scholarly journals Application of Electrochemical Aptasensors toward Clinical Diagnostics, Food, and Environmental Monitoring: Review

Sensors ◽  
2019 ◽  
Vol 19 (24) ◽  
pp. 5435 ◽  
Author(s):  
Zhanhong Li ◽  
Mona A. Mohamed ◽  
A. M. Vinu Mohan ◽  
Zhigang Zhu ◽  
Vinay Sharma ◽  
...  
Keyword(s):  
Environmental Monitoring ◽  
Biomedical Application ◽  
Analytical Techniques ◽  
Clinical Diagnostics ◽  
Cardiac Biomarkers ◽  
Research Progress ◽  
Electrochemical Biosensors ◽  
Recognition Element ◽  
Clinical Biomarkers ◽  
Electrochemical Biosensing

Aptamers are synthetic bio-receptors of deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) origin selected by the systematic evolution of ligands (SELEX) process that bind a broad range of target analytes with high affinity and specificity. So far, electrochemical biosensors have come up as a simple and sensitive method to utilize aptamers as a bio-recognition element. Numerous aptamer based sensors have been developed for clinical diagnostics, food, and environmental monitoring and several other applications are under development. Aptasensors are capable of extending the limits of current analytical techniques in clinical diagnostics, food, and environmental sample analysis. However, the potential applications of aptamer based electrochemical biosensors are unlimited; current applications are observed in the areas of food toxins, clinical biomarkers, and pesticide detection. This review attempts to enumerate the most representative examples of research progress in aptamer based electrochemical biosensing principles that have been developed in recent years. Additionally, this account will discuss various current developments on aptamer-based sensors toward heavy metal detection, for various cardiac biomarkers, antibiotics detection, and also on how the aptamers can be deployed to couple with antibody-based assays as a hybrid sensing platform. Aptamers can be used in various applications, however, this account will focus on the recent advancements made toward food, environmental, and clinical diagnostic application. This review paper compares various electrochemical aptamer based sensor detection strategies that have been applied so far and used as a state of the art. As illustrated in the literature, aptamers have been utilized extensively for environmental, cancer biomarker, biomedical application, and antibiotic detection and thus have been extensively discussed in this article.

scholarly journals The Role of Peptides in the Design of Electrochemical Biosensors for Clinical Diagnostics

Biosensors ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 246
Author(s):  
Patrick Severin Sfragano ◽  
Giulia Moro ◽  
Federico Polo ◽  
Ilaria Palchetti
Keyword(s):  
Functional Groups ◽  
Synthetic Peptides ◽  
Clinical Diagnostics ◽  
Electrochemical Biosensors ◽  
Practical Application ◽  
Design And Development ◽  
Electrode Surfaces ◽  
Electrochemical Transducers ◽  
Target Analyte

Peptides represent a promising class of biorecognition elements that can be coupled to electrochemical transducers. The benefits lie mainly in their stability and selectivity toward a target analyte. Furthermore, they can be synthesized rather easily and modified with specific functional groups, thus making them suitable for the development of novel architectures for biosensing platforms, as well as alternative labelling tools. Peptides have also been proposed as antibiofouling agents. Indeed, biofouling caused by the accumulation of biomolecules on electrode surfaces is one of the major issues and challenges to be addressed in the practical application of electrochemical biosensors. In this review, we summarise trends from the last three years in the design and development of electrochemical biosensors using synthetic peptides. The different roles of peptides in the design of electrochemical biosensors are described. The main procedures of selection and synthesis are discussed. Selected applications in clinical diagnostics are also described.

scholarly journals Food Safety Analysis Using Electrochemical Biosensors

Foods ◽  
2018 ◽  
Vol 7 (9) ◽  
pp. 141 ◽  
Author(s):  
Geetesh Mishra ◽  
Abbas Barfidokht ◽  
Farshad Tehrani ◽  
Rupesh Mishra
Keyword(s):  
Food Safety ◽  
Food Industry ◽  
Analytical Techniques ◽  
High Specificity ◽  
Quantitative Detection ◽  
Electrochemical Biosensors ◽  
Food Contaminants ◽  
Industry Standard ◽  
Analytical Accuracy ◽  
Analytical Tools

Rapid and precise analytical tools are essential for monitoring food safety and screening of any undesirable contaminants, allergens, or pathogens, which may cause significant health risks upon consumption. Substantial developments in analytical techniques have empowered the analyses and quantitation of these contaminants. However, conventional techniques are limited by delayed analysis times, expensive and laborious sample preparation, and the necessity for highly-trained workers. Therefore, prompt advances in electrochemical biosensors have supported significant gains in quantitative detection and screening of food contaminants and showed incredible potential as a means of defying such limitations. Apart from indicating high specificity towards the target analytes, these biosensors have also addressed the challenge of food industry by providing high analytical accuracy within complex food matrices. Here, we discuss some of the recent advances in this area and analyze the role and contributions made by electrochemical biosensors in the food industry. This article also reviews the key challenges we believe biosensors need to overcome to become the industry standard.

Research progress of SERS on uranyl ions and uranyl compounds: A review

2021 ◽  
Author(s):  
Shaofei Wang ◽  
Jiaolai Jiang ◽  
Xuan He ◽  
Shanli Yang ◽  
Haoxi Wu ◽  
...  
Keyword(s):  
Environmental Monitoring ◽  
Research Progress ◽  
Uranyl Ions ◽  
Uranyl Compounds ◽  
Sensitive Analysis ◽  
Nuclear Emergency

There is an urgent demand for rapid and sensitive analysis of uranyl ions and uranyl compounds in the environmental monitoring and nuclear emergency fields. However, most of the current analytic...

