scholarly journals The use of vinasse as an amendment to ex-situ bioremediation of soil and groundwater contaminated with diesel oil

2009 ◽  
Vol 52 (4) ◽  
pp. 1043-1055 ◽  
Author(s):  
Adriano Pinto Mariano ◽  
Sérgio Henrique Rezende Crivelaro ◽  
Dejanira de Franceschi de Angelis ◽  
Daniel Marcos Bonotto
Keyword(s):  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Microbial Population ◽  
Diesel Oil ◽  
Ex Situ ◽  
Total Petroleum Hydrocarbons ◽  
Co2 Production ◽  
Soil Bioremediation ◽  
Negative Effects ◽  
Labile Carbon

This work investigated the possibility of using vinasse as an amendment in ex-situ bioremediation processes. Groundwater and soil samples were collected at petrol stations. The soil bioremediation was simulated in Bartha biometer flasks, used to measure the microbial CO2 production, during 48 days, where vinasse was added at a concentration of 33 mL.Kg-1of soil. Biodegradation efficiency was also measured by quantifying the total petroleum hydrocarbons (TPH) by gas chromatography. The groundwater bioremediation was carried out in laboratory experiments simulating aerated (bioreactors) and not aerated (BOD flasks) conditions. In both the cases, the concentration of vinasse was 5 % (v/v) and different physicochemical parameters were evaluated during 20 days. Although an increase in the soil fertility and microbial population were obtained with the vinasse, it demonstrated not to be adequate to enhance the bioremediation efficiency of diesel oil contaminated soils. The addition of the vinasse in the contaminated groundwaters had negative effects on the biodegradation of the hydrocarbons, since vinasse, as a labile carbon source, was preferentially consumed.

scholarly journals Soil Bioremediation: Overview of Technologies and Trends

Energies ◽  
2020 ◽  
Vol 13 (18) ◽  
pp. 4664
Author(s):  
Israel Gonçalves Sales da Silva ◽  
Fabíola Carolina Gomes de Almeida ◽  
Nathália Maria Padilha da Rocha e Silva ◽  
Alessandro Alberto Casazza ◽  
Attilio Converti ◽  
...  
Keyword(s):  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Ex Situ ◽  
Soil Bioremediation ◽  
Application Of Surfactants ◽  
Agricultural Pesticides ◽  
Advantages And Disadvantages ◽  
Microbiological Characteristics ◽  
Extent Of Damage

Petroleum hydrocarbons, heavy metals and agricultural pesticides have mutagenic, carcinogenic, immunotoxic and teratogenic effects and cause drastic changes in soil physicochemical and microbiological characteristics, thereby representing a serious danger to health and environment. Therefore, soil pollution urgently requires the application of a series of physicochemical and biological techniques and treatments to minimize the extent of damage. Among them, bioremediation has been shown to be an alternative that can offer an economically viable way to restore polluted areas. Due to the difficulty in choosing the best bioremediation technique for each type of pollutant and the paucity of literature on soil bioremediation enhanced by the use of specific additives, we reviewed the main in situ and ex situ methods, their current properties and applications. The first section discusses the characteristics of each class of pollutants in detail, while the second section presents current bioremediation technologies and their main uses, followed by a comparative analysis showing their respective advantages and disadvantages. Finally, we address the application of surfactants and biosurfactants as well as the main trends in the bioremediation of contaminated soils.

scholarly journals Bioremediation of Soil Contaminated with Diesel using Biopile system

2018 ◽  
Vol 14 (3) ◽  
pp. 48-56
Author(s):  
Noor Mohsen Jabbar ◽  
Estabriq Hasan Kadhim ◽  
Alaa Kareem Mohammed
Keyword(s):  
Contaminated Soil ◽  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Bacterial Culture ◽  
Atmospheric Temperature ◽  
Pilot Scale ◽  
Ex Situ ◽  
Total Petroleum Hydrocarbons ◽  
Bacterial Inoculum ◽  
Amended Soil

