Exposure to Zearalenone Leads to Metabolic Disruption and Changes in Circulating Adipokines Concentrations in Pigs

Zearalenone (ZEN) is a mycotoxin classified as an endocrine disruptor. Many endocrine disruptors are also metabolic disruptors able to modulate energy balance and inflammatory processes in a process often involving a family of protein hormones known as adipokines. The aim of our study was to elucidate the role of ZEN as metabolic disruptor in pigs by investigating the changes in energy balance and adipokines levels in response to different treatment diets. To this end, weaned piglets (n = 10/group) were exposed to either basal feed or feed contaminated with 680 and 1620 µg/kg ZEN for 28 days. Serum samples collected at days 7 and 21 were subjected to biochemistry analysis, followed by determination of adipokine levels using a combined approach of protein array and ELISA. Results indicate that ZEN has an impact on lipid and glucose metabolism that was different depending on the dose and time of exposure. In agreement with these changes, ZEN altered circulating adipokines concentrations, inducing significant changes in adiponectin, resistin, and fetuin B. Our results suggest that ZEN may function as a natural metabolism-disrupting chemical.

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Serum YKL-40 Levels in Patients with Gastric Cancer

Biomarkers in Cancer ◽

10.4137/bic.s7154 ◽

2011 ◽

Vol 3 ◽

pp. BIC.S7154 ◽

Cited By ~ 7

Author(s):

Veyis Itik ◽

Ozgur Kemik ◽

Ahu Kemik ◽

A. Cumhur Dulger ◽

Aziz Sümer ◽

...

Keyword(s):

Gastric Cancer ◽

Serum Levels ◽

Enzyme Linked Immunosorbent Assay ◽

Colorectal Cancers ◽

Healthy Population ◽

Serum Samples ◽

Future Studies ◽

Male Patients

Aims and background YKL-40 is secreted by several types of tumors. Increased serum YKL-40 levels have been reported in prostate, glioblastoma, breast and colorectal cancers. Determination of YKL-40 levels may serve as a valuable biomarker for the diagnosis and treatment of gastric cancer. The purpose of this study was to determine the serum YKL-40 levels expressed in gastric carcinomas. Methods Between 2009 and 2011, we retrospectively reviewed 100 patients with gastric cancer and compared their serum samples to 75 healthy volunteers. YKL-40 levels were determined by an enzyme-linked immunosorbent assay (ELISA). Results We found significantly higher serum levels of YKL-40 in patients with gastric cancer compared to the healthy population ( P < 0.0001). We also found significant differences in serum YKL-40 levels between female and male patients with gastric cancer ( P < 0.01). Conclusions YKL-40 is over-expressed in gastric cancer, suggesting a more aggressive phenotype. YKL-40 may be a useful serum biomarker for gastric cancer identification, and future studies should focus on the role of YKL-40 in the tumorigenesis of gastric cancer and responsiveness toward treatment.

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Olfactory Dysfunction: A Complication of Diabetes or a Factor That Complicates Glucose Metabolism? A Narrative Review

Journal of Clinical Medicine ◽

10.3390/jcm10235637 ◽

2021 ◽

Vol 10 (23) ◽

pp. 5637

Author(s):

Evanthia Gouveri ◽

Nikolaos Papanas

Keyword(s):

Diabetes Mellitus ◽

Peripheral Neuropathy ◽

Energy Balance ◽

Glucose Metabolism ◽

Microvascular Complications ◽

Diabetic Complication ◽

Olfactory Dysfunction ◽

Narrative Review ◽

Central Neuropathy

The present narrative review presents emerging data regarding the association between diabetes mellitus and olfactory dysfunction and discusses the role of olfactory dysfunction in glucose metabolism. We searched relevant published articles in PubMed and Google Scholar until October 2021. Main key words included “olfactory dysfunction”, “diabetes mellitus”, and “glucose metabolism”. Olfactory dysfunction has been associated with diabetes mellitus. Furthermore, it has been proposed to be a diabetic complication, given that it has been linked with microvascular complications, such as diabetic peripheral neuropathy. Interestingly, it has been suggested that olfactory dysfunction is a manifestation of central neuropathy in diabetes, a hypothesis based on the observation that diabetes, olfactory dysfunction, and cognitive decline often coexist. However, evidence is limited and inconsistent. More importantly, olfactory and endocrine systems are closely linked, and olfactory dysfunction plays a significant role in glucose metabolism and obesity. Indeed, food behaviour and energy balance are influenced by olfaction status.

