Prolonged Viability of Senecavirus A in Exposed House Flies (Musca domestica)

House flies (Musca domestica) are often present in swine farms worldwide. These flies utilize animal secretions and waste as a food source. House flies may harbor and transport microbes and pathogens acting as mechanical vectors for diseases. Senecavirus A (SVA) infection in pigs occurs via oronasal route, and animals shed high virus titers to the environment. Additionally, SVA possesses increased environmental resistance. Due to these reasons, we investigated the tenacity of SVA in house flies. Five groups of flies, each composed of ten females and ten males, were exposed to SVA, titer of 109.3 tissue culture infectious dose (TCID50/mL). Groups of male and female flies were collected at 0, 6, 12, 24, and 48 h post-exposure. For comparison purposes, groups of flies were exposed to Swinepox virus (SwPV). Infectious SVA was identified in all tested groups. Successful isolation of SVA demonstrated the titers varied between 106.8 and 102.8 TCID50/mL in female groups and varied from 105.85 to 103.8 TCID50/mL in male groups. In contrast, infectious SwPV was only detected in the female group at 6 h. The significant SVA infectious titer for prolonged periods of time, up to 48 h, indicates a potential role of flies in SVA transmission.

The Role of Literary Artists in Environmental Movements: Minamata Disease and Michiko Ishimure

By offering new fantasies, perspectives and representations, artists have the power to make people aware of social issues and inspire them to action. This paper describes how artists can offer a vision of environmental resistance by employing fantasy and using tools of poetic expression for communities affected by environmental destruction. This paper employs a case study methodology to examine the Minamata disease victims’ movement in Japan through the lens of environmental justice. As part of this movement, writer Michiko Ishimure created a fantasy called Mouhitotsu-no-konoyo, based in a mythical world and featuring the moral relationships that the people of Minamata, Kumamoto Prefecture, had embraced before modernisation. I will show the importance of this fantasy for the movement, analysing it from two perspectives: those of ningenteki-dori (the human principle) and the invisible fantasy about the mythical world. Ishimure’s fantasy offers a moral message to prevent further environmental harm.

Investigation of Potential Reservoirs of Non-Tuberculous Mycobacteria in a European Sea Bass (Dicentrarchus labrax) Farm

Fish mycobacteriosis is a widespread global problem caused by species of non-tuberculous mycobacteria (NTM). Mycobacterium marinum is one of the species most often involved in disease episodes of aquarium and farmed fish. Since there is currently no available effective therapy or vaccine, a prompt search for routes of entry is key to limiting the damage induced by the disease. Here we report a case of mycobacteriosis follow up in a European sea bass (Dicentrarchus labrax) farm located in Northern Italy, in which environmental samples and newly added fish batches were analyzed. Samples from fish present on the farm, sediment, and periphyton all resulted positive for M. marinum, whereas the new fish batches and the water samples resulted negative. The environmental resistance of NTM (alcohol-acid resistance, biofilm formation) and the lack of prophylactic and therapeutic strategies make these diseases difficult to manage. Prompt identification of biotic and abiotic reservoirs, combined with good zootechnical hygiene practices, are the most effective measures to control fish mycobacteriosis in intensive farms.

Aggressive Environment Resistance of Concrete Products Modified With Nano Alumina and Nano Silica

The service life of concrete products with exposure to an aggressive environment has raised great concerns in the past decades. Nanomaterials have been used as a promising approach to improve the environmental resistance of concrete products when exposed to synergistic attacks. The impacts of CaCl2 on nano-modified concrete, especially along with freeze/thaw (F/T) and wet/dry (W/D) cycles, were barely discussed. In this study, the impacts of CaCl2 along with F/T and W/D cycles on the nano SiO2 and Al2O3 modified concrete were investigated. The mass loss, flexural strength, compressive strength, and relative dynamic modulus of elasticity were tested to evaluate the durability of concrete products. The testing results indicate that the addition of nanoparticles has a distinctive effect on the environment resistance enhancement of concrete samples. The microstructure analysis demonstrates that with the addition of nanoparticles, high-density hydration products were formed, which is beneficial to the properties enhancement of concrete products. This study not only provides an approach to realize the nano modification on the durability of concrete products but also helps to design and fabricate environmentally resistant concrete products when exposed to a synergistic aggressive environment.

