Surface Display Technology for Biosensor Applications: A Review

Surface display is a recombinant technology that expresses target proteins on cell membranes and can be applied to almost all types of biological entities from viruses to mammalian cells. This technique has been used for various biotechnical and biomedical applications such as drug screening, biocatalysts, library screening, quantitative assays, and biosensors. In this review, the use of surface display technology in biosensor applications is discussed. In detail, phage display, bacterial surface display of Gram-negative and Gram-positive bacteria, and eukaryotic yeast cell surface display systems are presented. The review describes the advantages of surface display systems for biosensor applications and summarizes the applications of surface displays to biosensors.

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Bacterial surface display (bacterial display, bacteria display, microbial cell surface display)

The Dictionary of Genomics, Transcriptomics and Proteomics ◽

10.1002/9783527678679.dg00871 ◽

2015 ◽

pp. 1-1

Keyword(s):

Cell Surface ◽

Surface Display ◽

Cell Surface Display ◽

Microbial Cell ◽

Bacterial Display ◽

Bacterial Surface ◽

Bacterial Surface Display

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Novel bacterial surface display systems based on outer membrane anchoring elements from marine bacterium Vibrio anguillarum

Journal of Biotechnology ◽

10.1016/j.jbiotec.2008.07.1305 ◽

2008 ◽

Vol 136 ◽

pp. S555

Author(s):

Zhao Yang ◽

Qin Liu ◽

Qiyao Wang ◽

Yuanxing Zhang

Keyword(s):

Outer Membrane ◽

Marine Bacterium ◽

Surface Display ◽

Vibrio Anguillarum ◽

Bacterial Surface ◽

Bacterial Surface Display ◽

Membrane Anchoring ◽

Display Systems

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Ice nucleation protein as a bacterial surface display protein

Archives of Biological Sciences ◽

10.2298/abs1104943s ◽

2011 ◽

Vol 63 (4) ◽

pp. 943-948 ◽

Cited By ~ 6

Author(s):

Mohammed Sarhan

Keyword(s):

Recombinant Proteins ◽

Surface Display ◽

Ice Nucleation ◽

Display System ◽

Ice Nucleation Protein ◽

Bacterial Surface ◽

Sorting Signals ◽

Bacterial Surface Display ◽

Display Technology ◽

Anchoring Motif

Surface display technology can be defined as that phenotype (protein or peptide) which is linked to a genotype (DNA or RNA) through an appropriate anchoring motif. A bacterial surface display system is based on expressing recombinant proteins fused to sorting signals (anchoring motifs) that direct their incorporation on the cell surface.

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Construction of a Yeast Cell-Surface Display System and Expression of Trametes sp. laccase

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.347-353.3635 ◽

2011 ◽

Vol 347-353 ◽

pp. 3635-3640 ◽

Cited By ~ 1

Author(s):

Jian Zhang Lu ◽

Qin Guo ◽

Mei Lin Cui ◽

Lu Yang ◽

Shan Shan Du ◽

...

Keyword(s):

Cell Surface ◽

Surface Display ◽

Cell Surface Display ◽

Heterologous Proteins ◽

Secretion Signal ◽

Yeast Cell Surface ◽

Gal1 Promoter ◽

Genes Encoding ◽

Display Technology ◽

A Cell

Laccases (1.10.3.2, p-diphenol: dioxygen oxidoreductases) is a family of blue copper-containing oxidases that are commonly found in bacteria, fungi and plants. It is able to oxidize and degrade a variety of aromatic compounds and other organic compounds. Due to this ability, laccases can serve environmental bioremediation processes and industrial purposes. Cell-surface display of enzymes is one of the most attractive applications in yeast. It is a effective utilization to construct the whole cell biocatalyst. The cDNA sequence of Trametes sp. C30 LAC3 was optimized and synthesized according to the codon bias of Saccharomyces Italic textcerevisiae, because codon optimization has been proved to be effective to maximize production of heterologous proteins in yeast. The genes encoding galactokinase (GAL1) promoter, α-mating factor 1 (MFα1) pre-pro secretion signal, fully codon-optimized LAC3, the 320 amino acids of C terminal of α-agglutinin, alcohol dehydrogenase (ADH1) terminator and kanMX cassette were amplified and cloned into YEplac181 to construct a cell-surface display vector called pGMAAK-lac3 with α-agglutinin as an anchor. Then pGMAAK-lac3 was transformed into S. cerevisiae. The results show LAC3 was immobilized and actively expressed on S. cerevisiae. However, the substrate specifity and activity were obviously changed. The displayed LAC3 lost the activity to phenolic substrate (guaiacol) and its activity to non-phenolic substrate (ABTS) was greatly reduced. To our knowledge, this was the first attempt to construct and express laccase through cell-surface display technology.

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