Faculty Opinions recommendation of Community epidemiology of human metapneumovirus, human coronavirus NL63, and other respiratory viruses in healthy preschool-aged children using parent-collected specimens.

ABSTRACTBroad testing for respiratory viruses among persons under investigation (PUIs) for SARS-CoV-2 has been performed inconsistently, limiting our understanding of alternative viral infections and coinfections in these patients. RNA metagenomic next-generation sequencing (mNGS) offers an agnostic tool for the detection of both SARS-CoV-2 and other RNA respiratory viruses in PUIs. Here, we used RNA mNGS to assess the frequencies of alternative viral infections in SARS-CoV-2 RT-PCR-negative PUIs (n = 30) and viral coinfections in SARS-CoV-2 RT-PCR-positive PUIs (n = 45). mNGS identified all viruses detected by routine clinical testing (influenza A [n = 3], human metapneumovirus [n = 2], and human coronavirus OC43 [n = 2], and human coronavirus HKU1 [n = 1]). mNGS also identified both coinfections (1, 2.2%) and alternative viral infections (4, 13.3%) that were not detected by routine clinical workup (respiratory syncytial virus [n = 3], human metapneumovirus [n = 1], and human coronavirus NL63 [n = 1]). Among SARS-CoV-2 RT-PCR-positive PUIs, lower cycle threshold (CT) values correlated with greater SARS-CoV-2 read recovery by mNGS (R2, 0.65; P < 0.001). Our results suggest that current broad-spectrum molecular testing algorithms identify most respiratory viral infections among SARS-CoV-2 PUIs, when available and implemented consistently.

Download Full-text

Detection and clinical manifestations of twelve respiratory viruses in hospitalized children with acute lower respiratory tract infections : Focus on human metapneumovirus, human rhinovirus and human coronavirus

Korean Journal of Pediatrics ◽

10.3345/kjp.2008.51.8.834 ◽

2008 ◽

Vol 51 (8) ◽

pp. 834 ◽

Cited By ~ 15

Author(s):

Kum Hyang Kim ◽

Jung Ho Lee ◽

Dong Shin Sun ◽

Yong Bae Kim ◽

Young Jin Choi ◽

...

Keyword(s):

Respiratory Tract ◽

Lower Respiratory Tract ◽

Respiratory Tract Infections ◽

Clinical Manifestations ◽

Respiratory Viruses ◽

Human Metapneumovirus ◽

Hospitalized Children ◽

Lower Respiratory Tract Infections ◽

Human Coronavirus ◽

Tract Infections

Download Full-text

Human Metapneumovirus: Etiological Agent of Severe Acute Respiratory Infections in Hospitalized and Deceased Patients with a Negative Diagnosis of Influenza

Pathogens ◽

10.3390/pathogens9020085 ◽

2020 ◽

Vol 9 (2) ◽

pp. 85

Author(s):

Gisela Barrera-Badillo ◽

Beatriz Olivares-Flores ◽

Adriana Ruiz-López ◽

Miguel Ángel Fierro-Valdez ◽

Rosaura Idania Gutiérrez-Vargas ◽

...

Keyword(s):

Young Children ◽

Age Groups ◽

Simultaneous Detection ◽

Respiratory Viruses ◽

Human Metapneumovirus ◽

Late Winter ◽

Older Individuals ◽

Severe Acute Respiratory Infection ◽

Syncytial Virus ◽

Tract Infections

Human metapneumovirus (HMPV) is one of the four major viral pathogens associated with acute respiratory tract infections (ARI) and creates a substantial burden of disease, particularly in young children (<5 years) and older individuals (≥65 years). The objective of this study was to determine the epidemiological behavior of HMPV in Mexico. This retrospective study was conducted over a nine-year period and used 7283 influenza-negative respiratory samples from hospitalized and deceased patients who presented Severe Acute Respiratory Infection (SARI). The samples were processed with the help of qualitative multiplex RT-PCR for simultaneous detection of 14 respiratory viruses (xTAG® RVP FAST v2). 40.8% of the samples were positive for respiratory viruses, mainly rhinovirus/enterovirus (47.6%), respiratory syncytial virus (15.9%), HMPV (11.1%) and parainfluenza virus (8.9%). Other respiratory viruses and co-infections accounted for 16.5%. HMPV infects all age groups, but the most affected group was infants between 29 days and 9 years of age (65.6%) and adults who are 40 years and older (25.7%). HMPV circulates every year from November to April, and the highest circulation was observed in late winter. The results of this study aim to raise awareness among clinicians about the high epidemiological impact of HMPV in young children and older individuals in order to reduce the economic burden in terms of health care costs.

