scholarly journals Polymethacrylate Coated Electrospun PHB Fibers as a Functionalized Platform for Bio-Diagnostics: Confirmation Analysis on the Presence of Immobilized IgG Antibodies against Dengue Virus

Sensors ◽  
2017 ◽  
Vol 17 (10) ◽  
pp. 2292 ◽  
Author(s):  
Samira Hosseini ◽  
Pedram Azari ◽  
Martín Jiménez-Moreno ◽  
Aida Rodriguez-Garcia ◽  
Belinda Pingguan-Murphy ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Igg Antibodies ◽  
Confirmation Analysis

scholarly journals Evaluation of VIDAS® Diagnostic Assay Prototypes Detecting Dengue Virus NS1 Antigen and Anti-Dengue Virus IgM and IgG Antibodies

Diagnostics ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1228
Author(s):  
Somphavanh Somlor ◽  
Ludovic Brossault ◽  
Marc Grandadam
Keyword(s):  
Dengue Virus ◽  
High Sensitivity ◽  
Denv Infection ◽  
Diagnostic Assay ◽  
Igg Antibodies ◽  
Rt Pcr ◽  
Igm Elisa ◽  
Ns1 Antigen ◽  
Adults And Children ◽  
Automated Platform

Dengue is a serious tropical disease caused by the mosquito-borne dengue virus (DENV). Performant, rapid, and easy-to-use assays are needed for the accurate diagnosis of acute DENV infection. We evaluated the performance of three prototype assays developed for the VIDAS® automated platform to detect dengue NS1 antigen and anti-dengue IgM and IgG antibodies. Positive and negative agreement with competitor enzyme-linked immunosorbent assays (ELISA) and rapid diagnostic tests (RDT) was evaluated in 91 Lao patients (57 adults, 34 children) with acute DENV infection. The VIDAS® NS1 assay showed the best overall agreement (95.6%) with the competitor NS1 ELISA. Both VIDAS® NS1 and NS1 ELISA assays also demonstrated high sensitivity relative to DENV RNA RT-PCR set as gold standard (85.7% and 83.9%, respectively). In contrast, NS1 RDT was less sensitive relative to DENV RNA RT-PCR (72.7%). The overall agreement of VIDAS® IgM and IgG assays with the competitor assays was moderate (72.5% for IgM ELISA, 76.9% for IgG ELISA, and 68.7% for IgM and IgG RDT). In most analyses, test agreements of the VIDAS® assays were comparable in adults and children. Altogether, the VIDAS® dengue prototypes performed very well and appear to be suitable for routine detection of dengue NS1 antigen and anti-dengue IgM/IgG antibodies.

Patterns of dengue virus IgM and IgG antibodies in suspected cases of dengue in Jamaica, 2003–2006

2009 ◽  
Vol 18 (1-2) ◽  
pp. 29-34 ◽  
Author(s):  
Michelle G. Brown ◽  
Ivan E. Vickers ◽  
Rose Alba Salas ◽  
Monica Fisher Smikle
Keyword(s):  
Dengue Virus ◽  
Igg Antibodies

scholarly journals Enzyme-linked immunoassay for dengue virus IgM and IgG antibodies in serum and filter paper blood

2006 ◽  
Vol 6 (1) ◽  
Author(s):  
Thanh Nga T Tran ◽  
Peter J de Vries ◽  
Lan Phuong Hoang ◽  
Giao T Phan ◽  
Hung Q Le ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Filter Paper ◽  
Igg Antibodies

scholarly journals Seroprevalence of Asymptomatic Dengue Virus Infection and Its Antibodies Among Healthy/Eligible Saudi Blood Donors: Findings From Holy Makkah City

2017 ◽  
Vol 8 ◽  
pp. 1178122X1769126 ◽  
Author(s):  
Ahmed M Ashshi ◽  
Saad Alghamdi ◽  
Adel G El-Shemi ◽  
Sabir Almdani ◽  
Bassem Refaat ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Dengue Virus ◽  
Blood Donors ◽  
Denv Infection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Dengue Disease ◽  
High Prevalence ◽  
Ns1 Antigen ◽  
Makkah City

