scholarly journals Nitrocellulose redox permanganometry: a simple method for reductive capacity assessment

2020 ◽  
Author(s):  
J Homolak ◽  
I Kodvanj ◽  
A Babic Perhoc ◽  
D Virag ◽  
A Knezovic ◽  
...  
Keyword(s):  
Spatial Distribution ◽  
Detailed Analysis ◽  
Manganese Dioxide ◽  
High Throughput ◽  
Biological Samples ◽  
Capacity Assessment ◽  
Robust Method ◽  
Nitrocellulose Membrane ◽  
High Throughput Analysis ◽  
Simple Method

AbstractWe propose a rapid, simple and robust method for measurement of reductive capacity of liquid and solid biological samples based on potassium permanganate reduction followed by trapping of manganese dioxide precipitate on a nitrocellulose membrane. Moreover, we discuss how nitrocellulose redox permanganometry (NRP) can be used for high-throughput analysis of biological samples and present HistoNRP, its modification used for detailed analysis of reductive capacity spatial distribution in tissue with preserved anatomical relations.

Microfluidic array surface ion-imprinted monolithic capillary microextraction chip on-line hyphenated with ICP-MS for the high throughput analysis of gadolinium in human body fluids

The Analyst ◽  
2019 ◽  
Vol 144 (8) ◽  
pp. 2736-2745 ◽  
Author(s):  
Xiaoxiao Ou ◽  
Man He ◽  
Beibei Chen ◽  
Han Wang ◽  
Bin Hu
Keyword(s):  
High Throughput ◽  
Human Body ◽  
Biological Samples ◽  
Body Fluids ◽  
High Throughput Analysis ◽  
Capillary Microextraction ◽  
Icp Ms ◽  
Ion Imprinted ◽  
On Line ◽  
Novel Method

A novel method by hyphenating chip-based array ion-imprinted monolithic capillary microextraction with ICP-MS was proposed for the online analysis of trace Gd in biological samples.

scholarly journals Combining a Simple Method for DNA/RNA/Protein Co-Purification and Arabidopsis Protoplast Assay to Facilitate Viroid Research

Viruses ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 324 ◽  
Author(s):  
Jian Jiang ◽  
Junfei Ma ◽  
Bin Liu ◽  
Ying Wang
Keyword(s):  
Molecular Biology ◽  
Structure Function ◽  
High Throughput ◽  
Noncoding Rnas ◽  
Protein Precipitation ◽  
New Method ◽  
Robust Method ◽  
Simple Method ◽  
Functional Studies ◽  
Arabidopsis Protoplast

Plant–viroid interactions represent a valuable model for delineating structure–function relationships of noncoding RNAs. For various functional studies, it is desirable to minimize sample variations by using DNA, RNA, and proteins co-purified from the same samples. Currently, most of the co-purification protocols rely on TRI Reagent (Trizol as a common representative) and require protein precipitation and dissolving steps, which render difficulties in experimental handling and high-throughput analyses. Here, we established a simple and robust method to minimize the precipitation steps and yield ready-to-use RNA and protein in solutions. This method can be applied to samples in small quantities, such as protoplasts. Given the ease and the robustness of this new method, it will have broad applications in virology and other disciplines in molecular biology.

scholarly journals A simple method for squeezing juice from rice stems and its use in the high-throughput analysis of sugar content in rice stems

2016 ◽  
Vol 19 (2) ◽  
pp. 309-314 ◽  
Author(s):  
Masaki Okamura ◽  
Yoichi Hashida ◽  
Tatsuro Hirose ◽  
Ryu Ohsugi ◽  
Naohiro Aoki
Keyword(s):  
High Throughput ◽  
Sugar Content ◽  
High Throughput Analysis ◽  
Simple Method ◽  
Throughput Analysis

A 3D-printed modularized purification system for rapid, high-throughput MALDI-MS analysis of small-volume biological samples