Download Full-text

Deteksi Virus Penyebab Infeksi Saluran Pernafasan Akut di Rumah Sakit (Studi Pendahuluan dengan Uji Fast-Track® Diagnostik)

Media Penelitian dan Pengembangan Kesehatan ◽

10.22435/mpk.v28i4.257 ◽

2018 ◽

Vol 28 (4) ◽

pp. 257-262 ◽

Cited By ~ 1

Author(s):

Vivi Setiawaty ◽

Maretra Anindya Puspaningrum ◽

Arie Ardiansyah Nugraha ◽

Daniel Joko Wahyono

Keyword(s):

Laboratory Testing ◽

Fast Track ◽

Human Adenovirus ◽

Human Metapneumovirus ◽

Human Rhinovirus ◽

Parainfluenza Virus ◽

Influenza B ◽

Rt Pcr ◽

Human Coronavirus ◽

Human Parainfluenza Virus

Abstract Acute respiratory infections (ARI) is the leading cause of morbidity and mortality in the world and Indonesia. Information on the virus that causes ARI is still limited. The aim of this study was to detect the virus that causes ARI hospitalized cases in three sentinel surveillance hospitals of severe ARI. Laboratory testing of 30 nasal and throat swab specimens from ARI hospitalized cases at Deli Serdang Hospital, Wonosari Hospital and Kanudjoso Djati Hospital during August - September 2016. Laboratory testing were carried out at the Virology Laboratory of the Center for Biomedical Research and Development and Basic Health Technology. This research is a preliminary study using Fast-Track Diagnostics multiplex Real-time RT-PCR to detect 21 viruses. The viruses that have been detected are Human Metapneumovirus (21.2%), Human Parainfluenza Virus 1 (12.1%), Influenza B (6.1%), Human Coronavirus-OC43 (6.1%), Human CoronavirusNL63 (6.1%), Human Parainfluenza Virus 2 (3.0%), Human Rhinovirus (3.0%), and Human Adenovirus (3.0%). Of the 17 samples that tested positive for viruses, 14 of them were single cases of infection while the other three were cases of co-infection between Human Coronavirus-NL63 and Human Parainfluenza Virus 1, Human Metapneumovirus with Human Coronavirus-OC43, and Human Adenovirus with Human Rhinovirus. The most detected virus from ARI hospitalized cases are the Human Metapneumovirus. Abstrak Infeksi saluran pernafasan akut (ISPA) merupakan penyakit menular yang menjadi penyebab utama 1 morbiditas dan mortalitas di dunia dan Indonesia. Informasi virus penyebab ISPA masih terbatas. Tujuan dari penelitian ini adalah mendeteksi virus penyebab kasus ISPA rawat inap di tiga rumah sakit sentinel surveilans ISPA berat. Pemeriksaan pada 30 spesimen swab hidung dan tenggorok dari kasus ISPA rawat inap di RSUD Deli Serdang, RSUD Wonosari, dan RS Kanudjoso Djati selama bulan Agustus–September 2016. Pemeriksaan dilakukan di Laboratorium Virologi Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan. Penelitian ini merupakan penelitian pendahuluan menggunakan FastTrack Diagnostics multiplex Real-time RT-PCR untuk mendeteksi 21 virus. Virus-virus yang berhasil dideteksi adalah Human Metapneumovirus (21,2%). Human Parainfluenza Virus 1 (12,1%), Influenza B (6,1%), Human Coronavirus-OC43 (6,1%), Human Coronavirus-NL63 (6,1%), Human Parainfluenza Virus 2 (3,0%), Human Rhinovirus (3,0%), dan Human Adenovirus (3,0%). Dari 17 sampel yang dinyatakan positif mengandung virus, 14 diantaranya merupakan kasus infeksi tunggal sedangkan tiga lainnya merupakan kas us koinfeksi antara Human Coronavirus-NL63 dengan Human Parainfluenza Virus 1, Human Metapneumovirus dengan Human Coronavirus-OC43, dan Human Adenovirus dengan Human Rhinovirus. Virus yang paling banyak terdeteksi dari spesimen kasus ISPA rawat inap adalah Human Metapneumovirus.

Download Full-text

Evidence for respiratory viruses interactions in asymptomatic preschool-aged children

Allergologia et Immunopathologia ◽

10.1016/j.aller.2018.07.005 ◽

2019 ◽

Vol 47 (3) ◽

pp. 260-264

Author(s):

K. Douros ◽

D. Kotzia ◽

C. Kottaridi ◽

A. Giotas ◽

B. Boutopoulou ◽

...

