scholarly journals Cellular Changes in Buccal Mucosa from Farmers Exposed to Glyphosate / Alterações Celulares na Mucosa Bucal de Agricultores Expostos ao Glifosato

2021 ◽  
Vol 7 (11) ◽  
pp. 105242-105257
Author(s):  
Carine Kupske ◽  
Suzymeire Baroni ◽  
Lauren Lúcia Zamin
Keyword(s):  
Oral Mucosa ◽  
Test Group ◽  
Sociodemographic Characteristics ◽  
Family Farmers ◽  
Cellular Changes ◽  
Cellular Modifications ◽  
And Behavior ◽  
Mucosa Epithelium ◽  
Cellular Alterations ◽  
Herbicide Glyphosate

This study evaluated the sociodemographic characteristics and behavior of the oral mucosa epithelium exposured to the herbicide glyphosate of family farmers in Cerro Largo, RS, Brazil. 120 individuals were selected for social data collection through interviews. According to the results, most of the interviewees uses glyphosate between 5-10 years, being exposed between 30 minutes to one hour each application and applying the herbicide 1-2 times a year. After the interview, we selected the subjects to the  Micronucleus (MN) test. For this test, oral smears were performed in three distinct regions (cheek, mouth floor and tongue edges) of 10 test subjects (exposed to glyphosate, non-smoker and non-alcoholic) and 10 control subjects. Results showed that glyphosate exposure increased the frequency of MN in the test group (p = 0.0002), as well as the frequency of other cellular alterations, such as brokenegg (p = 0.001), binucleation (p = 0.0001) and karyolysis (p = 0.0004). Based on these findings, the extent use of glyphosate may be causing damage to the oral mucosa epithelium and this might respond adaptively through cellular modifications.

The first normal oral mucosa epithelium in which ?-glutamyl transpeptidase activity has been detected

1992 ◽  
Vol 24 (12) ◽  
pp. 964-968 ◽  
Author(s):  
A. E. Schwint ◽  
A. M. Collet ◽  
A. E. Mendez ◽  
R. L. Cabrini ◽  
M. E. Itoiz
Keyword(s):  
Oral Mucosa ◽  
Normal Oral Mucosa ◽  
Glutamyl Transpeptidase ◽  
Mucosa Epithelium

Cultivated oral mucosa epithelium transplantation (COMET) in bilateral limbal stem cell deficiency

2011 ◽  
Vol 89 (s248) ◽  
pp. 0-0 ◽  
Author(s):  
D DOBROWOLSKI ◽  
E WYLEGALA ◽  
B WOWRA ◽  
B ORZECHOWSKA‐WYLEGALA
Keyword(s):  
Stem Cell ◽  
Oral Mucosa ◽  
Limbal Stem Cell Deficiency ◽  
Limbal Stem Cell ◽  
Mucosa Epithelium

scholarly journals Type XVIII Collagen Modulates Keratohyalin Granule Formation and Keratinization in Oral Mucosa

2019 ◽  
Vol 20 (19) ◽  
pp. 4739 ◽  
Author(s):  
Ha Thi Thu Nguyen ◽  
Mitsuaki Ono ◽  
Emilio Satoshi Hara ◽  
Taishi Komori ◽  
Midori Edamatsu ◽  
...  
Keyword(s):  
Oral Mucosa ◽  
3D Culture ◽  
Squamous Carcinoma ◽  
Mrna Levels ◽  
Loss Of Function ◽  
Granule Formation ◽  
Squamous Carcinoma Cells ◽  
Cellular Modifications ◽  
Keratinized Mucosa ◽  
Epithelial Keratinization