2020 ◽  
Vol 56 (11) ◽  
pp. 1637-1640
Author(s):  
Xiu Huang ◽  
Dingyi Wang ◽  
Bin He ◽  
Qian Liu ◽  
Ligang Hu ◽  
...  
Keyword(s):  
High Throughput ◽  
Biological Samples ◽  
Small Volume ◽  
Maldi Ms ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
Ms Analysis ◽  
Purification System ◽  
3D Printed ◽  
Sample Purification

A modularized sample purification system was 3D printed for rapid, high-throughput analysis of small-volume biological samples.

scholarly journals High Throughput Procedure for Comparative Analysis of In Vivo Cardiac Glucose or Amino Acids Use in Cardiovascular Pathologies and Pharmacological Treatments

Metabolites ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 497
Author(s):  
Marta Tomczyk ◽  
Mariola Olkowicz ◽  
Ewa M. Slominska ◽  
Ryszard T. Smolenski
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Energy Metabolism ◽  
High Throughput ◽  
Cardiac Metabolism ◽  
Spectrometric Analysis ◽  
Pharmacological Treatments ◽  
High Throughput Analysis ◽  
Simple Method

The heart is characterized by the prominent flexibility of its energy metabolism and is able to use diverse carbon substrates, including carbohydrates and amino acids. Cardiac substrate preference could have a major impact on the progress of cardiac pathologies. However, the majority of methods to investigate changes in substrates’ use in cardiac metabolism in vivo are complex and not suitable for high throughput testing necessary to understand and reverse these pathologies. Thus, this study aimed to develop a simple method that would allow for the analysis of cardiac metabolic substrate use. The developed methods involved the subcutaneous injection of stable 13C isotopomers of glucose, valine, or leucine with mass spectrometric analysis for the investigation of its entry into cardiac metabolic pathways that were deducted from 13C alanine and glutamate enrichments in heart extracts. The procedures were validated by confirming the known effects of treatments that modify glucose, free fatty acids, and amino acid metabolism. Furthermore, we studied changes in the energy metabolism of CD73 knock-out mice to demonstrate the potential of our methods in experimental research. The methods created allowed for fast estimation of cardiac glucose and amino acid use in mice and had the potential for high-throughput analysis of changes in pathology and after pharmacological treatments.

High-throughput analysis of biological samples by direct coupling of solid-phase (micro-)extraction and mass spectrometry

2002 ◽  
Vol 55 (S1) ◽  
pp. S23-S24 ◽  
Author(s):  
M. W. J. van Hout ◽  
C. M. Hofland ◽  
V. Jas ◽  
H. A. G. Niederländer ◽  
R. A. de Zeeuw ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
Biological Samples ◽  
Solid Phase ◽  
Direct Coupling ◽  
Solid Phase Micro Extraction ◽  
High Throughput Analysis ◽  
Throughput Analysis

scholarly journals A Robust Method for Quantitative High-throughput Analysis of Proteomes by18O Labeling

2010 ◽  
Vol 10 (1) ◽  
pp. M110.003335 ◽  
Author(s):  
Elena Bonzon-Kulichenko ◽  
Daniel Pérez-Hernández ◽  
Estefanía Núñez ◽  
Pablo Martínez-Acedo ◽  
Pedro Navarro ◽  
...  
Keyword(s):  
High Throughput ◽  
Robust Method ◽  
High Throughput Analysis ◽  
Throughput Analysis

scholarly journals Generation of barcoded plasmid libraries for massively parallel analysis of chromatin position effects

2019 ◽  
Vol 23 (2) ◽  
pp. 203-211
Author(s):  
M. O. Lebedev ◽  
L. A. Yarinich ◽  
A. V. Ivankin ◽  
A. V. Pindyurin
Keyword(s):  
High Throughput ◽  
Molecular Mechanisms ◽  
Position Effect ◽  
Critical Parameters ◽  
Position Effect Variegation ◽  
High Throughput Analysis ◽  
Simple Method ◽  
Position Effects ◽  
Gibson Assembly ◽  
Wide Range

