PENGARUH EKSTRAK Tagetes erecta L TERHADAP PERTUMBUHAN JAMUR Saprolegnia sp SECARA IN VITRO

Saprolegnia sp fungi are freshwater mushrooms that live in freshwater environments and require water to grow and reproduce. Mushroom Saprolegnia is a major type of water fungus that is associated with fungal infections of fish and eggs that are in freshwater. Saprolegnia sp fungus is also called a cold water fungus because it spreads in cold water. Saprolegnia fungus growth in fish / egg bodies or suitable substrate is affected by water temperature. Saprolegnia sp attack causes clinical symptoms that are filled with white threads such as cotton grown in fish eggs. To overcome this problem needed a solution for the prevention of fungal infections one of them is by using anti-fungal herbs such as chicken shoot leaves (Tagetes erecta L). therefore this research needs to be done to control fungal infections Saprolegnia sp on tawes fish eggs. This study used a complete randomized design (RAL), each treatment was repeated three times, the treatment was: P0 = ppm, P1 = 400 ppm, P2 = 600 ppm, and P3 = 800 ppm. The results showed that the most effective extraction was found in treatment P1 with an average value of 14%.

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PENGENDALIAN JAMUR Saprolegnia sp PADA TELUR IKAN TAWES (Puntius javanicus) MENGGUNAKAN EKSTRAK DAUN BUNGA TAHI AYAM (Tagetes erecta L)

JURNAL PERIKANAN TROPIS ◽

10.35308/jpt.v4i2.784 ◽

2017 ◽

Vol 4 (2) ◽

pp. 101

Author(s):

Farah Diana ◽

Shela Rahmita ◽

Sufal Diansyah

Keyword(s):

Water Quality ◽

Fungal Infection ◽

Fungal Infections ◽

Economic Value ◽

Development Stage ◽

Control Treatment ◽

Tagetes Erecta ◽

Fish Eggs ◽

Fish Breeding ◽

Tagetes Erecta L

Tawes (Puntius javanicus) is one of the freshwater fish species that has economic value in Aceh. One of the obstacle in tawes fish breeding is Saprolegnia sp. fungus that attacks tawes fish eggs. The necessary solutions was needed to resolve fungal infection by using Tagetes erecta L. Therefore this research was very important to control fungal infections on fish eggs Saprolegnia sp.. This study used Randomized Complete Design with four treatments and three raplications for each treatments. The treatment in this study namely P0 = P1 = ppm, 40 ppm, P2 = 50 ppm, P3 = 60 ppm, and P4 = 70 ppm. The parameters observed in this research is the prevalence of eggs hatchability rate, eggs growth and water quality. The results of this study showed that the highest value of tawes eggs hatchability (prevalence) found in treatment 3 (P3 = 60 ppm) was 53.33% and the lowest value was present in control treatment (P0 = 0 ppm) was 36.66%. The reasults of eggs hatchability rate (HR) show the highest values found in treatment 2 (P2 = 50 ppm) of 95.66% and the lowest value is present on the control (P0 = 0 ppm) of 45.66%. On the egg development stage shows that there is a embryogenesis process starts from cleavage, morula, blastula, gastrula, organogenesis to become larvae. Water quality during the research shows that water temperature range were 29.2-30oC, and DO water range were 3.6-4.8 mg/l.

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Proliferasi Tunas Adventif Tagetes (Tagetes erecta L.) Kultivar African Crackerjack dengan BAP, GA3, dan IAA Secara In Vitro

Buletin Agrohorti ◽

10.29244/agrob.5.1.55-68 ◽

2017 ◽

Vol 5 (1) ◽

pp. 55 ◽

Cited By ~ 1

Author(s):

Muhammad Baidowi ◽

Ni Made Armini Wiendi

Keyword(s):

