scholarly journals Whole Transcriptomic Analysis Provides Insights into Molecular Mechanisms for Toxin Biosynthesis in a Toxic Dinoflagellate Alexandrium catenella (ACHK-T)

Toxins ◽  
2017 ◽  
Vol 9 (7) ◽  
pp. 213 ◽  
Author(s):  
◽  
◽  
◽  
Keyword(s):  
Molecular Mechanisms ◽  
Transcriptomic Analysis ◽  
Toxic Dinoflagellate ◽  
Alexandrium Catenella

scholarly journals Distribution of Growth-Inhibiting Bacteria against the Toxic Dinoflagellate Alexandrium catenella (Group I) in Akkeshi-Ko Estuary and Akkeshi Bay, Hokkaido, Japan

2020 ◽  
Vol 11 (1) ◽  
pp. 172
Author(s):  
Yuka Onishi ◽  
Akihiro Tuji ◽  
Atsushi Yamaguchi ◽  
Ichiro Imai
Keyword(s):  
Surface Waters ◽  
Seagrass Bed ◽  
Bacterial Strains ◽  
Toxic Dinoflagellate ◽  
Sequence Analyses ◽  
Alexandrium Catenella ◽  
Group I ◽  
Colony Forming Units ◽  
Rrna Sequences ◽  
Growth Inhibiting

The distribution of growth-inhibiting bacteria (GIB) against the toxic dinoflagellate Alexandrium catenella (Group I) was investigated targeting seagrass leaves and surface waters at the seagrass bed of Akkeshi-ko Estuary and surface waters of nearshore and offshore points of Akkeshi Bay, Japan. Weekly samplings were conducted from April to June in 2011. GIBs were detected from surface of leaves of the seagrass Zostera marina in Akkeshi-ko Estuary (7.5 × 105–4.7 × 106 colony-forming units: CFU g−1 wet leaf) and seawater at the stations in Akkeshi Bay (6.7 × 100–1.1 × 103 CFU mL−1). Sequence analyses revealed that the same bacterial strains with the same 16S rRNA sequences were isolated from the surface biofilm of Z. marina and the seawater in the Akkeshi Bay. We therefore strongly suggested that seagrass beds are the source of algicidal and growth-inhibiting bacteria in coastal ecosystems. Cells of A.catenella were not detected from seawaters in Akkeshi-ko Estuary and the coastal point of Akkeshi Bay, but frequently detected at the offshore point of Akkeshi Bay. It is suggested that A.catenella populations were suppressed by abundant GIBs derived from the seagrass bed, leading to the less toxin contamination of bivalves in Akkeshi-ko Estuary.

scholarly journals Proteomic analysis of a toxic dinoflagellate Alexandrium catenella under different growth phases and conditions

2012 ◽  
Vol 57 (25) ◽  
pp. 3328-3341 ◽  
Author(s):  
DaZhi Wang ◽  
Lin Lin ◽  
MingHua Wang ◽  
Cheng Li ◽  
HuaSheng Hong
Keyword(s):  
Proteomic Analysis ◽  
Growth Phases ◽  
Toxic Dinoflagellate ◽  
Alexandrium Catenella

scholarly journals Accumulation and depuration profiles of PSP toxins in the short-necked clam Tapes japonica fed with the toxic dinoflagellate Alexandrium catenella

Toxicon ◽  
2006 ◽  
Vol 48 (3) ◽  
pp. 323-330 ◽  
Author(s):  
Mohamad Samsur ◽  
Yasunaga Yamaguchi ◽  
Takefumi Sagara ◽  
Tomohiro Takatani ◽  
Osamu Arakawa ◽  
...  
Keyword(s):  
Toxic Dinoflagellate ◽  
Alexandrium Catenella ◽  
Psp Toxins

Comparative transcriptomic analysis of flowering induction in pitaya induced by supplementary lighting

2019 ◽  
Author(s):  
Rui Xiong ◽  
Liu Chengli ◽  
Min Xu ◽  
Shuang-Shuang Wei ◽  
Hua Tang
Keyword(s):  
Time Course ◽  
Molecular Mechanisms ◽  
De Novo ◽  
Floral Induction ◽  
Transcriptomic Analysis ◽  
Pcr Analysis ◽  
Reverse Transcription Pcr ◽  
Transcription Factor Genes ◽  
Supplementary Lighting ◽  
Transcriptomic Level

Abstract Background Pitayas are currently attracting considerable interest as a fruit with many health benefits. However, the lack of natural light after November in Hainan, China, severely restricts the production of pitaya in winter. To further explore the molecular mechanisms regulating flowering in pitaya, we used de novo RNA sequencing-based transcriptomic analysis for four stages of pitaya subjected to light induction. Results We assembled 68113 unigenes in total, comprising 29782 unigenes with functional annotations in the NR database, 20716 annotations in SwissProt, 18088 annotations in KOG, and 11059 annotations in KEGG. Comparison between different samples revealed different numbers of significantly differentially expressed genes (DEGs). A number of DEGs involved in energy metabolism-related processes and plant hormones were detected. Moreover, we discovered many CONSTANS-LIKE, FLOWERING LOCUS T and other DEGs involved in direct regulation of flowering, along with CDF and TCP, which function as typical transcription factor genes in the flowering process. At the transcriptomic level, we confirmed 13 DEGs with different functions in the time-course response to light-induced flowering by quantitative reverse-transcription PCR analysis. Conclusions These DEGs may include some key genes that control the floral-induction network, increasing our understanding of the molecular mechanism of floral regulation in pitaya. These findings will also aid the development of biotechnologies aimed at creating a variant of pitaya that is less sensitive to light conditions and blooms throughout the year.

