Faculty Opinions recommendation of Cell-death-independent antiviral response mediated by N resistance factor in Nicotiana benthamiana involves inhibited localization of tobamovirus movement protein to plasmodesmata.

Our previous research found that NSvc4, the movement protein of rice stripe virus (RSV), could localize to the actin filaments, endoplasmic reticulum, plasmodesmata, and chloroplast, but the roles of NSvc4 played in the chloroplast were opaque. Here, we confirm the accumulation of NSvc4 in the chloroplasts and the N-terminal 1–73 amino acids of NSvc4 are sufficient to localize to chloroplasts. We provide evidence to show that chloroplast-localized NSvc4 can impair the chloroplast-mediated immunity. Expressing NSvc4 in Nicotiana benthamiana leaves results in the decreased expression of defense-related genes NbPR1, NbPR2, and NbWRKY12 and the inhibition of chloroplast-derived ROS production. In addition, generation of an infectious clone of potato virus X (PVX) carrying NSvc4 facilitates PVX infection in N. benthamiana plants. Moreover, we identify two chloroplast-related host factors, named NbGAPDH-A and NbPsbQ1, both of which can interact with NSvc4. Knockdown of NbGAPDH-A or NbPsbQ1 can both promote RSV infection. Our results decipher a detailed function of NSvc4 in the chloroplast.

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Correction: The B cell death function of obinutuzumab-HDEL produced in plant (Nicotiana benthamiana L.) is equivalent to obinutuzumab produced in CHO cells

PLoS ONE ◽

10.1371/journal.pone.0195917 ◽

2018 ◽

Vol 13 (4) ◽

pp. e0195917

Author(s):

Jin Won Lee ◽

Woon Heo ◽

Jinu Lee ◽

Narae Jin ◽

Sei Mee Yoon ◽

...

Keyword(s):

Cell Death ◽

B Cell ◽

Cho Cells ◽

Nicotiana Benthamiana

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Cell Death of Nicotiana benthamiana Is Induced by Secreted Protein NIS1 of Colletotrichum orbiculare and Is Suppressed by a Homologue of CgDN3

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-12-11-0316 ◽

2012 ◽

Vol 25 (5) ◽

pp. 625-636 ◽

Cited By ~ 33

Author(s):

Kae Yoshino ◽

Hiroki Irieda ◽

Fumie Sugimoto ◽

Hirofumi Yoshioka ◽

Tetsuro Okuno ◽

...

Keyword(s):

Cell Death ◽

Nicotiana Benthamiana ◽

Protein Gene ◽

Fluorescent Protein ◽

Expression System ◽

Functional Screening ◽

Secreted Protein ◽

Virus Induced Gene Silencing ◽

Colletotrichum Orbiculare ◽

Green Fluorescent

Colletotrichum orbiculare, the causal agent of cucumber anthracnose, infects Nicotiana benthamiana. Functional screening of C. orbiculare cDNAs in a virus vector-based plant expression system identified a novel secreted protein gene, NIS1, whose product induces cell death in N. benthamiana. Putative homologues of NIS1 are present in selected members of fungi belonging to class Sordariomycetes, Dothideomycetes, or Orbiliomycetes. Green fluorescent protein–based expression studies suggested that NIS1 is preferentially expressed in biotrophic invasive hyphae. NIS1 lacking signal peptide did not induce NIS1-triggered cell death (NCD), suggesting apoplastic recognition of NIS1. NCD was prevented by virus-induced gene silencing of SGT1 and HSP90, indicating the dependency of NCD on SGT1 and HSP90. Deletion of NIS1 had little effect on the virulence of C. orbiculare against N. benthamiana, suggesting possible suppression of NCD by C. orbiculare at the postinvasive stage. The CgDN3 gene of C. gloeosporioides was previously identified as a secreted protein gene involved in suppression of hypersensitive-like response in Stylosanthes guianensis. Notably, we found that NCD was suppressed by the expression of a CgDN3 homologue of C. orbiculare. Our findings indicate that C. orbiculare expresses NIS1 at the postinvasive stage and suggest that NCD could be repressed via other effectors, including the CgDN3 homologue.

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Aconitase plays a role in regulating resistance to oxidative stress and cell death in Arabidopsis and Nicotiana benthamiana

Plant Molecular Biology ◽

10.1007/s11103-006-9087-x ◽

2006 ◽

Vol 63 (2) ◽

pp. 273-287 ◽

Cited By ~ 90

Author(s):

Wolfgang Moeder ◽

Olga del Pozo ◽

Duroy A. Navarre ◽

Gregory B. Martin ◽

Daniel F. Klessig

Keyword(s):

Oxidative Stress ◽

Cell Death ◽

Nicotiana Benthamiana

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Inhibition of long-distance movement of RNA silencing signals in Nicotiana benthamiana by Apple chlorotic leaf spot virus 50 kDa movement protein

Virology ◽

10.1016/j.virol.2008.09.024 ◽

2008 ◽

Vol 382 (2) ◽

pp. 199-206 ◽

Cited By ~ 15

Author(s):

Hajime Yaegashi ◽

Akihiro Tamura ◽

Masamichi Isogai ◽

Nobuyuki Yoshikawa

Keyword(s):

Leaf Spot ◽

Nicotiana Benthamiana ◽

Rna Silencing ◽

Movement Protein ◽

Long Distance ◽

Spot Virus ◽

Chlorotic Leaf ◽

Long Distance Movement

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Tobacco rattle virus 29K Movement Protein Is the Elicitor of Extreme and Hypersensitive-like Resistance in Two Cultivars of Solanum tuberosum

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-20-11-1396 ◽

2007 ◽

Vol 20 (11) ◽

pp. 1396-1405 ◽

Cited By ~ 19

Author(s):

Walid Ghazala ◽

Mark Varrelmann

Keyword(s):

Cell Death ◽

Solanum Tuberosum ◽

Virus Replication ◽

Movement Protein ◽

Tobacco Rattle Virus ◽

Cell Movement ◽

Systemic Infection ◽

Potato Virus X ◽

Host Responses ◽

Cell Death Response

Leaf infection experiments were used to analyze the host responses of Solanum tuberosum cultivars known to be resistant or susceptible to natural, nematode-mediated infection of tubers and necrosis induction (“spraing”) by Tobacco rattle virus (TRV) isolate PpK20 (TRV-PpK20). Extreme and hypersensitive-like resistance (ER and HR-like, respectively) as well as spreading veinal necrosis and systemic infection were observed. Agroinfection of leaves with a DsRed-expressing TRV cDNA clone revealed ER to function on the single-cell level, inhibiting virus replication and possessing the potential to initiate a cell death response. HR-like necrosis was characterized by initial virus replication and cell-to-cell movement before the onset of necrosis. Transient agroexpression and Potato virus X (PVX)-mediated expression assays demonstrated that the 29K-PpK20 movement protein (MP) can elicit ER and HR-like cell-death. A TRV isolate, PpO85M, known to overcome the resistance to spraing in plants that are resistant to TRV-PpK20 encoded a variant 29K protein which did not elicit HR in PpK20-HR plants. Our results show that the TRV MP is the elicitor of both ER and HR-like cell-death, that no other TRV-encoded proteins or RNA replication are required for its elicitor activity, and that the host reactions are likely to be controlled by single dominant resistance genes.

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