Influence of Relative Humidity on Germination and Metal Accumulation in Vigna radiata Exposed to Metal-based Nanoparticles

The effect of single (0.1, 1, and 10 mg L−1) and binary mixtures (0.1 + 0.1, 1 + 1, and 10 + 10 mg L−1) of metal-based nanoparticles (CdO and CuO) on the germination of Vigna radiata was studied under two humidity ranges (70% and 80%). Filter paper-based tests were conducted. The surface-sterilized seeds were exposed to CdO and CuO under controlled environmental conditions (70% and 80% humidity at 35 °C). Germination rates were scored after 24 h and 48 h. The accumulation of metals was tested in seedlings after 48 h using inductively coupled plasma mass spectrometry. Compared with 70% humidity, the germination rate was higher under 80% humidity in all tested conditions. The germination rate of the CdO + CuO treatment was less than that of the single metal exposure under both humidities (70% and 80%) at 48 h. By two-way analysis of variance (ANOVA), we found that germination was greatly influenced by humidity. The accumulation of metal was higher in the CuO test than in the CdO test. Metal accumulation was concentration and humidity dependent, except for Cd accumulation in the CdO + CuO treatment. Here we show that the germination of seeds depends on the humidity and concentration of metal oxide nanoparticles. Understanding these strategies in seeds might help to avoid environmental and chemical stress and improve crop yield.

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Spatial Heterogeneity of Cadmium Effects on Salvia sclarea Leaves Revealed by Chlorophyll Fluorescence Imaging Analysis and Laser Ablation Inductively Coupled Plasma Mass Spectrometry

Materials ◽

10.3390/ma12182953 ◽

2019 ◽

Vol 12 (18) ◽

pp. 2953 ◽

Cited By ~ 8

Author(s):

Michael Moustakas ◽

Anetta Hanć ◽

Anelia Dobrikova ◽

Ilektra Sperdouli ◽

Ioannis-Dimosthenis S. Adamakis ◽

...

Keyword(s):

Mass Spectrometry ◽

Chlorophyll Fluorescence ◽

Quantum Yield ◽

Inductively Coupled Plasma ◽

Imaging Analysis ◽

Cd Accumulation ◽

Inductively Coupled ◽

Salvia Sclarea ◽

Icp Ms ◽

Plasma Mass Spectrometry

In this study, for a first time (according to our knowledge), we couple the methodologies of chlorophyll fluorescence imaging analysis (CF-IA) and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), in order to investigate the effects of cadmium (Cd) accumulation on photosystem II (PSII) photochemistry. We used as plant material Salvia sclarea that grew hydroponically with or without (control) 100 μM Cd for five days. The spatial heterogeneity of a decreased effective quantum yield of electron transport (ΦPSΙΙ) that was observed after exposure to Cd was linked to the spatial pattern of high Cd accumulation. However, the high increase of non-photochemical quenching (NPQ), at the leaf part with the high Cd accumulation, resulted in the decrease of the quantum yield of non-regulated energy loss (ΦNO) even more than that of control leaves. Thus, S. sclarea leaves exposed to 100 μM Cd exhibited lower reactive oxygen species (ROS) production as singlet oxygen (1O2). In addition, the increased photoprotective heat dissipation (NPQ) in the whole leaf under Cd exposure was sufficient enough to retain the same fraction of open reaction centers (qp) with control leaves. Our results demonstrated that CF-IA and LA-ICP-MS could be successfully combined to monitor heavy metal effects and plant tolerance mechanisms.

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Investigation of heavy-metal accumulation in selected plant samples using laser induced breakdown spectroscopy and laser ablation inductively coupled plasma mass spectrometry

Applied Physics A ◽

10.1007/s00339-008-4747-0 ◽

2008 ◽

Vol 93 (4) ◽

pp. 917-922 ◽

Cited By ~ 58

Author(s):

M. Galiová ◽

J. Kaiser ◽

K. Novotný ◽

J. Novotný ◽

T. Vaculovič ◽

...

