Development of Fluorescent Reagent Based on Ligand Exchange Reaction for the Highly Sensitive and Selective Detection of Dopamine in the Serum

A new fluorescent probe (BDP-Fe2+) was developed for targeting dopamine, with a boron–dipyrromethenyl (BDP) group as the fluorophore and a Fe2+ complex as the ligand exchange site. The free form of BDP-Fe2+ in solution displayed weak fluorescence emission, while it showed strong fluorescence emission after interaction with dopamine due to the release of Fe2+ from BDP-Fe2+, confirming the binding of Fe2+ to dopamine. The increase in fluorescence intensity was concentration-dependent, and a good linear relationship was observed between the fluorescence intensity and dopamine concentration. The detection limit of dopamine by BDP-Fe2+ was 1.1 nM, indicating a 20-fold higher sensitivity than that of previously reported compounds. The reaction of BDP-Fe2+ with dopamine was not affected by the presence of foreign substances, allowing the highly selective detection of dopamine in the human serum sample. The results of this study indicate that the novel compound BDP-Fe2+ is a reliable fluorescent molecular probe for the detection of dopamine and can be widely employed in diverse scientific areas.

Download Full-text

An electrochemical sensor for highly sensitive detection of copper ions based on a new molecular probe Pi-A decorated on graphene

Analytical Methods ◽

10.1039/c6ay03006h ◽

2017 ◽

Vol 9 (4) ◽

pp. 618-624 ◽

Cited By ~ 15

Author(s):

Liuqing Yang ◽

Na Huang ◽

Liyan Huang ◽

Meiling Liu ◽

Haitao Li ◽

...

Keyword(s):

Electrochemical Sensor ◽

Electrochemical Method ◽

Copper Ions ◽

Molecular Probe ◽

Selective Detection ◽

Carbon Electrode ◽

Modified Glassy Carbon Electrode ◽

Reduced Graphene ◽

Highly Sensitive ◽

Highly Sensitive Detection

In this paper, a new electrochemical sensor based on a new fluorescent probe N-(2-(1-(p-tolyl)-1H-phenanthro[9,10-d]imidazol-2-yl)phenyl)picolinamide (Pi-A) decorated reduced graphene oxide (RGO) modified glassy carbon electrode (GCE) was developed for highly selective detection of copper ions using the electrochemical method.

Download Full-text

Highly Sensitive Gold Nanoparticle-Based Colorimetric Sensing of Mercury(II) through Simple Ligand Exchange Reaction in Aqueous Media

ACS Applied Materials & Interfaces ◽

10.1021/am9006963 ◽

2009 ◽

Vol 2 (1) ◽

pp. 292-295 ◽

Cited By ~ 102

Author(s):

Yang-Rae Kim ◽

Rakesh Kumar Mahajan ◽

Jong Seung Kim ◽

Hasuck Kim

Keyword(s):

Exchange Reaction ◽

Gold Nanoparticle ◽

Ligand Exchange ◽

Aqueous Media ◽

Colorimetric Sensing ◽

Ligand Exchange Reaction ◽

Highly Sensitive

Download Full-text

Synthesis and Photochemical Properties of Re(I) Tricarbonyl Complexes Bound to Thione and Thiazole-2-ylidene Ligands

10.26434/chemrxiv.12324875 ◽

2020 ◽

Author(s):

Matthew Stout ◽

Brian Skelton ◽

Alexandre N. Sobolev ◽

Paolo Raiteri ◽

Massimiliano Massi ◽

...

Keyword(s):

Charge Transfer ◽

Excited States ◽

Ligand Exchange ◽

Ligand Substitution ◽

Bidentate Ligand ◽

The Other ◽

Ligand Exchange Reaction ◽

Multiple Products ◽

Ligand Charge Transfer ◽

Photochemical Properties

Three Re(I) tricarbonyl complexes, with general formulation Re(N^L)(CO)3X (where N^L is a bidentate ligand containing a pyridine functionalized in the position 2 with a thione or a thiazol-2-ylidene group and X is either chloro or bromo) were synthesized and their reactivity explored in terms of solvent-dependent ligand substitution, both in the ground and excited states. When dissolved in acetonitrile, the complexes bound to the thione ligand underwent ligand exchange with the solvent resulting in the formation of Re(NCMe)2(CO)3X. The exchange was found to be reversible, and the starting complex was reformed upon removal of the solvent. On the other hand, the complexes appeared inert in dichloromethane or acetone. Conversely, the complex bound to the thiazole-2-ylidene ligand did not display any ligand exchange reaction in the dark, but underwent photoactivated ligand substitution when excited to its lowest metal-to-ligand charge transfer manifold. Photolysis of this complex in acetonitrile generated multiple products, including Re(I) tricarbonyl and dicarbonyl solvato-complexes as well as free thiazole-2-ylidene ligand.

Download Full-text

Recent Advances in Quenchbody, a Fluorescent Immunosensor

Sensors ◽

10.3390/s21041223 ◽

2021 ◽

Vol 21 (4) ◽

pp. 1223

Author(s):

Jinhua Dong ◽

Hiroshi Ueda

Keyword(s):

Fluorescence Intensity ◽

Fluorescent Dyes ◽

Antibody Fragment ◽

Disease Diagnosis ◽

Disease Biomarkers ◽

Highly Sensitive ◽

Physiologically Active Substances ◽

New Type ◽

Antigen Antibody ◽

Active Substances

The detection of viruses, disease biomarkers, physiologically active substances, drugs, and chemicals is of great significance in many areas of our lives. Immunodetection technology is based on the specificity and affinity of antigen–antibody reactions. Compared with other analytical methods such as liquid chromatography coupled with mass spectrometry, which requires a large and expensive instrument, immunodetection has the advantages of simplicity and good selectivity and is thus widely used in disease diagnosis and food/environmental monitoring. Quenchbody (Q-body), a new type of fluorescent immunosensor, is an antibody fragment labeled with fluorescent dyes. When the Q-body binds to its antigen, the fluorescence intensity increases. The detection of antigens by changes in fluorescence intensity is simple, easy to operate, and highly sensitive. This review comprehensively discusses the principle, construction, application, and current progress related to Q-bodies.

Download Full-text