scholarly journals A Canine Distemper Virus Retrospective Study Conducted from 2011 to 2019 in Central Italy (Latium and Tuscany Regions)

Viruses ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 272 ◽  
Author(s):  
Ida Ricci ◽  
Antonella Cersini ◽  
Giuseppe Manna ◽  
Gaetana Anita Marcario ◽  
Raffaella Conti ◽  
...  
Keyword(s):  
Canine Distemper Virus ◽  
Rectal Swab ◽  
Central Italy ◽  
The Arctic ◽  
Passive Surveillance ◽  
Canine Distemper ◽  
Viral Pathogen ◽  
Nucleoprotein Gene ◽  
Wild Canids ◽  
Reported Data

Canine distemper virus (CDV) is a highly lethal contagious viral pathogen mainly found in domestic and wild canids and mustelids. Although, in Italy, circulating strains of Europe 1, Europe wildlife and Arctic type are reported, data relating to Latium and Tuscany regions are limited. In view of this, through passive surveillance, we investigated the presence of CDV and which strains were circulating in these Regions. From March 2017 to October 2019, a group of 122 subjects were tested for CDV using a PCR protocol described in the literature, with 12 detected positive; analyses were carried out on a set of target samples (brain and lung, conjunctival, nasal and rectal swabs, urine or swab from bladder and intracardiac clot) that was defined for the detection of CDV in both live and dead animals. The rectal swab, easily collected also from live animals, represented the most suitable sample for CDV diagnosis, with 9 positive of the 11 (81.82%) tested. In addition, brain and lung of 15 subjects out of 181 susceptible animals collected between 2011 and 2018, during post mortem investigations in routine diagnostic activity, were CDV positive. Molecular analyses of all positive samples, using a 287 bp fragment located within the conserved N terminus of the morbillivirus nucleoprotein gene, detected the circulation of strain CDV599/2016 (KX545421.1) belonging to the “Europe wildlife” lineage, and of strain CDV12254/2015 (KX024709.1), belonging to the Arctic-lineage, thus confirming the co-circulation of the two lineages, as already noted in previous studies.

scholarly journals EUTHANASIA OF ACUTE NEUROLGIC CANINE DISTEMPER VIRUS AFFECTED PET DOG ADJACENT TO PROTECTED FOREST AREA

2020 ◽  
Vol 4 (2) ◽  
pp. 67-68
Author(s):  
Vimalraj Padayatchiar Govindan ◽  
Parag Madhukar Dhakate ◽  
Ayush Uniyal
Keyword(s):  
Adult Male ◽  
Canine Distemper Virus ◽  
Clinical Signs ◽  
Signs And Symptoms ◽  
Canine Distemper ◽  
Muscle Twitching ◽  
Head Nodding ◽  
Wild Canids ◽  
History Of ◽  
Clinical Signs And Symptoms

Adult male non-descript bred dog presented with a history of circling motion, frequent head nodding, crusty muzzle, muscle twitching, seizure, vomiting, inappetence, coughing, inability to walk, watery pustular discharge from eyes and blood stained left ears and on further examination of foot pads showed thick, crusty or hardened sole and based on the clinical signs and symptoms, provisionally diagnosed as Canine distemper and advised euthanasia due to close geographic onset of the disease epidemic and to prevent Canine distemper-associated deaths in wild canids and felids.

scholarly journals Canine Distemper Virus Strains Circulating among North American Dogs

2008 ◽  
Vol 15 (4) ◽  
pp. 707-712 ◽  
Author(s):  
Sanjay Kapil ◽  
Robin W. Allison ◽  
Larry Johnston ◽  
Brandy L. Murray ◽  
Steven Holland ◽  
...  
Keyword(s):  
Canine Distemper Virus ◽  
Lymphocyte Activation ◽  
The United States ◽  
The Arctic ◽  
Canine Distemper ◽  
Genetic Groups ◽  
Group I ◽  
Current Vaccine ◽  
Vero Cell Line ◽  
Multisystemic Disease

ABSTRACT Canine distemper virus (CDV) is a highly contagious virus that causes multisystemic disease in dogs. We received seven samples from dogs with CD from the United States during 2007. CDV isolates from these samples formed large, multinucleated syncytia in a Vero cell line expressing canine signaling lymphocyte activation molecule (SLAM). Based on the hemagglutinin gene sequences, the CDV isolates from three states (California, Missouri, and Oklahoma) formed two CDV genetic groups: group I (major; six of seven isolates) consisted of CDV isolates closely related to the European wildlife lineage of CDV, and group II (minor; one of seven isolates) was genetically related to the Arctic-like lineage of CDV. However, both CDV groups were genetically different from the current vaccine strains that belong to the American-1 lineage of the old (1930 to 1950) CDV isolates.

scholarly journals Prolonged Infection of Canine Distemper Virus in a Mixed-Breed Dog

2021 ◽  
Vol 8 (4) ◽  
pp. 61
Author(s):  
Zsófia Lanszki ◽  
Brigitta Zana ◽  
Safia Zeghbib ◽  
Ferenc Jakab ◽  
Nikoletta Szabó ◽  
...  
Keyword(s):  
Canine Distemper Virus ◽  
Dirofilaria Immitis ◽  
Genetic Lineage ◽  
Canine Distemper ◽  
Viral Pathogen ◽  
Mixed Breed ◽  
Wide Range ◽  
Canine Infection ◽  
Vector Borne ◽  
Long Course

