Fatty acid methyl ester analysis of Aspergillus fumigatus isolated from fruit pulps for biodiesel production using GC-MS spectrometry

Bioengineered ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 408-415 ◽  
Author(s):  
Ferruh Asci ◽  
Busra Aydin ◽  
Gulderen Uysal Akkus ◽  
Arzu Unal ◽  
Sevim Feyza Erdogmus ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Methyl Ester ◽  
Aspergillus Fumigatus ◽  
Fatty Acid Methyl Ester ◽  
Acid Methyl Ester ◽  
Biodiesel Production ◽  
Acid Methyl

In situ production of fatty acid methyl ester from low quality rice bran: An economical route for biodiesel production

Fuel ◽  
2010 ◽  
Vol 89 (7) ◽  
pp. 1475-1479 ◽  
Author(s):  
Hong Lei ◽  
Xuefeng Ding ◽  
Hongxi Zhang ◽  
Xue Chen ◽  
Yunling Li ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Methyl Ester ◽  
Fatty Acid Methyl Ester ◽  
Rice Bran ◽  
Acid Methyl Ester ◽  
Biodiesel Production ◽  
Acid Methyl ◽  
In Situ Production

scholarly journals Fatty Acid Methyl Ester (FAME) Profiling Identifies Carbapenemase-Producing Klebsiella pneumoniae Belonging to Clonal Complex 258

Separations ◽  
2019 ◽  
Vol 6 (2) ◽  
pp. 32
Author(s):  
Christiaan A. Rees ◽  
Marco Beccaria ◽  
Flavio A. Franchina ◽  
Jane E. Hill ◽  
Giorgia Purcaro
Keyword(s):  
Fatty Acid ◽  
Methyl Ester ◽  
Klebsiella Pneumoniae ◽  
Fatty Acid Methyl Ester ◽  
Learning Algorithm ◽  
Lipid Fraction ◽  
Acid Methyl Ester ◽  
Gas Chromatography Mass Spectrometry ◽  
Bacterial Cells ◽  
Acid Methyl

Carbapenem-resistant Klebsiella pneumoniae (CRKP) is one of the most extensively antibiotic-resistant pathogens encountered in the clinical setting today. A few studies to-date suggest that CRKP and carbapenem-susceptible K. pneumoniae (CSKP) differ from one another not only with respect to their underlying genetics, but also their transcriptomic and metabolomic fingerprints. Within this context, we characterize the fatty acid methyl ester (FAME) profiles of these pathogens in vitro. Specifically, we evaluated the FAME profiles of six Klebsiella pneumoniae carbapenemase (KPC)-producing isolates belonging to the CC258 lineage (KPC+/258+), six KPC-producing isolates belonging to non-CC258 lineages (KPC+/258−), and six non-KPC-producing isolates belonging to non-CC258 lineages (KPC−/258−). We utilized a single-step sample preparation method to simultaneously lyse bacterial cells and transesterify the lipid fraction, and identified 14 unique FAMEs using gas chromatography-mass spectrometry. The machine learning algorithm Random Forest identified four FAMEs that were highly discriminatory between CC258 and non-CC258 isolates (9(Z)-octadecenoate, 2-phenylacetate, pentadecanoate, and hexadecanoate), of which three were also significantly different in relative abundance between these two groups. These findings suggest that distinct differences exist between CC258 and non-CC258 K. pneumoniae isolates with respect to the metabolism of both fatty acids and amino acids, a hypothesis that is supported by previously-acquired transcriptomic data.

scholarly journals Fatty Acid Methyl Esters of the Aerophytic Cave Alga Coccomyxa subglobosa as a Source for Biodiesel Production

Energies ◽  
2020 ◽  
Vol 13 (24) ◽  
pp. 6494
Author(s):  
Joanna Czerwik-Marcinkowska ◽  
Katarzyna Gałczyńska ◽  
Jerzy Oszczudłowski ◽  
Andrzej Massalski ◽  
Jacek Semaniak ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Large Scale ◽  
Methyl Esters ◽  
Acid Methyl Ester ◽  
Biodiesel Production ◽  
Gas Chromatography Mass Spectrometry ◽  
Confocal Laser ◽  
Algal Culture ◽  
Acid Methyl ◽  
Lipid Species

The microscopic alga Coccomyxa subglobosa, collected from the Głowoniowa Nyża Cave (Tatra Mountains, Poland), is a source of fatty acids (FAs) that could be used for biodiesel production. FAs from subaerial algae have unlimited availability because of the ubiquity of algae in nature. Algal culture was carried out under laboratory conditions and algal biomass was measured during growth phase, resulting in 5 g of dry weight (32% oil). The fatty acid methyl ester (FAME) profile was analyzed by means of gas chromatography–mass spectrometry (GC–MS). The presence of lipids and chloroplasts in C. subglobosa was demonstrated using GC–MS and confocal laser microscopy. Naturally occurring FAMEs contained C12–C24 compounds, and methyl palmitate (28.5%) and methyl stearate (45%) were the predominant lipid species. Aerophytic algae could be an important component of biodiesel production, as they are omnipresent and environmentally friendly, contain more methyl esters than seaweed, and can be easily produced on a large scale.

scholarly journals BIODIESEL FROM NYAMPLUNG (CALOPHYLLUM INOPHYLLUM) SEED OIL WITH A TRANSESTERIFICATION PROCESS USING A CONTINOUS FLOW SYSTEM IN THE REACTOR

Tibuana ◽  
2019 ◽  
Vol 2 (02) ◽  
pp. 36-39
Author(s):  
Yanatra budi Pramana
Keyword(s):  
Fatty Acid ◽  
Methyl Ester ◽  
Fatty Acid Methyl Ester ◽  
Reaction Temperature ◽  
Seed Oil ◽  
Flow System ◽  
Acid Methyl Ester ◽  
Biodiesel Production ◽  
Calophyllum Inophyllum ◽  
Acid Methyl

Biodiesel production from calophyllum inophyllum be obtained via esterification and transesterification,  using two continuous reactors with catalyst NaOH at a concentration (0.4 ;0.6,0.8; 1) wt%,  calophyllum inophyllum seed oil and reaction temperature (40, 50,60)oC. This research was to determine the effect of catalyst amount and reaction temperature on transesterification of calophyllum inophyllum seed toward yield Fatty Acid Methyl Ester . The last step is analysis  yield of  Fatty Acid Methyl Ester with Gas Chromatography (GC). The highest yield is obtained on the amount of 1% NaOH catalyst and at a temperature of 60oC with   yield of  87.036%.

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