scholarly journals Combining a Simple Method for DNA/RNA/Protein Co-Purification and Arabidopsis Protoplast Assay to Facilitate Viroid Research

Viruses ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 324 ◽  
Author(s):  
Jian Jiang ◽  
Junfei Ma ◽  
Bin Liu ◽  
Ying Wang
Keyword(s):  
Molecular Biology ◽  
Structure Function ◽  
High Throughput ◽  
Noncoding Rnas ◽  
Protein Precipitation ◽  
New Method ◽  
Robust Method ◽  
Simple Method ◽  
Functional Studies ◽  
Arabidopsis Protoplast

Plant–viroid interactions represent a valuable model for delineating structure–function relationships of noncoding RNAs. For various functional studies, it is desirable to minimize sample variations by using DNA, RNA, and proteins co-purified from the same samples. Currently, most of the co-purification protocols rely on TRI Reagent (Trizol as a common representative) and require protein precipitation and dissolving steps, which render difficulties in experimental handling and high-throughput analyses. Here, we established a simple and robust method to minimize the precipitation steps and yield ready-to-use RNA and protein in solutions. This method can be applied to samples in small quantities, such as protoplasts. Given the ease and the robustness of this new method, it will have broad applications in virology and other disciplines in molecular biology.

scholarly journals A simple method to co-purify genomic DNA, RNA, and proteins for functional studies

2018 ◽  
Author(s):  
Jian Jiang ◽  
Junfei Ma ◽  
Bin Liu ◽  
Ying Wang
Keyword(s):  
Gene Expression ◽  
Molecular Biology ◽  
High Throughput ◽  
Genomic Dna ◽  
Molecular Mechanisms ◽  
Rapid Development ◽  
Regulation Of Gene Expression ◽  
Robust Method ◽  
Simple Method ◽  
Functional Studies

AbstractUnderstanding the regulation of gene expression, from the epigenetic modifications on genomes to posttranscriptional and translational controls, are critical for elucidating molecular mechanisms underlying distinct phenotypes in biology. With the rapid development of Multi-Omics analyses, it is desirable to minimize sample variations by using DNA, RNA, and proteins co-purified from the same samples. Currently, most of the co-purification protocols rely on Tri Reagent (Trizol as a common representative) and require protein precipitation and dissolving steps, which render difficulties in experimental handling and high-throughput analyses. Here, we established a simple and robust method to minimize the precipitation steps and yield ready-to-use RNA and protein in solutions. This method can be applied to samples in small quantity, such as protoplasts. We demonstrated that the protoplast system equipped with this method may facilitate studies on viroid biogenesis. Given the ease and the robustness of this new method, it will have broad applications for plant research and other disciplines in molecular biology.

scholarly journals Nitrocellulose redox permanganometry: a simple method for reductive capacity assessment

2020 ◽  
Author(s):  
J Homolak ◽  
I Kodvanj ◽  
A Babic Perhoc ◽  
D Virag ◽  
A Knezovic ◽  
...  
Keyword(s):  
Spatial Distribution ◽  
Detailed Analysis ◽  
Manganese Dioxide ◽  
High Throughput ◽  
Biological Samples ◽  
Capacity Assessment ◽  
Robust Method ◽  
Nitrocellulose Membrane ◽  
High Throughput Analysis ◽  
Simple Method

AbstractWe propose a rapid, simple and robust method for measurement of reductive capacity of liquid and solid biological samples based on potassium permanganate reduction followed by trapping of manganese dioxide precipitate on a nitrocellulose membrane. Moreover, we discuss how nitrocellulose redox permanganometry (NRP) can be used for high-throughput analysis of biological samples and present HistoNRP, its modification used for detailed analysis of reductive capacity spatial distribution in tissue with preserved anatomical relations.

Capturing Protein Droplets: Label-free Visualization and Detection of Protein Liquid-Liquid Phase Separation with an Aggregation-Induced Emission Fluorogen

2021 ◽  
Author(s):  
Yan-Mei Li ◽  
Chu-Qiao Liang ◽  
Lin Wang ◽  
Yun-Yi Luo ◽  
Qian-Qian Li
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Liquid Phase Separation ◽  
New Method ◽  
Label Free ◽  
Simple Method ◽  
Aggregation Induced Emission ◽  
Liquid Liquid Phase Separation

We developed a new method for protein droplet visualization by means of a droplet probe (DroProbe) based on an aggregation-induced emission (AIE) fluorogen. A simple method for viscosity comparison of...

Robust Method for High-Throughput Surface Patterning of Deformable Substrates

Langmuir ◽  
2011 ◽  
Vol 27 (12) ◽  
pp. 7349-7352 ◽  
Author(s):  
Ammar Azioune ◽  
Nicolas Carpi ◽  
Jenny Fink ◽  
Mohamed M. Chehimi ◽  
Damien Cuvelier ◽  
...  
Keyword(s):  
High Throughput ◽  
Surface Patterning ◽  
Robust Method

scholarly journals A simple new method for identifying performance characteristics associated with success in elite tennis

2018 ◽  
Vol 14 (1) ◽  
pp. 43-50 ◽  
Author(s):  
Anna Fitzpatrick ◽  
Joseph A Stone ◽  
Simon Choppin ◽  
John Kelley
Keyword(s):  
Performance Analysis ◽  
Statistical Methods ◽  
Rank Order ◽  
Large Datasets ◽  
Performance Characteristics ◽  
New Method ◽  
Considerable Time ◽  
Simple Method ◽  
Suitable Alternative ◽  
Using Data

Performance analysis and identifying performance characteristics associated with success are of great importance to players and coaches in any sport. However, while large amounts of data are available within elite tennis, very few players employ an analyst or attempt to exploit the data to enhance their performance; this is partly attributable to the considerable time and complex techniques required to interpret these large datasets. Using data from the 2016 and 2017 French Open tournaments, we tested the agreement between the results of a simple new method for identifying important performance characteristics (the Percentage of matches in which the Winner Outscored the Loser, PWOL) and the results of two standard statistical methods to establish the validity of the simple method. Spearman’s rank-order correlations between the results of the three methods demonstrated excellent agreement, with all methods identifying the same three performance characteristics ( points won of 0–4 rally length, baseline points won and first serve points won) as strongly associated with success. Consequently, we propose that the PWOL method is valid for identifying performance characteristics associated with success in tennis, and is therefore a suitable alternative to more complex statistical methods, as it is simpler to calculate, interpret and contextualise.

scholarly journals Integrating Molecular Biology and Bioinformatics Education

2018 ◽  
Author(s):  
Boas Pucker ◽  
Hanna Marie Schilbert ◽  
Sina Franziska Schumacher
Keyword(s):  
Molecular Biology ◽  
Cooperative Learning ◽  
Peer Review ◽  
High Throughput ◽  
Teaching Method ◽  
Integrated Education ◽  
High Throughput Data ◽  
Wet Lab ◽  
Bioinformatics Education ◽  
Increasing Demand

Combined awareness about the power and limitations of bioinformatics and molecular biology enables advanced research based on high-throughput data. Despite an increasing demand for scientists with a combined background in both fields, the education in dry lab and wet lab is often separated. This work describes an example of integrated education with focus on genomics and transcriptomics. Participants learn computational and molecular biology methods in the same practical course. Peer-review is applied as a teaching method to foster cooperative learning of students with heterogeneous backgrounds. Evaluation results indicate acceptance and appreciation of this approach.

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