scholarly journals Fluorescence intensity changes depending on viscoelasticity of lipid shell coating microbubbles labeled with an indocyanine green derivative

2021 ◽  
Vol 60 (SD) ◽  
pp. SDDE10
Author(s):  
K. Yoshida ◽  
M. Ebata ◽  
C. Kaneko ◽  
Y. Zhang ◽  
Y. Shibata ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Fluorescence Intensity ◽  
Intensity Changes

Near-infrared fluorescence imaging with indocyanine green for quantification of changes in tissue perfusion following revascularization

Vascular ◽  
2021 ◽  
pp. 170853812110328
Author(s):  
Pim Van den Hoven ◽  
Floris S Weller ◽  
Merel Van De Bent ◽  
Lauren N Goncalves ◽  
Melissa Ruig ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Fluorescence Imaging ◽  
Fluorescence Intensity ◽  
Near Infrared ◽  
Tissue Perfusion ◽  
Control Group ◽  
Maximum Slope ◽  
Nir Fluorescence ◽  
Significant Difference ◽  
Revascularization Procedures

Objectives Current diagnostic modalities for patients with peripheral artery disease (PAD) mainly focus on the macrovascular level. For assessment of tissue perfusion, near-infrared (NIR) fluorescence imaging using indocyanine green (ICG) seems promising. In this prospective cohort study, ICG NIR fluorescence imaging was performed pre- and post-revascularization to assess changes in foot perfusion. Methods ICG NIR fluorescence imaging was performed in 36 patients with PAD pre- and post-intervention. After intravenous bolus injection of 0.1 mg/kg ICG, the camera registered the NIR fluorescence intensity over time on the dorsum of the feet for 15 min using the Quest Spectrum Platform®. Time-intensity curves were plotted for three regions of interest (ROI): (1) the dorsum of the foot, (2) the forefoot, and (3) the hallux. Time-intensity curves were normalized for maximum fluorescence intensity. Extracted parameters were the maximum slope, area under the curve (AUC) for the ingress, and the AUC for the egress. The non-treated contralateral leg was used as a control group. Results Successful revascularization was performed in 32 patients. There was a significant increase for the maximum slope and AUC egress in all three ROIs. The most significant difference was seen for the maximum slope in ROI 3 (3.7%/s to 6.6%/s, p < 0.001). In the control group, no significant differences were seen for the maximum slope and AUC egress in all ROIs. Conclusions This study shows the potential of ICG NIR fluorescence imaging in assessing the effect of revascularization procedures on foot perfusion. Future studies should focus on the use of this technique in predicting favorable outcome of revascularization procedures.

scholarly journals Chiral recognition and enantiomer excess determination based on emission wavelength change of AIEgen rotor

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Ming Hu ◽  
Ying-Xue Yuan ◽  
Weizhou Wang ◽  
Dong-Mi Li ◽  
Hong-Chao Zhang ◽  
...  
Keyword(s):  
Carboxylic Acid ◽  
Theoretical Calculation ◽  
Fluorescence Intensity ◽  
Chiral Recognition ◽  
Natural World ◽  
Enantiomer Excess ◽  
Intensity Changes ◽  
Colour Changes ◽  
Chiral Receptors ◽  
Fluorescence Wavelength

AbstractChiral recognition, such as enantioselective interactions of enzyme with chiral agents, is one of the most important issues in the natural world. But artificial chiral receptors are much less efficient than natural ones. For tackling the chiral recognition and enantiomer excess (ee) analysis, up until now all the fluorescent receptors have been developed based on fluorescence intensity changes. Here we report that the chiral recognition of a large number of chiral carboxylic acids, including chiral agrochemicals 2,4-D, is carried out based on fluorescent colour changes rather than intensity changes of AIEgen rotors. Moreover, the fluorescence wavelength of the AIEgen rotor linearly changes with ee of the carboxylic acid, enabling the ee to be accurately measured with average absolute errors (AAE) of less than 2.8%. Theoretical calculation demonstrates that the wavelength change is ascribed to the rotation of the AIEgen rotor upon interaction with different enantiomers.