Keyword(s):

Respiratory Viruses ◽

Preschool Aged Children

Download Full-text

Human Metapneumovirus and Human Coronavirus NL63

PEDIATRICS ◽

10.1542/peds.2007-3356 ◽

2008 ◽

Vol 121 (2) ◽

pp. 445-446 ◽

Cited By ~ 2

Author(s):

D. A. Pilger ◽

V. V. Cantarelli

Keyword(s):

Human Metapneumovirus ◽

Human Coronavirus

Download Full-text

Viruses causing lower respiratory symptoms in young children: findings from the ORChID birth cohort

Thorax ◽

10.1136/thoraxjnl-2017-210233 ◽

2017 ◽

Vol 73 (10) ◽

pp. 969-979 ◽

Cited By ~ 18

Author(s):

Mohinder Sarna ◽

Stephen B Lambert ◽

Theo P Sloots ◽

David M Whiley ◽

Asma Alsaleh ◽

...

Keyword(s):

Respiratory Syncytial Virus ◽

Young Children ◽

Birth Cohort ◽

Respiratory Symptoms ◽

Respiratory Viruses ◽

Human Metapneumovirus ◽

Birth Cohort Study ◽

Healthy Children ◽

Nasal Swabs ◽

Syncytial Virus

IntroductionViral acute respiratory infections (ARIs) cause substantial child morbidity. Sensitive molecular-based assays aid virus detection, but the clinical significance of positive tests remains uncertain as some viruses may be found in both acutely ill and healthy children. We describe disease-pathogen associations of respiratory viruses and quantify virus-specific attributable risk of ARIs in healthy children during the first 2 years of life.MethodsOne hundred fifty-eight term newborn babies in Brisbane, Australia, were recruited progressively into a longitudinal, community-based, birth cohort study conducted between September 2010 and October 2014. A daily tick-box diary captured predefined respiratory symptoms from birth until their second birthday. Weekly parent-collected nasal swabs were batch-tested for 17 respiratory viruses by PCR assays, allowing calculation of virus-specific attributable fractions in the exposed (AFE) to determine the proportion of virus-positive children whose ARI symptoms could be attributed to that particular virus.ResultsOf 8100 nasal swabs analysed, 2646 (32.7%) were virus-positive (275 virus codetections, 3.4%), with human rhinoviruses accounting for 2058/2646 (77.8%) positive swabs. Viruses were detected in 1154/1530 (75.4%) ARI episodes and in 984/4308 (22.8%) swabs from asymptomatic periods. Respiratory syncytial virus (AFE: 68% (95% CI 45% to 82%)) and human metapneumovirus (AFE: 69% (95% CI 43% to 83%)) were strongly associated with higher risk of lower respiratory symptoms.DiscussionThe strong association of respiratory syncytial virus and human metapneumovirus with ARIs and lower respiratory symptoms in young children managed within the community indicates successful development of vaccines against these two viruses should provide substantial health benefits.

Download Full-text

Investigation of the role of major respiratory viruses in the aetiology of nasal polyps using polymerase chain reaction technique

The Journal of Laryngology & Otology ◽

10.1017/s0022215114000681 ◽

2014 ◽

Vol 128 (4) ◽

pp. 356-359

Author(s):

F Aksoy ◽

A Yenigun ◽

R Dogan ◽

F Yilmaz ◽

O Ozturan ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Nasal Polyps ◽

Nasal Polyposis ◽

Respiratory Viruses ◽

Polymerase Chain Reaction Technique ◽

Control Group ◽

Chain Reaction ◽

Human Coronavirus ◽

Polymerase Chain

AbstractObjective:We aimed to identify the role of major respiratory viruses in the aetiology of human nasal polyps using polymerase chain reaction technique.Methods:Thirty patients with nasal polyps and a group of 20 healthy patients (control group) were included in this study. Mucosa was obtained from the polyps of patients with nasal polyposis and from the middle turbinate of the control group patients by means of biopsy. The samples were stored at −80 °C until molecular analysis by polymerase chain reaction was carried out.Results:In the control group, the human coronavirus and human rhinovirus were diagnosed in one of the patients and the human respiratory syncytial virus in another. In the group with nasal polyposis, the influenza B virus was identified in one of the patients and the human coronavirus in another.Conclusion:The results did not demonstrate a statistically significant relationship between nasal polyposis and respiratory viruses.

Download Full-text