scholarly journals Ascomycetes versus Spent Mushroom Substrate in Mycoremediation of Dredged Sediments Contaminated by Total Petroleum Hydrocarbons: The Involvement of the Bacterial Metabolism

Water ◽  
2021 ◽  
Vol 13 (21) ◽  
pp. 3040
Author(s):  
Simone Becarelli ◽  
Giovanna Siracusa ◽  
Ilaria Chicca ◽  
Giacomo Bernabei ◽  
Simona Di Gregorio
Keyword(s):  
Petroleum Hydrocarbons ◽  
Bacterial Species ◽  
Substrate Inhibition ◽  
Diesel Oil ◽  
Total Petroleum Hydrocarbons ◽  
Bacterial Metabolism ◽  
Spent Mushroom Substrate ◽  
Dredged Sediments ◽  
Prediction Approach ◽  
Kinetics Of

Two mycoremediation approaches for the depletion of the total petroleum hydrocarbons in dredged sediments were compared: co-composting with spent mushroom substrate (SMS) from Pleurotus ostreatus and bioaugmentation with Lambertella sp. MUT 5852, an ascomycetes autochthonous to the sediment, capable of utilizing diesel oil its sole carbon source. After 28 days of incubation, 99% depletion was observed in presence of Lambertella sp. MUT 5852. No total petroleum hydrocarbon depletion was observed in sediment co-composting with the SMS after 60 days of incubation. 16S rDNA metabarcoding of the bacterial community was performed to evaluate the potential synergism between fungi and bacteria in the bioremediation process. The functional metagenomic prediction approach indicated that the biodiversity of the bacterial genera potentially involved in the degradation of TPH was higher in sediment bioaugmented with Lambertella sp. MUT 5852, which resulted in being mandatory for TPH depletion. Mechanisms of co-substrate inhibition of the hydrocarburoclastic bacterial species, due to the bioavailable organic matter of the SMS, are suggested to be involved in the observed kinetics of TPH depletion, failing in the case of SMS and successful in the case of Lambertella sp. MUT 5852.

scholarly journals A New Ciboria sp. for Soil Mycoremediation and the Bacterial Contribution to the Depletion of Total Petroleum Hydrocarbons

2021 ◽  
Vol 12 ◽  
Author(s):  
Simone Becarelli ◽  
Ilaria Chicca ◽  
Salvatore La China ◽  
Giovanna Siracusa ◽  
Alessandra Bardi ◽  
...  
Keyword(s):  
Petroleum Hydrocarbons ◽  
Sole Carbon Source ◽  
Bacterial Species ◽  
Diesel Oil ◽  
Polluted Soil ◽  
Fungal Strain ◽  
Oil Refinery ◽  
Fungal Communities ◽  
Total Petroleum Hydrocarbons ◽  
Fresh Weight

A Ciboria sp. strain (Phylum Ascomycota) was isolated from hydrocarbon-polluted soil of an abandoned oil refinery in Italy. The strain was able to utilize diesel oil as a sole carbon source for growth. Laboratory-scale experiments were designed to evaluate the use of this fungal strain for treatment of the polluted soil. The concentration of total petroleum hydrocarbons (TPH) in the soil was 8,538 mg/kg. Mesocosms containing the contaminated soil were inoculated with the fungal strain at 1 or 7%, on a fresh weight base ratio. After 90 days of incubation, the depletion of TPH contamination was of 78% with the 1% inoculant, and 99% with the 7% inoculant. 16S rDNA and ITS metabarcoding of the bacterial and fungal communities was performed in order to evaluate the potential synergism between fungi and bacteria in the bioremediation process. The functional metagenomic prediction indicated Arthrobacter, Dietzia, Brachybacerium, Brevibacterium, Gordonia, Leucobacter, Lysobacter, and Agrobacterium spp. as generalist saprophytes, essential for the onset of hydrocarbonoclastic specialist bacterial species, identified as Streptomyces, Nocardoides, Pseudonocardia, Solirubrobacter, Parvibaculum, Rhodanobacter, Luteiomonas, Planomicrobium, and Bacillus spp., involved in the TPH depletion. The fungal metabolism accelerated the onset of specialist over generalist bacteria. The capacity of the Ciboria sp. to deplete TPH in the soil in treatment was also ascertained.

