scholarly journals Prolonged Viability of Senecavirus A in Exposed House Flies (Musca domestica)

Viruses ◽  
2022 ◽  
Vol 14 (1) ◽  
pp. 127
Author(s):  
Justin Heath Turner ◽  
Willian Pinto Paim ◽  
Mayara Fernanda Maggioli ◽  
Cristina Mendes Peter ◽  
Robert Miknis ◽  
...  
Keyword(s):  
Musca Domestica ◽  
Female Group ◽  
Infectious Dose ◽  
House Flies ◽  
Environmental Resistance ◽  
Successful Isolation ◽  
Swinepox Virus ◽  
High Virus ◽  
Tissue Culture Infectious Dose

House flies (Musca domestica) are often present in swine farms worldwide. These flies utilize animal secretions and waste as a food source. House flies may harbor and transport microbes and pathogens acting as mechanical vectors for diseases. Senecavirus A (SVA) infection in pigs occurs via oronasal route, and animals shed high virus titers to the environment. Additionally, SVA possesses increased environmental resistance. Due to these reasons, we investigated the tenacity of SVA in house flies. Five groups of flies, each composed of ten females and ten males, were exposed to SVA, titer of 109.3 tissue culture infectious dose (TCID50/mL). Groups of male and female flies were collected at 0, 6, 12, 24, and 48 h post-exposure. For comparison purposes, groups of flies were exposed to Swinepox virus (SwPV). Infectious SVA was identified in all tested groups. Successful isolation of SVA demonstrated the titers varied between 106.8 and 102.8 TCID50/mL in female groups and varied from 105.85 to 103.8 TCID50/mL in male groups. In contrast, infectious SwPV was only detected in the female group at 6 h. The significant SVA infectious titer for prolonged periods of time, up to 48 h, indicates a potential role of flies in SVA transmission.

scholarly journals PERANAN Musca domestica SEBAGAI VEKTOR MEKANIK TELUR INFEKTIF Ascaris lumbricoides

2018 ◽  
Vol 2 (1) ◽  
pp. 8
Author(s):  
Suriyani Tan ◽  
Machrumnizar Machrumnizar
Keyword(s):  
Musca Domestica ◽  
Ascaris Lumbricoides ◽  
Light Microscope ◽  
Breeding Site ◽  
Gastrointestinal Diseases ◽  
House Fly ◽  
Animal Waste ◽  
House Flies ◽  
Fish Meat

Muscadomestica (house fly) is an insect that is considered useless by humans although they lived very close to humans. Breeding site of flies in human or animal waste, the rubbish, or unorganic objects that have decayed greatly support their role as mechanical vectors. More than 20 species of flies have been reported as an agent of gastrointestinal diseases. The purpose of this study is to examnine the role of houseflies as mechanical vectors Ascarislumbricoides’seggs.The research sample was 500 house flies (Muscadomestica) captured in the Legok area. Houseflies were trapped by fly trap containing rotten fish meat and then stored at a temperature of 4 degree celcius. The samples were divided into six groups according to the sampling areas, crushed and checked directly by using a light microscope. Ascarislumbricoides eggs are not found in all groups of samples. The study concluded that Muscadomestica is not a mechanical vector of infective eggs of Ascarislumbricoides in Tangerang City, Banten Province.

scholarly journals Aktivitas antibakteri metabolit sekunder lalat rumah Musca domestica terhadap Staphylococcus aureus ATCC 25923

2020 ◽  
Vol 20 (3) ◽  
Author(s):  
Sang Sanggita Surya ◽  
Farida Juliantina Rachmawaty
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Musca Domestica ◽  
Bacterial Growth ◽  
Antibacterial Activities ◽  
Research Use ◽  
House Flies ◽  
Experimental Laboratory

