scholarly journals Evaluation of the cytotoxic and genotoxic effect of garlic (Allium sativum L.) root cells after exposure to contaminant solution

2020 ◽  
Vol 13 (3) ◽  
pp. 6
Author(s):  
L. P. N. Ramos ◽  
L. S. D. Nascimento ◽  
J. S. Lima ◽  
C. B. M. Farias ◽  
V. S. Mello ◽  
...  
Keyword(s):  
Indirect Method ◽  
Allium Sativum ◽  
Chemical Nature ◽  
Direct Method ◽  
Root Cells ◽  
Mato Grosso ◽  
Index Analysis ◽  
Mutagenic Effects ◽  
The University ◽  
A Direct Method

Water contamination is much more common than imagined, because it is practically impossible today to have an ecosystem that has not suffered directly or indirectly from humans. Studies with the aim of genotoxicity and mutagenesis use bioindicators as the basis of tests, the most used are from the Allium ssp family. When we mention cytogenetic damage, the intensity depends mainly on the degree of exposure, quantity, chemical nature and of the possible combinations between the contaminants, also influenced by the characteristics and conditions of the environment in which the organisms considered bioindicators are submitted. The present work has the objective of analyzing the cytotoxic, genotoxic and mutagenic effects induced by contaminating material that cause chromosomal alterations by means of mitotic index analysis using as bioindicator Allium sativum (garlic). The experiment was carried out at the Laboratory of Cytogenetics and Culture of Vegetable Tissues at the University of the State of Mato Grosso (UNEMAT) at the Alta Floresta Campus - MT. The soil was collected and stored separately using 200 grams of each sample with three treatments: surface, 20 cm and 40 cm deep, making a tea with 200 ml of distilled water and then measuring the pH. Using a direct method where the bioindicator is submitted directly to the tea solution, awaiting the emission of roots, and the indirect method, where the bioindicator is submitted first, the stimulation of root formation for 72 hours and then is submitted to contaminating solution. The plates readings to observe the interior of the cell using crushing techniques were performed in 24, 48 and 72 hours of experiment (indirect method) and 24 hours (direct method, also measuring the roots). With the results, we can state that the material used as pollutant (soil sample) has a cytotoxic and genotoxic potential in the bioindicator material, presenting high potential in the cell cycle induction, provoking various chromosomal aberrations.

Flow Measurement to a Kaplan Turbine Using a Direct Method and an Indirect One

2016 ◽  
Vol 823 ◽  
pp. 361-366
Author(s):  
Ana Maria Budai ◽  
Adrian Cuzmos ◽  
Cristian Fanica ◽  
Damaschin Pepa ◽  
Cosmin Ursoniu ◽  
...  
Keyword(s):  
Direct Measurement ◽  
Flow Rate ◽  
Flow Measurement ◽  
Indirect Method ◽  
Direct Method ◽  
The Other ◽  
Hydropower Plant ◽  
Kaplan Turbine ◽  
A Direct Method

The paper present two different methods for measuring flow in hydropower, one direct and one indirect method. The study consists in comparing the results obtained by measurements made by both methods in a Romanian hydropower plant. The indirect method used is the Winter-Kennedy method. The other method involves direct measurement of the flow rate of water using a specialized outfit in this.

Isoperimetric Problems in the Calculus of Variations

1952 ◽  
Vol 4 ◽  
pp. 257-280 ◽  
Author(s):  
William Karush
Keyword(s):  
Calculus Of Variations ◽  
Classical Theory ◽  
Indirect Method ◽  
Direct Method ◽  
Isoperimetric Problems ◽  
End Points ◽  
Sufficiency Theorems ◽  
Theory Of Fields ◽  
A Direct Method ◽  
Problem Of Bolza

We are concerned with establishing sufficiency theorems for minima of simple integrals of the parametric type in a class of curves with variable end points and satisfying isoperimetric side conditions. The results which are obtained involve no explicit assumptions of normality. Such results can be derived by transforming our problem to a problem of Bolza and using the latest developments in the theory of that problem. More recently [6] an indirect method of proof has been published. Our object is to present a direct method of proof without transformation of the problem which is based upon a generalization of the classical theory of fields.

scholarly journals Estimating emission from gas power plant in palembang, indonesia using direct and indirect methods