scholarly journals Two-photon microscopy of cortical NADH fluorescence intensity changes: correcting contamination from the hemodynamic response

2011 ◽  
Vol 16 (10) ◽  
pp. 106003 ◽  
Author(s):  
Edward Baraghis ◽  
Anna Devor ◽  
Qianqian Fang ◽  
Vivek J. Srinivasan ◽  
Weicheng Wu ◽  
...  
Keyword(s):  
Fluorescence Intensity ◽  
Hemodynamic Response ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Nadh Fluorescence ◽  
Intensity Changes

scholarly journals Intraoperative quality assessment of tissue perfusion with indocyanine green (ICG) in a porcine model of mesenteric ischemia

PLoS ONE ◽  
2021 ◽  
Vol 16 (7) ◽  
pp. e0254144
Author(s):  
Anna Duprée ◽  
Henrik Rieß ◽  
Philipp H. von Kroge ◽  
Jakob R. Izbicki ◽  
Eike S. Debus ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Fluorescence Intensity ◽  
Mesenteric Ischemia ◽  
Porcine Model ◽  
Assessment Tool ◽  
Side Branch ◽  
Fluorescent Imaging ◽  
Ischemic Bowel ◽  
Group I ◽  
Observation Period

Background Mesenteric ischemia is a severe and potentially lethal event. Assessment of intestine perfusion is eminently depending on the skills, and the experience of the surgeon. Thus, the therapy is biased by the right evaluation. Aim of this study is to determine the applicability, and the usefulness of fluorescent-imaging (FI) with indocyanine green (ICG) in a porcine model of mesenteric ischemia. Second end-point is the verification of a visual and quantitative assessment tool of the intestinal perfusion. Methods In 18 pigs (54,2 ±2,9kg) an occlusion of a side-branch of the mesenteric artery was performed for 3 (group I, n = 7), 6 (group II, n = 7), and 10 hours (group III, n = 4). After reperfusion a 60 minutes observation period was carried out. 3 regions of interest were defined: ischemic bowel (D1), transitional zone (D2), and non-ischemic bowel (D3). ICG-FI was performed during baseline (T0), occlusion (T1), reperfusion (T2) and after an observation period of 60 minutes (T4). Results All experiments could be finished successfully. ICG-FI was assessed using assessment of background-subtracted peak fluorescence intensity (BSFI), slope of fluorescence intensity (SFI), and a baseline adjusted ratio of both parameters. ICG-FI confirmed loss of perfusion in D1, decreased perfusion in D2, and increased perfusion in D3. After reperfusion ICG-FI increased in group 2 due to a severe tissue damage resulting in a capillary leakage. In group I ICG-FI was equal to baseline values indicating the totally reversible loss of perfusion. Conclusion Using ICG-FI to estimate intestine perfusion after different durations of ischemia is viable using a porcine model of mesenteric ischemia. Even small differences in perfusion can be reliably determined by ICG-FI. Thus, ICG-FI is an encouraging method to evaluate intestine perfusion intraoperatively.

Facile Process for Preparation of PS/PtOEP Composite Microspheres and their Oxygen Sensing Properties

2014 ◽  
Vol 1004-1005 ◽  
pp. 307-310
Author(s):  
Song Li ◽  
Ke Zhang ◽  
Su E Hao
Keyword(s):  
Fluorescence Intensity ◽  
Dispersion Polymerization ◽  
Monomer Concentration ◽  
Excitation Wavelength ◽  
Ultrapure Water ◽  
Fluorescent Substance ◽  
Composite Microspheres ◽  
Dispersing Agent ◽  
Intensity Changes ◽  
Content Of Oxygen

In this study, the monodispersed PS/PtOEP microspheres were successfully synthesized by dispersion polymerization, in which anhydrous ethanol and ultrapure water as dispersing agent, AIBN as an initiator, styrene as monomer, PtOEP as the fluorescent substance. Polystyrene microspheres with the size of the initiator, dispersant, the amount of monomer and so on, and has nothing to do with PtOEP. We can be obviously seen under scanning electron microscope. When in the same content of PtOEP, the particle size of composite microspheres size increases with increasing monomer concentration. When the excitation wavelength was 362nm, the PtOEP peak measured emission spectrum at 720nm. With the increasing size of composite microspheres, the peak fluorescence intensity changes. From the S-V diagram, we observed that the concentration of luminous intensity of composite microspheres monotonously dependent on oxygen, there is a linear correlation between PtOEP in 720nm fluorescence intensity and the concentration of oxygen. This method can be utilized to reliably monitor the content of oxygen in the system.

