Faculty Opinions recommendation of Highly photostable, reversibly photoswitchable fluorescent protein with high contrast ratio for live-cell superresolution microscopy.

2016 ◽  
Author(s):  
Martin Gruebele ◽  
Max Platkov
Keyword(s):  
Fluorescent Protein ◽  
Contrast Ratio ◽  
Live Cell ◽  
High Contrast ◽  
Superresolution Microscopy

scholarly journals Highly photostable, reversibly photoswitchable fluorescent protein with high contrast ratio for live-cell superresolution microscopy

2016 ◽  
Vol 113 (37) ◽  
pp. 10364-10369 ◽  
Author(s):  
Xi Zhang ◽  
Mingshu Zhang ◽  
Dong Li ◽  
Wenting He ◽  
Jianxin Peng ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Protein ◽  
Contrast Ratio ◽  
Live Cell ◽  
Acquisition Time ◽  
Fluorescence Signal ◽  
Effective Quantum Yield ◽  
Structured Illumination ◽  
Structured Illumination Microscopy

Two long-standing problems for superresolution (SR) fluorescence microscopy are high illumination intensity and long acquisition time, which significantly hamper its application for live-cell imaging. Reversibly photoswitchable fluorescent proteins (RSFPs) have made it possible to dramatically lower the illumination intensities in saturated depletion-based SR techniques, such as saturated depletion nonlinear structured illumination microscopy (NL-SIM) and reversible saturable optical fluorescence transition microscopy. The characteristics of RSFPs most critical for SR live-cell imaging include, first, the integrated fluorescence signal across each switching cycle, which depends upon the absorption cross-section, effective quantum yield, and characteristic switching time from the fluorescent “on” to “off” state; second, the fluorescence contrast ratio of on/off states; and third, the photostability under excitation and depletion. Up to now, the RSFPs of the Dronpa and rsEGFP (reversibly switchable EGFP) families have been exploited for SR imaging. However, their limited number of switching cycles, relatively low fluorescence signal, and poor contrast ratio under physiological conditions ultimately restrict their utility in time-lapse live-cell imaging and their ability to reach the desired resolution at a reasonable signal-to-noise ratio. Here, we present a truly monomeric RSFP, Skylan-NS, whose properties are optimized for the recently developed patterned activation NL-SIM, which enables low-intensity (∼100 W/cm2) live-cell SR imaging at ∼60-nm resolution at subsecond acquisition times for tens of time points over broad field of view.

scholarly journals Patterned waveguide liquid crystal displays

RSC Advances ◽  
2020 ◽  
Vol 10 (68) ◽  
pp. 41693-41702
Author(s):  
Yunho Shin ◽  
Jinghua Jiang ◽  
Guangkui Qin ◽  
Qian Wang ◽  
Ziyuan Zhou ◽  
...  
Keyword(s):  
Liquid Crystal ◽  
Switching Time ◽  
Contrast Ratio ◽  
Liquid Crystal Displays ◽  
High Contrast ◽  
High Brightness ◽  
Photo Polymerization ◽  
Ultrafast Switching ◽  
Light Waveguide ◽  
Polymer Stabilized

A polymer stabilized LC based light waveguide display is reported. Performance is improved by patterned photo-polymerization or electrode. It has high brightness, ultrafast switching time, high contrast ratio, and high transmittance for transparent and augmented displays.

scholarly journals Full-frame and high-contrast smart windows from halide-exchanged perovskites

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
You Liu ◽  
Jungan Wang ◽  
Fangfang Wang ◽  
Zhengchun Cheng ◽  
Yinyu Fang ◽  
...  
Keyword(s):  
Contrast Ratio ◽  
High Contrast ◽  
Smart Window ◽  
Frame Size ◽  
Smart Windows ◽  
Ideal Solutions ◽  
Ion Gel ◽  
And Control ◽  
Colour Rendering ◽  
Perovskite Photovoltaic