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Determination of Mammalian Lignans in Biological Samples by Modified Gas Chromatography/Mass Spectrometry

Journal of AOAC International ◽

10.1093/jaoac/90.3.641 ◽

2007 ◽

Vol 90 (3) ◽

pp. 641-646 ◽

Cited By ~ 5

Author(s):

Ajay Bommareddy ◽

Bhanu L Arasada ◽

Duane P Mathees ◽

Chandradhar Dwivedi

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Gas Chromatography Mass Spectrometry ◽

Serum Samples ◽

Selected Ion Monitoring ◽

Beneficial Effects ◽

Chromatography Mass Spectrometry ◽

Flaxseed Meal

Abstract Lignans in flaxseed have been part of the human diet for centuries. In 1955, the isolation and structure of the lignan derivative secoisolariciresinol diglucoside (SDG) was reported. The biological role of SDG and mammalian lignan metabolites enterodiol and enterolactone was initially reported 20 years later. Experimental evidences showed the beneficial effects of lignans on breast, colon, and thyroid cancer. A modified gas chromatography/mass spectrometry (GC/MS) assay was developed for lignans in serum and colon samples of rats fed flaxseed meal. The method developed for the analysis of metabolites involves extraction and derivatization of samples and quantitative analysis by selected ion monitoring using GC/MS. The levels of lignan metabolites enterodiol and enterolactone were determined to be 0.013 and 0.23 M in serum samples and 0.008 and 1.63 M in colon samples.

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Immunoneutralization and immunocytochemical localization of inhibin α subunit during the mid-luteal phase in the stump-tailed macaque

Journal of Endocrinology ◽

10.1677/joe.0.1330341 ◽

1992 ◽

Vol 133 (3) ◽

pp. 341-NP ◽

Cited By ~ 6

Author(s):

H. M. Fraser ◽

K. B. Smith ◽

S. F. Lunn ◽

G. M. Cowen ◽

K. Morris ◽

...

Keyword(s):

Corpus Luteum ◽

Luteal Phase ◽

Treatment Cycle ◽

Serum Concentrations ◽

Serum Samples ◽

Α Subunit ◽

Late Luteal Phase ◽

Early Follicular Phase

ABSTRACT The putative endocrine role of inhibin in the control of FSH secretion during the luteal phase in the primate was investigated by immunoneutralization. Antisera against the 1–23 amino acid sequence of the N-terminus of the human inhibin α subunit were raised in a ewe and three macaques. Antisera (10–20 ml) were administered to macaques on day 8/9 of the luteal phase and serum samples collected during the treatment cycle and post-treatment cycle for determination of FSH, oestradiol and progesterone. In addition, localization of inhibin within the macaque ovary at this stage of the luteal phase was investigated using the ovine antiserum. Intense immunostaining was localized within the granulosa-lutein cells of the corpus luteum with absence of staining in the thecalutein cells or other ovarian compartments. Administration of antisera was without significant effect on serum concentrations of FSH when compared with control animals, either during the first 24 h of detailed observation or for the following 10-day period of the late luteal phase and subsequent early follicular phase. These results provide further evidence that the corpus luteum is the major source of inhibin immunoreactivity during the primate menstrual cycle, but fail to support an endocrine role for inhibin in the suppression of FSH secretion. Journal of Endocrinology (1992) 133, 341–347

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The effects of oral steroid therapy on prolidase enzyme activity in patients with nasal polyps

Turkish Journal of Biochemistry ◽

10.1515/tjb-2019-0204 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Adnan Ekinci ◽

Huseyin Kayadibi ◽

Emre Demir ◽

Muge Ozcan

Keyword(s):

Enzyme Activity ◽

Steroid Therapy ◽

Nasal Polyps ◽

Oral Steroid ◽

Serum Samples ◽

Before And After ◽

Oral Steroids ◽

Inflammatory Processes ◽

Oral Steroid Therapy

Abstract Objective To compare prolidase enzyme activity (PEA) in serum and polyp specimens of patients with nasal polyps obtained before and after the oral steroid therapy. Materials and methods Thirty three patients with nasal polyps (39 ± 13 years) received 1 mg/kg of oral steroids. Serum samples were collected from each patient, but nasal polyp specimens could be obtained only from 23 patients (38 ± 13 years) before and after the oral steroid therapy. PEA was measured by ELISA method. Results Serum PEA values were 210 (176–242) U/L and 184 (147–217) U/L before and after the oral steroid therapy, respectively (p = 0.015). Polyp tissue PEA was 1337 (738–2130) U/g and 871 (590–1663) U/g before and after the oral steroid therapy, respectively (p = 0.429). Conclusion In patients with nasal polyps, significantly lower serum PEA after the oral steroid therapy may be a consequence of the role of prolidase enzyme in inflammatory processes which are important for the development of nasal polyps. More comprehensive studies with larger sample sizes are needed to elucidate the role of PEA in the pathogenesis of nasal polyps.

