Faculty Opinions recommendation of Myotubes from lean and severely obese subjects with and without type 2 diabetes respond differently to an in vitro model of exercise.

2017 ◽  
Author(s):  
Joseph A Houmard
Keyword(s):  
Type 2 Diabetes ◽  
In Vitro Model ◽  
Severely Obese ◽  
Obese Subjects ◽  
Vitro Model

Myotubes from lean and severely obese subjects with and without type 2 diabetes respond differently to an in vitro model of exercise

2015 ◽  
Vol 308 (7) ◽  
pp. C548-C556 ◽  
Author(s):  
Yuan Z. Feng ◽  
Nataša Nikolić ◽  
Siril S. Bakke ◽  
Eili T. Kase ◽  
Kari Guderud ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Insulin Sensitivity ◽  
Mrna Expression ◽  
In Vitro Model ◽  
Oxidative Capacity ◽  
Severely Obese ◽  
Obese Subjects ◽  
Vitro Model

Exercise improves insulin sensitivity and oxidative capacity in skeletal muscles. However, the effect of exercise on substrate oxidation is less clear in obese and type 2 diabetic subjects than in lean subjects. We investigated glucose and lipid metabolism and gene expression after 48 h with low-frequency electrical pulse stimulation (EPS), as an in vitro model of exercise, in cultured myotubes established from lean nondiabetic subjects and severely obese subjects (BMI ≥ 40 kg/m2) with and without type 2 diabetes. EPS induced an increase in insulin sensitivity but did not improve lipid oxidation in myotubes from severely obese subjects. Thus, EPS-induced increases in insulin sensitivity and lipid oxidation were positively and negatively correlated to BMI of the subjects, respectively. EPS enhanced oxidative capacity of glucose in myotubes from all subjects. Furthermore, EPS reduced mRNA expression of slow fiber-type marker (MYH7) in myotubes from diabetic subjects; however, the protein expression of this marker was not significantly affected by EPS in either of the donor groups. On the contrary, mRNA levels of interleukin-6 (IL-6) and IL-8 were unaffected by EPS in myotubes from diabetic subjects, while IL-6 mRNA expression was increased in myotubes from nondiabetic subjects. EPS-stimulated mRNA expression levels of MYH7, IL-6, and IL-8 correlated negatively with subjects' HbA1c and/or fasting plasma glucose, suggesting an effect linked to the diabetic phenotype. Taken together, these data show that myotubes from different donor groups respond differently to EPS, suggesting that this effect may reflect the in vivo characteristics of the donor groups.

Type 2 diabetes-related proteins derived from an in vitro model of inflamed fat tissue

2018 ◽  
Vol 644 ◽  
pp. 81-92 ◽  
Author(s):  
Jean Paul ten Klooster ◽  
Alexandros Sotiriou ◽  
Sjef Boeren ◽  
Stefan Vaessen ◽  
Jacques Vervoort ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
In Vitro Model ◽  
Fat Tissue ◽  
Vitro Model ◽  
Related Proteins

Factors circulating in the blood of type 2 diabetes mellitus patients affect osteoblast maturation – Description of a novel in vitro model

2015 ◽  
Vol 332 (2) ◽  
pp. 247-258 ◽  
Author(s):  
Sabrina Ehnert ◽  
Thomas Freude ◽  
Christoph Ihle ◽  
Larissa Mayer ◽  
Bianca Braun ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
In Vitro Model ◽  
Vitro Model ◽  
Osteoblast Maturation

scholarly journals 3D Environment Is Required In Vitro to Demonstrate Altered Bone Metabolism Characteristic for Type 2 Diabetics

2021 ◽  
Vol 22 (6) ◽  
pp. 2925
Author(s):  
Victor Häussling ◽  
Romina H Aspera-Werz ◽  
Helen Rinderknecht ◽  
Fabian Springer ◽  
Christian Arnscheidt ◽  
...  
Keyword(s):  
Bone Metabolism ◽  
In Vitro Model ◽  
Bone Diseases ◽  
3D Environment ◽  
Type 2 Diabetics ◽  
Mineralized Matrix ◽  
Vitro Model

A large British study, with almost 3000 patients, identified diabetes as main risk factor for delayed and nonunion fracture healing, the treatment of which causes large costs for the health system. In the past years, much progress has been made to treat common complications in diabetics. However, there is still a lack of advanced strategies to treat diabetic bone diseases. To develop such therapeutic strategies, mechanisms leading to massive bone alterations in diabetics have to be well understood. We herein describe an in vitro model displaying bone metabolism frequently observed in diabetics. The model is based on osteoblastic SaOS-2 cells, which in direct coculture, stimulate THP-1 cells to form osteoclasts. While in conventional 2D cocultures formation of mineralized matrix is decreased under pre-/diabetic conditions, formation of mineralized matrix is increased in 3D cocultures. Furthermore, we demonstrate a matrix stability of the 3D carrier that is decreased under pre-/diabetic conditions, resembling the in vivo situation in type 2 diabetics. In summary, our results show that a 3D environment is required in this in vitro model to mimic alterations in bone metabolism characteristic for pre-/diabetes. The ability to measure both osteoblast and osteoclast function, and their effect on mineralization and stability of the 3D carrier offers the possibility to use this model also for other purposes, e.g., drug screenings.