AbstractWindow glazing plays an essential role to modulate indoor light and heat transmission, which is a prospect to save the energy cost in buildings. The latest photovoltachromic technology has been regarded as one of the most ideal solutions, however, to achieve full-frame size (100% active area) and high-contrast ratio (>30% variable in visible wavelength) for smart window applicability is still a challenge. Here we report a photovoltachromic device combining full-transparent perovskite photovoltaic and ion-gel based electrochromic components in a vertical tandem architecture without any intermediated electrode. Most importantly, by accurately adjusting the halide-exchanging period, this photovoltachromic module can realize a high pristine transmittance up to 76%. Moreover, it possesses excellent colour-rendering index to 96, wide contrast ratio (>30%) on average visible transmittance (400-780 nm), and a self-adaptable transmittance adjustment and control indoor brightness and temperature automatically depending on different solar irradiances.

Stacked metal cathode for high-contrast-ratio polymeric light-emitting devices

2005 ◽  
Vol 86 (14) ◽  
pp. 143514 ◽  
Author(s):  
S. H. Li ◽  
H. Liem ◽  
C. W. Chen ◽  
E. H. Wu ◽  
Z. Xu ◽  
...  
Keyword(s):  
Contrast Ratio ◽  
High Contrast ◽  
Light Emitting ◽  
Light Emitting Devices ◽  
Metal Cathode

scholarly journals Direct visualization of Bcl-2 family protein interactions using live cell fluorescent protein redistribution assays

2012 ◽  
Vol 3 (3) ◽  
pp. e288-e288 ◽  
Author(s):  
C Wong ◽  
D J Anderson ◽  
E F Lee ◽  
W D Fairlie ◽  
M J C Ludlam
Keyword(s):  
Protein Interactions ◽  
Fluorescent Protein ◽  
Live Cell ◽  
Direct Visualization ◽  
Family Protein

scholarly journals Correlative live-cell and superresolution microscopy reveals cargo transport dynamics at microtubule intersections

2013 ◽  
Vol 110 (9) ◽  
pp. 3375-3380 ◽  
Author(s):  
Štefan Bálint ◽  
Ione Verdeny Vilanova ◽  
Ángel Sandoval Álvarez ◽  
Melike Lakadamyali
Keyword(s):  
Live Cell ◽  
Superresolution Microscopy ◽  
Transport Dynamics ◽  
Cargo Transport

scholarly journals Red-Shifted Aminated Derivatives of GFP Chromophore for Live-Cell Protein Labeling with Lipocalins

2018 ◽  
Vol 19 (12) ◽  
pp. 3778 ◽  
Author(s):  
Nina Bozhanova ◽  
Mikhail Baranov ◽  
Nadezhda Baleeva ◽  
Alexey Gavrikov ◽  
Alexander Mishin
Keyword(s):  
Green Fluorescent Protein ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Protein ◽  
Live Cell ◽  
Protein Labeling ◽  
Cell Protein ◽  
Gfp Chromophore ◽  
Green Fluorescent ◽  
Derivatives Of

Fluorogens are an attractive type of dye for imaging applications, eliminating time-consuming washout steps from staining protocols. With just a handful of reported fluorogen-protein pairs, mostly in the green region of spectra, there is a need for the expansion of their spectral range. Still, the origins of solvatochromic and fluorogenic properties of the chromophores suitable for live-cell imaging are poorly understood. Here we report on the synthesis and labeling applications of novel red-shifted fluorogenic cell-permeable green fluorescent protein (GFP) chromophore analogs.