Background: Threat to blood transfusion–transmitted dengue virus (DENV) and its antibodies has recently emerged worldwide. Dengue fever is an endemic disease in Saudi Arabia, particularly in its Western region. The aim of this study was to estimate the seroprevalence of asymptomatic DENV infection and its antibodies among eligible Saudi blood donors. Methods: Serum samples from 910 healthy/eligible adult male Saudi blood donors, who reside in Holy Makkah City of Saudi Arabia, were collected between March 2015 and August 2016 and screened for the detection of DENV nonstructural protein 1 (NS1) antigen and anti-DENV IgM and IgG antibodies using commercial enzyme-linked immunosorbent assay kits (Panbio, Brisbane, QLD, Australia). Results: Among the tested donors, 48 (5.3%) were seropositive for DENV-NS1 antigen, whereas 50 (5.5%) and 354 (38.9%) were seropositive for anti-DENV IgM and IgG antibodies, respectively. Seropositivity for DENV-NS1 antigen and/or anti-DENV IgM antibody among the tested donors reflects their ongoing asymptomatic viremic infectious stage with DENV during their donation time, whereas high prevalence of anti-DENV IgG seropositivity reflects the high endemicity of dengue disease in this region of Saudi Arabia. Conclusions: These results show high prevalence of asymptomatic DENV infection and its antibodies among Saudi blood donors, raising the importance of establishing blood screening for dengue disease at different blood donation services and units in Saudi Arabia to improve the guarantee of blood transfusions and to control DENV dissemination.

scholarly journals Immunological Cross-Reactivity to Dengue Virus among Persons with Neuroinvasive West Nile Virus Infection

2022 ◽  
Author(s):  
Vanessa N Raabe ◽  
Muktha S Natrajan ◽  
Christopher M Huerta ◽  
Yongxian Xu ◽  
Lilin Lai ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Virus Infection ◽  
Dengue Virus ◽  
West Nile Virus Infection ◽  
Cross Reactivity ◽  
Igg Antibodies ◽  
West Nile ◽  
Dengue Viruses ◽  
Antibody Dependent Enhancement

Antibody dependent enhancement has been well described between Zika and dengue viruses, but is poorly characterized between West Nile and dengue viruses. We demonstrate that neuroinvasive West Nile virus infection leads to the development of non-neutralizing, cross-reactive IgG antibodies to dengue and Zika viruses capable of causing antibody dependent enhancement in vitro of dengue virus and leads to the formation of flavivirus cross-reactive memory B cells in some patients.

scholarly journals Evaluation of VIDAS® diagnostic assay prototypes detecting dengue virus NS1 antigen and anti-dengue virus IgM and IgG antibodies

2021 ◽  
Author(s):  
Sompavanh Somlor ◽  
Ludovic Brossault ◽  
Marc Grandadam
Keyword(s):  
Dengue Virus ◽  
High Sensitivity ◽  
Denv Infection ◽  
Diagnostic Assay ◽  
Igg Antibodies ◽  
Rt Pcr ◽  
Ns1 Antigen ◽  
Comparable Performance ◽  
Adults And Children ◽  
Automated Platform