Short-term Effects of Sleeve Gastrectomy on Type 2 Diabetes Mellitus in Severely Obese Subjects

2007 ◽  
Vol 17 (8) ◽  
pp. 1069-1074 ◽  
Author(s):  
J. Vidal ◽  
A. Ibarzabal ◽  
J. Nicolau ◽  
M. Vidov ◽  
S. Delgado ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Sleeve Gastrectomy ◽  
Short Term ◽  
Severely Obese ◽  
Obese Subjects ◽  
Short Term Effects

scholarly journals Mechanism of Action of Mangifera indica Leaves for Anti-Diabetic Activity

2019 ◽  
Vol 87 (2) ◽  
pp. 13 ◽  
Author(s):  
Dai-Hung Ngo ◽  
Dai-Nghiep Ngo ◽  
Thi Thanh Nhan Vo ◽  
Thanh Sang Vo
Keyword(s):  
Sulfonic Acid ◽  
In Vitro Model ◽  
Amylase Activity ◽  
Mangifera Indica ◽  
Significant Inhibition ◽  
No Production ◽  
Vitro Model ◽  
Cytotoxicity Effects

Diabetes is a major metabolic disorder whose prevalence is increasing daily. Medicinal plants have played an important role in the prevention and treatment of type 2 diabetes via prophylactic and therapeutic management. In this study, Mangifera Indica leaf (MIL) extract was investigated for its promising anti-diabetic activity via an in vitro model. It was found that MIL extract possessed significant inhibition on alpha-amylase activity up to (51.4 ± 2.7)% at a concentration of 200 µg/mL. Moreover, glucose adsorption capacity of MIL was identified at (2.7 ± 0.19) mM glucose/g extract. Furthermore, the extract caused a significant increase in glucose uptake up to (143 ± 9.3)% in LO-2 liver cells. Notably, MIL extract was effective in scavenging (63.3 ± 2.1)% 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and (71.6 ± 4.3)% 2,2-azinobis-3-ethyl benzothiazoline-6-sulfonic acid (ABTS)+ radicals and inhibiting (66 ± 4.9)% NO production from RAW264.7 cells without any cytotoxicity effects. Accordingly, M. indica leaves are suggested as a promising material for development of hypoglycemic products.

A Model for Predicting the Resolution of Type 2 Diabetes in Severely Obese Subjects Following Roux-en Y Gastric Bypass Surgery

2011 ◽  
Vol 21 (7) ◽  
pp. 910-916 ◽  
Author(s):  
Mark Thomas Hayes ◽  
Lynette Anne Hunt ◽  
Jonathan Foo ◽  
Yulia Tychinskaya ◽  
Richard Strawson Stubbs
Keyword(s):  
Type 2 Diabetes ◽  
Gastric Bypass ◽  
Bypass Surgery ◽  
Gastric Bypass Surgery ◽  
Severely Obese ◽  
Obese Subjects

Type 2 Diabetes is Associated with Lower Cardiorespiratory Fitness Independent of Pulmonary Function in Severe Obesity

2017 ◽  
Vol 125 (05) ◽  
pp. 301-306 ◽  
Author(s):  
Britta Wilms ◽  
Barbara Ernst ◽  
Martin Thurnheer ◽  
Christina Spengler ◽  
Bernd Schultes
Keyword(s):  
Type 2 Diabetes ◽  
Pulmonary Function ◽  
Cardiorespiratory Fitness ◽  
Severe Obesity ◽  
Forced Expiratory Volume ◽  
Maximal Heart Rate ◽  
Cross Sectional ◽  
Severely Obese ◽  
Obese Subjects

Abstract Both severe obesity and type 2 diabetes (T2DM) are associated with reduced pulmonary function and reduced cardiorespiratory fitness. We investigated whether T2DM further aggravates the impaired pulmonary function and cardiorespiratory fitness in subjects with severe obesity. In this cross-sectional study pulmonary function (forced expiratory volume within 1 s, FEV1; vital capacity, VC) was assessed in 65 severely obese subjects with T2DM (T2DM group) and 65 severely obese subjects without T2DM (non-T2DM group), pairwise matched for sex, age, weight, and height. In 30 of the matched pairs, cardiorespiratory fitness was assessed by an incremental bicycle test (peak workload, Wpeak, oxygen uptake, V̇O2,peak). FEV1 and VC did not differ between the T2DM and non-T2DM group (all p≥0.110), whereas Wpeak and V̇O2,peak - absolute values as well as relative to body mass - were significantly lower in subjects with T2DM compared to those without T2DM (all p≤0.030). Objective markers of maximal exertion, i. e., maximal heart rate and respiratory exchange ratio, did not differ between the 2 groups (both p≥0.245). The presence of T2DM in subjects with severe obesity is associated with lower cardiorespiratory fitness but with no further reduction in pulmonary function compared to subjects with severe obesity but without T2DM. While the cause-effect relationship of this association is not yet clear, these findings highlight the interplay between cardiorespiratory fitness and metabolic health, even in subjects with severe obesity.

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