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THE ROLE OF ADRENOCORTICAL HORMONES IN THE REGULATION OF ORGANISM RESPONSE TO INTRATRACHEAL INJECTIONS OF ELECTROLYSIS DUST TO WISTAR RATS

Hygiene and Sanitation ◽

10.18821/0016-9900-2019-98-8-820-826 ◽

2019 ◽

Vol 98 (8) ◽

pp. 820-826

Author(s):

Ludmila V. Khripach ◽

T. D. Knyazeva ◽

E. V. Zheleznyak ◽

Z. I. Koganova ◽

M. A. Pinigin ◽

...

Keyword(s):

Wistar Rats ◽

Aluminum Production ◽

Human Organism ◽

Experimental Point ◽

Serum Samples ◽

Adrenocortical Hormones ◽

Cortisol Levels ◽

Glutamyl Transferase

Introduction. Adrenocortical hormones play a leading role in the adaptation of human organism to damaging factors. The aim of this study is to compare levels of cortisol and biochemical markers of organism damage (MOD) in blood samples of rats under a model of electrolysis dust (ED) inhalation exposure. ED being component of emissions in aluminum production and has high content of resinous substances (RS), including 3,4-benzpyrene. Material and methods. Male Wistar rats were injected ED intratracheally once per month, in doses corresponding to keeping of animals at ED concentrations of 1.0; 5.2 and 25.1 mg/m3, calculated on RS mass. 2 weeks after the 1st and 2nd ED administration and 6 days after the 3rd one, blood serum samples were used for determination of cortisol levels (ELISA) and the following 6 MOD: the intensity of luminol-enchanced chemiluminescence, activities of catalase, N-acetyl-β-D-glucosaminidase (NAG), acid DNAse, acetylesterase and γ-glutamyl transferase (GGT). Results. 2 weeks after the 1st and 2nd ED administrations (cortisol content 2 times reduced or not changed), similar significant changes were observed in almost all MOD; 2nd experimental point, if comparing with 1st point, had slight increase in oxidative stress and significant rise in GGT activity (a marker of precancer changes) at the maximum dose. On the contrary, 6 days after the 3rd injection of ED, in parallel with the increase of cortisol content in rat blood by 1.5 - 2 times, small significant changes were found for only one marker (NAG). The obtained data fit into existing concepts about protective role of cortisol and biphasic nature of its release into the blood, allow us to consider the absence of MOD, in parallel with rising levels of C, as a transit phase and have common problem aspects with CIRCI syndrome (Critical illness-Related Corticosteroid Insufficiency) in emergency medicine. Conclusion. Determination of cortisol levels by ELISA assay can be easily incorporated into any toxicological protocol as index of organism adaptive response, but further investigations are needed to clarify its characteristics and to build continuous model from separate time-dose points.

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The role of indicators of connective tissue metabolism in morpho-biochemical screening of purulent-inflammatory processes in cats

Veterinariya, Zootekhniya i Biotekhnologiya ◽

10.36871/vet.zoo.bio.202104002 ◽

2021 ◽

Vol 1 (4) ◽

pp. 16-22

Author(s):

D. A. Stuzhuk ◽

◽

V. I. Izdepsky ◽

P. A. Rudenko ◽

◽

...

Keyword(s):

Connective Tissue ◽

Blood Parameters ◽

Pathological Process ◽

Biochemical Screening ◽

Tissue Metabolism ◽

Inflammatory Processes ◽

Connective Tissue Metabolism ◽

Late Stages

The article presents data on the role of indicators of connective tissue metabolism in morphobiochemical screening of purulent-inflammatory processes in cats. It was found that the acute purulent-inflammatory process in cats proceeds with significant shifts in clinical, morphological and biochemical blood parameters. With inflammation in cats, the level of sialic acids, the content of hexoses associated with proteins and the redistribution of their fractions in the blood serum at the late stages of the pathological process increases in the direction of increasing the hexoses of glycoproteins. It is shown that the determination of indicators of connective tissue metabolism during morpho-biochemical screening of purulent-inflammatory processes of surgical profile in cats will improve the diagnosis, determination of the severity of the course, prediction of the outcome and existing methods of their treatment.