Extreme Adaptive Optics

2018 ◽  
Vol 56 (1) ◽  
pp. 315-355 ◽  
Author(s):  
Olivier Guyon
Keyword(s):  
Adaptive Optics ◽  
Thermal Emission ◽  
Contrast Ratio ◽  
Angular Separation ◽  
Circumstellar Disks ◽  
Spectroscopic Characterization ◽  
Central Star ◽  
High Contrast ◽  
Direct Imaging ◽  
Segmented Mirror

Over the last two decades, several thousand exoplanets have been identified, and their study has become a high scientific priority. Direct imaging of nearby exoplanets and the circumstellar disks in which they form and evolve is challenging due to the high contrast ratio and small angular separation relative to the central star. Exoplanets are typically within 1 arcsec of, and between 4 and 10 orders of magnitude fainter than, the stars they orbit. To meet these challenges, ground-based telescopes must be equipped with extreme adaptive optics (ExAO) systems optimized to acquire high-contrast images of the immediate surrounding of nearby bright stars. Current ExAO systems have the sensitivity to image thermal emission from young massive planets in near-IR, while future systems deployed on Giant Segmented Mirror Telescopes will image starlight reflected by lower-mass rocky planets. Thanks to rapid progress in optical coronagraphy, wavefront control, and data analysis techniques, direct imaging and spectroscopic characterization of habitable exoplanets will be within reach of the next generation of large ground-based telescopes.

scholarly journals High-fidelity photometry and astrometry of high-contrast imaged companions using LOCI processing.

2013 ◽  
Vol 8 (S299) ◽  
pp. 44-45
Author(s):  
Jérôme Maire ◽  
Jonathan Gagné ◽  
David Lafrenière ◽  
James R. Graham ◽  
René Doyon
Keyword(s):  
Contrast Ratio ◽  
Angular Separation ◽  
Speckle Noise ◽  
High Contrast ◽  
Contrast Imaging ◽  
High Contrast Imaging ◽  
Suppression Technique ◽  
Imaging And Spectroscopy ◽  
And Migration ◽  
Imaging Performance

RésuméDirect imaging and spectroscopy of exoplanets is a key element for understanding planet formation and migration. Such direct detections and characterizations remains technologically challenging, since a very high contrast ratio and small angular separation are involved, and futhermore speckle noise limits the high-contrast imaging performance. We further discuss a speckle subtraction and suppression technique that fully takes advantage of spectral and time-domain information on quasi-static speckles to measure the highest-fidelity photometry as well as accurate astrometry of detected companions.

scholarly journals tdLanYFP, a yellow, bright, photostable and pH insensitive fluorescent protein for live cell imaging and FRET-based sensing strategies

2021 ◽  
Author(s):  
Y. Bousmah ◽  
H. Valenta ◽  
G. Bertolin ◽  
U. Singh ◽  
V. Nicolas ◽  
...  
Keyword(s):  
Single Molecule ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Yellow Fluorescent Protein ◽  
Resonance Energy ◽  
Live Cell ◽  
Live Cells

AbstractYellow fluorescent proteins (YFP) are widely used as optical reporters in Förster Resonance Energy Transfer (FRET) based biosensors. Although great improvements have been done, the sensitivity of the biosensors is still limited by the low photostability and the poor fluorescence performances of YFPs at acidic pHs. In fact, today, there is no yellow variant derived from the EYFP with a pK1/2 below ∼5.5. Here, we characterize a new yellow fluorescent protein, tdLanYFP, derived from the tetrameric protein from the cephalochordate B. lanceolatum, LanYFP. With a quantum yield of 0.92 and an extinction coefficient of 133 000 mol−1.L.cm−1, it is, to our knowledge, the brightest dimeric fluorescent protein available, and brighter than most of the monomeric YFPs. Contrasting with EYFP and its derivatives, tdLanYFP has a very high photostability in vitro and preserves this property in live cells. As a consequence, tdLanYFP allows the imaging of cellular structures with sub-diffraction resolution with STED nanoscopy. We also demonstrate that the combination of high brightness and strong photostability is compatible with the use of spectro-microscopies in single molecule regimes. Its very low pK1/2 of 3.9 makes tdLanYFP an excellent tag even at acidic pHs. Finally, we show that tdLanYFP can be a FRET partner either as donor or acceptor in different biosensing modalities. Altogether, these assets make tdLanYFPa very attractive yellow fluorescent protein for long-term or single-molecule live-cell imaging that is also suitable for FRET experiment including at acidic pH.

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