scholarly journals Characterization and Differentiation between Olive Varieties through Electrical Impedance Spectroscopy, Neural Networks and IoT

Sensors ◽  
2020 ◽  
Vol 20 (20) ◽  
pp. 5932
Author(s):  
José Miguel Madueño Luna ◽  
Antonio Madueño Luna ◽  
Rafael E. Hidalgo Fernández

Electrical impedance has shown itself to be useful in measuring the properties and characteristics of agri-food products: fruit quality, moisture content, the germination capacity in seeds or the frost-resistance of fruit. In the case of olives, it has been used to determine fat content and optimal harvest time. In this paper, a system based on the System on Chip (SoC) AD5933 running a 1024-point discrete Fourier transform (DFT) to return the impedance value as a magnitude and phase and which, working together with two ADG706 analog multiplexers and an external programmable clock based on a synthesized DDS in a FPGA XC3S250E-4VQG100C, allows for the impedance measurement in agri-food products with a frequency sweep from 1 Hz to 100 kHz. This paper demonstrates how electrical impedance is affected by the temperature both in freshly picked olives and in those processed in brine and provides a way to characterize cultivars by making use of only the electrical impedance, neural networks (NN) and the Internet of Things (IoT), allowing information to be collected from the olive samples analyzed both on farms and in factories.

Lab on a Chip ◽  
2010 ◽  
Vol 10 (12) ◽  
pp. 1611 ◽  
Author(s):  
Tao Sun ◽  
Emily J. Swindle ◽  
Jane E. Collins ◽  
Judith A. Holloway ◽  
Donna E. Davies ◽  
...  

HortScience ◽  
2000 ◽  
Vol 35 (1) ◽  
pp. 104-107 ◽  
Author(s):  
Phillipa J. Jackson ◽  
F. Roger Harker

Electrical impedance was used to determine the extent of tissue damage that occurred as a result of bruising of apple fruit (Malus ×domestica Borkh, cvs. Granny Smith and Splendour). Impedance measurements were made before and after bruising. Plots of reactance against resistance at 36 spot frequencies between 50 Hz and 1 MHz traced a semicircular arc, which contracted in magnitude after bruising. A number of characteristics of these curves were then related to bruise weight. The change in resistance that occurred as a result of fruit impact (ΔR50Hz) was the best predictor of bruise weight, with r2 values up to 0.71. Before bruising, resistance of fruit was higher in `Splendour' than in `Granny Smith' (P < 0.001), and at 0 °C than at 20 °C (P < 0.001), but was not influenced by fruit weight. The influence of apple cultivar and temperature on electrical impedance may cause difficulties when implementing these measurements in a commercial situation. However, further development of electrical impedance spectroscopy methodologies may result in convenient research techniques for assessing bruise weight without having to wait for browning of the flesh.


Proceedings ◽  
2018 ◽  
Vol 2 (13) ◽  
pp. 1053
Author(s):  
Martin Jaegle ◽  
Hans-Fridtjof Pernau ◽  
Marcus Pfützner ◽  
Mike Benkendorf ◽  
Xinke Li ◽  
...  

Electrical impedance spectroscopy is a widespread characterization method for solids or fluids in industrial applications. We here report on its thermal equivalent, the “thermal impedance spectroscopy”, improved by using a temperature compensation method for temperature dependent thermal measurements using an on-chip reference resistor.


Author(s):  
Zhen Zhu ◽  
Yangye Geng ◽  
Yingying Wang ◽  
Ke Liu ◽  
Zhenxiang Yi ◽  
...  

Microfluidic devices in combination with fluorescent microscopy offer high-resolution and high-content platforms to study single-cell morphology, behavior and dynamic process in replicative aging of budding yeast, Saccharomyces cerevisiae. However, a huge mass of recorded images makes the data processing labor-intensive and time-consuming to determine yeast replicative lifespan (RLS), a primary criterion in yeast aging. To address this limitation and pursue label-free RLS assays, electrical impedance spectroscopy (EIS) that can be easily functionalized through microelectrodes in microfluidic devices, was introduced to monitor cell growth and division of budding yeast. Herein, a microfluidic device integrated with EIS biosensor was proposed to perform in-situ impedance measurement of yeast proliferation in single-cell resolution so as to identify the momentary events of daughter dissection from its mother. Single yeast cells were reliably immobilized at the bottleneck-like traps for continuous culturing, during which daughter cells were effectively detached from their mother cells by hydraulic shear forces. Time-lapse impedance measurement was performed every 2 min to monitor the cellular process including budding, division and dissection. By using the K-means clustering algorithm to analyze a self-defined parameter “Dissection Indicator,” to our knowledge for the first time, the momentary event of a daughter removing from its mother cell was accurately extracted from EIS signals. Thus, the identification of daughter dissection events based on impedance sensing technology has been validated. With further development, this microfluidic device integrated with electrical impedance biosensor holds promising applications in high-throughput, real-time and label-free analysis of budding yeast aging and RLS.


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