Fluorescence-intensity changes in organic dyes impregnated in porous glass on exposure to NO2

1999 ◽  
Vol 59 (1) ◽  
pp. 16-20 ◽  
Author(s):  
Takashi Ohyama ◽  
Yasuko Yamada Maruo ◽  
Tohru Tanaka ◽  
Takayoshi Hayashi
Keyword(s):  
Fluorescence Intensity ◽  
Porous Glass ◽  
Organic Dyes ◽  
Intensity Changes

Influence of vessel stenosis on indocyanine green fluorescence intensity assessed by near-infrared fluorescence angiography

Surgery Today ◽  
2016 ◽  
Vol 47 (7) ◽  
pp. 877-882 ◽  
Author(s):  
Masaki Yamamoto ◽  
Hideaki Nishimori ◽  
Takashi Fukutomi ◽  
Takemi Handa ◽  
Kazuki Kihara ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Fluorescence Intensity ◽  
Near Infrared ◽  
Fluorescence Angiography ◽  
Green Fluorescence ◽  
Near Infrared Fluorescence ◽  
Indocyanine Green Fluorescence ◽  
Vessel Stenosis

Quantitative Comparison of the Intraoperative Utility of Indocyanine Green and Fluorescein Videoangiographies in Cerebrovascular Surgery

2017 ◽  
Vol 13 (3) ◽  
pp. 361-366 ◽  
Author(s):  
Fumihiro Matano ◽  
Takayuki Mizunari ◽  
Yasuo Murai ◽  
Asami Kubota ◽  
Yu Fujiki ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Indocyanine Green ◽  
Fluorescence Intensity ◽  
Artery Bypass ◽  
Superficial Temporal Artery ◽  
Arterial Stenosis ◽  
Anterior Communicating Artery ◽  
Parent Artery ◽  
Perforating Artery ◽  
Cerebrovascular Surgery

Abstract BACKGROUND: A quantitative analysis comparing indocyanine green videoangiography (ICG-VAG) and fluorescein videoangiography (FL-VAG) in cerebrovascular surgery has not been reported so far. OBJECTIVE: To clear the differences of characteristics of ICG-VAG and FL-VAG by quantitative assessment. METHODS: We prospectively analyzed results from 23 patients (3 males; mean age at surgery: 60.9 years, range: 14-75 years) at our hospital from August 2014 to July 2015. Eighteen patients had cerebral aneurysms for clipping, and 5 had intracranial arterial stenosis for superficial temporal artery (STA)–middle cerebral artery bypass. We imported data from the operative image data, converted by Audio Video Interleave to Aquacosmos as picture fluorescence intensity-analyzing software. Regions of interest were set at the parent artery, dome of aneurysms, and perforating artery in cases of clipping of aneurysms, and setting at 3 points in STA, in case of bypass. The transition of fluorescence intensity at each region of interest was calculated and plotted using Aquacosmos. RESULTS: Thick-walled artery, such as parent artery (P = .0017) and STA (P = .0182), was more significantly visualized by ICG-VAG than FL-VAG, whereas the perforating artery, especially in deep surgical fields, such as anterior communicating artery, internal carotid artery, and basilar artery, was better visualized by FL-VAG than ICG-VAG (P &lt; .0001). CONCLUSION: In this quantitative analysis of fluorescence study, ICG-VAG showed greater efficacy than FL-VAG in visualizing relatively thick arteries, such as parent artery and STA. However, FL-VAG has greater efficacy than ICG-VAG in visualizing perforating artery, especially in deep surgical fields with characteristic vessel walls.

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