Optimization of Calcium Carbonate Precipitation for Bacillus pasteurii

2012 ◽  
Vol 178-181 ◽  
pp. 676-679 ◽  
Author(s):  
Tao Duan ◽  
Wen Kun Zhu
Keyword(s):  
Calcium Carbonate ◽  
Crystal Formation ◽  
Carbonate Precipitation ◽  
X Ray Diffraction ◽  
Calcium Carbonate Precipitation ◽  
Electron Microscopic ◽  
Bacillus Pasteurii ◽  
Scanning Electron Microscopic ◽  
External Conditions ◽  
Effects Of Temperature

The effects of temperature, pH, precipitation time, reactant concentration, the crystal formation additive on the yield of calcium carbonate precipitation induced by bacillus pasteurii were investigated through orthogonal test. The morphology and structure of the calcium carbonate were characterized by scanning electron microscopic (SEM), Fourier transform infrared spectroscopy (IR) and powder X-ray diffraction (XRD). The results showed that the optimum conditions of calcium carbonate precipitation induced by bacillus pasteurii were temperature of 40oC, pH of 8, precipitation time of 3 d, Ca2+ of 1.5 mol/L, and Mg2+ of 0.05 mol/L. The crystal of calcium carbonate was calcites or mixture of calcites and vaterite. Its morphology and packing density were changed by different external conditions.

Effects of Various Sugars on the Mineralization of Microbiologically Induced Calcium Carbonate

2010 ◽  
Vol 152-153 ◽  
pp. 1669-1672
Author(s):  
Wen Kun Zhu ◽  
Xue Gang Luo
Keyword(s):  
Calcium Carbonate ◽  
Culture Medium ◽  
Precipitation Amount ◽  
Carbonate Precipitation ◽  
Calcium Carbonate Precipitation ◽  
Bacillus Pasteurii

Sugar is one of the key nutrients required to microorganism growth. In this paper , the effects of various sugars on the mineralization of calcium carbonate induced by Bacillus pasteurii . The precipitation amount, forms and structures of calcium carbonate were characterized. The results indicated that the induced calcium carbonate precipitation are mainly calcite as irregular aggregated bodies and rhombus blocks and aragonite appeared when the culture medium was added with dextran. The calcium carbonate precipitation reached a maximum of 2.32 g when glucose was added into the culture.

scholarly journals Strain-Specific Ureolytic Microbial Calcium Carbonate Precipitation

2003 ◽  
Vol 69 (8) ◽  
pp. 4901-4909 ◽  
Author(s):  
Frederik Hammes ◽  
Nico Boon ◽  
Johan de Villiers ◽  
Willy Verstraete ◽  
Steven Douglas Siciliano
Keyword(s):  
Calcium Carbonate ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Gene Diversity ◽  
Bacillus Sphaericus ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Crystal Formation ◽  
Carbonate Precipitation ◽  
Calcium Carbonate Precipitation ◽  
Gradient Gel Electrophoresis

ABSTRACT During a study of ureolytic microbial calcium carbonate (CaCO3) precipitation by bacterial isolates collected from different environmental samples, morphological differences were observed in the large CaCO3 crystal aggregates precipitated within bacterial colonies grown on agar. Based on these differences, 12 isolates were selected for further study. We hypothesized that the striking differences in crystal morphology were the result of different microbial species or, alternatively, differences in the functional attributes of the isolates selected. Sequencing of 16S rRNA genes showed that all of the isolates were phylogenetically closely related to the Bacillus sphaericus group. Urease gene diversity among the isolates was examined by using a novel application of PCR-denaturing gradient gel electrophoresis (DGGE). This approach revealed significant differences between the isolates. Moreover, for several isolates, multiple bands appeared on the DGGE gels, suggesting the apparent presence of different urease genes in these isolates. The substrate affinities (Km ) and maximum hydrolysis rates (V max) of crude enzyme extracts differed considerably for the different strains. For certain isolates, the urease activity increased up to 10-fold in the presence of 30 mM calcium, and apparently this contributed to the characteristic crystal formation by these isolates. We show that strain-specific calcification occurred during ureolytic microbial carbonate precipitation. The specificity was mainly due to differences in urease expression and the response to calcium.

scholarly journals Role of Na-Montmorillonite on Microbially Induced Calcium Carbonate Precipitation

Molecules ◽  
2021 ◽  
Vol 26 (20) ◽  
pp. 6211
Author(s):  
Guowang Tang ◽  
Cangqin Jia ◽  
Guihe Wang ◽  
Peizhi Yu ◽  
Haonan Zhang
Keyword(s):  
Calcium Carbonate ◽  
Sandy Soil ◽  
Carbonate Precipitation ◽  
Soil Consolidation ◽  
X Ray Diffraction ◽  
Calcium Carbonate Precipitation ◽  
X Ray ◽  
Significant Attention ◽  
Previous Reaction