Abstrak. Lalat merupakan serangga yang dapat membawa berbagai bakteri, salah satunya Staphylococcus aureus. Bakteri tersebut dapat menyebabkan penyakit pada manusia. Hadits Nabi Muhammad s.a.w disebutkan bahwa lalat memiliki penyakit pada satu sisi sayapnya dan penawar pada sisi sayap yang lain. Hasil penelitian ini diharapkan dapat mengetahui gambaran hadits tersebut dan peran metabolit sekunder lalat rumah terhadap pertumbuhan Staphylococcus aureus. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri metabolit sekunder lalat rumah terhadap Staphylococcus aureus serta mengetahui konsentrasi bakteri yang dapat dihambat oleh metabolit sekunder tersebut. Metode penelitian yang digunakan adalah eksperimental laboratorium dengan menguji aktivitas antibakteri metabolit sekunder lalat rumah yang diambil pada menit ke-20 terhadap Staphylococcus aureus yang terbagi menjadi 6 konsentrasi yaitu 101 CFU/ml hingga 106 CFU/ml. Hasil dianalisis secara deskriptif dengan membandingkan pertumbuhan bakteri pada uji metabolit sekunder dengan kontrol. Hasil pengujian didapatkan terdapat hambatan pertumbuhan Staphylococcus aureus pada uji metabolit sekunder lalat rumah dibandingkan dengan kontrol. Konsentrasi yang dapat dihambat sepenuhnya adalah 101 CFU/ml, sedangkan konsentrasi 102 CFU/ml hingga 106 CFU/ml dihambat sebagian. Namun hasil yang diperoleh belum konsisten. Metabolit sekunder lalat rumah memiliki aktivitas antibakteri terhadap Staphylococcus aureus yaitu ditunjukkan oleh hambatan pertumbuhan bakteri pada semua konsentrasi yang diuji Kata Kunci: Aktivitas Antibakteri, Metabolit           Sekunder, Musca   domestica, Staphylococcus aureus Abstract. Flies are insects carry a variety of bacteria, one of it is Staphylococcus aureus. This bacterium cause disease in humans. However, in the hadith of the Prophet Muhammad s.a.w, mentioned that a fly carries a disease on one side of its wings and an antidote on the other side. Therefore, the results of this study are expected to be able to explain the relation with the meaning of the hadith of the Prophet Muhammad s.a.w. and to show the role of secondary metabolites of houseflies on the growth of Staphylococcus aureus. This research is to determine the antibacterial activities of the housefly’s secondary metabolites towards Staphylococcus aureus and the concentration of bacteria that can be inhibited by these secondary metabolites. This research use experimental laboratory which tested the antibacterial activities of the housefly’s secondary metabolites taken in the 20th minute towards Staphylococcus aureus, was divided into six concentrations of 101 CFU/ml to 106 CFU/ml compared with controls. The results were analyzed descriptively by comparing bacterial growth in secondary metabolites test with bacterial growth in control. This research found there is Staphylococcus aureus growth inhibition in the test given secondary metabolites of house flies compared with controls. Concentrations of bacteria that can be inhibited entirely are 101 CFU/ml, while those that can be partially inhibited are at concentrations of 102 CFU/ml to 106 CFU/ml. But the results obtained have not consistent. Secondary metabolites of house flies have antibacterial activity towards Staphylococcus aureus. This is indicated by the inhibition of bacterial growth at all concentrations tested.Keywords: Antibacterial activity, secondary metabolites, Musca domestica, Staphylococcus aureus

Efektifitas Ekstrak Daun Sirih Hijau (Piper Betle) sebagai Insektisida Nabati terhadap Mortalitas Lalat Rumah (Musca domestica)

2019 ◽  
Vol 10 (2) ◽  
pp. 44-50
Author(s):  
Rinaldi Daswito ◽  
Rima Folentia ◽  
M Yusuf MF
Keyword(s):  
Musca Domestica ◽  
Leaf Extract ◽  
Effective Concentration ◽  
P Value ◽  
Piper Betle ◽  
Microbiology Laboratory ◽  
House Flies ◽  
Betel Leaf ◽  
The One ◽  
One Way Anova

