scholarly journals Comparing the Efficacy of Toluidine Blue, Methylene Blue and Curcumin in Photodynamic Therapy Against Enterococcus faecalis

2020 ◽  
Vol 11 (Suppl 1) ◽  
pp. S49-S54
Author(s):  
Mohammad Ali Mozayeni ◽  
Farzaneh Vatandoost ◽  
Mohammad Asnaashari ◽  
Mehdi Shokri ◽  
Saranaz Azari-Marhabi ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Photodynamic Therapy ◽  
Enterococcus Faecalis ◽  
Toluidine Blue ◽  
Blue Methylene

scholarly journals IN VITRO EVALUATION OF ANTIMICROBIAL EFFICACY OF DIODE LASER WHEN USED ADJUNCTIVELY WITH SODIUM HYPOCHLORITE, METHYLENE BLUE OR TOLUIDINE BLUE IN ENTEROCOCCUS FAECALIS CONTAMINATED ROOT CANALS

2020 ◽  
pp. 148-151
Author(s):  
N. Shubhashini ◽  
Vinaya Kumar R ◽  
Annapoorna Kini ◽  
Swetha H.B. ◽  
Srikanth Choudhary B.S. ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Diode Laser ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Toluidine Blue ◽  
Antimicrobial Effect ◽  
Positive Control ◽  
Negative Control ◽  
Root Canals

AIM: To evaluate antimicrobial effect of diode laser when used adjunctively with sodium hypochlorite, methylene blue or toluidine blue in Enterococcus faecalis inoculated root canals. METHODOLOGY: 70 extracted uniradicular premolars were prepared with ProTaper files and sterilized. 5 were kept aside as negative control (G1) and the remaining 65 were inoculated with Enterococcus faecalis and incubated for 7 days. Following this, 5 were kept as positive control (G2) and the other 60 were randomly distributed into six groups: G3, diode laser irradiation (1.5W) with sodium hypochlorite; G4, diode laser (3W) with sodium hypochlorite; G5, diode laser (1.5W) with methylene blue; G6, diode laser (3W) with methylene blue; G7, diode laser (1.5W) with toluidine blue; G8, diode laser (3W) with toluidine blue. Subsequently, turbidity was assessed and CFU count determined following intracanal sampling and plating. RESULTS: G4 exhibited significantly lower mean CFUs/mL (P˂0.001) among the experimental groups. G4 demonstrated the most (98.8%) while G7 exhibited the least antibacterial effect (28.66%). Highest turbidity was observed in G5 (0.1310) while the lowest was noted in G4 (0.0287). CONCLUSION: Diode laser used adjunctively with sodium hypochlorite exhibited superior antibacterial efficacy when compared to its use with photosensitizer dyes like methylene blue and toluidine blue.

Comparison of the efficiency of rose bengal and methylene blue as photosensitizers in photodynamic therapy techniques aiming at Enterococcus faecalis inactivation

2015 ◽  
Vol 12 (3) ◽  
pp. 355
Author(s):  
Catia Cilene Nass Sebrão ◽  
Arandi Ginane Bezerra Junior ◽  
Paulo Henrique Condeixa de França ◽  
Leslie Ecker Ferreira ◽  
Vania Portela Ditzel Westphalen
Keyword(s):  
Methylene Blue ◽  
Photodynamic Therapy ◽  
Enterococcus Faecalis ◽  
Rose Bengal

scholarly journals Manual and Rotary Instrumentation Ability to Reduce Enterococcus faecalis Associated with Photodynamic Therapy in Deciduous Molars

2014 ◽  
Vol 25 (6) ◽  
pp. 502-507 ◽  
Author(s):  
Sérgio Luiz Pinheiro ◽  
Josianne Neres da Silva ◽  
Rafael Orro Gonçalves ◽  
Karina Teixeira Villalpando
Keyword(s):  
Photodynamic Therapy ◽  
Enterococcus Faecalis ◽  
Toluidine Blue ◽  
Wilcoxon Test ◽  
Root Canals ◽  
Halogen Light ◽  
Working Length ◽  
Before And After ◽  
Peptone Water ◽  
Deciduous Molars

This aim of this study was to assess the ability of manual or rotary instrumentation associated with photodynamic therapy (PDT) to reduce Enterococcus faecalis using three combinations of light/photosensitizers: toluidine blue O/laser, fuchsin/halogen light and fuchsin/LED. Twenty deciduous molars were selected and contaminated with Enterococcus faecalis (McFarland 0.5 scale). Working length determination was performed by visual method. The teeth were randomly divided into two groups: G1 (n=10): manual instrumentation (Kerr-type files) and G2 (n=10): rotary instrumentation (ProTaper system). The bacteria were collected three times using sterile paper cones compatible with the anatomic diameter of the root canal for 30 s before and after instrumentation and after PDT. The samples were diluted in peptone water, seeded on blood agar plates and incubated in an oven at 37 °C for colony-forming units counting. The decrease of E. faecalis counts after instrumentation and after PDT was compared using the Wilcoxon test, t-test and Kruskal Wallis test. A significant reduction of E. faecalis occurred after manual and rotary instrumentation and after PDT using the three combinations of light/photosensitizer (p<0.05). It may be concluded that both rotary and manual instrumentation reduced E. faecalis. Fuchsin with halogen light or LED irradiation and toluidine blue O with laser irradiation can be used to reduce E. faecalis in root canals of primary molars. PDT can be used as an adjuvant to conventional endodontic treatment.