Canine distemper virus (CDV) is a major viral pathogen in domestic dogs, belonging to the Paramyxoviridae family, in the Morbillivirus genus. It is present worldwide, and a wide range of domestic animals and wild carnivores are at risk. In the absence of vaccination, dogs have a low chance of survival; however, if and when a dog survives, it can take an average of a few weeks to a few months to fully wipe out the virus. In the present study, we traced the course of infection of a 1-year-old mixed-breed male dog. The animal had an unusually long course of persistent CDV infection with a vector-borne heartworm (Dirofilaria immitis) co-infection. The dog excreted the CDV for 17 months with PCR positivity in urine samples collected from February 2019 through June 2020. The sequencing and phylogenetic analysis of the hemagglutinin gene revealed the CDV to be the member of the endemic Arctic-like genetic lineage. To the best of our knowledge, this report represents the longest documented canine infection of CDV. Notably, we highlight the necessity regarding CDV infectivity studies to better comprehend the transmission attributes of the virus.

scholarly journals Intratumoral Canine Distemper Virus Infection Inhibits Tumor Growth by Modulation of the Tumor Microenvironment in a Murine Xenograft Model of Canine Histiocytic Sarcoma

2021 ◽  
Vol 22 (7) ◽  
pp. 3578
Author(s):  
Federico Armando ◽  
Adnan Fayyad ◽  
Stefanie Arms ◽  
Yvonne Barthel ◽  
Dirk Schaudien ◽  
...  
Keyword(s):  
Tumor Microenvironment ◽  
Virus Infection ◽  
Tumor Growth ◽  
Canine Distemper Virus ◽  
Xenograft Model ◽  
Histiocytic Sarcoma ◽  
Oncolytic Viruses ◽  
Canine Distemper ◽  
Murine Xenograft Model ◽  
The Impact

Histiocytic sarcomas refer to highly aggressive tumors with a poor prognosis that respond poorly to conventional treatment approaches. Oncolytic viruses, which have gained significant traction as a cancer therapy in recent decades, represent a promising option for treating histiocytic sarcomas through their replication and/or by modulating the tumor microenvironment. The live attenuated canine distemper virus (CDV) vaccine strain Onderstepoort represents an attractive candidate for oncolytic viral therapy. In the present study, oncolytic virotherapy with CDV was used to investigate the impact of this virus infection on tumor cell growth through direct oncolytic effects or by virus-mediated modulation of the tumor microenvironment with special emphasis on angiogenesis, expression of selected MMPs and TIMP-1 and tumor-associated macrophages in a murine xenograft model of canine histiocytic sarcoma. Treatment of mice with xenotransplanted canine histiocytic sarcomas using CDV induced overt retardation in tumor progression accompanied by necrosis of neoplastic cells, increased numbers of intratumoral macrophages, reduced angiogenesis and modulation of the expression of MMPs and TIMP-1. The present data suggest that CDV inhibits tumor growth in a multifactorial way, including direct cell lysis and reduction of angiogenesis and modulation of MMPs and their inhibitor TIMP-1, providing further support for the concept of its role in oncolytic therapies.

scholarly journals Drivers of canine distemper virus exposure in dogs at a wildlife interface in Janos, Mexico

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Rocío Almuna ◽  
Andrés M. López‐Pérez ◽  
Rosa E. Sarmiento ◽  
Gerardo Suzán
Keyword(s):  
Canine Distemper Virus ◽  
Canine Distemper ◽  
Virus Exposure

scholarly journals Antiviral Screen against Canine Distemper Virus-Induced Membrane Fusion Activity

Viruses ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 128
Author(s):  
Neeta Shrestha ◽  
Flavio M. Gall ◽  
Jonathan Vesin ◽  
Marc Chambon ◽  
Gerardo Turcatti ◽  
...  
Keyword(s):  
Membrane Fusion ◽  
Small Molecule ◽  
High Throughput Screening ◽  
Measles Virus ◽  
Canine Distemper Virus ◽  
Rna Virus ◽  
Preventive Measure ◽  
Close Relative ◽  
Fusion Activity ◽  
Canine Distemper

Canine distemper virus (CDV), a close relative of the human pathogen measles virus (MeV), is an enveloped, negative sense RNA virus that belongs to the genus Morbillivirus and causes severe diseases in dogs and other carnivores. Although the vaccination is available as a preventive measure against the disease, the occasional vaccination failure highlights the importance of therapeutic alternatives such as antivirals against CDV. The morbilliviral cell entry system relies on two interacting envelope glycoproteins: the attachment (H) and fusion (F) proteins. Here, to potentially discover novel entry inhibitors targeting CDV H, F and/or the cognate receptor: signaling lymphocyte activation molecule (SLAM) proteins, we designed a quantitative cell-based fusion assay that matched high-throughput screening (HTS) settings. By screening two libraries of small molecule compounds, we successfully identified two membrane fusion inhibitors (F2736-3056 and F2261-0043). Although both inhibitors exhibited similarities in structure and potency with the small molecule compound 3G (an AS-48 class morbilliviral F-protein inhibitor), F2736-3056 displayed improved efficacy in blocking fusion activity when a 3G-escape variant was employed. Altogether, we present a cell-based fusion assay that can be utilized not only to discover antiviral agents against CDV but also to dissect the mechanism of morbilliviral-mediated cell-binding and cell-to-cell fusion activity.

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