Different species of the Cactaceae family have always been popular as collectible plants. Peter the Great Botanical Garden of the Komarov Botanical Institute holds a representative collection of species from this genus. Of the 185 names adopted according to The Plant List (http://www.theplantlist.org), there are currently about 160 species in the collection of the Garden, plus almost 30 intraspecific taxa. For species that reproduce only by seeds, it is important to study the features of their latent period and evaluate their quality (35 species, 87 accessions). The collection of cacti and succulents of the Peter the Great Botanical garden contains a large number of genera and species as well as forms and varieties from this family. Despite the fact that at present some taxa are united, however, specific grown plants produce seeds of different quality. The results of studying fruits and seeds in a number of species of the genus Mammillaria maintained at Peter the Great Botanical Garden of the Komarov Botanical Institute are presented. The number of seeds in the fruit, the weight of 1000 seeds, and laboratory germination of seeds with different shelf life under laboratory conditions were measured. Seeds were germinated using a standard technique — in Petri dishes on filter paper, without the use of stimulants. It is shown that germination of freshly harvested seeds is slow and stretched in time. Their germination rate does not reach significant performance. The highest germination rate is observed in many species of the genus Mammillaria after 2–3 years of storage. After 5–7 years of storage, the seeds should not be used for exchange between botanical gardens. But anyway, some species (M. flavescens (DC.) DC., M. prolifera subsp. haitiensis (K. Schum.) D. R. Hunt, M. rhodantha Link et Otto) of this genus remain viable for a long time (up to 10 years, as for example, M. mammillaris (L.) Hikers. [=M. simplex Haw.]). At the seed laboratory of the Peter the Great Botanical Garden, the seed storage period for species of the genus Mammillaria suitable for interbotanical exchange is set at a maximum of 7 years, for them to be listed in Index Seminum (or Delectus). Older seeds are used to replenish the carpological collection of the Garden.