The discovery of the position effect variegation phenomenon and the subsequent comprehensive analysis of its molecular mechanisms led to understanding that the local chromatin composition has a dramatic effect on gene activity. To study this effect in a high-throughput mode and at the genome-wide level, the Thousands of Reporters Integrated in Parallel (TRIP) approach based on the usage of barcoded reporter gene constructs was recently developed. Here we describe the construction and quality checks of high-diversity barcoded plasmid libraries supposed to be used for high-throughput analysis of chromatin position effects in Drosophila cells. First, we highlight the critical parameters that should be considered in the generation of barcoded plasmid libraries and introduce a simple method to assess the diversity of random sequences (barcodes) of synthetic oligonucleotides using PCR amplification followed by Sanger sequencing. Second, we compare the conventional restriction-ligation method with the Gibson assembly approach for cloning barcodes into the same plasmid vector. Third, we provide optimized parameters for the construction of barcoded plasmid libraries, such as the vector : insert ratio in the Gibson assembly reaction and the voltage used for electroporation of bacterial cells with ligation products. We also compare different approaches to check the quality of barcoded plasmid libraries. Finally, we briefly describe alternative approaches that can be used for the generation of such libraries. Importantly, all improvements and modifications of the techniques described here can be applied to a wide range of experiments involving barcoded plasmid libraries.

scholarly journals A simple method to co-purify genomic DNA, RNA, and proteins for functional studies

2018 ◽  
Author(s):  
Jian Jiang ◽  
Junfei Ma ◽  
Bin Liu ◽  
Ying Wang
Keyword(s):  
Gene Expression ◽  
Molecular Biology ◽  
High Throughput ◽  
Genomic Dna ◽  
Molecular Mechanisms ◽  
Rapid Development ◽  
Regulation Of Gene Expression ◽  
Robust Method ◽  
Simple Method ◽  
Functional Studies

AbstractUnderstanding the regulation of gene expression, from the epigenetic modifications on genomes to posttranscriptional and translational controls, are critical for elucidating molecular mechanisms underlying distinct phenotypes in biology. With the rapid development of Multi-Omics analyses, it is desirable to minimize sample variations by using DNA, RNA, and proteins co-purified from the same samples. Currently, most of the co-purification protocols rely on Tri Reagent (Trizol as a common representative) and require protein precipitation and dissolving steps, which render difficulties in experimental handling and high-throughput analyses. Here, we established a simple and robust method to minimize the precipitation steps and yield ready-to-use RNA and protein in solutions. This method can be applied to samples in small quantity, such as protoplasts. We demonstrated that the protoplast system equipped with this method may facilitate studies on viroid biogenesis. Given the ease and the robustness of this new method, it will have broad applications for plant research and other disciplines in molecular biology.

Re-appraising and Re-classifying: a New Look at the Corpus of Miniature Socketed Axes from Britain

2020 ◽  
Vol 75 (1) ◽  
pp. 1-27
Author(s):  
Alex Bliss
Keyword(s):  
Spatial Distribution ◽  
Data Collection ◽  
Detailed Analysis ◽  
Significant Proportion ◽  
Direct Result ◽  
Object Type ◽  
England And Wales ◽  
Extensive Data ◽  
Metal Artefacts ◽  
The Subject

The advent of the Portable Antiquities Scheme (PAS) has added a great deal to our understanding of prehistoric metal artefacts in England and Wales, namely in expanding enormously the corpuses of objects previously thought to be quite scarce. One such artefact type is the miniature socketed 'votive' axe, most of which are found in Wiltshire and Hampshire. As a direct result of developing such recording initiatives, reporting of these artefacts as detector finds from the early 2000s onwards has virtually trebled the number originally published by Paul Robinson in his 1995 analysis. Through extensive data-collection, synthesising examples recorded via the PAS with those from published excavations, the broad aims of this paper (in brief) are as follows: firstly, produce a solid typology for these artefacts; secondly, investigate their spatial distribution across England and Wales. As a more indirect third aim, this paper also seeks to redress the imbalance of focus and academic study specifically applying to Hampshire finds of this object type, which despite producing a significant proportion of the currently known corpus have never been the subject of detailed analysis.

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