scholarly journals Proteomic and Bioinformatic Analysis of Streptococcus suis Human Isolates: Combined Prediction of Potential Vaccine Candidates

Vaccines ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 188 ◽  
Author(s):  
Esther Prados de la Torre ◽  
Antonio Rodríguez-Franco ◽  
Manuel J. Rodríguez-Ortega
Keyword(s):  
Streptococcus Suis ◽  
Bioinformatic Analysis ◽  
Surface Proteins ◽  
Clinical Isolates ◽  
Economic Losses ◽  
Bacterial Cells ◽  
Label Free ◽  
Bioinformatic Tools ◽  
Potential Vaccine ◽  
Live Bacterial Cells

Streptococcus suis is a Gram-positive bacterium responsible for major infections in pigs and economic losses in the livestock industry, but also an emerging zoonotic pathogen causing serious diseases in humans. No vaccine is available so far against this microorganism. Conserved surface proteins are among the most promising candidates for new and effective vaccines. Until now, research on this pathogen has focused on swine isolates, but there is a lack of studies to identify and characterize surface proteins from human clinical isolates. In this work, we performed a comparative proteomic analysis of six clinical isolates from human patients, all belonging to the major serotype 2, by “shaving” the live bacterial cells with trypsin, followed by LC-MS/MS analysis. We identified 131 predicted surface proteins and carried out a label-free semi-quantitative analysis of protein abundances within the six strains. Then, we combined our proteomics results with bioinformatic tools to help improving the selection of novel antigens that can enter the pipeline of vaccine candidate testing. Our work is then a complement to the reverse vaccinology concept.

scholarly journals Comparative Exoproteome Analysis of Streptococcus suis Human Isolates

2021 ◽  
Vol 9 (6) ◽  
pp. 1287
Author(s):  
Esther Prados de la Torre ◽  
Antonio Rodríguez-Franco ◽  
Manuel J. Rodríguez-Ortega
Keyword(s):  
Animal Health ◽  
Streptococcus Suis ◽  
Bioinformatic Analysis ◽  
Infection Process ◽  
Surface Proteins ◽  
Secreted Proteins ◽  
Extracellular Milieu ◽  
Cytoplasmic Proteins ◽  
Swine Pathogen ◽  
Predicted Signal Peptide

The swine pathogen Streptococcus suis is a Gram-positive bacterium which causes infections in pigs, with an impact in animal health and in the livestock industry, and it is also an important zoonotic agent. During the infection process, surface and secreted proteins are essential in the interaction between microorganisms and their hosts. Here, we report a comparative proteomic analysis of the proteins released to the extracellular milieu in six human clinical isolates belonging to the highly prevalent and virulent serotype 2. The total secreted content was precipitated and analyzed by GeLC-MS/MS. In the six strains, 144 proteins assigned to each of the categories of extracellular or surface proteins were identified, as well as 680 predicted cytoplasmic proteins, many of which are putative moonlighting proteins. Of the nine predicted signal peptide-I secreted proteins, seven had relevant antigenic potential when they were analyzed through bioinformatic analysis. This is the first work comparing the exoproteome fraction of several human isolates of this important pathogen.

scholarly journals Search of Potential Vaccine Candidates against Trueperella pyogenes Infections through Proteomic and Bioinformatic Analysis

Vaccines ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 314 ◽  
Author(s):  
Ángela Galán-Relaño ◽  
Lidia Gómez-Gascón ◽  
Antonio Rodríguez-Franco ◽  
Inmaculada Luque ◽  
Belén Huerta ◽  
...  
Keyword(s):  
Cell Wall ◽  
Membrane Proteins ◽  
Bioinformatic Analysis ◽  
Surface Proteins ◽  
Live Cells ◽  
Economic Losses ◽  
Diagnostic Tools ◽  
Cell Wall Proteins ◽  
Vaccine Candidates ◽  
Trueperella Pyogenes