2018 ◽  
Vol 67 ◽  
pp. 02037
Author(s):  
Gabriel Andari Kristanto ◽  
Alditho Farrasi Anantoputra
Keyword(s):  
Power Plant ◽  
Emission Factor ◽  
Indirect Method ◽  
Emission Inventory ◽  
Direct Method ◽  
Total Particulate Matter ◽  
Indirect Methods ◽  
Gas Power Plant ◽  
A Direct Method ◽  
Direct And Indirect Methods

Nowadays, almost every daily activity is supported by the availability of energy, especially electricity. As the Borang 60 MW Gas Power Plant in Palembang, Indonesia emits air pollutants such as SO2, NOx, CO, and total particulate matter (total PM), an emission inventory needs to be developed. Various methods can be used in the inventory of emissions; among others are a direct method based on direct measurement and an indirect method which involves the calculation of total emissions by emission factor. This study aims to estimate the emissions from the gas power plant in Palembang using direct and indirect methods and analyze the most suitable emission factor for Indonesian conditions. The comparison between emissions measured by direct and indirect methods found that the indirect method using the emission factor of AP-42 gives more accurate results for SO2 and NOx, whereas the total emission of CO is more accurately calculated based on the indirect method using the emission factor developed by Kurokawa et al. (2013); the median value of total PM is more accurately calculated based on the indirect method using the emission factor developed by the IPCC.

scholarly journals Wittgenstein and Surrealism

2012 ◽  
Vol 13 (1) ◽  
pp. 74-84
Author(s):  
Chrysoula Gitsoulis ◽  
Keyword(s):  
Indirect Method ◽  
Direct Method ◽  
Language Games ◽  
Linguistic Practice ◽  
A Direct Method

There are two aspects to Wittgenstein’s method of deconstructing pseudo-philosophical problems that need to be distinguished: (1) describing actual linguistic practice, and (2) constructing hypothetical ‘language-games’. Both methods were, for Wittgenstein, indispensable means of clarifying the ‘grammar’ of expressions of our language – i.e., the appropriate contexts for using those expressions – and thereby dissolving pseudo-philosophical problems. Though (2) is often conflated with (1), it is important to recognize that it differs from it in imprtant respects. (1) can be seen as functioning as a direct method of ‘proof’ (i.e., attempt to convince the reader of some thesis), and (2) as an indirect method of ‘proof’ – proof by reduction ad absurdum. This essay will be devoted to clarifying (2) by forging an analogy with surrealism in art.

scholarly journals Zero Spatial Frequency Limit: Method to Characterize Photopolymers as Optical Recording Material

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Sergi Gallego ◽  
Andrés Márquez ◽  
Manuel Ortuño ◽  
Cristian Neipp ◽  
Inmaculada Pascual ◽  
...  
Keyword(s):  
Spatial Frequency ◽  
Data Storage ◽  
Direct Method ◽  
Optical Recording ◽  
Optical Processing ◽  
Optical Elements ◽  
Recording Material ◽  
The University ◽  
A Direct Method ◽  
Processing Group

Photopolymers are useful for different holographic applications such as holographic data storage or diffractive optical elements. However, due to the presence of two different phenomena, polymer formation and monomer diffusion, it is difficult to characterize each parameter independently. We propose a direct method based on zero spatial frequency recording, to eliminate the diffusion influence, and on interferometric techniques, both in transmission and in reflection, to obtain quantitative values of shrinkage, polymerization rate, polymer refractive index and relation between intensity and polymerization, and so forth, This method has been implemented in the Holography and Optical Processing Group from the University of Alicante to characterize different photopolymers. In this paper, we present a compilation of the results obtained with this method for different photopolymers and we compare their characteristics.

Determination of the Speed of an Unmanned Rescue Vehicle

2015 ◽  
Vol 220-221 ◽  
pp. 940-945 ◽  
Author(s):  
Andrzej Zbrowski ◽  
Wojciech Jóźwik
Keyword(s):  
Rotational Speed ◽  
Indirect Method ◽  
Direct Method ◽  
Indirect Measurement ◽  
Measurement Range ◽  
Test System ◽  
Correct Operation ◽  
Linear Speed ◽  
A Direct Method

The article presents the process of determining the speed of an unmanned rescue vehicle.The conducted investigations involved a measurement system developed for testing dynamic lorries. The application of a test system for low-speed all-terrain vehicles was possible due to a wide measurement range of sensors. Speed was tested employing two following methods:an indirect method consisting of the measurement of the instantaneous rotational speed of the wheel;a direct method consisting of the measurement of speed against the ground, taken with an optical speed sensor.The authors also describe the sources of interferences characteristic of both methods that include:the wheelspin affecting the correctness of speed determination in the indirect measurement;the unevenness of the surface (ground) hindering the correct operation of the optical speed sensor.The paper also presents the characteristics of the rotational speed of the wheels obtained in road and off-road conditions. Based on the obtained signals, linear speed was determined. Additionally, the article discusses methods for eliminating measurement disturbances.

