Faculty Opinions recommendation of The phocein homologue SmMOB3 is essential for vegetative cell fusion and sexual development in the filamentous ascomycete Sordaria macrospora.

2011 ◽  
Author(s):  
Ulrich Kueck
Keyword(s):  
Cell Fusion ◽  
Sexual Development ◽  
Vegetative Cell ◽  
Sordaria Macrospora ◽  
Filamentous Ascomycete

Cytoskeleton interactions in the ascus development and sporulation of Sordaria macrospora

1993 ◽  
Vol 104 (3) ◽  
pp. 883-898 ◽  
Author(s):  
C. Thompson-Coffe ◽  
D. Zickler
Keyword(s):  
Spindle Pole ◽  
Sordaria Macrospora ◽  
Filamentous Ascomycete ◽  
Spindle Pole Bodies ◽  
Double Label ◽  
Butanedione Monoxime ◽  
Myosin Interaction ◽  
And Migration ◽  
Relationship Of ◽  
The Relationship

The organization of actin during meiosis and sporulation in the ascus of the filamentous ascomycete Sordaria macrospora was determined by immunofluorescence without removal of the cell wall. Actin is present as a dense cortical network of microfilaments (MF) and plaques, a perinuclear shell of actin in prophase I of meiosis, and a complex array of MF involved in alignment of prespore nuclei and closure of spore cell membranes. The relationship of actin to the previously examined microtubule system of the ascus was determined by double-label immunofluorescence. The cytoskeletal inhibitors nocodazole, cytochalasin D and 2,3-butanedione monoxime were used to examine the roles of actin and myosin in ascus development. Microfilament and microtubule arrays are interdependant; disruption of one network results in abnormalities in the other. Both microfilaments and actin-myosin interaction are required for separation and migration of duplicated spindle pole bodies, septation and sporulation

Zygote giant cell differentiation in Dictyostelium discoideum: biochemical markers of specific stages of sexual development

1992 ◽  
Vol 70 (10-11) ◽  
pp. 1200-1208 ◽  
Author(s):  
Darren D. Browning ◽  
Keith E. Lewis ◽  
Danton H. O'Day
Keyword(s):  
Alkaline Phosphatase ◽  
Cell Differentiation ◽  
Giant Cell ◽  
Dictyostelium Discoideum ◽  
Cell Fusion ◽  
Sexual Development ◽  
Giant Cells ◽  
Second Phase ◽  
Developmental Phase ◽  
Calcium Dependent

Sexual development in Dictyostelium discoideum has many unique features making it an attractive eukaryotic model system for the study of biomembrane fusion and intercellular communication. The work presented here provides primary biochemical evidence for two distinct phases during early sexual development that appear to be defined by calcium-dependent gamete cell fusion. In addition, we introduce a novel procedure for the enrichment of zygote giant cells and use this method to define certain wheat-germ agglutinin binding glycoproteins which are specifically located in zygote giant cells and others which are markers for surrounding amoebae in the second phase of development. In addition, a G protein which is present in high amounts early in development is unique to giant cells in the second phase, suggesting a role in phagocytosis. Finally, alkaline phosphatase activity was found to mark the first phase of sexual development, suggesting a role in cell fusion. This contrasts with the patterns of α-mannosidase and β-glucosidase activity that increase late in the second developmental phase, where they likely function in endocyte digestion during the cytophagic period. The developmental significance of these findings is discussed.Key words: zygote giant cell differentiation, Ca2+, glycoproteins, GTP-binding proteins, alkaline phosphatase, glycosidase, cell fusion.

scholarly journals The histone chaperone ASF1 is essential for sexual development in the filamentous fungus Sordaria macrospora

2012 ◽  
Vol 84 (4) ◽  
pp. 748-765 ◽  
Author(s):  
Stefan Gesing ◽  
Daniel Schindler ◽  
Benjamin Fränzel ◽  
Dirk Wolters ◽  
Minou Nowrousian
Keyword(s):  
Filamentous Fungus ◽  
Sexual Development ◽  
Histone Chaperone ◽  
Sordaria Macrospora

scholarly journals The novel ER membrane protein PRO41 is essential for sexual development in the filamentous fungus Sordaria macrospora