One of the diseases that can be transmitted by flies is diarrhea. Green betel leaf contains essential oils, chavicol, arecoline, phenol, and tannins which function as plant-based insecticides. This study aimed to determine the effectiveness of green betel leaf extract (Piper betel) as a plant-based insecticide on the number of mortality of house flies (Musca domestica). The research was an experimental study used After Only Design used the One Way Anova test with a 95% confidence level. The samples used were 360 ​​house flies. Each treatment of 30 house flies with 4 repetitions and used three concentrations of green betel leaf extract (25%, 50%, 75%). The study was conducted at the Chemistry and Microbiology Laboratory of Health Polytechnic Tanjungpinang, while the location of the fly collection was at the Tokojo Garbage Collection Station in Bintan Regency. The number of mortality of house flies at a concentration of 25% was 81 heads (67.5%), 50% concentrations were 93 heads (77.5%), and at a concentration of 75% were 103 heads (85.83%). There was an effect of green betel leaf extract on the mortality of house flies (p-value 0.0001 <0.05) with the most effective concentration of 75%. Further research is needed to obtain a finished product utilizing green betel leaf extract as a vegetable insecticide, especially in controlling the fly vector. Need further research on the use of green betel leaf extract as a vegetable insecticide controlling the fly vector by taking into account the amount of spraying and the age of the fly.   Keywords: Green betel leaf extract , organic insecticide, houseflies

scholarly journals Group size and aquatic vegetation modulates male preferences for female shoals in wild zebrafish, Danio rerio

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Aditya Ghoshal ◽  
Anuradha Bhat
Keyword(s):  
Group Size ◽  
Aquatic Vegetation ◽  
Natural Habitat ◽  
Ecological Factors ◽  
Choice Test ◽  
Female Group ◽  
Aquatic Flora ◽  
In The Wild ◽  
The Individual

AbstractShoaling decisions in the wild are determined by a combination of innate preferences of the individual along with the interplay of multiple ecological factors. In their natural habitat as well as in the laboratory, zebrafish is a shoaling fish. Here, we investigate the role of group size and associated vegetation in shaping shoaling preferences of wild male zebrafish. We studied the association preference of males to groups of female shoals in a multi-choice test design. We found that males made greater proportion of visits to an 8-female group compared to 2 and 4-female groups. However, males spent similar proportions of time across the three female-containing groups. When artificial vegetation was incorporated along with female number as an additional factor, we found that males prefer high and moderately vegetated patches compared to low or no-vegetation groups, irrespective of the number of females in these patches. Based on experiments using a novel multi-choice design, our results show that preference for group size can change due to interaction of two separate factors. This work is a first attempt to understand the role of aquatic flora in determining shoaling preferences in zebrafish, using an experimental paradigm consisting of a gradation in female and vegetation densities.

CONTROL OF EGG FERTILIZATION BY NASONIA VITRIPENNIS (HYMENOPTERA: PTEROMALIDAE) WHEN LAYING ON PARASITIZED HOUSE FLY PUPAE

1973 ◽  
Vol 105 (5) ◽  
pp. 709-718 ◽  
Author(s):  
H. G. Wylie
Keyword(s):  
Musca Domestica ◽  
House Fly ◽  
Nasonia Vitripennis ◽  
House Flies

AbstractFemales of Nasonia vitripennis (Walk.) lay a smaller percentage of fertilized (i.e. female) eggs on house fly, Musca domestica L., pupae previously parasitized by their own species, by Muscidifurax zaraptor K. & L., or by Spalangia cameroni Perk. (Hymenoptera: Pteromalidae) than on unparasitized hosts. They respond to changes in the fly pupae associated with death, and in the case of house flies attacked by N. vitripennis, to "venoms" injected at that time or to changes unrelated to death. By not fertilizing eggs that they lay on attacked hosts, the females also conserve sperm, for immature N. vitripennis on previously-attacked fly pupae are usually killed by parasite larvae already present.

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