Keyword(s):

Mass Spectrometry ◽

Heavy Metal ◽

Inductively Coupled Plasma ◽

Metal Accumulation ◽

Heavy Metal Accumulation ◽

Laser Induced Breakdown Spectroscopy ◽

Inductively Coupled ◽

Breakdown Spectroscopy ◽

Laser Induced Breakdown ◽

Plasma Mass Spectrometry

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Early-life metal exposure and schizophrenia: A proof-of-concept study using novel tooth-matrix biomarkers

European Psychiatry ◽

10.1016/j.eurpsy.2016.03.006 ◽

2016 ◽

Vol 36 ◽

pp. 1-6 ◽

Cited By ~ 21

Author(s):

A. Modabbernia ◽

E. Velthorst ◽

C. Gennings ◽

L. De Haan ◽

C. Austin ◽

...

Keyword(s):

Early Life ◽

Inductively Coupled Plasma ◽

Metal Exposure ◽

Proof Of Concept ◽

Psychotic Experiences ◽

Long Term Effects ◽

Concept Study ◽

Inductively Coupled ◽

Plasma Mass Spectrometry

AbstractBackgroundDespite evidence for the effects of metals on neurodevelopment, the long-term effects on mental health remain unclear due to methodological limitations. Our objective was to determine the feasibility of studying metal exposure during critical neurodevelopmental periods and to explore the association between early-life metal exposure and adult schizophrenia.MethodsWe analyzed childhood-shed teeth from nine individuals with schizophrenia and five healthy controls. We investigated the association between exposure to lead (Pb2+), manganese (Mn2+), cadmium (Cd2+), copper (Cu2+), magnesium (Mg2+), and zinc (Zn2+), and schizophrenia, psychotic experiences, and intelligence quotient (IQ). We reconstructed the dose and timing of early-life metal exposures using laser ablation inductively coupled plasma mass spectrometry.ResultsWe found higher early-life Pb2+ exposure among patients with schizophrenia than controls. The differences in log Mn2+ and log Cu2+ changed relatively linearly over time to postnatal negative values. There was a positive correlation between early-life Pb2+ levels and psychotic experiences in adulthood. Moreover, we found a negative correlation between Pb2+ levels and adult IQ.ConclusionsIn our proof-of-concept study, using tooth-matrix biomarker that provides direct measurement of exposure in the fetus and newborn, we provide support for the role of metal exposure during critical neurodevelopmental periods in psychosis.

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Cation and Zn Accumulation in Brown Seeds of the Euhalophyte Suaeda salsa Improves Germination Under Saline Conditions

Frontiers in Plant Science ◽

10.3389/fpls.2020.602427 ◽

2020 ◽

Vol 11 ◽

Author(s):

Jianrong Guo ◽

Lili Liu ◽

Ming Du ◽

Huaying Tian ◽

Baoshan Wang

Keyword(s):

Salt Tolerance ◽

Inductively Coupled Plasma ◽

Germination Rate ◽

High Salt ◽

Suaeda Salsa ◽

Inductively Coupled ◽

Plasma Mass Spectrometry ◽

Ion Contents ◽

Black Seeds

Salinity inhibits plant growth due to salt ion accumulation in plant cells and reduced absorption of other nutrients such as metal ions; however halophyte plants have evolved mechanisms to survive and thrive in high-salt conditions. The euhalophyte Suaeda salsa generates dimorphic seeds (black and brown), which show marked differences in germination and seedling growth under high-salt conditions. However, it is unclear whether their ionic status differs. Here, to provide insight on the role of ions in salt tolerance, we used inductively coupled plasma mass spectrometry to measure the ion contents in the dimorphic seeds from S. salsa plants treated with or without NaCl. We measured the macroelements Na, K, Mg, and Ca, and the microelements Mn, Fe, Zn, Cu, and Mo. NaCl-treated S. salsa plants produced seeds with significantly reduced metallic element contents and significantly increased Na+ contents. The brown seeds of S. salsa plants treated with 0 and 200 mM NaCl had much higher contents of K+, Ca2+, and Fe2+ compared with the black seeds. However, the S. salsa seeds (both black and brown) from NaCl-treated plants were significantly larger, and had higher germination rate and higher seedling salt tolerance compared with seeds from plants not treated with NaCl. Interestingly, we measured significantly higher Zn2+ contents in the brown seeds from plants treated with NaCl compared with the black seeds. This suggests that the high contents of Zn2+ and other cations affected seed development and salt tolerance during germination under high-salt conditions. These observations provide insight into the mechanisms of salt tolerance in this halophyte and inform efforts to increase salt tolerance in salt-sensitive species.