Tagetes Erecta ◽

Tagetes Erecta L

Tujuan penelitian ini mempelajari proliferasi tunas adventif eksplan hipokotil, epikotil, dan kotiledon Marigold (Tagetes erecta L.) secara in vitro dengan kombinasi BAP, GA3, dan IAA. Percobaan pertama adalah mengenai sterilisasi benih. Eksplan percobaan dua dan tiga adalah epikotil, hipokotil, dan kotiledon dari kecambah aseptik. Eksplan epikotil berhasil terproliferasi sedangkan hipokotil dan kotiledon tidak terproliferasi karena oksidasi fenol. Interaksi BAP dan IAA berpengaruh nyata pada induksi kalus epikotil pada semua minggu pengamatan, sedangkan interaksi BAP dan GA3 tidak berpengaruh nyata pada semua parameter. Komposisi media MS ditambah IAA dan BAP berhasil menginduksi kalus 70.7% dari eksplan epikotoil, 49.3% dari eksplan kotiledon, 53% dari hipokotil. Komposisi media MS ditambah GA3 dan BAP berhasil menginduksi kalus 60% dari eksplan epikotoil, 48% dari eksplan kotiledon, 46% dari hipokotil. Pada percobaan dua, media MS dengan 0.87 mg L-1 IAA + 0.5 mg L-1 BAP dan media MS dengan 0.43 mg L-1IAA + 0.5 mg L-1BAP terbaik dalam menginduksi tunas adventif eksplan epikotil. Pada percobaan tiga, proliferasi tunas adventif tertinggi pada media MS dengan 1.73 mg L-1GA3 + 1.5 mg L-1 BAP. IAA berpengaruh nyata pada eksplan bertunas pada satu 1 MSP, tunas per eksplan pada 3 MSP, dan daun pertunas pada 4 MSP dan 5 MSP, sedangkan BAP berpengaruh nyata pada induksi kalus pada 1 MSP, eksplan bertunas pada 3 MSP, dan tunas per eksplan pada 3 MSP. Pada percobaan tiga, GA3 berpengaruh nyata pada pembentukan akar pada 4 MSP dan 5 MSP, sedangkan BAP berpengaruh nyata pada proliferasi tunas per eksplan pada 3 MSP.

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Effect of Surface Sterilization of Tagetes erecta L. Cv. Inca Yellow Hybrid and Orange Hybrid Nodal Explant on Aseptic in vitro Propagation

Indian Journal of Pure & Applied Biosciences ◽

10.18782/2582-2845.8502 ◽

2020 ◽

Vol 8 (6) ◽

pp. 618-623

Author(s):

Sonali Das ◽

Keyword(s):

In Vitro Propagation ◽

Tagetes Erecta ◽

Nodal Explant ◽

Surface Sterilization ◽

Tagetes Erecta L ◽

Effect Of Surface

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Screening of marigold (Tagetes erecta L.) cultivars for drought stress based on vegetative and physiological characteristics

International Journal of Food and Allied Sciences ◽

10.21620/ijfaas.2017256-63 ◽

2018 ◽

Vol 3 (2) ◽

pp. 56 ◽

Cited By ~ 1

Author(s):

Adnan Younis ◽

Atif Riaz ◽

Muhammad Qasim ◽

Farakh Mansoor ◽

Faisal Zulfiqar ◽

...

Keyword(s):

Water Stress ◽

Drought Stress ◽

Tagetes Erecta ◽

Drought Treatment ◽

Chlorophyll Contents ◽

Drought Tolerant ◽

Randomized Design ◽

Cultivar Selection ◽

Tagetes Erecta L ◽

Water Scarce

Drought tolerance is an important genotypic character to be exploited for the plant cultivar selection under water deficit conditions. In the recent study, we examined the response of two marigold cultivars (Inca and Bonanza) under different regimes of drought stress. The aim was to determine the best performing cultivar under water/drought stress. Three irrigation treatments include; 4 days (T1), 6 days (T2) and 8 days (T3) in comparison to control 1 day (T0) interval were imposed. Response characters under study were morphological, physiological and anatomical. Complete Randomized Design (CRD) with four replications in two factorial arrangements was followed for experiment layout. The results revealed that increasing water stress adversely affect plant height, in both cultivars. Both cultivars showed a decreasing trend to the number of flowers under water stress. Total chlorophyll contents including a, b were also showed reduction under prolonged drought treatment in both cultivars from (2.7 mg g-1 FW) to (1 mg g-1 FW). Overall, the performance of cultivar (cv.) Inca was satisfactory under water stress regimes. These results are helpful for selecting drought tolerant marigold cultivars in water scarce areas.