The Collimated Propagation Causes of Astrophysical and Laboratory Jets

The use of Z-pinch facilities makes it possible to carry out well-controlled and diagnosable laboratory experiments to study laboratory jets with scaling parameters close to those of the jets from young stars. This makes it possible to observe processes that are inaccessible to astronomical observations. Such experiments are carried out at the PF-3 facility (“plasma focus,” Kurchatov Institute), in which the emitted plasma emission propagates along the drift chamber through the environment at a distance of one meter. The paper presents the results of experiments with helium, in which a successive release of two ejections was observed. An analysis of these results suggests that after the passage of the first supersonic ejection, a region with a low concentration is formed behind it, the so-called vacuum trace, due to which the subsequent ejection practically does not experience environmental resistance and propagates being collimated. The numerical modeling of the propagation of two ejections presented in the paper confirms this point of view. Using scaling laws and appropriate numerical simulations of astrophysical ejections, it is shown that this effect can also be significant for the jets of young stars.

Triazole-Resistance in Environmental Aspergillus fumigatus in Latin American and African Countries

Triazole-resistance has been reported increasingly in Aspergillus fumigatus. An international expert team proposed to avoid triazole monotherapy for the initial treatment of invasive aspergillosis in regions with >10% environmental-resistance, but this prevalence is largely unknown for most American and African countries. Here, we screened 584 environmental samples (soil) from urban and rural locations in Mexico, Paraguay, and Peru in Latin America and Benin and Nigeria in Africa for triazole-resistant A. fumigatus. Samples were screened using triazole-containing agars and confirmed as triazole-resistant by the European Committee on Antimicrobial Susceptibility Testing (EUCAST) broth dilution reference method. Isolates were further characterized by cyp51A sequencing and short-tandem repeat typing. Fungicide presence in samples was likewise determined. Among A. fumigatus positive samples, triazole-resistance was detected in 6.9% (7/102) of samples in Mexico, 8.3% (3/36) in Paraguay, 9.8% (6/61) in Peru, 2.2% (1/46) in Nigeria, and none in Benin. Cyp51A gene mutations were present in most of the triazole-resistant isolates (88%; 15/17). The environmentally-associated mutations TR34/L98H and TR46/Y121F/T289A were prevalent in Mexico and Peru, and isolates harboring these mutations were closely related. For the first time, triazole-resistant A. fumigatus was found in environmental samples in Mexico, Paraguay, Peru, and Nigeria with a prevalence of 7–10% in the Latin American countries. Our findings emphasize the need to establish triazole-resistance surveillance programs in these countries.

Biofilm and Spore Formation of Clostridium perfringens and Its Resistance to Disinfectant and Oxidative Stress

Clostridium perfringens is a major human pathogen that causes gastroenteritis via enterotoxin production and has the ability to form spores and biofilms for environmental persistence and disease transmission. This study aimed to compare the disinfectant and environmental resistance properties of C. perfringens vegetative cells and spores in planktonic and sessile conditions, and to examine the nucleotide polymorphisms and transcription under sessile conditions in C. perfringens strains isolated from meat. The sporulation rate of sessile C. perfringens TYJAM-D-66 (cpe+) was approximately 19% at day 5, while those of CMM-C-80 (cpe-) and SDE-B-202 (cpe+) were only 0.26% and 0.67%, respectively, at day 7. When exposed to aerobic conditions for 36 h, TYJAM-D-66, CMM-C-80, and SDE-B-202 vegetative cells showed 1.70 log, 5.36 log, and 5.67 log reductions, respectively. After treatment with sodium hypochlorite, the survival rates of TYJAM-D-66 vegetative cells (53.6%) and spores (82.3%) in biofilms were higher than those of planktonic cells (9.23%). Biofilm- and spore-related genes showed different expression within TYJAM-D-66 (–4.66~113.5), CMM-C-80 (–3.02~2.49), and SDE-B-202 (–5.07~2.73). Our results indicate the resistance of sessile cells and spores of C. perfringens upon exposure to stress conditions after biofilm formation.

Role of Phage Capsid in the Resistance to UV-C Radiations

The conformational variation of the viral capsid structure plays an essential role both for the environmental resistance and acid nuclear release during cellular infection. The aim of this study was to evaluate how capsid rearrangement in engineered phages of M13 protects viral DNA and peptide bonds from damage induced by UV-C radiation. From in silico 3D modelling analysis, two M13 engineered phage clones, namely P9b and 12III1, were chosen for (i) chemical features of amino acids sequences, (ii) rearrangements in the secondary structure of their pVIII proteins and (iii) in turn the interactions involved in phage capsid. Then, their resistance to UV-C radiation and hydrogen peroxide (H2O2) was compared to M13 wild-type vector (pC89) without peptide insert. Results showed that both the phage clones acquired an advantage against direct radiation damage, due to a reorganization of interactions in the capsid for an increase of H-bond and steric interactions. However, only P9b had an increase in resistance against H2O2. These results could help to understand the molecular mechanisms involved in the stability of new virus variants, also providing quick and necessary information to develop effective protocols in the virus inactivation for human activities, such as safety foods and animal-derived materials.