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Application of Inductively Coupled Plasma Mass Spectrometry Multielement Analysis in Fingernail and Toenail as a Biomarker of Metal Exposure

Journal of Analytical Toxicology ◽

10.1093/jat/33.2.92 ◽

2009 ◽

Vol 33 (2) ◽

pp. 92-98 ◽

Cited By ~ 55

Author(s):

J. P. Goulle ◽

E. Saussereau ◽

L. Mahieu ◽

D. Bouige ◽

S. Groenwont ◽

...

Keyword(s):

Mass Spectrometry ◽

Inductively Coupled Plasma ◽

Multielement Analysis ◽

Metal Exposure ◽

Inductively Coupled ◽

Plasma Mass Spectrometry

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The Utility of Spot vs 24-Hour Urine Samples for Metal Determination in Veterans With Retained Fragments

American Journal of Clinical Pathology ◽

10.1093/ajcp/aqaa144 ◽

2020 ◽

Author(s):

Joanna M Gaitens ◽

Clayton H Brown ◽

Frederick G Strathmann ◽

Hanna Xu ◽

Michael R Lewin-Smith ◽

...

Keyword(s):

Urine Sample ◽

Inductively Coupled Plasma ◽

Urine Samples ◽

Metal Exposure ◽

Good Reliability ◽

Spot Urine ◽

Inductively Coupled ◽

Spot Urine Sample ◽

Plasma Mass Spectrometry ◽

Good Agreement

Abstract Objectives The objective of this investigation is to explore the utility of using a spot urine sample in lieu of a 24-hour collection in assessing fragment-related metal exposure in war-injured veterans. Methods Twenty-four veterans collected each urine void over a 24-hour period in separate containers. Concentrations of 13 metals were measured in each void and in a pooled 24-hour sample using inductively coupled plasma mass spectrometry. To assess the reliability of spot sample measures over time, intraclass correlations (ICCs) were calculated across all spot samples. Lin’s concordance correlation coefficient was used to assess agreement between a randomly selected spot urine sample and each corresponding 24-hour sample. Results In total, 149 spot urine samples were collected. Ten of the 13 metals measured had ICCs more than 0.4, suggesting “fair to good” reliability. Concordance coefficients were more than 0.4 for all metals, suggesting “moderate” agreement between spot and 24-hour concentrations, and more than 0.6 for seven of the 13 metals, suggesting “good” agreement. Conclusions Our fair to good reliability findings, for most metals investigated, and moderate to good agreement findings for all metals, across the range of concentrations observed here, suggest the utility of spot urine samples to obtain valid estimates of exposure in the longitudinal surveillance of metal-exposed populations.

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Heavy metal accumulation in Lacistorhynchus dollfusi (Trypanorhyncha: Lacistorhynchidae) infecting Citharichthys sordidus (Pleuronectiformes: Bothidae) from Santa Monica Bay, Southern California

Parasitology ◽

10.1017/s003118201600038x ◽

2016 ◽

Vol 143 (6) ◽

pp. 794-799 ◽

Cited By ~ 6

Author(s):

C. COURTNEY-HOGUE

Keyword(s):