This study was focused on biotreatment of soil which polluted by petroleum compounds (Diesel) which caused serious environmental problems. One of the most effective and promising ways to treat diesel-contaminated soil is bioremediation. It is a choice that offers the potential to destroy harmful pollutants using biological activity. The capability of mixed bacterial culture was examined to remediate the diesel-contaminated soil in bio piling system. For fast ex-situ treatment of diesel-contaminated soils, the bio pile system was selected. Two pilot scale bio piles (25 kg soil each) were constructed containing soils contaminated with approximately 2140 mg/kg total petroleum hydrocarbons (TPHs). The amended soil: (contaminated soil with the addition of nutrients and bacterial inoculum), where the soil was mixed with 1.5% of sawdust, then supplied with the necessary nutrients and watered daily to provide conditions promoting microorganism growth. Unamended soil was prepared as a control (contaminated soil without addition).  Both systems were equipped with oxygen to provide aerobic conditions, incubated at atmospheric temperature and weekly sampling within 35 days. Overall 75% of the total petroleum hydrocarbons were removed from the amended soil and 38 % of the control soil at the end of study period. The study concluded that ex-situ experiment (Bio pile) is a preferable, economical, and environmentally friendly procedure, thus representing a good option for the treatment of soil contaminated with diesel.

scholarly journals Investigation about the efficiency of the bioaugmentation technique when applied to diesel oil contaminated soils

2009 ◽  
Vol 52 (5) ◽  
pp. 1297-1312 ◽  
Author(s):  
Adriano Pinto Mariano ◽  
Dejanira de Franceschi de Angelis ◽  
Maria Paula Santos Pirôllo ◽  
Jonas Contiero ◽  
Daniel Marcos Bonotto
Keyword(s):  
Contaminated Soils ◽  
Diesel Oil ◽  
Co2 Production ◽  
Negative Effects ◽  
Service Station ◽  
Local Service ◽  
Service Stations ◽  
Redox Indicator ◽  
Biodegradation Efficiency

This work investigated the efficiency of the bioaugmentation technique when applied to diesel oil contaminated soils collected at three service stations. Batch biodegradation experiments were carried out in Bartha biometer flasks (250 mL) used to measure the microbial CO2 production. Biodegradation efficiency was also measured by quantifying the concentration of hydrocarbons. In addition to the biodegradation experiments, the capability of the studied cultures and the native microorganisms to biodegrade the diesel oil purchased from a local service station, was verified using a technique based on the redox indicator 2,6 -dichlorophenol indophenol (DCPIP). Results obtained with this test showed that the inocula used in the biodegradation experiments were able to degrade the diesel oil and the tests carried out with the native microorganisms indicated that these soils had a microbiota adapted to degrade the hydrocarbons. In general, no gain was obtained with the addition of microorganisms or even negative effects were observed in the biodegradation experiments.

scholarly journals Bioremediation of Soil Contaminated with Diesel using Biopile system

2018 ◽  
Vol 14 (3) ◽  
pp. 48-56 ◽  
Author(s):  
Noor Mohsen Jabbar ◽  
Estabriq Hasan Kadhim ◽  
Alaa Kareem Mohammed
Keyword(s):  
Contaminated Soil ◽  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Bacterial Culture ◽  
Atmospheric Temperature ◽  
Pilot Scale ◽  
Ex Situ ◽  
Total Petroleum Hydrocarbons ◽  
Bacterial Inoculum ◽  
Amended Soil