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Influence of Magnetic Fields on Solar Hydrodynamics: Experimental Results

International Astronomical Union Colloquium ◽

10.1017/s025292110005329x ◽

1977 ◽

Vol 36 ◽

pp. 143-180 ◽

Cited By ~ 10

Author(s):

J.O. Stenflo

Keyword(s):

Solar Activity ◽

Energy Balance ◽

Magnetic Fields ◽

Solar Atmosphere ◽

General Problem ◽

Solar Rotation ◽

Active Regions ◽

Physical Conditions ◽

Dynamic Phenomena

It is well-known that solar activity is basically caused by the Interaction of magnetic fields with convection and solar rotation, resulting in a great variety of dynamic phenomena, like flares, surges, sunspots, prominences, etc. Many conferences have been devoted to solar activity, including the role of magnetic fields. Similar attention has not been paid to the role of magnetic fields for the overall dynamics and energy balance of the solar atmosphere, related to the general problem of chromospheric and coronal heating. To penetrate this problem we have to focus our attention more on the physical conditions in the ‘quiet’ regions than on the conspicuous phenomena in active regions.

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A Molecular Approach to Immunoscintigraphy: A Study of the T-Antigen Conformation on the Surface of Tumors

Nuklearmedizin ◽

10.1055/s-0038-1624353 ◽

1987 ◽

Vol 26 (01) ◽

pp. 1-6 ◽

Cited By ~ 2

Author(s):

S. Selvaraj ◽

M. R. Suresh ◽

G. McLean ◽

D. Willans ◽

C. Turner ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Tumor Cell ◽

T Antigen ◽

Human Carcinomas ◽

Animal Tumor ◽

Synthetic Antigens

The role of glycoconjugates in tumor cell differentiation has been well documented. We have examined the expression of the two anomers of the Thomsen-Friedenreich antigen on the surface of human, canine and murine tumor cell membranes both in vitro and in vivo. This has been accomplished through the synthesis of the disaccharide terminal residues in both a and ß configuration. Both entities were used to generate murine monoclonal antibodies which recognized the carbohydrate determinants. The determination of fine specificities of these antibodies was effected by means of cellular uptake, immunohistopathology and immunoscintigraphy. Examination of pathological specimens of human and canine tumor tissue indicated that the expressed antigen was in the β configuration. More than 89% of all human carcinomas tested expressed the antigen in the above anomeric form. The combination of synthetic antigens and monoclonal antibodies raised specifically against them provide us with invaluable tools for the study of tumor marker expression in humans and their respective animal tumor models.

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On The Mechanism Of Heparin-Induced Potentiation Of Platelet Aggregation

10.1055/s-0038-1652598 ◽

1981 ◽

Author(s):

M Yamamoto ◽

K Watanabe ◽

Y Ando ◽

H Iri ◽

N Fujiyama ◽

...

Keyword(s):

Platelet Aggregation ◽

Platelet Activation ◽

Coagulation Factors ◽

Marked Enhancement ◽

Matrix Bound ◽

Induced Aggregation ◽

Aggregation Of Platelets ◽

Plasma Heparin

It has been suggested that heparin caused potentiation of aggregation induced by ADP or epinephrine. The exact mechanism of heparin-induced platelet activation, however, remained unknown. In this paper, we have investigated the role of anti-thrombin III ( AT ) in heparin-induced platelet activation using purified AT and AT depleted plasma. When ADP or epinephrine was added to citrated PRP one minute after addition of heparin ( 1 u/ml, porcine intestinal mucosal heparin, Sigma Co. USA ), marked enhancement of platelet aggregation was observed, compared with the degree of aggregation in the absence of heparin. However, in platelet suspensions prepared in modified Tyrode’s solution, heparin exhibited no potentiating effect on platelet aggregation induced by epinephrine or ADP. Potentiation of epinephrine- or ADP-induced platelet aggregation by heparin was demonstrated when purified AT was added to platelet suspensions at a concentration of 20 μg/ml. AT depleted plasma, which was prepared by immunosorption using matrix-bound antibodies to AT, retained no AT, while determination of α1-antitrypsinα2- macroglobulin and fibrinogen in AT depleted plasma produced values which corresponded to those of the original plasma when dilution factor was taken into account. The activities of coagulation factors were also comparable to those of the original plasma. Heparin exhibited potentiating effect on ADP- or epinephrine-induced aggregation of platelets in original plasma, but no effect in AT depleted plasma. When purified AT was added back to AT depleted plasma at a concentration of 20 μg/ml, potentiation of platelet aggregation by heparin was clearly demonstrated.Our results suggest that effect of heparin on platelet aggregation is also mediated by anti-thrombin III.

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