Evaluation of antimicrobial photodynamic therapy with toluidine blue against Enterococcus faecalis: Laser vs LED

2020 ◽  
Vol 32 ◽  
pp. 102036
Author(s):  
Farzaneh Afkhami ◽  
Mahsa Karimi ◽  
Abbas Bahador ◽  
Paniz Ahmadi ◽  
Maryam Pourhajibagher ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Enterococcus Faecalis ◽  
Toluidine Blue ◽  
Antimicrobial Photodynamic Therapy

scholarly journals Comparative in vitro study of intracanal Enterococcus faecalis reduction using photosensitizers in aPDT

2021 ◽  
Vol 20 ◽  
pp. e214034
Author(s):  
Maria Cleide Azevedo Braz ◽  
André Hayato Saguchi ◽  
Eduardo Akisue ◽  
Adriana de Oliveira Lira ◽  
Sidnea Aparecida Freitas Paiva ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Confidence Interval ◽  
Enterococcus Faecalis ◽  
Toluidine Blue ◽  
Irradiation Time ◽  
In Vitro Study ◽  
Human Teeth ◽  
Significant Difference ◽  
Group Comparisons

Aim: To compare Enterococcus faecalis reduction after antimicrobial photodynamic therapy (aPDT) used with methylene blue, toluidine blue, tannin, and curcumin as photosensitizers, an adjunct to endodontic chemomechanical preparation (CMP) in root canals of human teeth. Methods: A total of 120 single-rooted teeth were divided into 6 groups (n  =  20): G1- CMP and 2.5% sodium hypochlorite (NaOCl); G2- CMP and saline solution; G3- CMP, 2.5% NaOCl, and aPDT with 0.005% methylene blue; G4- CMP, 2.5% NaOCl, and aPDT with 0.005% toluidine blue; G5- CMP, 2.5% NaOCl, and aPDT with 0.005% tannin; and G6- CMP, 2.5% NaOCl, and aPDT with 0.005% curcumin. A portable semiconductor laser was used (660  nm, 100  mW, 1.8  J, 180s) in groups 1, 2, 3, 4, 5, and a blue LED light-curing (420-480  nm, 1200 mV/cm2 ) in G6. For all groups, a 5 min pre-irradiation time was applied. Samples were collected before (initial collection), immediately after (intermediate collection) and 7 days after CMP (final collection) for colony-forming unit (CFU) counting. The Kruskal-Wallis test and analysis of variance (ANOVA) were performed (p < 0.05; 95% confidence interval). Results: In between-group comparisons, there was no significant difference observed in the number of CFUs at the initial (p >< 0.001) and final collections (p >< 0.001) for G2 and G3. In within-group comparisons, the number of CFUs showed a decreasing trend in G4 (p = 0.007) and G5 (p = 0.001). Conclusion: Photosensitizers promoted E. faecalis reduction, with better results for tannin and curcumin. Alternative photosensitizers should be the focus of further studies.>< 0.05; 95% confidence interval). Results: In between-group comparisons, there was no significant difference observed in the number of CFUs at the initial (p < 0.001) and final collections (p >< 0.001) for G2 and G3. In within-group comparisons, the number of CFUs showed a decreasing trend in G4 (p = 0.007) and G5 (p = 0.001). Conclusion: Photosensitizers promoted E. faecalis reduction, with better results for tannin and curcumin. Alternative photosensitizers should be the focus of further studies.><  0.001) and final collections (p < 0.001) for G2 and G3. In within-group comparisons, the number of CFUs showed a decreasing trend in G4 (p = 0.007) and G5 (p = 0.001). Conclusion: Photosensitizers promoted E. faecalis reduction, with better results for tannin and curcumin. Alternative photosensitizers should be the focus of further studies>< 0.001) for G2 and G3. In within-group comparisons, the number of CFUs showed a decreasing trend in G4 (p = 0.007) and G5 (p = 0.001). Conclusion: Photosensitizers promoted E. faecalis reduction, with better results for tannin and curcumin. Alternative photosensitizers should be the focus of further studies.

Influence of light intensity and irradiation mode on methylene blue, chlorin-e6 and curcumin-mediated photodynamic therapy against Enterococcus faecalis

2020 ◽  
Vol 31 ◽  
pp. 101925
Author(s):  
Laís Simões Sampaio ◽  
Sarah Raquel de Annunzio ◽  
Laura Marise de Freitas ◽  
Lívia Oliveira Dantas ◽  
Leonardo de Boni ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Photodynamic Therapy ◽  
Light Intensity ◽  
Enterococcus Faecalis ◽  
Chlorin E6

Effect of Photodynamic Therapy with Different Formulations of Methylene Blue in Teeth Contaminated by Enterococcus faecalis

2013 ◽  
Vol 03 (04) ◽  
Author(s):  
Eliana Barbosa de Souza Maria Regina Lorenzetti Simionato
Keyword(s):  
Methylene Blue ◽  
Photodynamic Therapy ◽  
Enterococcus Faecalis
Sign in / Sign up

Export Citation Format

Share Document