Faculty Opinions recommendation of Quantitative metabolomic profiling of serum, plasma, and urine by (1)H NMR spectroscopy discriminates between patients with inflammatory bowel disease and healthy individuals.

ObjectiveAn inadequate host response to the intestinal microbiota likely contributes to the manifestation and progression of human inflammatory bowel disease (IBD). However, molecular approaches to unravelling the nature of the defective crosstalk and its consequences for intestinal metabolic and immunological networks are lacking. We assessed the mucosal transcript levels, splicing architecture and mucosa-attached microbial communities of patients with IBD to obtain a comprehensive view of the underlying, hitherto poorly characterised interactions, and how these are altered in IBD.DesignMucosal biopsies from Crohn's disease and patients with UC, disease controls and healthy individuals (n=63) were subjected to microbiome, transcriptome and splicing analysis, employing next-generation sequencing. The three data levels were integrated by different bioinformatic approaches, including systems biology-inspired network and pathway analysis.ResultsMicrobiota, host transcript levels and host splicing patterns were influenced most strongly by tissue differences, followed by the effect of inflammation. Both factors point towards a substantial disease-related alteration of metabolic processes. We also observed a strong enrichment of splicing events in inflamed tissues, accompanied by an alteration of the mucosa-attached bacterial taxa. Finally, we noted a striking uncoupling of the three molecular entities when moving from healthy individuals via disease controls to patients with IBD.ConclusionsOur results provide strong evidence that the interplay between microbiome and host transcriptome, which normally characterises a state of intestinal homeostasis, is drastically perturbed in Crohn's disease and UC. Consequently, integrating multiple OMICs levels appears to be a promising approach to further disentangle the complexity of IBD.

Download Full-text

Su1804 Alterations in Lipid, Carbohydrate, and Energy Metabolism Distinguish Inflammatory Bowel Disease Patients From Healthy Controls by Metabolomic Profiling

Gastroenterology ◽

10.1016/s0016-5085(16)31903-5 ◽

2016 ◽

Vol 150 (4) ◽

pp. S557

Author(s):

Elizabeth A. Scoville ◽

Caroline T. Brown ◽

Margaret M. Allaman ◽

Amy Motley ◽

Sara N. Horst ◽

...

Keyword(s):

Inflammatory Bowel Disease ◽

Energy Metabolism ◽

Bowel Disease ◽

Healthy Controls ◽

Metabolomic Profiling ◽

Inflammatory Bowel

Download Full-text

Thromboelastography in Patients with Inflammatory Bowel Disease

Gastroenterology Research and Practice ◽

10.1155/2020/3245657 ◽

2020 ◽

Vol 2020 ◽

pp. 1-5

Author(s):

Yonghua Shen ◽

Liangliang Shi ◽

Juanjuan Zhang ◽

Hao Zhu ◽

Yuling Yao ◽

...

Keyword(s):

Inflammatory Bowel Disease ◽

Bowel Disease ◽

Active Phase ◽

Chinese Patients ◽

Blood Levels ◽

Healthy Individuals ◽

Mean Values ◽

Venous Thromboembolic Events ◽

Venous Thromboembolic ◽

Inflammatory Bowel

Purpose. Patients with inflammatory bowel disease (IBD) frequently suffer from venous thromboembolic events, and the risk of thromboembolism increases along with disease activity. This study was conducted to discover novel thrombophilic markers using thromboelastography (TEG) and to evaluate the relation between the predisposing factors and the activity of disease in Chinese patients with Crohn’s disease (CD) and ulcerative colitis (UC). Methods. Thirty-four patients with CD, 29 patients with UC, and 53 healthy volunteers were enrolled into this study. Blood levels of R, K, α Angle, G, maximal amplitude (MA), and LY30 with TEG were determined. Results. Mean values of R, K, α Angle, G, and MA were significantly different in patients with CD and UC compared with the healthy individuals. Patients with active CD had different K, α Angle, G, and MA levels compared with patients in remission (P<0.05, P<0.001, P<0.001, and P<0.001). Levels of R, α Angle, G, and MA were also significantly different in active UC patients compared with those in remission (P<0.01, P<0.001, P<0.001, and P<0.001). Except for the G level in the CD group, differences in all TEG levels between healthy individuals and IBD patients in remission were not statistically significant. No statistical differences were observed in LY30 among patients with active phase, patients in remission, and the healthy individuals. Conclusion. Thrombophilic defects are common in Chinese patients with IBD, and TEG can be considered a new direction to anticoagulant thromboprophylaxis in IBD.