AbstractDengue is a tropical disease caused by the mosquito-borne dengue virus (DENV) and affecting an estimated 96 million people each year. Performant, rapid and easy-to-use assays are needed for the accurate diagnosis of acute DENV infection. This study evaluated the performance of three prototype assays developed for the VIDAS® automated platform to detect dengue NS1 antigen and anti-dengue IgM and IgG antibodies. Positive and negative agreement with competitor enzyme-linked immunosorbent assays (ELISA) and rapid diagnostic tests (RDT) was evaluated in 91 Lao patients (57 adults, 34 children) with acute DENV infection. The VIDAS® NS1 assay showed the best overall agreement (95.6%; 95% confidence interval [CI]: 89.1-98.8%) with the competitor NS1 ELISA (Focus Diagnostics). Both assays also demonstrated high sensitivity relative to DENV RNA real-time RT-PCR set as gold standard (85.7% [95% CI: 74.3-92.6%] and 83.9% [95% CI: 72.2-91.3%], respectively). Concordance of the VIDAS® results was overall higher with the competitor ELISA than with the RDT, suggesting a better performance of VIDAS® assays over RDT. The overall agreement of VIDAS® IgM and IgG assays with the competitor ELISA (Panbio/Abbott) was moderate (72.5%, 95% CI [62.6-80.6%] for IgM; 76.9%, 95% CI [67.3-84.4%] for IgG), highlighting differences in sensitivity and/or specificity between these assays. In all comparisons, test agreements did not differ significantly between adults and children, indicating comparable performance of the VIDAS® assays in both populations. Altogether, the VIDAS® dengue prototypes performed very well and appear to be suitable for routine detection of dengue NS1 antigen and anti-dengue IgM/IgG antibodies.

scholarly journals Seroprevalence of three emerging arboviral infections in Kuwaiti nationals

2021 ◽  
Vol 9 (3) ◽  
pp. 266-273
Author(s):  
A. S. Pacsa ◽  
U. C. Chaturvedi ◽  
A. S. Mustafa
Keyword(s):  
High Risk ◽  
Health Risk ◽  
Dengue Virus ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Major Health ◽  
Specific Antibodies ◽  
Igm Antibodies ◽  
Hanta Viruses

Diseases caused by dengue, s and fly fever and hanta viruses pose a major health risk in many countries. We determined the threat of these arboviral infections through a serologic using enzyme linked immunosorbent assay [ELISA] based tests. Hantavirus-specific antibodies were also detected using immunofluorescence. Of 499 samples tested for dengue virus IgG antibodies l4% were as positive for dengue positive by all the ELISA tests. Among the 42 showing strong IgG reactivity, only 1 was positive for dengue virus IgM antibodies. All samples tested for IgG antibodies to s and fly fever virus were negative. Hantavirus antibodies were detected in 11% of the 46 samples from high-risk individuals. The low prevalences suggest that at present these infections are not a serious problem in Kuwait

scholarly journals Evaluation of a Rapid Immunochromatographic Test for Diagnosis of Dengue Virus Infection

1998 ◽  
Vol 36 (1) ◽  
pp. 234-238 ◽  
Author(s):  
David W. Vaughn ◽  
Ananda Nisalak ◽  
Siripen Kalayanarooj ◽  
Tom Solomon ◽  
Nguyen Minh Dung ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Rapid Test ◽  
Positive Test ◽  
Immunochromatographic Test ◽  
Test Results ◽  
Igg Antibodies ◽  
Virus Infections ◽  
Dengue Virus Infection ◽  
Virus Serotype

A rapid (<7-min) immunochromatographic test for immunoglobulin M (IgM) and IgG antibodies to dengue viruses was evaluated by using hospital admission and discharge sera from 124 patients. The reference laboratory diagnosis was based on the results of virus isolation, hemagglutination-inhibition assay (HAI), and enzyme immunoassay (EIA). By the standard assays, patients experienced primary dengue virus infection (n = 30), secondary dengue virus infection (n = 48), Japanese encephalitis (JE) virus infection (n = 20), or no flavivirus infection (n = 26). The rapid test demonstrated 100% sensitivity in the diagnosis of dengue virus infection and was able to distinguish between primary and secondary dengue virus infections through the separate determinations of IgM and IgG. For all patients with primary dengue virus infection a positive test for IgM to dengue virus and a negative test for IgG to dengue virus were obtained, whereas for 46 of 48 patients (96%) with secondary dengue virus infection, a positive test for IgG to dengue virus with or without a positive test for IgM to dengue virus was obtained. The remaining two patients with secondary dengue virus infection had positive IgM test results and negative IgG test results. Furthermore, the rapid test was positive for patients confirmed to be infected with different dengue virus serotypes (12 infected with dengue virus serotype 1, 4 infected with dengue virus serotype 2, 3 infected with dengue virus serotype 3, and 2 infected with dengue virus serotype 4). The specificity of the test for nonflavivirus infections was 88% (3 of 26 positive), while for JE virus infections the specificity of the test was only 50% (10 of 20). However, most patients with secondary dengue virus infection were positive for both IgM and IgG antibodies to dengue virus, while no patients with JE virus infection had this profile, so cross-reactivity was only a concern for a small proportion of patients with secondary dengue infections. The rapid test demonstrated a good correlation with the reference EIA and HAI and should be useful for the rapid diagnosis of dengue virus infections.