Optical and Electrochemical Biosensors for Express Environmental Monitoring

2003 ◽  
pp. 355-372
Author(s):  
N. F. Starodub ◽  
V. M. Starodub ◽  
A. M. Katzev ◽  
I. A. Levkovetz ◽  
T. L. Dibrova ◽  
...  
Keyword(s):  
Environmental Monitoring ◽  
Electrochemical Biosensors

scholarly journals Label-Free Electrochemical Biosensors for the Determination of Flaviviruses: Dengue, Zika, and Japanese Encephalitis

Sensors ◽  
2020 ◽  
Vol 20 (16) ◽  
pp. 4600 ◽  
Author(s):  
Ekaterina Khristunova ◽  
Elena Dorozhko ◽  
Elena Korotkova ◽  
Bohumil Kratochvil ◽  
Vlastimil Vyskocil ◽  
...  
Keyword(s):  
Japanese Encephalitis ◽  
Electrode Materials ◽  
Biological Fluids ◽  
Research Progress ◽  
Immunological Methods ◽  
Label Free ◽  
Viral Pathogens ◽  
Electrochemical Biosensing ◽  
Wide Range

A highly effective way to improve prognosis of viral infectious diseases and to determine the outcome of infection is early, fast, simple, and efficient diagnosis of viral pathogens in biological fluids. Among a wide range of viral pathogens, Flaviviruses attract a special attention. Flavivirus genus includes more than 70 viruses, the most familiar being dengue virus (DENV), Zika virus (ZIKV), and Japanese encephalitis virus (JEV). Haemorrhagic and encephalitis diseases are the most common severe consequences of flaviviral infection. Currently, increasing attention is being paid to the development of electrochemical immunological methods for the determination of Flaviviruses. This review critically compares and evaluates recent research progress in electrochemical biosensing of DENV, ZIKV, and JEV without labelling. Specific attention is paid to comparison of detection strategies, electrode materials, and analytical characteristics. The potential of so far developed biosensors is discussed together with an outlook for further development in this field.

scholarly journals Clinical Applications of Visual Plasmonic Colorimetric Sensing

Sensors ◽  
2020 ◽  
Vol 20 (21) ◽  
pp. 6214
Author(s):  
Elba Mauriz
Keyword(s):  
Point Of Care ◽  
Clinical Diagnostics ◽  
Localized Surface Plasmon ◽  
Visible Range ◽  
Future Perspectives ◽  
Colorimetric Analysis ◽  
Colorimetric Sensing ◽  
Size Dependent ◽  
Clinical Biomarkers ◽  
Naked Eye Detection

Colorimetric analysis has become of great importance in recent years to improve the operationalization of plasmonic-based biosensors. The unique properties of nanomaterials have enabled the development of a variety of plasmonics applications on the basis of the colorimetric sensing provided by metal nanoparticles. In particular, the extinction of localized surface plasmon resonance (LSPR) in the visible range has permitted the exploitation of LSPR colorimetric-based biosensors as powerful tools for clinical diagnostics and drug monitoring. This review summarizes recent progress in the biochemical monitoring of clinical biomarkers by ultrasensitive plasmonic colorimetric strategies according to the distance- or the morphology/size-dependent sensing modes. The potential of colorimetric nanosensors as point of care devices from the perspective of naked-eye detection is comprehensively discussed for a broad range of analytes including pharmaceuticals, proteins, carbohydrates, nucleic acids, bacteria, and viruses such as Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The practical suitability of plasmonic-based colorimetric assays for the rapid visual readout in biological samples, considering current challenges and future perspectives, is also reviewed.

scholarly journals Miniaturization of analytical systems

1998 ◽  
Vol 44 (9) ◽  
pp. 2008-2014 ◽  
Author(s):  
Larry J Kricka
Keyword(s):  
Self Assembly ◽  
New Technologies ◽  
Analytical Techniques ◽  
Sample Volume ◽  
Clinical Diagnostics ◽  
Key Issues ◽  
User Friendly ◽  
Near Future ◽  
Long Term Trend

Abstract Miniaturization has been a long-term trend in clinical diagnostics instrumentation. Now a range of new technologies, including micromachining and molecular self-assembly, are providing the means for further size reduction of analyzers to devices with micro- to nanometer dimensions and submicroliter volumes. Many analytical techniques (e.g., mass spectrometry and electrophoresis) have been successfully implemented on microchips made from silicon, glass, or plastic. The new impetus for miniaturization stems from the perceived benefits of faster, easier, less costly, and more convenient analyses and by the needs of the pharmaceutical industry for microscale, massively parallel drug discovery assays. Perfecting a user-friendly interface between a human and a microchip and determining the realistic lower limit for sample volume are key issues in the future implementation of these devices. Resolution of these issues will be important for the long-term success of microminiature analyzers; in the meantime, the scope, diversity, and rate of progress in the development of these devices promises products in the near future.

DNA electrochemical biosensors for environmental monitoring. A review

1997 ◽  
Vol 347 (1-2) ◽  
pp. 1-8 ◽  
Author(s):  
J. Wang ◽  
G. Rivas ◽  
X. Cai ◽  
E. Palecek ◽  
P. Nielsen ◽  
...  
Keyword(s):  
Environmental Monitoring ◽  
Electrochemical Biosensors
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