scholarly journals Tick-Borne Encephalitis Virus Adaptation in Different Host Environments and Existence of Quasispecies

Viruses ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 902
Author(s):  
Renata Helmová ◽  
Václav Hönig ◽  
Hana Tykalová ◽  
Martin Palus ◽  
Lesley Bell-Sakyi ◽  
...  
Keyword(s):  
Virulent Strain ◽  
Encephalitis Virus ◽  
Genome Sequences ◽  
Tick Borne Encephalitis ◽  
Ricinus Tick ◽  
Tick Borne Encephalitis Virus ◽  
Genomic Regions ◽  
Virus Adaptation ◽  
Virus Survival ◽  
Highly Virulent

A highly virulent strain (Hypr) of tick-borne encephalitis virus (TBEV) was serially subcultured in the mammalian porcine kidney stable (PS) and Ixodes ricinus tick (IRE/CTVM19) cell lines, producing three viral variants. These variants exhibited distinct plaque sizes and virulence in a mouse model. Comparing the full-genome sequences of all variants, several nucleotide changes were identified in different genomic regions. Furthermore, different sequential variants were revealed to co-exist within one sample as quasispecies. Interestingly, the above-mentioned nucleotide changes found within the whole genome sequences of the new variants were present alongside the nucleotide sequence of the parental strain, which was represented as a minority quasispecies. These observations further imply that TBEV exists as a heterogeneous population that contains virus variants pre-adapted to reproduction in different environments, probably enabling virus survival in ticks and mammals.

Full genome sequences and molecular characterization of tick-borne encephalitis virus strains isolated from human patients

2015 ◽  
Vol 6 (1) ◽  
pp. 38-46 ◽  
Author(s):  
Petra Formanová ◽  
Jiří Černý ◽  
Barbora Černá Bolfíková ◽  
James J. Valdés ◽  
Irina Kozlova ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Encephalitis Virus ◽  
Full Genome ◽  
Genome Sequences ◽  
Tick Borne Encephalitis ◽  
Tick Borne Encephalitis Virus ◽  
Virus Strains

scholarly journals Characterization and complete genome sequences of high- and low-virulence variants of tick-borne encephalitis virus

1996 ◽  
Vol 77 (5) ◽  
pp. 1035-1042 ◽  
Author(s):  
G. Wallner ◽  
C. W. Mandl ◽  
M. Ecker ◽  
H. Holzmann ◽  
K. Stiasny ◽  
...  
Keyword(s):  
Complete Genome ◽  
Encephalitis Virus ◽  
Genome Sequences ◽  
Tick Borne Encephalitis ◽  
Tick Borne Encephalitis Virus

Ixodes ricinus tick saliva modulates tick-borne encephalitis virus infection of dendritic cells

2010 ◽  
Vol 12 (7) ◽  
pp. 580-585 ◽  
Author(s):  
Anna Fialová ◽  
Zdeněk Cimburek ◽  
Giandomenica Iezzi ◽  
Jan Kopecký
Keyword(s):  
Dendritic Cells ◽  
Virus Infection ◽  
Ixodes Ricinus ◽  
Encephalitis Virus ◽  
Tick Saliva ◽  
Tick Borne Encephalitis ◽  
Ricinus Tick ◽  
Tick Borne Encephalitis Virus

scholarly journals Complete Genome Sequences of Four European Subtype Strains of Tick-Borne Encephalitis Virus from Eastern Siberia, Russia

2015 ◽  
Vol 3 (3) ◽  
Author(s):  
Renat V. Adelshin ◽  
Olga V. Melnikova ◽  
Ludmila S. Karan ◽  
Evgeny I. Andaev ◽  
Sergey V. Balakhonov
Keyword(s):  
Complete Genome ◽  
Encephalitis Virus ◽  
Eastern Siberia ◽  
Genome Sequences ◽  
Tick Borne Encephalitis ◽  
Tick Borne Encephalitis Virus ◽  
European Subtype

scholarly journals Ixodes scapularis and Ixodes ricinus tick cell lines respond to infection with tick-borne encephalitis virus: transcriptomic and proteomic analysis

2015 ◽  
Vol 8 (1) ◽  
Author(s):  
Sabine Weisheit ◽  
Margarita Villar ◽  
Hana Tykalová ◽  
Marina Popara ◽  
Julia Loecherbach ◽  
...  
Keyword(s):  
Cell Lines ◽  
Ixodes Ricinus ◽  
Proteomic Analysis ◽  
Ixodes Scapularis ◽  
Encephalitis Virus ◽  
Tick Cell ◽  
Tick Borne Encephalitis ◽  
Ricinus Tick ◽  
Tick Borne Encephalitis Virus

scholarly journals CHARACTERISTICS OF THE FAR EASTERN POPULATION OF TICK-BORNE ENCEPHALITIS VIRUS AND SOLVING ISSUES OF SPECIFIC PREVENTION

2018 ◽  
Vol 3 (4) ◽  
pp. 42-46
Author(s):  
G. N. Leonova
Keyword(s):  
Virulent Strain ◽  
Biological Properties ◽  
Encephalitis Virus ◽  
Viral Population ◽  
Full Genome Sequencing ◽  
Genetic Characteristics ◽  
Specific Antibodies ◽  
Tick Borne Encephalitis ◽  
Tick Borne Encephalitis Virus ◽  
Far Eastern