scholarly journals Seroepidemiology of Asymptomatic Dengue Virus Infection in Jeddah, Saudi Arabia

2016 ◽  
Vol 7 ◽  
pp. VRT.S34187 ◽  
Author(s):  
Ghazi A. Jamjoom ◽  
Esam I. Azhar ◽  
Moujahid A. Kao ◽  
Raja M. Radadi
Keyword(s):  
Saudi Arabia ◽  
Virus Infection ◽  
Dengue Virus ◽  
Dengue Fever ◽  
Blood Donors ◽  
Dengue Infection ◽  
Igg Antibodies ◽  
Dengue Virus Infection ◽  
High Exposure ◽  
Dengue Viruses

Background Although virologically confirmed dengue fever has been recognized in Jeddah, Saudi Arabia, since 1994, causing yearly outbreaks, no proper seroepidemiologic studies on dengue virus have been conducted in this region. Such studies can define the extent of infection by this virus and estimate the proportion that may result in disease. The aim of this study was to measure the seroprevalence of past dengue virus infection in healthy Saudi nationals from different areas in the city of Jeddah and to investigate demographic and environmental factors that may increase exposure to infection. Methods Sera were collected from 1984 Saudi subjects attending primary health care centers in six districts of Jeddah. These included general patients of various ages seeking routine vaccinations, antenatal care or treatment of different illnesses excluding fever or suspected dengue. A number of blood donors were also tested. Serum samples were tested by enzyme immunoassay (EIA) for IgG antibodies to dengue viruses 1, 2, 3, 4. A questionnaire was completed for each patient recording various anthropometric data and factors that may indicate possible risk of exposure to mosquito bites and dengue infection. Patients with missing data and those who reported a history of dengue fever were excluded from analysis, resulting in a sample of 1939 patients to be analyzed. Results The overall prevalence of dengue virus infection as measured by anti-dengue IgG antibodies from asymptomatic residents in Jeddah was 47.8% (927/1939) and 37% (68/184) in blood donors. Infection mostly did not result in recognizable disease, as only 19 of 1956 subjects with complete information (0.1%) reported having dengue fever in the past. Anti dengue seropositivity increased with age and was higher in males than females and in residents of communal housing and multistory buildings than in villas. One of the six districts showed significant increase in exposure rate as compared to the others. Availability of public sewage was associated with lower infection at a nearly significant level. No other clear risk factors were identifiable. Infection was not related to travel abroad. Conclusions Our results indicate a relatively high exposure of Jeddah residents to infection by dengue viruses, which must be considered endemic to this region. Infection largely remained asymptomatic or was only associated with minor illness for which patients did not seek treatment. These results call for continued vigilance for clinical cases of dengue that may arise from this wide exposure. They also call for more extensive control efforts to reduce exposure to and transmission of dengue viruses.

scholarly journals Comparison of Six Commercial Diagnostic Tests for the Detection of Dengue Virus Non-Structural-1 Antigen and IgM/IgG Antibodies

2019 ◽  
Vol 39 (6) ◽  
pp. 566-571 ◽  
Author(s):  
Hyeyoung Lee ◽  
Ji Hyeong Ryu ◽  
Hye-Sun Park ◽  
Ki Hyun Park ◽  
Hyunjoo Bae ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Diagnostic Tests ◽  
Igg Antibodies
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