This study was focused on biotreatment of soil which polluted by petroleum compounds (Diesel) which caused serious environmental problems. One of the most effective and promising ways to treat diesel-contaminated soil is bioremediation. It is a choice that offers the potential to destroy harmful pollutants using biological activity. The capability of mixed bacterial culture was examined to remediate the diesel-contaminated soil in bio piling system. For fast ex-situ treatment of diesel-contaminated soils, the bio pile system was selected. Two pilot scale bio piles (25 kg soil each) were constructed containing soils contaminated with approximately 2140 mg/kg total petroleum hydrocarbons (TPHs). The amended soil: (contaminated soil with the addition of nutrients and bacterial inoculum), where the soil was mixed with 1.5% of sawdust, then supplied with the necessary nutrients and watered daily to provide conditions promoting microorganism growth. Unamended soil was prepared as a control (contaminated soil without addition).  Both systems were equipped with oxygen to provide aerobic conditions, incubated at atmospheric temperature and weekly sampling within 35 days. Overall 75% of the total petroleum hydrocarbons were removed from the amended soil and 38 % of the control soil at the end of study period. The study concluded that ex-situ experiment (Bio pile) is a preferable, economical, and environmentally friendly procedure, thus representing a good option for the treatment of soil contaminated with diesel.

Effect of compost in phytoremediation of diesel-contaminated soils

2001 ◽  
Vol 43 (2) ◽  
pp. 291-295 ◽  
Author(s):  
J. Vouillamoz ◽  
M. W. Milke
Keyword(s):  
Moisture Content ◽  
Contaminated Soil ◽  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Dilution Effect ◽  
Screening Tests ◽  
Total Petroleum Hydrocarbons ◽  
Controlled Environment ◽  
Grass Growth ◽  
The Impact

The effect of compost on phytoremediation of diesel-contaminated soils was investigated using 130 small (200 g) containers in two screening tests. The experiments were conducted in a controlled environment using ryegrass from seed. Containers were destructively sampled at various times and analyzed for plant mass and total petroleum hydrocarbons. The results indicate that the presence of diesel reduces grass growth, and that compost helps reduced the impact of diesel on grass growth. The addition of compost helps increase diesel loss from the soils both with and without grass, though the addition of grass leads to lower diesel levels compared with controls. A second set of experiments indicates that the compost helps in phytoremediation of diesel-contaminated soil independent of the dilution effect that compost addition has. The results indicate that the compost addition allowed diesel loss down to 200 mg TPH/kg even though the compost would be expected to hold the diesel more tightly in the soil/compost mixture. The simplicity of the screening tests led to difficulties in controlling moisture content and germination rates. The conclusion of the research is that the tilling of compost into soils combined with grass seeding appears to be a valuable option for treating petroleum-contaminated soils.

scholarly journals Evolution of humic acids during ex situ bioremediation on a pilot level: The added value of the microbial activity

2020 ◽  
Vol 85 (6) ◽  
pp. 821-830
Author(s):  
Aleksandra Zeradjanin ◽  
Jelena Avdalovic ◽  
Marija Ljesevic ◽  
Olivera Tesic ◽  
Srdjan Miletic ◽  
...  
Keyword(s):  
Humic Acids ◽  
Petroleum Hydrocarbons ◽  
Added Value ◽  
Ex Situ ◽  
Total Petroleum Hydrocarbons ◽  
Number Of Microorganisms ◽  
The Fourier Transform ◽  
The Relationship ◽  
Acid Structure ◽  
Qualitative Changes

Environmental pollution is a global problem, while bioremediation technology removes pollutants from the environment using microorganisms. This study was aimed at investigating how a bioremediation process affected soil humification. In soil polluted with petroleum and its derivatives that was submitted to bioremediation, besides the total petroleum hydrocarbons and the number of microorganisms, quantitative and qualitative changes of isolated humic acids were determined during the process. The bioremediation of 150 m3 of polluted soil lasted 150 days. The level of total petroleum hydrocarbons decreased by 86.6 %, while the level of humic acids increased by 26.5 %. The elemental analysis showed the reduction of C and the H/C ratio and the increase of O and the O/C ratio of isolated humic acids during the process. The ratio of absorbencies at 465 and 665 nm also increased. Based on this and the Fourier-transform infrared spectra, it was shown that the humic acids isolated at the end of bioremediation were enriched with oxygen functional groups and aromatic structures. This study provides one of the first insights into the relationship between bioremediation and humification, as well as evidence of how hydrocarbon-degrading microorganisms have a significant influence on changes to humic acid structure during bioremediation.

