scholarly journals UJI SENYAWA FLAVONOID TOTAL DARI EKSTRAK ETANOL HERBA BINARA (Artemisia annua) MENGGUNAKAN HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) TAHUN 2019

2019 ◽  
Vol 2 (1) ◽  
pp. 16-20
Author(s):  
Jhon Patar Sinurat ◽  
Novandi Purba ◽  
Romauli Anna Teresia Marbun
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ethyl Acetate ◽  
High Performance ◽  
Artemisia Annua ◽  
Ethanol Extract ◽  
Partial Structure ◽  
Water Solvent ◽  
Fat Removal ◽  
Two Stages

ABSTRAK Herba binara (Artemisia Annua) is one of the plants that contains flavonoids which are efficacious as antioxidants and anticancer. The purpose of this study was to determine the total flavonoid compounds contained in ethanol extract of binara herbs (Artemisia Annua). Compounds in ethanol extract of binara herbs were identified by phytochemical screening. The results showed that the ethanol extract of binara herbs contained alkaloids, flavonoids, saponins, and tannins. Extraction was carried out in two stages, namely maceration stage with 96% ethanol and fat removal stage by hydrolysis using distilled water solvent. The extract was partitioned with ethyl acetate and n-hexane solvents, then the partitions were examined by TLC using the mobile phase of chloroform and ethyl acetate (7: 3). Ribbon patches that have the same Rf and color prices as the initial detection are taken and searched. Then analyzed using High Performance Liquid Chromatography (HPLC). Based on the wavelength as well as the TLC test, a partial structure which was strongly suspected of total flavonoids was 0.93 g. Kata Kunci :Herba Binara (Artemisia Annua), flavonoid, KLT, High Performance Liquid Chromatography(HPLC).

Standardisation of Artemisia annua using Reversed Phase High Performance Liquid Chromatography (RP-HPLC).

2010 ◽  
Vol 2 (7) ◽  
pp. 142-147
Author(s):  
O. Amos Abolaji ◽  
M. Ubana Eteng ◽  
E. Patrick Ebong ◽  
Andi Brisibe ◽  
Ahmed Shakil ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Artemisia Annua ◽  
Reversed Phase ◽  
Rp Hplc

scholarly journals Phytochemical analysis and antioxidant capacity ofTabernaemontana catharinensis A. DC. Fruits and branches

2014 ◽  
Vol 86 (2) ◽  
pp. 881-888 ◽  
Author(s):  
MARIANA PIANA ◽  
ALINE A. BOLIGON ◽  
THIELE F. DE BRUM ◽  
MARINA ZADRA ◽  
BIANCA V. BELKE ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Chlorogenic Acid ◽  
Condensed Tannins ◽  
High Performance ◽  
Ethyl Acetate Fraction ◽  
Phytochemical Analysis ◽  
Dpph Method

The antioxidant capacity of the crude extract and fractions ofTabernaemontana catharinensis fruits and branches, was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and the content of polyphenols, flavonoids, alkaloids and condensed tannins were determined by the spectrophotometric method. The ethyl acetate fraction of the fruits and the n-butanol fraction of the branches showed IC50 of 181.82 µg/mL and 78.19 µg/mL, respectively. All fractions were analyzed by high performance liquid chromatography (HPLC), in the branches were quantified chlorogenic acid in the chloroform (8.96 mg/g), ethyl acetate (4.31 mg/g) and n-butanol (3.33 mg/g) fractions; caffeic acid in the ethyl acetate (5.24 mg/g) and n-butanol (1.81 mg/g); gallic acid (0.52 mg/g) in the n-butanol. In the fruits, chlorogenic acid in the chloroform (1.67 mg/g); rutin in the ethyl acetate (3.45 mg/g) and n-butanol (8.98 mg/g) fractions. The present study showed that these quantified compounds can contribute to antioxidant capacity which was higher in the branches than in the fruits.

Chromatographic behaviour of some porphyrins and their complexes with Zn, Pd(II) and Cu(II)

2000 ◽  
Vol 04 (02) ◽  
pp. 209-214 ◽  
Author(s):  
M. I. UVAROVA ◽  
G. D. BRYKINA ◽  
O. A. SHPIGUN
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ethyl Acetate ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Zinc Complexes ◽  
Acetate Concentration ◽  
Hydrophobic Compounds ◽  
Retention Parameters

In this work the influence of the porphyrin structure and of the nature of the mobile phase upon retention parameters is examined by means of reversed phase high-performance liquid chromatography (HPLC). Acetonitrile–ethyl acetate and other mixtures were used as eluents. An increase in the retention of azo- and benzosubstituted porphyrins as well as of those containing a large number of carbon atoms as substituents of macrocycles may be noted. A variation in the polarity of the mobile phase affects the retention of the ligands more than that of their zinc complexes. The retention of the most hydrophobic compounds may be well described in coordinates lg k'– lg M. For less hydrophobic porphyrins these dependences are close to linear only within limited intervals of mobile phase ethyl acetate concentration. The best separation of zinc complexes was achieved with acetonitrile as the eluent. The detection limit of porphyrin ligands and complexes with metals is n × 10-8 M.

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