Transcriptomic analysis to elucidate the molecular mechanisms that underlie feed efficiency in meat-type chickens

2015 ◽  
Vol 290 (5) ◽  
pp. 1673-1682 ◽  
Author(s):  
Jeeyoung Lee ◽  
Arthur B. Karnuah ◽  
Romdhane Rekaya ◽  
Nicholas B. Anthony ◽  
Samuel E. Aggrey
Keyword(s):  
Feed Efficiency ◽  
Molecular Mechanisms ◽  
Transcriptomic Analysis ◽  
Meat Type

scholarly journals PSVII-19 Transcriptomic analysis of the liver of laying hens fed diets containing supplemental fat at early laying stages

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 353-353
Author(s):  
Gi Ppeum Han ◽  
Geun Hyeon Park ◽  
Jong Hyuk Kim ◽  
Hyeon Seok Choi ◽  
Hwan Ku Kang ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Molecular Mechanisms ◽  
Acid Metabolism ◽  
Laying Hens ◽  
Acid Synthesis ◽  
Transcriptomic Analysis ◽  
Cdna Libraries ◽  
Feeding Diets ◽  
Supplemental Fat ◽  
Liver Tissues

Abstract Feeding diets containing supplemental fat to laying hens is reported to ameliorate pathogenesis of fatty liver hemorrhagic syndrome (FLHS). However, molecular mechanisms for this positive effect have not been investigated. Thus, we conducted a transcriptomic analysis of the liver of laying hens fed diets containing supplemental fat at early laying stages. Two dietary treatments included basal diets with no supplemental fat and basal diets supplemented with 3.0% tallow. A total of 256 18-week-old Hy-line Brown laying hens were allotted to 1 of 2 treatments. Diets were fed to hens for 12 weeks. At the end of the experiment (30 weeks of age), 5 hens with similar BW per treatment were euthanized to collect liver tissues. The cDNA libraries were constructed with extracted RNA from the liver tissues, and sequenced using the Illumina Nextseq 500 sequencer. Genes with False Discovery Rate (FDR) < 0.05 were defined as differentially expressed genes (DEGs). Results indicated that a total of 951 DEGs were identified, with 483 being up-regulated and 468 being down-regulated in the liver of hens fed diets containing 3.0% tallow. The KEGG analysis revealed that the DEGs belong to several biological pathways such as cellular signaling pathways, carbon metabolism, glycolysis, gluconeogenesis, TCA cycle, amino acid metabolism, drug metabolism, and glycerophospholipid metabolism. Especially for fatty acid metabolism, the DEGs associated with fatty acid degradation (ECI2, ACSL1, HADHA, EHHADH, ACOX1, CPT1) were up-regulated, whereas those related to fatty acid synthesis (ACACA, ACSL5, FASN) were down-regulated in the liver of hens fed diets containing 3.0% tallow, which indicates that supplemental fat in diets may increase fat oxidation but decrease fat synthesis in the liver. These results provide the molecular insights for hepatic lipid metabolisms by feeding diets containing supplemental fat to laying hens at early laying stages.

scholarly journals Integrated Metabolomic and Transcriptomic Analysis to Characterize Cutin Biosynthesis between Low- and High-Cutin Genotypes of Capsicum chinense Jacq

2020 ◽  
Vol 21 (4) ◽  
pp. 1397 ◽  
Author(s):  
Purushothaman Natarajan ◽  
Tolulope Abodunrin Akinmoju ◽  
Padma Nimmakayala ◽  
Carlos Lopez-Ortiz ◽  
Marleny Garcia-Lozano ◽  
...  
Keyword(s):  
Abiotic Stresses ◽  
Biosynthetic Pathway ◽  
Molecular Mechanisms ◽  
Extreme Temperature ◽  
Transcriptome Profiling ◽  
Transcriptomic Analysis ◽  
Biotic And Abiotic Stresses ◽  
Habanero Pepper ◽  
And Storage ◽  
Gdsl Lipase

Habanero peppers constantly face biotic and abiotic stresses such as pathogen/pest infections, extreme temperature, drought and UV radiation. In addition, the fruit cutin lipid composition plays an important role in post-harvest water loss rates, which in turn causes shriveling and reduced fruit quality and storage. In this study, we integrated metabolome and transcriptome profiling pertaining to cutin in two habanero genotypes: PI 224448 and PI 257145. The fruits were selected by the waxy or glossy phenotype on their surfaces. Metabolomics analysis showed a significant variation in cutin composition, with about 6-fold higher cutin in PI 257145 than PI 224448. It also revealed that 10,16-dihydroxy hexadecanoic acid is the most abundant monomer in PI 257145. Transcriptomic analysis of high-cutin PI 257145 and low-cutin PI 224448 resulted in the identification of 2703 statistically significant differentially expressed genes, including 1693 genes upregulated and 1010 downregulated in high-cutin PI 257145. Genes and transcription factors such as GDSL lipase, glycerol-3 phosphate acyltransferase 6, long-chain acyltransferase 2, cytochrome P450 86A/77A, SHN1, ANL2 and HDG1 highly contributed to the high cutin content in PI 257145. We predicted a putative cutin biosynthetic pathway for habanero peppers based on deep transcriptome analysis. This is the first study of the transcriptome and metabolome pertaining to cutin in habanero peppers. These analyses improve our knowledge of the molecular mechanisms regulating the accumulation of cutin in habanero pepper fruits. These resources can be built on for developing cultivars with high cutin content that show resistance to biotic and abiotic stresses with superior postharvest appearance.

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