scholarly journals Biodegradation Potentials of Aspergillus sydowii and Fusarium lichenicola on Total Petroleum Hydrocarbon in an Oilfield Wastewater in Rivers State

2020 ◽  
pp. 1-7
Author(s):  
Williams, Janet Olufunmilayo ◽  
Aleruchi Owhonka
Keyword(s):  
Mixed Culture ◽  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
Total Petroleum Hydrocarbon ◽  
Total Petroleum Hydrocarbons ◽  
Aspergillus Sydowii ◽  
Significant Difference ◽  
Set Up ◽  
Rivers State

This study investigated the potential of Aspergillus sydowii and Fusarium lichenicola as mixed cultures in the biodegradation of Total Petroleum Hydrocarbons TPHs in oilfield wastewater. Oilfield wastewater was collected from an onshore oil producing platform and biodegradation of total petroleum hydrocarbons was investigated using standard methods. Fungi were isolated from oilfield wastewater contaminated soils obtained from the vicinity of the oil producing platform. Experimental control set-up and treatment with mixed culture of fungal isolates were periodically analyzed on days 7 and 21 intervals for total petroleum hydrocarbon degradation using Gas Chromatography (GC). The total amount of TPHs on day 1 recorded 381. 871 mg/l.  The amount of TPHs on days 7 and 21 in the mixed culture of fungi was 108.975 mg/l and 21.105 mg/l respectively while TPHs in control was 342.891 mg/l and 240.749 mg/l respectively. There was a significant difference between the mixed culture and the control on days 7 and 21 at p≤0.05. The results therefore revealed actual and significant reduction of TPHs in the mixed culture. In addition, there was clearance of n-alkanes by the mixed culture. This suggests that fungi have great potentials in biodegradation of TPHs and in remediation of TPH contaminated environments.

Biodegradation of total petroleum hydrocarbons (TPH) in highly contaminated soils by natural attenuation and bioaugmentation

Chemosphere ◽  
2019 ◽  
Vol 234 ◽  
pp. 864-874 ◽  
Author(s):  
Marie Thérèse Bidja Abena ◽  
Tongtong Li ◽  
Muhammad Naeem Shah ◽  
Weihong Zhong
Keyword(s):  
Natural Attenuation ◽  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Total Petroleum Hydrocarbons

scholarly journals Production of CO2 in crude oil bioremediation in clay soil

2005 ◽  
Vol 48 (spe) ◽  
pp. 249-255 ◽  
Author(s):  
Sandro José Baptista ◽  
Magali Christe Cammarota ◽  
Denize Dias de Carvalho Freire
Keyword(s):  
Crude Oil ◽  
Petroleum Hydrocarbons ◽  
Clay Soil ◽  
Fixed Bed ◽  
Bacterial Count ◽  
Fixed Bed Reactor ◽  
Total Petroleum Hydrocarbons ◽  
Co2 Production ◽  
Oil And Grease ◽  
Before And After

The aim of the present work was to evaluate the biodegradation of petroleum hydrocarbons in clay soil a 45-days experiment. The experiment was conducted using an aerobic fixed bed reactor, containing 300g of contaminated soil at room temperature with an air rate of 6 L/h. The growth medium was supplemented with 2.5% (w/w) (NH4)2SO4 and 0.035% (w/w) KH2PO4. Biodegradation of the crude oil in the contaminated clay soil was monitored by measuring CO2 production and removal of organic matter (OM), oil and grease (OandG), and total petroleum hydrocarbons (TPH), measured before and after the 45-days experiment, together with total heterotrophic and hydrocarbon-degrading bacterial count. The best removals of OM (50%), OandG (37%) and TPH (45%) were obtained in the bioreactors in which the highest CO2 production was achieved.

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