Efficiency and Kinetics of Total Petroleum Hydrocarbons (TPHs) Removal from Crude Oil Polluted Arable Soil using Palm Bunch Ash and Tween 80

2021 ◽  
Author(s):  
Godwin James Udo ◽  
Nnanake-Abasi O. Offiong ◽  
Alfreda Nwadinigwe ◽  
Clement O. Obadimu ◽  
Aniedi E. Nyong ◽  
...  
Keyword(s):  
Crude Oil ◽  
Petroleum Hydrocarbons ◽  
Tween 80 ◽  
Arable Soil ◽  
Total Petroleum Hydrocarbons ◽  
Kinetics Of ◽  
Palm Bunch

scholarly journals The use of vinasse as an amendment to ex-situ bioremediation of soil and groundwater contaminated with diesel oil

2009 ◽  
Vol 52 (4) ◽  
pp. 1043-1055 ◽  
Author(s):  
Adriano Pinto Mariano ◽  
Sérgio Henrique Rezende Crivelaro ◽  
Dejanira de Franceschi de Angelis ◽  
Daniel Marcos Bonotto
Keyword(s):  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Microbial Population ◽  
Diesel Oil ◽  
Ex Situ ◽  
Total Petroleum Hydrocarbons ◽  
Co2 Production ◽  
Soil Bioremediation ◽  
Negative Effects ◽  
Labile Carbon

This work investigated the possibility of using vinasse as an amendment in ex-situ bioremediation processes. Groundwater and soil samples were collected at petrol stations. The soil bioremediation was simulated in Bartha biometer flasks, used to measure the microbial CO2 production, during 48 days, where vinasse was added at a concentration of 33 mL.Kg-1of soil. Biodegradation efficiency was also measured by quantifying the total petroleum hydrocarbons (TPH) by gas chromatography. The groundwater bioremediation was carried out in laboratory experiments simulating aerated (bioreactors) and not aerated (BOD flasks) conditions. In both the cases, the concentration of vinasse was 5 % (v/v) and different physicochemical parameters were evaluated during 20 days. Although an increase in the soil fertility and microbial population were obtained with the vinasse, it demonstrated not to be adequate to enhance the bioremediation efficiency of diesel oil contaminated soils. The addition of the vinasse in the contaminated groundwaters had negative effects on the biodegradation of the hydrocarbons, since vinasse, as a labile carbon source, was preferentially consumed.

Composting Study of Petroleum Contaminated Soil

2013 ◽  
Vol 864-867 ◽  
pp. 67-70
Author(s):  
Xue Ying Song ◽  
Ru Jing Liang ◽  
Yu Shuang Li ◽  
Xin Xin Li ◽  
Xiao Jun Hu
Keyword(s):  
Contaminated Soil ◽  
Petroleum Hydrocarbons ◽  
Organic Amendments ◽  
Dry Weight ◽  
Diesel Oil ◽  
Weight Basis ◽  
Total Petroleum Hydrocarbons ◽  
Major Objective ◽  
Degradation Rates ◽  
Petroleum Contaminated Soil

Composting has been shown to be an effective bioremediation technique for the treatment of hydrocarbon-contaminated soil. In this research, the major objective of this research was to find the appropriate mix ratio of organic amendments for enhancing the degradation of petroleum hydrocarbons during diesel oil contaminated soil composting. The spent mushroom was added as an amendment for supplementing organic matter for composting of contaminated soil. The volumn ratios of contaminated soil to organic amendments were 1:1, 1.5:1 and 2:1. Target contaminant of this research was diesel oil, which was spiked at 16240 mg/kg sample on a dry weight basis. The degradation of diesel oil was significantly enhanced by the addition of these organic amendments relative to straight soil control. Degradation rates of total petroleum hydrocarbons (TPH) were the greatest at the ratio of 1:1 of contaminated soil to organic amendments on the volumn ratio. The abiotic loss of TPH was only about 6.83% of initial TPH.

