Faculty Opinions recommendation of Down-regulation of the homeodomain factor Cdx2 in colorectal cancer by collagen type I: an active role for the tumor environment in malignant tumor progression.
We have found an association of miR-122 down-regulation with myocardial fibrosis in AS patients, probably through TGF-β1 up-regulation and stimulation of the enzymatic systems involved in extracellular collagen type I synthesis and deposition.
The biosynthesis of type I, type V and type VI collagens was studied by incubation of calf corneas in vitro with [3H]proline as a marker. Pepsin-solubilized collagen types were isolated by salt fractionation and quantified by SDS/PAGE. Expressed as proportions of the total hydroxyproline solubilized, corneal stroma comprised 75% type I, 8% type V and 17% type VI collagen. The rates of [3H]proline incorporation, linear up to 24 h for each collagen type, were highest for type VI collagen and lowest for type I collagen. From pulse-chase experiments, the calculated apparent half-lives for types I, V and VI collagens were 36 h, 10 h and 6 h respectively.
Down-regulation of BRCA2 expression by collagen type I promotes prostate cancer cell proliferation.