Epithelial keratinization involves complex cellular modifications that provide protection against pathogens and chemical and mechanical injuries. In the oral cavity, keratinized mucosa is also crucial to maintain healthy periodontal or peri-implant tissues. In this study, we investigated the roles of type XVIII collagen, a collagen-glycosaminoglycan featuring an extracellular matrix component present in the basement membrane, in oral mucosal keratinization. Histological analysis of keratinized and non-keratinized oral mucosa showed that type XVIII collagen was highly expressed in keratinized mucosa. Additionally, a 3D culture system using human squamous carcinoma cells (TR146) was used to evaluate and correlate the changes in the expression of type XVIII collagen gene, COL18A1, and epithelial keratinization-related markers, e.g., keratin 1 (KRT1) and 10 (KRT10). The results showed that the increase in COL18A1 expression followed the increase in KRT1 and KRT10 mRNA levels. Additionally, loss-of-function analyses using silencing RNA targeting COL18A1 mRNA and a Col18-knockout (KO) mouse revealed that the absence of type XVIII collagen induces a dramatic decrease in KRT10 expression as well as in the number and size of keratohyalin granules. Together, the results of this study demonstrate the importance of type XVIII collagen in oral mucosal keratinization.

scholarly journals Cultivated Oral Mucosa Epithelium in Ocular Surface Reconstruction in Aniridia Patients

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Dariusz Dobrowolski ◽  
Boguslawa Orzechowska-Wylegala ◽  
Bogumil Wowra ◽  
Ewa Wroblewska-Czajka ◽  
Maria Grolik ◽  
...  
Keyword(s):  
Visual Acuity ◽  
Oral Mucosa ◽  
Amniotic Membrane ◽  
Single Cells ◽  
Autologous Transplantation ◽  
Visual Axis ◽  
Corneal Surface ◽  
Ocular Surface Reconstruction ◽  
3T3 Fibroblasts ◽  
Mucosa Epithelium

Purpose. Efficacy of cultivated oral mucosa epithelial transplantation (COMET) procedure in corneal epithelium restoration of aniridia patients.Methods. Study subjects were aniridia patients (13 patients; 17 eyes) with irregular, vascular conjunctival pannus involving visual axis who underwent autologous transplantation of cultivated epithelium. For the procedure oral mucosa epithelial cells were obtained from buccal mucosa with further enzymatic treatment. Suspension of single cells was seeded on previously prepared denuded amniotic membrane. Cultures were carried on culture dishes inserts in the presence of the inactivated with Mitomycin C monolayer of 3T3 fibroblasts. Cultures were carried for seven days. Stratified oral mucosa epithelium with its amniotic membrane carrier was transplanted on the surgically denuded corneal surface of aniridia patients with total or subtotal limbal stem cell deficiency.Outcome Measures. Corneal surface, epithelial regularity, and visual acuity improvement were evaluated.Results. At the end of the observation period, 76.4% of the eyes had regular transparent epithelium and 23.5% had developed epithelial defects or central corneal haze; in 88.2% of cases visual acuity had increased. VA range was from HM 0.05 before the surgery to HM up to 0.1 after surgery.Conclusion. Application of cultivated oral mucosa epithelium restores regular epithelium on the corneal surface with moderate improvement in quality of vision.

scholarly journals Anatomy of Shuck Abscission in `Desirable' Pecan

1995 ◽  
Vol 120 (5) ◽  
pp. 790-797 ◽  
Author(s):  
Darrell Sparks ◽  
I.E. Yates
Keyword(s):  
Vascular System ◽  
Cell Types ◽  
Tissue Type ◽  
Cellular Changes ◽  
Maturation Rate ◽  
Electron And Light Microscopy ◽  
Three Stages ◽  
Cellular Modifications ◽  
System Separation ◽  
Tissue Zone

Cellular changes associated with shuck dehiscence and markings deposited on pecan [Carya illinoinensis (Wangenh.) C. Koch] shells were examined by scanning electron and light microscopy. Fruit were sampled at three stages of maturity: 1) shuck and shell fused, 2) sutures separated (shuck opening), and 3) vascular system separated from shuck. Shuck dehiscence involved temporally regulated abscission events with shuck-shell, then shuck-suture, and finally shuck-vascular system separation. Abscission events occurred in a tissue zone common to and continuous among all three separation sites, even though segregated in time. Also, similar cell types and cellular changes were common to the three events. Thus, temporal segregation of abscission events was not due to differences in either tissue type or cellular modifications, but to maturation rate. Structures to become shell markings were single globules filling cells of the shuck inner tissue zone before shuck-shell separation. These globules were deposited on the shell at shuck-shell separation and were morphologically similar to deposits stuck to the dorsal shuck surface. Globules were partitioned differentially between the shuck and shell during shuck-shell separation. Thus, the inner zone of the shuck is an important tissue in pecan nut maturation; it functions as the site for dehiscence and provides markers for cultivar identification.