The subdivision of the viral population into subtypes and clusters based on the molecular genetic characteristics of the tick-borne  encephalitis virus (TBEV) strains predetermines not only the  differences in the biological properties of these strains, but also their different responses to specific antibodies in persons vaccinated against TBE.The aim of the present study is to show the differences in biological properties on the model of two strains of Far Eastern TBEV subtype  belonging to different clusters and to substantiate the need for a  personalized approach to the vaccine prophylaxis of tick-borne encephalitis.Results. Two strains of TBEV were used in the studies. On the basis of full genome sequencing the Dal’negorsk strain (FJ402886,  GenBank) is referred to the typical representative of Sofjin-like, and  Primorye-437 (JQ825162, GenBank) – to Oshima-like TBEV strains  of the Far Eastern subtype.The experiment shows the levels of  specific antibodies capable of neutralizing virulence strains of tick- borne encephalitis virus. Low antibody titers (1:100 and 1:400) can  neutralize only a low dose of a non-virulent strain of tick-borne  encephalitis virus. Reliable protection against the disease of people infected with virulent strains of the TBEV can provide only a  high level of specific antibodies.Conclusion. If the level of specific antibodies of IgG is 1:400 or lower, the vaccination course should be continued, at a titer of  antibodies above 1:400, revaccination can be postponed subject to  annual monitoring of specific immunity parameters in the pre-epidemic TBE season.

TBE in Bulgaria

2021 ◽  
Author(s):  
Iva Christova
Keyword(s):  
Virulent Strain ◽  
Goat Milk ◽  
Tick Borne Encephalitis ◽  
Antigenic Properties ◽  
Haemaphysalis Punctata ◽  
Tick Borne Encephalitis Virus ◽  
The 1960S ◽  
Virus Strains ◽  
European Subtype ◽  
Highly Virulent

First cases of probable tick-borne encephalitis (TBE) were reported in 1961 by Andonov et al. in eastern regions of Bulgaria.1 Possible TBE cases with the typical two-wave fever, originating from consumption of raw goat milk, were described back in 1953 by Vaptzarov et al. in southern Bulgaria.2 Investigations in the 1960s were able to isolate 3 tick-borne encephalitis virus (TBEV) strains from Haemaphysalis punctata and 1 from Dermacentor marginatus ticks from goats and sheep in the district of Plovdiv.3 The antigenic properties of these 4 virus strains were identical to the highly virulent strain “Hypr” of the European subtype of TBEV (TBEV-EU).

scholarly journals Chimeric Langat/Dengue Viruses Protect Mice from Heterologous Challenge with the Highly Virulent Strains of Tick-Borne Encephalitis Virus

Virology ◽  
2000 ◽  
Vol 274 (1) ◽  
pp. 26-31 ◽  
Author(s):  
Alexander G. Pletnev ◽  
Galina G. Karganova ◽  
Tatiana I. Dzhivanyan ◽  
Vasilii A. Lashkevich ◽  
Michael Bray
Keyword(s):  
Encephalitis Virus ◽  
Dengue Viruses ◽  
Virulent Strains ◽  
Tick Borne Encephalitis ◽  
Heterologous Challenge ◽  
Tick Borne Encephalitis Virus ◽  
Highly Virulent

Full genome sequences and preliminary molecular characterization of three tick-borne encephalitis virus strains isolated from ticks and a bank vole in Slovak Republic

Virus Genes ◽  
2013 ◽  
Vol 48 (1) ◽  
pp. 184-188 ◽  
Author(s):  
Stefan Frey ◽  
Sandra Essbauer ◽  
Gudrun Zöller ◽  
Boris Klempa ◽  
Gerhard Dobler ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Bank Vole ◽  
Slovak Republic ◽  
Encephalitis Virus ◽  
Full Genome ◽  
Genome Sequences ◽  
Tick Borne Encephalitis ◽  
Tick Borne Encephalitis Virus ◽  
Virus Strains

scholarly journals Antiviral Activity of Uridine Derivatives of 2-Deoxy Sugars against Tick-Borne Encephalitis Virus

Molecules ◽  
2019 ◽  
Vol 24 (6) ◽  
pp. 1129 ◽  
Author(s):  
Ewelina Krol ◽  
Ilona Wandzik ◽  
Gabriela Brzuska ◽  
Luděk Eyer ◽  
Daniel Růžek ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Virulent Strain ◽  
Encephalitis Virus ◽  
Antiviral Therapies ◽  
Tick Borne Encephalitis ◽  
Tbev Strain ◽  
Ic50 Values ◽  
Tick Borne Encephalitis Virus ◽  
Derivatives Of ◽  
Deoxy Sugars

Tick-borne encephalitis virus (TBEV) is a causative agent of tick-borne encephalitis (TBE), one of the most important human infections involving the central nervous system. Although effective vaccines are available on the market, they are recommended only in endemic areas. Despite many attempts, there are still no specific antiviral therapies for TBEV treatment. Previously, we synthesized a series of uridine derivatives of 2-deoxy sugars and proved that some compounds show antiviral activity against viruses from the Flaviviridae and Orthomyxoviridae families targeting the late steps of the N-glycosylation process, affecting the maturation of viral proteins. In this study, we evaluated a series of uridine derivatives of 2-deoxy sugars for their antiviral properties against two strains of the tick-borne encephalitis virus; the highly virulent TBEV strain Hypr and the less virulent strain Neudoerfl. Four compounds (2, 4, 10, and 11) showed significant anti-TBEV activity with IC50 values ranging from 1.4 to 10.2 µM and low cytotoxicity. The obtained results indicate that glycosylation inhibitors, which may interact with glycosylated membrane TBEV E and prM proteins, might be promising candidates for future antiviral therapies against TBEV.

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