scholarly journals Field Test of In Situ Groundwater Treatment Applying Oxygen Diffusion and Bioaugmentation Methods in an Area with Sustained Total Petroleum Hydrocarbon (TPH) Contaminant Flow

Water ◽  
2022 ◽  
Vol 14 (2) ◽  
pp. 192
Author(s):  
Artem Demenev ◽  
Nikolay Maksimovich ◽  
Vadim Khmurchik ◽  
Gennadiy Rogovskiy ◽  
Anatoliy Rogovskiy ◽  
...  

Contamination of groundwater by petroleum hydrocarbons is a widespread environmental problem in many regions. Contamination of unsaturated and saturated zones could also pose a significant risk to human health. The main purpose of the study was to assess the efficiency of biodegradation of total petroleum hydrocarbon (TPH) in situ, in an area with loam and sandy loam soils, and to identify features and characteristics related to groundwater treatment in an area with a persistent flow of pollutants. We used methods of biostimulation (oxygen as stimulatory supplement) and bioaugmentation to improve water quality. Oxygen was added to the groundwater by diffusion through silicone tubing. The efficiency of groundwater treatment was determined by detailed monitoring. Implementation of the applied measure resulted in an average reduction in TPH concentration of 73.1% compared with the initial average concentration (4.33 mg/L), and in the local area, TPH content was reduced by 95.5%. The authors hope that this paper will contribute to a better understanding of the topic of groundwater treatment by in situ biodegradation of TPH. Further studies on this topic are particularly needed to provide more data and details on the efficiency of groundwater treatment under adverse geological conditions.

2021 ◽  
Vol 26 (51) ◽  
pp. 77-81
Author(s):  
Vesna Teofilović ◽  
Srđan Miletić ◽  
Milica Živković ◽  
Nataša Stojić ◽  
Mira Pucarević ◽  
...  

Microplastics have reached all corners of our planet, including soil and water. Plastic-degrading bacteria are seen as a promising, environmentally friendly tool for the bioremediation of soil polluted with microplastics. The petroleum origin of plastics makes them candidates for bioremediation analogous to the bioremediation of soil polluted with oil and its derivatives. A mud pit, located near the village of Turija, used for mud formation for the lubrication of drill pipes for drilling rigs, ended up polluted with oil and its derivatives. It was bioremediated using the in situ procedure. The content of n-hexane extractable substance, total petroleum hydrocarbon, dry substance, and loss on ignition were analyzed.


2021 ◽  
pp. 125995
Author(s):  
So Yeon Yoon ◽  
Seok Byum Jang ◽  
Kien Tiek Wong ◽  
Hyeseong Kim ◽  
Min Ji Kim ◽  
...  

2021 ◽  
Author(s):  
Qinggeer BORJIGIN ◽  
Bizhou ZHANG ◽  
Xiaofang Yu ◽  
Julin Gao ◽  
Xin ZHANG ◽  
...  

Abstract A lignocellulolytic microbial consortium holds promise for the in situ biodegradation of crop straw and the comprehensive and effective utilization of agricultural waste. In this study, we applied metagenomics technology to comprehensively explore the metabolic functional potential and taxonomic diversity of the microbial consortia CS (cultured on corn stover) and FP (cultured on filter paper).Analyses of the metagenomics taxonomic affiliation data showed considerable differences in the taxonomic composition and functional profile of the microbial consortia CS and FP. The microbial consortia CS primarily contained members from the genera Pseudomonas, Stenotrophomonas, Achromobacter, Dysgonomonas, Flavobacterium and Sphingobacterium, as well as Cellvibrio, Azospirillum, Pseudomonas, Dysgonomonas and Cellulomonas in FP. The COG and KEGG annotation analyses revealed considerable levels of diversity. Further analysis determined that the CS consortium had an increase in the acid and ester metabolism pathways, while carbohydrate metabolism was enriched in the FP consortium. Furthermore, a comparison against the CAZy database showed that the microbial consortia CS and FP contain a rich diversity of lignocellulose degrading families, in which GH5, GH6, GH9, GH10, GH11, GH26, GH42, and GH43 were enriched in the FP consortium, and GH44, GH28, GH2, and GH29 increased in the CS consortium. The degradative mechanism of lignocellulose metabolism by the two microbial consortia is similar, but the annotation of quantity of genes indicated that they are diverse and vary greatly. The lignocellulolytic microbial consortia cultured under different carbon conditions (CS and FP) differed substantially in their composition of the microbial community at the genus level. The changes in functional diversity were accompanied with variation in the composition of microorganisms, many of which are related to the degradation of lignocellulolytic materials. The genera Pseudomonas, Dysgonomonas and Sphingobacterium in CS and the genera Cellvibrio and Pseudomonas in FP exhibited a much wider distribution of lignocellulose degradative ability.


2018 ◽  
Vol 3 (1) ◽  
pp. 28-44
Author(s):  
Dinesh Khadka ◽  
Sushil Lamichhane ◽  
Amit P Timilsina ◽  
Bandhu R Baral ◽  
Kamal Sah ◽  
...  

Soil pit digging and their precise study is a decision making tool to assess history and future of soil management of a particular area. Thus, the present study was carried out to differentiate soil physico-chemical properties in the different layers of excavated pit of the National Maize Research Program, Rampur, Chitwan, Nepal. Eight pits were dug randomly from three blocks at a depth of 0 to 100 cm. The soil parameters were determined in-situ, and in laboratory for texture, pH, OM, N, P (as P2O5), K (as K2O), Ca, Mg, S, B, Fe, Zn, Cu and Mn of collected soils samples of different layers following standard analytical methods at Soil Science Division, Khumaltar. The result revealed that soil structure was sub-angular in majority of the layers, whereas bottom layer was single grained. The value and chrome of colour was increasing in order from surface to bottom in the majority pits. Similarly, the texture was sandy loam in majority layers of the pits. Moreover, four types of consistence (loose to firm) were observed. Furthermore, mottles and gravels were absent in the majority layers. Likewise, soil was very to moderately acidic in observed layers of majority pits, except bottom layer of agronomy block was slightly acidic. Regarding fertility parameters (OM, macro and micronutrients), some were increasing and vice-versa, while others were intermittent also. Therefore, a single layer is not dominant for particular soil physico-chemical parameters in the farm. In overall, surface layer is more fertile than rest of the layers in all the pits.     


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