Biomass active fraction evaluated by a direct method and respirometric techniques

2002 ◽  
Vol 46 (1-2) ◽  
pp. 371-379 ◽  
Author(s):  
G. Andreottola ◽  
P. Foladori ◽  
A. Gelmini ◽  
G. Ziglio
Keyword(s):  
Flow Cytometry ◽  
Indirect Method ◽  
Wastewater Treatment Plants ◽  
Direct Method ◽  
Bacterial Biomass ◽  
Active Fraction ◽  
Active Biomass ◽  
Viable Bacteria ◽  
Viable Biomass ◽  
A Direct Method

The knowledge of the active biomass amount and its characterisation is of primary importance for the management and for the design of wastewater treatment plants on the basis of the recently developed models. OUR curves obtained in aerobic exponential growth tests are proposed by several authors as an indirect method to estimate the active fraction. The aim of this work is the application of a direct method to measure the viable biomass based on flow cytometry techniques and the comparison with the active fraction obtained from respirometric tests. To assess the viable fraction of a biomass expressed in terms of COD concentration it is necessary to estimate the biovolume of viable bacteria and to know the specific carbon content per cell. For the investigated activated sludge samples, the bacterial biomass measured by FCM was 588 mgCOD L−1 on average in a two-months period. This value of active fraction corresponds to 14% of particulate COD. Active biomass values measured with the respirometric approach were consistent but generally higher than those obtained by FCM.

scholarly journals Release of <sup>222</sup>Rn from some soils

1997 ◽  
Vol 15 (1) ◽  
pp. 124-133 ◽  
Author(s):  
C. Dueñas ◽  
M. C. Fernández ◽  
J. Carretero ◽  
E. Liger ◽  
M. Pérez
Keyword(s):  
Concentration Profile ◽  
Thermal Gradient ◽  
Indirect Method ◽  
Direct Method ◽  
Soil Air ◽  
Static Chamber ◽  
Flux Measurements ◽  
A Direct Method ◽  
Static Chamber Technique ◽  
Chamber Technique

Abstract. Measurements have been made of 222Rn release from diverse soils in the region surrounding Málaga, Spain. These flux measurements were carried out by two methods. A direct method using a static chamber technique and another indirect method obtained from concentration profile measurements of 222Rn in the soil air. The effects of meteorological variables and other parameters on 222Rn flux were studied. The factors that most affected the instantaneous value of 222Rn release were humidity and soil thermal gradient. The directly measured 222Rn fluxes at investigated sites are higher than 222Rn fluxes derived by the indirect method.

scholarly journals Analisa Eisiensi Economizer Terhadap Boiler (Gas Dan Solar) Di PT. Spindo III, Tbk

2018 ◽  
Vol 2 (2) ◽  
pp. 99
Author(s):  
Finus Ainun ◽  
Jamaaluddin Jamaaluddin
Keyword(s):  
Indirect Method ◽  
Direct Method ◽  
Pipe Production ◽  
Feed Water ◽  
Average Yield ◽  
Indirect Methods ◽  
A Direct Method ◽  
Galvanize Pipe ◽  
Boiler Feed Water

The role of boiler machines at PT. SPINDO in the galvanize pipe production process is very important, where the steam produced by the fire tube boiler machine is directly distributed into the blow system for the cleaning of the remaining galvanic in the pipe and heating the water for pipe washing. To determine the efficiency of the boiler, a direct method is used to analyze the efficiency of boiler machines and indirect methods as a way of calculating the amount of efficiency if an economizer is installed. The efficiency of diesel-fueled boilers using the direct method yields an average yield of 40.7% (forty point seven percent) and 45.84% (forty five point eighty four percent) for gas-fired boilers. However, by using an indirect method, efficiency if installed economizer is obtained at 85.55% (eighty five point fifty five percent) for diesel-fueled boilers with a capacity of 5 tons and 81.76% (eighty one point seventy six percent) of the efficiency of a gas-fired boiler with a capacity of 2 (two) tons. Efficiency improvements can also be done in many ways, including maintenance of a good and scheduled boiler machine for good boiler feed water treatment.  

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