Trueperella pyogenes is an opportunistic pathogen, responsible for important infections in pigs and significant economic losses in swine production. To date, there are no available commercial vaccines to control diseases caused by this bacterium. In this work, we performed a comparative proteomic analysis of 15 T. pyogenes clinical isolates, by “shaving” live cells, followed by LC-MS/MS, aiming at the identification of the whole set of surface proteins (i.e., the “pan-surfome”) as a source of antigens to be tested in further studies as putative vaccine candidates, or used in diagnostic tools. A total of 140 surface proteins were detected, comprising 25 cell wall proteins, 10 secreted proteins, 23 lipoproteins and 82 membrane proteins. After describing the “pan-surfome”, the identified proteins were ranked in three different groups based on the following criteria: to be (i) surface-exposed, (ii) highly conserved and (iii) widely distributed among different isolates. Two cell wall proteins, three lipoproteins, four secreted and seven membrane proteins were identified in more than 70% of the studied strains, were highly expressed and highly conserved. These proteins are potential candidates, alone or in combination, to obtain effective vaccines against T. pyogenes or to be used in the diagnosis of this pathogen.

Real-time monitoring and inhibition of the activity of carbapenemase in live bacterial cells: application to screening of β-lactamase inhibitors

2020 ◽  
Vol 44 (46) ◽  
pp. 20334-20340
Author(s):  
Han Gao ◽  
Ying Ge ◽  
Min-Hao Jiang ◽  
Cheng Chen ◽  
Le-Yun Sun ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Real Time ◽  
Bacterial Cells ◽  
Real Time Monitoring ◽  
Live Bacterial Cells

Antibiotic resistance mediated by β-lactamases including metallo-β-lactamases (MβLs) has become an emerging threat.

scholarly journals Streptococcus suis Induces Expression of Cyclooxygenase-2 in Porcine Lung Tissue

2021 ◽  
Vol 9 (2) ◽  
pp. 366
Author(s):  
Muriel Dresen ◽  
Josephine Schenk ◽  
Yenehiwot Berhanu Weldearegay ◽  
Désirée Vötsch ◽  
Wolfgang Baumgärtner ◽  
...  
Keyword(s):  
Lung Tissue ◽  
Streptococcus Suis ◽  
Cyclooxygenase 2 ◽  
Economic Losses ◽  
Biological Processes ◽  
Alveolar Tissue ◽  
Cox 2 ◽  
Ciliated Epithelial Cells ◽  
Immunohistological Analysis

Streptococcus suis is a common pathogen colonising the respiratory tract of pigs. It can cause meningitis, sepsis and pneumonia leading to economic losses in the pig industry worldwide. Cyclooxygenase-2 (COX-2) and its metabolites play an important regulatory role in different biological processes like inflammation modulation and immune activation. In this report we analysed the induction of COX-2 and the production of its metabolite prostaglandin E2 (PGE2) in a porcine precision-cut lung slice (PCLS) model. Using Western blot analysis, we found a time-dependent induction of COX-2 in the infected tissue resulting in increased PGE2 levels. Immunohistological analysis revealed a strong COX-2 expression in the proximity of the bronchioles between the ciliated epithelial cells and the adjacent alveolar tissue. The morphology, location and vimentin staining suggested that these cells are subepithelial bronchial fibroblasts. Furthermore, we showed that COX-2 expression as well as PGE2 production was detected following infection with two prevalent S. suis serotypes and that the pore-forming toxin suilysin played an important role in this process. Therefore, this study provides new insights in the response of porcine lung cells to S. suis infections and serves as a basis for further studies to define the role of COX-2 and its metabolites in the inflammatory response in porcine lung tissue during infections with S. suis.

scholarly journals Controllable gold-capped nanoporous anodic alumina chip for label-free, specific detection of bacterial cells

RSC Advances ◽  
2017 ◽  
Vol 7 (30) ◽  
pp. 18815-18820 ◽  
Author(s):  
Do-Kyun Kim ◽  
Dong Min Kim ◽  
Seung Min Yoo ◽  
Sang Yup Lee
Keyword(s):  
Anodic Alumina ◽  
Bacterial Cells ◽  
Label Free ◽  
Single Chip ◽  
Specific Detection ◽  
Nanoporous Anodic Alumina ◽  
Quantitative Manner

A gold-capped nanostructured PAA sensor that uses aptamers detected bacterial cells in a quantitative manner with high specificities on a single chip.

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