The use of additives has generated significant attention due to their extensive application in the microbially induced calcium carbonate precipitation (MICP) process. This study aims to discuss the effects of Na-montmorillonite (Na-MMT) on CaCO3 crystallization and sandy soil consolidation through the MICP process. Compared with the traditional MICP method, a larger amount of CaCO3 precipitate was obtained. Moreover, the reaction of Ca2+ ions was accelerated, and bacteria were absorbed by a small amount of Na-MMT. Meanwhile, an increase in the total cementing solution (TCS) was not conducive to the previous reaction. This problem was solved by conducting the reaction with Na-MMT. The polymorphs and morphologies of the CaCO3 precipitates were tested by using X-ray diffraction and scanning electron microscopy. Further, when Na-MMT was used, the morphology of CaCO3 changed from an individual precipitate to agglomerations of the precipitate. Compared to the experiments without Na-MMT in the MICP process, the addition of Na-MMT significantly reduced the hydraulic conductivity (HC) of sandy soil consolidated.

Role of B. Licheniformis in bio mineralization of calcium carbonate and its biological applications

2019 ◽  
Vol 1 (6) ◽  
pp. 622-629
Author(s):  
Balakrishnan Arumugam ◽  
Brinda Elangovan
Keyword(s):  
Calcium Carbonate ◽  
Biological Tissues ◽  
Carbonate Precipitation ◽  
X Ray Diffraction ◽  
Calcium Carbonate Precipitation ◽  
Material Technology ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis ◽  
High Concentrations ◽  
Living Organisms

Bio mineralization is a significant process carried out by living organisms in which minerals are produced through the hardening of biological tissues. Herein, the current study focus on calcium carbonate precipitation, as part of bio mineralization, to be used in applications for CO2 sequestration, material technology, and other fields. A strain B. licheniformis, isolated from marine water, was investigated for its ability to produce urease and induce calcium carbonate precipitation in a metabolic process. It was discovered that B. licheniformis, resisted high concentrations of urea up to 60 g/L. In order to optimize the calcification process of B. licheniformis, the Calcium carbonate precipitation media is used respectively, pH of 10, and culture time of 96 h. Using X-ray diffraction and Scanning Electron Microscopy analysis, the calcium carbonate polymorphs produced by B. licheniformis, were proven to be mainly calcite. The results of this research provide evidence that B. licheniformis can biologically induce calcification and suggest that B. licheniformis may play a potential role in the synthesis of new bio minerals and in bioremediation or bio recovery.

scholarly journals Bioprecipitation of Calcium Carbonate Crystals by Bacteria Isolated from Saline Environments Grown in Culture Media Amended with Seawater and Real Brine

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
G. A. Silva-Castro ◽  
I. Uad ◽  
A. Gonzalez-Martinez ◽  
A. Rivadeneyra ◽  
J. Gonzalez-Lopez ◽  
...  
Keyword(s):  
Organic Matter ◽  
Calcium Carbonate ◽  
Sea Water ◽  
Culture Media ◽  
Calcium Sulphate ◽  
Carbonic Anhydrase Activity ◽  
Rrna Gene ◽  
Crystal Formation ◽  
Carbonate Precipitation ◽  
Calcium Carbonate Precipitation

The precipitation of calcium carbonate and calcium sulphate by isolated bacteria from seawater and real brine obtained in a desalination plant growth in culture media containing seawater and brine as mineral sources has been studied. However, only bioprecipitation was detected when the bacteria were grown in media with added organic matter. Biomineralization process started rapidly, crystal formation taking place in the beginning a few days after inoculation of media; roughly 90% of total cultivated bacteria showed. Six major colonies with carbonate precipitation capacity dominated bacterial community structure cultivated in heterotrophic platable bacteria medium. Taxonomic identification of these six strains through partial 16S rRNA gene sequences showed their affiliation with Gram-positiveBacillusandVirgibacillusgenera. These strains were able to form calcium carbonate minerals, which precipitated as calcite and aragonite crystals and showed bacterial fingerprints or bacteria calcification. Also, carbonic anhydrase activity was observed in three of these isolated bacteria. The results of this research suggest that microbiota isolated from sea water and brine is capable of precipitation of carbonate biominerals, which can occurin situwith mediation of organic matter concentrations. Moreover, calcium carbonate precipitation ability of this microbiota could be of importance in bioremediation of CO2and calcium in certain environments.

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