Heavy Metal ◽

Inductively Coupled Plasma ◽

Metal Accumulation ◽

Heavy Metal Accumulation ◽

Fish Host ◽

Inductively Coupled ◽

Santa Monica Bay ◽

Metal Levels ◽

Santa Monica ◽

Plasma Mass Spectrometry

SUMMARYThe accumulation of heavy metals in macroparasites of fish has been widely studied in freshwater environments. Less is known about metal uptake in cestodes parasitizing marine fish. Lacistorhynchus dollfusi is a common larval cestode parasite of Pacific sanddab (Citharichthys sordidus), a flatfish species inhabiting Santa Monica Bay. The ability of this cestode to concentrate metals in its tissues was compared with metal levels in its sanddab host. Fish and cestode tissue were analysed for 14 elements using Inductively Coupled Plasma Mass Spectrometry. The elements analysed were silver (Ag), arsenic (As), cadmium (Cd), chromium (Cr), copper (Cu), iron (Fe), mercury (Hg), potassium (K), lead (Pb), rubidium (Rb), selenium (Se), strontium (Sr), titanium (Ti) and zinc (Zn). Three of the 14 metals (Cu, Hg and Zn) were significantly greater in concentration in L. dollfusi compared with their levels in the liver, intestine and muscle of their fish host. They ranked in concentration from highest to lowest as follows: Zn > Cu > Hg. The ability of the cestode L. dollfusi to uptake metals at higher concentrations than its host warrants its consideration as a candidate for a heavy metal accumulation indicator of pollution exposure in Pacific sanddab.

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Simultaneous monitoring of cerebral metal accumulation in an experimental model of Wilson’s disease by laser ablation inductively coupled plasma mass spectrometry

BMC Neuroscience ◽

10.1186/1471-2202-15-98 ◽

2014 ◽

Vol 15 (1) ◽

pp. 98 ◽

Cited By ~ 22

Author(s):

Sorina Boaru ◽

Uta Merle ◽

Ricarda Uerlings ◽

Astrid Zimmermann ◽

Sabine Weiskirchen ◽

...

Keyword(s):

Mass Spectrometry ◽

Laser Ablation ◽

Experimental Model ◽

Inductively Coupled Plasma ◽

Wilson’S Disease ◽

Metal Accumulation ◽

Wilson's Disease ◽

Inductively Coupled ◽

Plasma Mass Spectrometry

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Occupational Exposure to Metals and Serum Adiponectin Levels Among Shipyard Workers: A Cohort Study with Weighted Quantile Sum Regression Analyses

10.21203/rs.3.rs-1150249/v1 ◽

2021 ◽

Author(s):

Chen-Jung Wu ◽

A-Chuan Ho ◽

Shih-Ya Chen ◽

Chih-Hong Pan ◽

Hsiao-Chi Chuang ◽

...

Keyword(s):

Repeated Measures ◽

Inductively Coupled Plasma ◽

Joint Effect ◽

Serum Adiponectin ◽

Metal Exposure ◽

Enzyme Linked Immunosorbent Assay ◽

Inductively Coupled ◽

Increased Risk ◽

Metal Levels ◽

Plasma Mass Spectrometry

Abstract Background: The influence of welding-associated air pollutants on workers’ health is mainly regarded as a core issue in public health and occupational medicine. Previous studies have indicated that workers exposed to metal fumes had an increased risk of metabolic syndrome, which was correlated with decreased serum adiponectin levels. This study aimed to explore whether heavy metal exposure affects the concentration of adiponectin among welding workers. Methods: The study participants were recruited from a shipyard with 31 office workers and 100 welding workers in 2015. Urinary metal concentrations were measured by inductively coupled plasma–mass spectrometry. Serum adiponectin was measured by enzyme-linked immunosorbent assay. Inferential statistics on repeated measures were performed using generalized estimating equations (GEEs). A weighted quantile sum (WQS) regression model was conducted to examine the joint effect of the association of multimetal exposure with serum adiponectin. Results: After adjustment for all confounding variables through a GEE analysis, significantly negative associations of numerous urinary metals with serum adiponectin were detected in the welding workers, including Cr (β = -0.088; 95% CI: -0.148, -0.027), Mn (β = -0.174; 95% CI: -0.267, -0.081), Co (β = -0.094; 95% CI: -0.158, -0.029), Ni (β = -0.108; 95% CI: -0.208, -0.008), Cd (β = -0.067; 95% CI: -0.115, -0.018), and Pb (β = -0.089; 95% CI: -0.163, -0.015). The contributions of multiple urinary metal levels to serum adiponectin levels, determined individually by WQS regression, suggested that Pb was the greatest contributor. Conclusions: Welding workers exposed to heavy metals such as Pb, Cr, Co, Mn, Ni, and Cd might have reduced serum adiponectin levels and an increased risk of cardiovascular disease.

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