scholarly journals Mechanism of Action of Mangifera indica Leaves for Anti-Diabetic Activity

2019 ◽  
Vol 87 (2) ◽  
pp. 13 ◽  
Author(s):  
Dai-Hung Ngo ◽  
Dai-Nghiep Ngo ◽  
Thi Thanh Nhan Vo ◽  
Thanh Sang Vo
Keyword(s):  
Sulfonic Acid ◽  
In Vitro Model ◽  
Amylase Activity ◽  
Mangifera Indica ◽  
Significant Inhibition ◽  
No Production ◽  
Vitro Model ◽  
Cytotoxicity Effects

Diabetes is a major metabolic disorder whose prevalence is increasing daily. Medicinal plants have played an important role in the prevention and treatment of type 2 diabetes via prophylactic and therapeutic management. In this study, Mangifera Indica leaf (MIL) extract was investigated for its promising anti-diabetic activity via an in vitro model. It was found that MIL extract possessed significant inhibition on alpha-amylase activity up to (51.4 ± 2.7)% at a concentration of 200 µg/mL. Moreover, glucose adsorption capacity of MIL was identified at (2.7 ± 0.19) mM glucose/g extract. Furthermore, the extract caused a significant increase in glucose uptake up to (143 ± 9.3)% in LO-2 liver cells. Notably, MIL extract was effective in scavenging (63.3 ± 2.1)% 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and (71.6 ± 4.3)% 2,2-azinobis-3-ethyl benzothiazoline-6-sulfonic acid (ABTS)+ radicals and inhibiting (66 ± 4.9)% NO production from RAW264.7 cells without any cytotoxicity effects. Accordingly, M. indica leaves are suggested as a promising material for development of hypoglycemic products.

scholarly journals 3D Environment Is Required In Vitro to Demonstrate Altered Bone Metabolism Characteristic for Type 2 Diabetics

2021 ◽  
Vol 22 (6) ◽  
pp. 2925
Author(s):  
Victor Häussling ◽  
Romina H Aspera-Werz ◽  
Helen Rinderknecht ◽  
Fabian Springer ◽  
Christian Arnscheidt ◽  
...  
Keyword(s):  
Bone Metabolism ◽  
In Vitro Model ◽  
Bone Diseases ◽  
3D Environment ◽  
Type 2 Diabetics ◽  
Mineralized Matrix ◽  
Vitro Model

A large British study, with almost 3000 patients, identified diabetes as main risk factor for delayed and nonunion fracture healing, the treatment of which causes large costs for the health system. In the past years, much progress has been made to treat common complications in diabetics. However, there is still a lack of advanced strategies to treat diabetic bone diseases. To develop such therapeutic strategies, mechanisms leading to massive bone alterations in diabetics have to be well understood. We herein describe an in vitro model displaying bone metabolism frequently observed in diabetics. The model is based on osteoblastic SaOS-2 cells, which in direct coculture, stimulate THP-1 cells to form osteoclasts. While in conventional 2D cocultures formation of mineralized matrix is decreased under pre-/diabetic conditions, formation of mineralized matrix is increased in 3D cocultures. Furthermore, we demonstrate a matrix stability of the 3D carrier that is decreased under pre-/diabetic conditions, resembling the in vivo situation in type 2 diabetics. In summary, our results show that a 3D environment is required in this in vitro model to mimic alterations in bone metabolism characteristic for pre-/diabetes. The ability to measure both osteoblast and osteoclast function, and their effect on mineralization and stability of the 3D carrier offers the possibility to use this model also for other purposes, e.g., drug screenings.

Type 2 diabetes-related proteins derived from an in vitro model of inflamed fat tissue

2018 ◽  
Vol 644 ◽  
pp. 81-92 ◽  
Author(s):  
Jean Paul ten Klooster ◽  
Alexandros Sotiriou ◽  
Sjef Boeren ◽  
Stefan Vaessen ◽  
Jacques Vervoort ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
In Vitro Model ◽  
Fat Tissue ◽  
Vitro Model ◽  
Related Proteins

Factors circulating in the blood of type 2 diabetes mellitus patients affect osteoblast maturation – Description of a novel in vitro model

2015 ◽  
Vol 332 (2) ◽  
pp. 247-258 ◽  
Author(s):  
Sabrina Ehnert ◽  
Thomas Freude ◽  
Christoph Ihle ◽  
Larissa Mayer ◽  
Bianca Braun ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
In Vitro Model ◽  
Vitro Model ◽  
Osteoblast Maturation

Four new triterpenoids from Chisocheton paniculatus and their anti-inflammatory activities

2012 ◽  
Vol 90 (2) ◽  
pp. 199-204 ◽  
Author(s):  
Ming-Hua Yang ◽  
Jun-Song Wang ◽  
Jian-Guang Luo ◽  
Xiao-Bing Wang ◽  
Ling-Yi Kong
Keyword(s):  
Spectroscopic Data ◽  
In Vitro Model ◽  
2D Nmr ◽  
No Production ◽  
Moderate Activity ◽  
Inhibitory Effects ◽  
Raw264.7 Macrophages ◽  
Anti Inflammatory ◽  
Vitro Model

As part of our continuing effort to discover new potential anti-inflammatory agents, four new triterpenoids, chisopanins L-O (1–4), were isolated from the twigs of Chisocheton paniculatus. Their structures were established on the basis of detailed analysis of spectroscopic data, including IR, NMR (1D and 2D NMR), and HR-ESI-MS. Their inhibitory effects against nitric oxide (NO) production were evaluated in an in vitro model of lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Compounds 1, 3, and 4 were found to exhibit moderate activity with IC50 values less than 10 μmol/L.

Potent inhibitory effects of steroids in an in vitro model of angiogenesis

1996 ◽  
Vol 150 (3) ◽  
pp. 457-464 ◽  
Author(s):  
D C Jaggers ◽  
W P Collins ◽  
S R Milligan
Keyword(s):  
In Vitro Model ◽  
Ovarian Follicle ◽  
Collagen Gel ◽  
Rat Aorta ◽  
Significant Inhibition ◽  
Inhibitory Effects ◽  
High Concentrations ◽  
Vitro Model ◽  
Very High

Abstract The regulation of angiogenesis in the ovarian follicle and corpus luteum is unclear. Steroids are produced at very high concentrations in these tissues and we therefore examined the effect of steroids on angiogenesis in vitro. Explants of rat aorta were embedded in collagen gel and cultured in serum-free medium. Capillary-like microvessels were produced from the explants and microvessel number and length were measured in the presence and absence of steroids. At a concentration of 10 μg/ml, cortisol, progesterone, 17α-hydroxyprogesterone and medroxyprogesterone acetate produced degeneration of microvessels after 7 days of steroid treatment (P<0·01). Androstenedione and tetrahydro-S-(11-deoxytetrahydro-cortisol) (tetrahydro S) produced degeneration at a slower rate: androstenedione inhibited microvessel growth after 11 days (P<0·01) and tetrahydro S after 14 days (P<0·05). Oestriol had no effect on microvessels; oestrone had a slow degenerative effect with significant inhibition seen after 14 days (P<0·01). Oestradiol-17β at a concentration of 10 μg/ml completely inhibited microvessel growth from the explant cultures (P<0·01) while at 1 μg/ml it caused degenerative effects on growing microvessels. The effects of oestradiol and cortisol were reversible on removal of steroid-containing medium and replacement with 10% serum. We conclude that oestradiol may modulate angiogenesis in tissues in which the steroid concentration is high. Journal of Endocrinology (1996) 150, 457–464

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