2007 ◽  
Vol 64 (4) ◽  
pp. 923-937 ◽  
Author(s):  
Minou Nowrousian ◽  
Sandra Frank ◽  
Sandra Koers ◽  
Peter Strauch ◽  
Thomas Weitner ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Filamentous Fungus ◽  
Sexual Development ◽  
The Novel ◽  
Sordaria Macrospora ◽  
Er Membrane

scholarly journals Functional Characterization of MAT1-1-Specific Mating-Type Genes in the Homothallic Ascomycete Sordaria macrospora Provides New Insights into Essential and Nonessential Sexual Regulators

2010 ◽  
Vol 9 (6) ◽  
pp. 894-905 ◽  
Author(s):  
V. Klix ◽  
M. Nowrousian ◽  
C. Ringelberg ◽  
J. J. Loros ◽  
J. C. Dunlap ◽  
...  
Keyword(s):  
Mating Type ◽  
Sexual Development ◽  
Fruiting Body ◽  
Content Type ◽  
Sordaria Macrospora ◽  
Body Development ◽  
Protein Encoding ◽  
Fruiting Body Development ◽  
Mating Type Genes ◽  
Domain Protein

ABSTRACT Mating-type genes in fungi encode regulators of mating and sexual development. Heterothallic ascomycete species require different sets of mating-type genes to control nonself-recognition and mating of compatible partners of different mating types. Homothallic (self-fertile) species also carry mating-type genes in their genome that are essential for sexual development. To analyze the molecular basis of homothallism and the role of mating-type genes during fruiting-body development, we deleted each of the three genes, SmtA-1 (MAT1-1-1), SmtA-2 (MAT1-1-2), and SmtA-3 (MAT1-1-3), contained in the MAT1-1 part of the mating-type locus of the homothallic ascomycete species Sordaria macrospora. Phenotypic analysis of deletion mutants revealed that the PPF domain protein-encoding gene SmtA-2 is essential for sexual reproduction, whereas the α domain protein-encoding genes SmtA-1 and SmtA-3 play no role in fruiting-body development. By means of cross-species microarray analysis using Neurospora crassa oligonucleotide microarrays hybridized with S. macrospora targets and quantitative real-time PCR, we identified genes expressed under the control of SmtA-1 and SmtA-2. Both genes are involved in the regulation of gene expression, including that of pheromone genes.

scholarly journals Nuclear interactions in a heterokaryon: insight from the model Neurospora tetrasperma

2014 ◽  
Vol 281 (1786) ◽  
pp. 20140084 ◽  
Author(s):  
Nicklas Samils ◽  
Jonàs Oliva ◽  
Hanna Johannesson
Keyword(s):  
Sexual Development ◽  
Mycelial Growth ◽  
Developmental Stages ◽  
Tissue Type ◽  
Mating Types ◽  
Sexual Stage ◽  
Expression Ratio ◽  
Filamentous Ascomycete ◽  
Expression Bias ◽  
Neurospora Tetrasperma

A heterokaryon is a tissue type composed of cells containing genetically different nuclei. Although heterokaryosis is commonly found in nature, an understanding of the evolutionary implications of this phenomenon is largely lacking. Here, we use the filamentous ascomycete Neurospora tetrasperma to study the interplay between nuclei in heterokaryons across vegetative and sexual developmental stages. This fungus harbours nuclei of two opposite mating types ( mat A and mat a ) in the same cell and is thereby self-fertile. We used pyrosequencing of mat -linked SNPs of three heterokaryons to demonstrate that the nuclear ratio is consistently biased for mat A -nuclei during mycelial growth (mean mat A / mat a ratio 87%), but evens out during sexual development (ratio ranging from 40 to 57%) . Furthermore, we investigated the association between nuclear ratio and expression of alleles of mat -linked genes and found that expression is coregulated to obtain a tissue-specific bias in expression ratio: during mycelial extension, we found a strong bias in expression for mat A -linked genes, that was independent of nuclear ratio, whereas at the sexual stage we found an expression bias for genes of the mat a nuclei. Taken together, our data indicate that nuclei cooperate to optimize the fitness of the heterokaryon, via both altering their nuclear ratios and coregulation genes expressed in the different nuclei.

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