scholarly journals Comparison of Neutral Compound Extraction from Archaeological Residues in Pottery Using Two Methodologies: A Preliminary Study

Separations ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 6
Author(s):  
Eleanora A. Reber
Keyword(s):  
Fatty Acid ◽  
Solvent Extraction ◽  
Acid Extraction ◽  
Methanol Extraction ◽  
Neutral Compound ◽  
Neutral Compounds ◽  
Preliminary Study ◽  
Chloroform Methanol

This study compares chloroform/methanol extraction and acidic methanol extraction of neutral compounds in absorbed lipid pottery residues from fourteen archaeological sherds. Previous studies have established that fatty acid extraction is more effective with acidic methanol extraction. This study suggests that acidic methanol extraction of neutral compounds, including sterols, alkanols, alkanes, and terpenoids, is more effective than or comparable to chloroform/methanol solvent extraction in most cases. The acidic methanol method extracts sterols, terpenoids, and alkanes more effectively than or comparably to chloroform/methanol extraction.

Preliminary Study of Lipid Content and Fatty Acid Composition in the Spottail Pinfish, Diplodus holbrooki

Copeia ◽  
1999 ◽  
Vol 1999 (3) ◽  
pp. 766 ◽  
Author(s):  
Ileana E. Clavijo ◽  
Carolyn S. Dunn ◽  
Pamela J. Seaton
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Lipid Content ◽  
Preliminary Study

A Compact Extraction Apparatus for Use with the Semimicro Method for Determining Total Lipids in Fish Meal

1971 ◽  
Vol 54 (5) ◽  
pp. 1132-1134
Author(s):  
Harry Miller ◽  
George M Knobl
Keyword(s):  
Extraction Method ◽  
Fish Meal ◽  
Total Lipids ◽  
Methanol Extraction ◽  
Extraction Apparatus ◽  
Chloroform Methanol

Abstract A compact glass extraction apparatus has been designed for use with the semimicro chloroform-methanol extraction method for determining lipids in fish meal. No further handling is required after placing the sample in the extractor; this eliminates manipulative errors and makes the procedure more efficient. Results obtained with the use of this extractor agreed favorably with those from the semimicro method in which a blender was used and with results from AOAC 7.052. It is recommended that the method be studied collaboratively.

scholarly journals Effect of fishing season on the fatty acid profile of Baltic herring

2020 ◽  
Vol 79 (OCE2) ◽  
Author(s):  
Janusz Pomianowski ◽  
Tomasz Żmijewski ◽  
Adam Wiżk
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fatty Acid Profile ◽  
Unsaturated Fatty Acids ◽  
Clupea Harengus ◽  
Acid Mixture ◽  
Experimental Sample ◽  
Fishing Season ◽  
Baltic Herring ◽  
Chloroform Methanol

AbstractMarine fish are a good source of unsaturated fatty acids, high nutritional value protein, vitamins and minerals. Baltic herring is one of the most frequently caught fish in Poland(1). It was demonstrated that the fishing season has an effect on the content of individual fatty acid fractions(2).The aim of the study was to analyse the fatty acid profile of Baltic herring flaps originating from two fishing seasons.The study material were flaps of Baltic herring (Clupea harengus) originating from two fishing seasons (April-June following spawning and October-December prior to spawning). For each fishing season, six batches of raw material were examined (n = 6). From each batch, flaps were taken from ten fish. The flaps were comminuted and thoroughly mixed to prepare an experimental sample from which adequate weights of analytical samples were taken. The quantitative and qualitative analysis of the fatty acid composition was conducted following the cold muscle lipid extraction with a mixture of chloroform : methanol (2:1). Fatty acid methylation was carried out with a chloroform : methanol : sulphuric acid mixture (100:100:1). The separation of fatty acids was performed by gas chromatography. A one-factor variance analysis method with Duncan's test (P ≤ 0.05) was used to evaluate the variation of mean values.The fishing season does not significantly affect the content of saturated and unsaturated fatty acids. Differences were found during the analysis of the individual groups of unsaturated acids. The amount of monounsaturated fatty acids in herring before spawning (25.27%) was significantly lower than that after spawning (35.91%) (P ≤ 0.05). The content of polyunsaturated fatty acids before spawning (43.99%) was significantly higher than that after spawning (33.49%) (P ≤ 0.05). Most changes in the fatty acid profile occur in the highly unsaturated fatty acids (HUFA). The most important change due to the health-promoting properties for humans is an increase in the amount of EPA from 6.24% to 9.84% after and before spawning, respectively. Analogous changes were demonstrated for DHA, whose amount increased from 9.64% to 24.56%. The obtained results demonstrate a more favourable fatty acid profile in herring caught before spawning.

Variations in the fatty acid compositions of the liver and gonad tissue of spiny eel (Mastacembelus mastacembelus) from Atatürk Dam Lake

2017 ◽  
Vol 42 (6) ◽  
Author(s):  
Semra Kaçar ◽  
Mehmet Başhan
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Liver Tissue ◽  
Methyl Esters ◽  
Saturated Fatty Acids ◽  
Liver Lipid ◽  
Docosapentaenoic Acid ◽  
Atatürk Dam Lake ◽  
Chloroform Methanol ◽  
Fatty Acid Compositions

AbstractObjective:The aim of the study was to investigate quantitative and qualitative fatty acid profiles of the gonad and liver tissue of femaleMethods:Total lipids were extracted with 5 mL of chloroform-methanol (2:1 v/v). Samples containing gonad and liver lipid were transesterified with acidified methanol. The fatty acid methyl esters were extracted with hexane. Fatty acids were detected by gas chromatography (GC).Results:The major saturated fatty acids (SFAs) were myristic acid (C14:0), palmitic acid (C16:0) and stearic acid (C18:0) in both gonad and liver tissue. Oleic acid (C18:1 ω-9) and palmitoleic acid (C16:1 ω-7) were the prominent monounsaturated fatty acid (MUFA). The dominant polyunsaturated fatty acids (PUFAs) were linoleic acid (LA, C18:2 ω-6), arachidonic acid (AA, C20:4 ω-6), docosapentaenoic acid (C22:5 ω-3) and docoesahexaenoic acid (DHA, C22:6 ω-3). The ratio of ω-3/ω-6 ranged from 1.55 to 3.44 and 1.18 to 2.71 in the gonad and liver tissue, respectively.Conclusion:The findings of this study will be useful for understanding the seasonal distribution of fatty acid composition in the liver and gonad of spiny eel.

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