Download Full-text

On the Prevalence of M. avium Subspecies paratuberculosis DNA in the Blood of Healthy Individuals and Patients with Inflammatory Bowel Disease

PLoS ONE ◽

10.1371/journal.pone.0002537 ◽

2008 ◽

Vol 3 (7) ◽

pp. e2537 ◽

Cited By ~ 39

Author(s):

Ramon A. Juste ◽

Natalia Elguezabal ◽

Joseba M. Garrido ◽

Andres Pavon ◽

Maria V. Geijo ◽

...

Keyword(s):

Inflammatory Bowel Disease ◽

Bowel Disease ◽

Healthy Individuals ◽

Inflammatory Bowel

Download Full-text

Plasma asprosin, CCDC80 and ANGPTL4 levels are associated with metabolic and cardiovascular risk in patients with inflammatory bowel disease

Physiological Research ◽

10.33549/physiolres.934547 ◽

2021 ◽

pp. 203-211

Author(s):

Hao-Hua Wang ◽

Wan-Ying Luo ◽

Min Lin ◽

Xiao-Jing Li ◽

Guang-Da Xiang ◽

...

Keyword(s):

Insulin Resistance ◽

Inflammatory Bowel Disease ◽

Bowel Disease ◽

Early Stage ◽

Reactive Hyperemia ◽

Enzyme Linked Immunosorbent Assay ◽

Model Assessment ◽

Healthy Individuals ◽

Brachial Artery Diameter ◽

Inflammatory Bowel

Asprosin, coiled-coil domain-containing 80(CCDC80) and angiopoietin-like 4(ANGPTL4) are newly discovered adipocytokine that affects glucose tolerance, insulin resistance and cardiovascular diseases. The goal of this study was to investigate if a relationship exists among asprosin, CCDC80 and ANGPTL4 and inflammatory bowel disease (IBD). Fifty subjects with newly diagnosed IBD and fifty healthy individuals were enrolled. Patients were treated with standard therapies for 3 months. Plasma asprosin, CCDC80 and ANGPTL4 levels were measured with enzyme-linked immunosorbent assay. High resolution ultrasound was used to measure brachial artery diameter at rest, after reactive hyperemia (flow-mediated dilation, FMD) and after sublingual glyceryltrinitrate. Compare with healthy individuals, plasma CCDC80, erythrocyte sedi¬mentation rate (ESR), C-reactive protein (CRP) levels and homeostasis model assessment of insulin resistance (HOMA-IR) were significantly higher (p < 0.05, respectively), whereas plasma asprosin, ANGPTL4 levels and FMD were significantly lower in both UC and CD patients (p < 0.05). Plasma CCDC80 levels were significantly higher in patients with CD (p < 0.05), while plasma asprosin and ANGPTL4 levels were lower (pP < 0.05) as compared with those in patients with UC. Standard therapies increased plasma asprosin, ANGPTL4 levels and FMD in both UC and CD (p < 0.05), UC and CD patientswhile decreased plasma CCDC80, ESR, CRP levels and HOMA-IR (p < 0.05). The changes in HOMA-IR and FMD were correlated with the changes in plasma asprosin, CCDC80 and ANGPTL4 levels over the study period (p < 0.05). Plasma asprosin, CCDC80 and ANGPTL4 levels may be applied as a significant marker for early stage of insulin resistance and atherosclerosis in IBD, especially of CD.

Download Full-text

PTU-093 Metabolomic profiling in inflammatory bowel disease

Gut ◽

10.1136/gutjnl-2015-309861.208 ◽

2015 ◽

Vol 64 (Suppl 1) ◽

pp. A102.1-A102

Author(s):

DR Hildebrand ◽

D Trivedi ◽

Y Xu ◽

N Rattray ◽

NP Ross ◽

...

Keyword(s):

Inflammatory Bowel Disease ◽

Bowel Disease ◽

Metabolomic Profiling ◽

Inflammatory Bowel

Download Full-text

Asynchronous Replication in Lymphocytes from Patients with Inflammatory Bowel Disease and Primary Sclerosing Cholangitis

Cytogenetic and Genome Research ◽

10.1159/000381406 ◽

2015 ◽

Vol 145 (1) ◽

pp. 35-41 ◽

Cited By ~ 1

Author(s):

Ido Laish ◽

Tal Biron-Shental ◽

Hila Katz ◽

Meytal Liberman ◽

Yona Kitay-Cohen ◽

...