Optimization of diesel oil biodegradation in seawater using statistical experimental methodology

2012 ◽  
Vol 66 (6) ◽  
pp. 1301-1309 ◽  
Author(s):  
Wenxiang Xia ◽  
Jincheng Li ◽  
Yan Xia ◽  
Zhiwen Song ◽  
Jihong Zhou
Keyword(s):  
Response Surface ◽  
Petroleum Hydrocarbons ◽  
Diesel Oil ◽  
Experimental Methodology ◽  
Total Petroleum Hydrocarbons ◽  
Quadratic Model ◽  
Phosphorus Concentration ◽  
Oil Degradation ◽  
Nitrogen And Phosphorus ◽  
Oil Biodegradation

Petroleum hydrocarbons released into the environment can be harmful to higher organisms, but they can be utilized by microorganisms as the sole source of energy for metabolism. To investigate the optimal conditions of diesel oil biodegradation, the Plackett–Burman (PB) design was used for the optimization in the first step, and N source (NaNO3), P source (KH2PO4) and pH were found to be significant factors affecting oil degradation. Then the response surface methodology (RSM) using a central composite design (CCD) was adopted for the augmentation of diesel oil biodegradation and a fitted quadratic model was obtained. The model F-value of 27.25 and the low probability value (<0.0001) indicate that the model is significant and that the concentration of NaNO3N, KH2PO4 and pH had significant effects on oil removal during the study. Three-dimensional response surface plots were constructed by plotting the response (oil degradation efficiency) on the z-axis against any two independent variables, and the optimal biodegradation conditions of diesel oil (original total petroleum hydrocarbons 125 mg/L) were determined as follows: NaNO3 0.143 g, KH2PO4 0.022 g and pH 7.4. These results fit quite well with the C, N and P ratio in biological cells. Results from the present study might provide a new method to estimate the optimal nitrogen and phosphorus concentration in advance for oil biodegradation according to the composition of petroleum.

Total Petroleum Hydrocarbons Categories Concentration in Soils Within the Vicinity Housing Heavy-Duty Diesel Generators in Three Universities in Port Harcourt South-South Nigeria

2021 ◽  
Vol 4 (1) ◽  
Keyword(s):  
Petroleum Hydrocarbons ◽  
Health Hazard ◽  
Soxhlet Extraction ◽  
Soil Samples ◽  
Total Petroleum Hydrocarbons ◽  
Heavy Duty ◽  
Flame Ionization ◽  
Port Harcourt ◽  
Diesel Generators ◽  
Heavy Duty Diesel

Total petroleum hydrocarbons pollution of soil samples randomly collected from three Nigeria Universities in Port Harcourt due to the use of heavy-duty diesel generators was studied to ascertains the level of concentration of the different hydrocarbons’ categories. The soil samples were collected at two different depths of 0.00-0.50m and 0.50-1.00m. The Universities were Ignatius Ajuru University of Education (IAUE), Rivers State University (RSU) and University of Port Harcourt (UNIPORT). The different total petroleum hydrocarbons categories were Gasoline Range Organics (GRO), Diesel Range Organics (DRO) and Lube Oil Range. Soxhlet extraction method was used in extracting the samples and due column clean-up was performed for chromatographic analysis. Gas Chromatography-Flame Ionization Detector was used to determine the level of concentrations of the different categories of total petroleum hydrocarbons. The results showed that at 0.00-0.50m depth, IAUE was 4.42145, 945.4784, and 525.66919 mg/Kg for GRO, DRO and lube oil range respectively, RSU was not detected, 494.44799 and 458.6715 mg/Kg for GRO, DRO and lube oil range respectively and UNIPORT was 4.40920, 501.2246 and 467.71426 mg/Kg for GRO, DRO and lube oil range respectively. At 0.50-1.00m depth IAUE was 2.75132, 596.35126, and 311.84451 mg/Kg for GRO, DRO and lube oil range respectively, RSU was not detected, 298.06899 and 270.61619 mg/Kg for GRO, DRO and lube oil range respectively and UNIPORT was 2.77780, 301.74701 and 276.88684 mg/Kg for GRO, DRO and lube oil range respectively. The level of soil contamination Showed that GRO > DRO > lube oil range. The observation showed that hydrocarbon pollution decreased with increase in depth. The level of DRO and lube oil range in the studied areas exceeded the limit acceptable and therefore adequate steps should be taken to remedy the situation so that it will not pose any health hazard to the workers operating the heavy-duty generators.

Fate of Total Petroleum Hydrocarbons in the Environment

2019 ◽  
pp. 57-77 ◽  
Author(s):  
Saranya Kuppusamy ◽  
Naga Raju Maddela ◽  
Mallavarapu Megharaj ◽  
Kadiyala Venkateswarlu
Keyword(s):  
Petroleum Hydrocarbons ◽  
Total Petroleum Hydrocarbons
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