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Maternal haploid induction in African marigold (Tagetes erecta L.) through in vitro culture of un-fertilized ovules

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-020-01940-0 ◽

2020 ◽

Vol 143 (3) ◽

pp. 549-564

Author(s):

K. Ravindra Kumar ◽

Kanwar Pal Singh ◽

D. V. S. Raju ◽

Reeta Bhatia ◽

Sapna Panwar

Keyword(s):

In Vitro Culture ◽

Tagetes Erecta ◽

Haploid Induction ◽

African Marigold ◽

Tagetes Erecta L

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PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTI-MICROBIAL ACTIVITY OF ETHANOLIC EXTRACT OF STEMS OF THE PLANT TAGETES ERECTA L. (FAMILY-ASTERACEAE)

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2018v10i4.28468 ◽

2018 ◽

Vol 10 (4) ◽

pp. 64

Author(s):

Jafar Ali ◽

Dibyendu Shil

Keyword(s):

Antimicrobial Activity ◽

Microbial Activity ◽

Ethanolic Extract ◽

Diffusion Method ◽

Tagetes Erecta ◽

Phytochemical Analysis ◽

Standard Drug ◽

Phytochemical Constituents ◽

Tagetes Erecta L

Objective: To estimate the anti-microbial activity of ethanolic extract of Tagetes erecta L. (family-Asteraceae) in association with phytochemical analysis.Methods: The ethanolic extract of the stems of TageteseErecta L. was prepared and investigate for phytochemical constituents using standard methods. Antimicrobial activity of ethanolic extract of has been carried out against one Gram positive bacteria-Staphylococcus aureus and one Gram negative bacteria-Escherichia coli, and the antifungal activity of the plant extract was evaluated on Candida albicans species. The testing was done by the disc diffusion method to visualize the zone of inhibition of ethanolic extract was compared with that of standard drug i.e., Ciprofloxacin for anti-bacterial activity and Terbinafine for anti-fungal activity. The MIC was determined by the microdilution method (Cup-plate method).Results: The present investigation shows the phytochemical analysis, antimicrobial activity of the ethanolic extract of the stems of Tagetes erecta L. Various phytochemical analysis confirmed the presence of alkaloids, saponin glycosides, flavonoids, phenol, proteins and amino acids, cardiac glycosides, steroids, tanin and phenolic compounds. The anti-microbial activity of the ethanolic extract of the plant showed significant results against all three of the test organisms in compare to the standard drugs.Conclusion: The present study concluded that ethanolic extract of-of the stems of Tagetes erecta L. Proved the existence of phytochemical constituents. The ethanolic extract of the plant was found to possess promising antimicrobial activity when compared with the standards and it can be useful for the development of the antibiotics.

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In vitro production of thiophenes using hairy root cultures of Tagetes erecta (L.)

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb2015.14483 ◽

2016 ◽

Vol 15 (17) ◽

pp. 706-713

Author(s):

Gupta Vijayta ◽

Shanker Karuna ◽

ur Rahman Laiq

Keyword(s):

Hairy Root ◽

Tagetes Erecta ◽

Hairy Root Cultures ◽

Root Cultures ◽

In Vitro Production ◽

Tagetes Erecta L ◽

Vitro Production

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Phytochemical Profile, Antioxidant Activity, and Cytotoxicity Assessment of Tagetes erecta L. Flowers

Molecules ◽

10.3390/molecules26051201 ◽

2021 ◽

Vol 26 (5) ◽

pp. 1201

Author(s):

Ana Flavia Burlec ◽

Łukasz Pecio ◽

Solomiia Kozachok ◽

Cornelia Mircea ◽

Andreia Corciovă ◽

...

Keyword(s):

Biological Activities ◽

In Vitro Cytotoxicity ◽

In Vitro Assays ◽

Tagetes Erecta ◽

Phytochemical Analysis ◽

Total Extract ◽

Tagetes Erecta L ◽

Cytotoxicity Assessment

Tagetes erecta L. is a popular ornamental plant of the Asteraceae family, which is widely cultivated not only for its decorative use, but also for the extraction of lutein. Besides carotenoid representatives, which have been extensively studied, other important classes of secondary metabolites present in the plant, such as polyphenols, could exhibit important biological activities. The phytochemical analysis of a methanolic extract obtained from T. erecta inflorescences was achieved using liquid chromatography–mass spectrometry (LC-MS) techniques. The extract was further subjected to a multistep purification process, which allowed the separation of different fractions. The total extract and its fractions contain several polyphenolic compounds, such as hydroxybenzoic and hydroxycinnamic acid derivatives, flavonols (especially quercetagetin glycosides), and several aglycons (e.g., quercetin, patuletin). One of the fractions, containing mostly quercetagitrin, was subjected to two different antioxidant assays (metal chelating activity and lipoxygenase inhibition) and to in vitro cytotoxicity assessment. Generally, the biological assays showed promising results for the investigated fraction compared to the initial extract. Given the encouraging outcome of the in vitro assays, further purification and structural analysis of compounds from T. erecta extracts, as well as further in vivo investigations are justified.

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