Keyword(s):

Inflammatory Bowel Disease ◽

Primary Sclerosing Cholangitis ◽

Bowel Disease ◽

Sclerosing Cholangitis ◽

Replication Timing ◽

Peripheral Blood Lymphocytes ◽

Malignant Potential ◽

Healthy Individuals ◽

Temporal Differences ◽

Inflammatory Bowel

Primary sclerosing cholangitis (PSC) and inflammatory bowel disease (IBD) are associated chronic inflammatory diseases with malignant potential. Loss of replication synchrony during the S-phase of the cell cycle has been shown to be linked to several malignant and premalignant states. This study evaluated temporal differences in replication timing between these diseases. The replication pattern of peripheral blood lymphocytes obtained from patients with PSC and IBD and healthy individuals was analyzed by fluorescence in situ hybridization (FISH) in 2 pairs of alleles, in 15qter and 13qter. Asynchrony was determined by the presence of 1 single and 1 set of double dots in the same cell. Samples from subjects with PSC showed significantly greater temporal differences in replication timing, in contrast to the high level of synchrony observed in samples from healthy individuals (p = 0.045). Samples from IBD patients exhibited a nonsignificant increase in replication asynchrony. We believe that these results reflect impairment in the replication control of structural homologous loci in PSC, and that this phenomenon may be correlated with the inflammation-induced malignant potential of this condition.

Download Full-text

Microbial imbalance in inflammatory bowel disease patients at different taxonomic levels

Gut Pathogens ◽

10.1186/s13099-019-0341-6 ◽

2020 ◽

Vol 12 (1) ◽

Cited By ~ 20

Author(s):

Mohammad Tauqeer Alam ◽

Gregory C. A. Amos ◽

Andrew R. J. Murphy ◽

Simon Murch ◽

Elizabeth M. H. Wellington ◽

...

Keyword(s):

Inflammatory Bowel Disease ◽

Gut Microbiota ◽

Bowel Disease ◽

Gut Microbiome ◽

Global Scale ◽

Rrna Gene ◽

Healthy Individuals ◽

Bacterial Phyla ◽

Inflammatory Bowel ◽

Bacterial Groups

Abstract Background Inflammatory bowel disease (IBD), is a debilitating group of chronic diseases including Crohn’s Disease (CD) and ulcerative colitis (UC), which causes inflammation of the gut and affects millions of people worldwide. At different taxonomic levels, the structure of the gut microbiota is significantly altered in IBD patients compared to that of healthy individuals. However, it is unclear how these IBD-affected bacterial groups are related to other common bacteria in the gut, and how they are connected across different disease conditions at the global scale. Results In this study, using faecal samples from patients with IBD, we show through diversity analysis of the microbial community structure based on the 16S rRNA gene that the gut microbiome of IBD patients is less diverse compared to healthy individuals. Furthermore, we have identified which bacterial groups change in abundance in both CD and UC compared to healthy controls. A substantial imbalance was observed across four major bacterial phyla including Firmicutes, Bacteroidetes, Proteobacteria and Actinobacteria, which together constitute > 98% of the gut microbiota. Next, we reconstructed a bacterial family co-abundance network based on the correlation of abundance profiles obtained from the public gut microbiome data of > 22,000 samples of faecal and gut biopsies taken from both diseased and healthy individuals. The data was compiled using the EBI metagenomics database (Mitchell et al. in Nucleic Acids Res 46:D726–D735, 2018). By mapping IBD-altered bacterial families to the network, we show that the bacterial families which exhibit an increased abundance in IBD conditions are not well connected to other groups, implying that these families generally do not coexist together with common gut organisms. Whereas, the bacterial families whose abundance is reduced or did not change in IBD conditions compared to healthy conditions are very well connected to other bacterial groups, suggesting they are highly important groups of bacteria in the gut that can coexist with other bacteria across a range of conditions. Conclusions IBD patients exhibited a less diverse gut microbiome compared to healthy individuals. Bacterial groups which changed in IBD patients were found to be groups which do not co-exist well with common commensal gut bacteria, whereas bacterial groups which did not change in patients with IBD were found to commonly co-exist with commensal gut microbiota. This gives a potential insight into the dynamics of the gut microbiota in patients with IBD.

Download Full-text