Role of B. Licheniformis in bio mineralization of calcium carbonate and its biological applications

Bio mineralization is a significant process carried out by living organisms in which minerals are produced through the hardening of biological tissues. Herein, the current study focus on calcium carbonate precipitation, as part of bio mineralization, to be used in applications for CO2 sequestration, material technology, and other fields. A strain B. licheniformis, isolated from marine water, was investigated for its ability to produce urease and induce calcium carbonate precipitation in a metabolic process. It was discovered that B. licheniformis, resisted high concentrations of urea up to 60 g/L. In order to optimize the calcification process of B. licheniformis, the Calcium carbonate precipitation media is used respectively, pH of 10, and culture time of 96 h. Using X-ray diffraction and Scanning Electron Microscopy analysis, the calcium carbonate polymorphs produced by B. licheniformis, were proven to be mainly calcite. The results of this research provide evidence that B. licheniformis can biologically induce calcification and suggest that B. licheniformis may play a potential role in the synthesis of new bio minerals and in bioremediation or bio recovery.

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Optimization of Calcium Carbonate Precipitation for Bacillus pasteurii

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.178-181.676 ◽

2012 ◽

Vol 178-181 ◽

pp. 676-679 ◽

Cited By ~ 1

Author(s):

Tao Duan ◽

Wen Kun Zhu

Keyword(s):

Calcium Carbonate ◽

Crystal Formation ◽

Carbonate Precipitation ◽

X Ray Diffraction ◽

Calcium Carbonate Precipitation ◽

Electron Microscopic ◽

Bacillus Pasteurii ◽

Scanning Electron Microscopic ◽

External Conditions ◽

Effects Of Temperature

The effects of temperature, pH, precipitation time, reactant concentration, the crystal formation additive on the yield of calcium carbonate precipitation induced by bacillus pasteurii were investigated through orthogonal test. The morphology and structure of the calcium carbonate were characterized by scanning electron microscopic (SEM), Fourier transform infrared spectroscopy (IR) and powder X-ray diffraction (XRD). The results showed that the optimum conditions of calcium carbonate precipitation induced by bacillus pasteurii were temperature of 40oC, pH of 8, precipitation time of 3 d, Ca2+ of 1.5 mol/L, and Mg2+ of 0.05 mol/L. The crystal of calcium carbonate was calcites or mixture of calcites and vaterite. Its morphology and packing density were changed by different external conditions.

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Role of Na-Montmorillonite on Microbially Induced Calcium Carbonate Precipitation

Molecules ◽

10.3390/molecules26206211 ◽

2021 ◽

Vol 26 (20) ◽

pp. 6211

Author(s):

Guowang Tang ◽

Cangqin Jia ◽

Guihe Wang ◽

Peizhi Yu ◽

Haonan Zhang

Keyword(s):

Calcium Carbonate ◽

Sandy Soil ◽

Carbonate Precipitation ◽

Soil Consolidation ◽

X Ray Diffraction ◽

Calcium Carbonate Precipitation ◽

X Ray ◽

Significant Attention ◽

Previous Reaction

The use of additives has generated significant attention due to their extensive application in the microbially induced calcium carbonate precipitation (MICP) process. This study aims to discuss the effects of Na-montmorillonite (Na-MMT) on CaCO3 crystallization and sandy soil consolidation through the MICP process. Compared with the traditional MICP method, a larger amount of CaCO3 precipitate was obtained. Moreover, the reaction of Ca2+ ions was accelerated, and bacteria were absorbed by a small amount of Na-MMT. Meanwhile, an increase in the total cementing solution (TCS) was not conducive to the previous reaction. This problem was solved by conducting the reaction with Na-MMT. The polymorphs and morphologies of the CaCO3 precipitates were tested by using X-ray diffraction and scanning electron microscopy. Further, when Na-MMT was used, the morphology of CaCO3 changed from an individual precipitate to agglomerations of the precipitate. Compared to the experiments without Na-MMT in the MICP process, the addition of Na-MMT significantly reduced the hydraulic conductivity (HC) of sandy soil consolidated.

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Microbiologically Induced Carbonate Precipitation in the Restoration and Conservation of Cultural Heritage Materials

Molecules ◽

10.3390/molecules25235499 ◽

2020 ◽

Vol 25 (23) ◽

pp. 5499

Author(s):

Erick Ortega-Villamagua ◽

Marco Gudiño-Gomezjurado ◽

Alex Palma-Cando

Keyword(s):

Calcium Carbonate ◽

Carbonate Precipitation ◽

High Concentration ◽

Calcium Carbonate Precipitation ◽

Bacterial Surface ◽

Historical Materials ◽

Nucleation Sites ◽

Environmental Agents ◽

Living Organisms ◽

Clay Rocks

Microbiologically induced carbonate precipitation (MICP) is a well-known biogeochemical process that allows the formation of calcium carbonate deposits in the extracellular environment. The high concentration of carbonate and calcium ions on the bacterial surface, which serves as nucleation sites, promotes the calcium carbonate precipitation filling and binding deteriorated materials. Historic buildings and artwork, especially those present in open sites, are susceptible to enhanced weathering resulting from environmental agents, interaction with physical-chemical pollutants, and living organisms, among others. In this work, some published variations of a novel and ecological surface treatment of heritage structures based on MICP are presented and compared. This method has shown to be successful as a restoration, consolidation, and conservation tool for improvement of mechanical properties and prevention of unwanted gas and fluid migration from historical materials. The treatment has revealed best results on porous media matrixes; nevertheless, it can also be applied on soil, marble, concrete, clay, rocks, and limestone. MICP is proposed as a potentially safe and powerful procedure for efficient conservation of worldwide heritage structures.

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