scholarly journals The Potential of Light Microscopic Features of the Oral Mucosa in Predicting Post-mortem Interval

2021 ◽  
Vol 21 (1) ◽  
pp. e34-41
Author(s):  
Jagganath Patro ◽  
Swagatika Panda ◽  
Neeta Mohanty ◽  
Uma S. Mishra
Keyword(s):  
Connective Tissue ◽  
Oral Mucosa ◽  
Buccal Mucosa ◽  
Periodic Acid ◽  
Post Mortem ◽  
Periodic Acid Schiff ◽  
Post Mortem Interval ◽  
Cellular Changes ◽  
Microscopic Evaluation ◽  
Microscopic Features

Objectives: The post-mortem interval (PMI) refers to the amount of time elapsed between death and discovery of the body. This study aimed to evaluate light microscopic cellular changes in the oral mucosa and identify the potential of this method for predicting PMI. Methods: This prospective study was conducted between July 2016 and January 2018 at the Institute of Dental Sciences, Siksha ‘O’ Anusandhan University, Bhubaneswar, India. A total of 150 post-mortem (including 75 gingival and 75 buccal mucosa samples) and 40 ante-mortem (including 20 gingival and 20 buccal mucosa samples) tissue samples were compared using haematoxylin and eosin, periodic acid-Schiff (PAS) and van Gieson stains. Microscopic changes in the epithelium and connective tissue were categorised according to PMI stage as early (<12.5 hours since death), intermediate (12.5–20.5 hours since death) or late (>20.5 hours since death). Results: Most epithelial cellular changes occurred early, except for arc-shaped nuclei and epithelial shredding which were intermediate and late changes, respectively. However, microscopic changes in the connective tissue were only observable at ≥12.5 hours. There was a progressive decrease in intensity in van Gieson stains and an increase in intensity in PAS stains as PMI increased. Several microscopic features were found to be significant predictors of PMI including epithelial homogenisation, cytoplasmic vacuolation, nuclear degeneration, arc-shaped nuclei, chromatin clumping, red blood cell clumping and lysis, melanin incontinency, myofibril degeneration, salivary gland acini degeneration and epithelial connective tissue separation (P <0.050 each). Conclusion: These findings indicate that microscopic evaluation of the oral mucosa may be helpful for PMI prediction.   KEYWORDS Post-mortem Changes; Light Microscopy; Oral Mucosa; Epithelial Cells; Lamina Propria; Salivary Glands; Histocytochemistry; Periodic Acid-Schiff Reaction; India.

scholarly journals Neighborhood Care and Neighborhood Bonds: An Unequal Relationship

2020 ◽  
pp. 001391652093745
Author(s):  
M. Carmen Hidalgo ◽  
Pilar Moreno-Jiménez ◽  
Gabriel Muiños ◽  
Bernardo Hernández
Keyword(s):  
Social Norms ◽  
Positive Relationship ◽  
Environmental Psychology ◽  
Place Identity ◽  
Urban Environments ◽  
Residential Satisfaction ◽  
Sociodemographic Characteristics ◽  
Natural Environments ◽  
And Behavior ◽  
The Relationship

Research in environmental psychology has found a positive relationship between place bonds and behaviors related to care and maintenance of place. Although this relationship has been analyzed in natural environments, it has been less frequently studied in urban environments and has yielded contradictory results. The aim of this study is to analyze behavior related to care and conservation of neighborhood and its possible relationship to place bonds, as well as to other variables that we think may be important in explaining this behavior. The participants were 407 residents from eight different neighborhoods with different sociodemographic characteristics in one Spanish city. The results indicate that the relationship between attachment and behavior is significant only in residents with higher socioeconomic levels. These findings may help to explain the contradictory results found in the literature. Other variables which are significant in explaining neighborhood care are social norms, residential satisfaction, and support for protection policies. Place identity was not found to be significantly correlated with neighborhood care.

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