scholarly journals THE INFLUENCE OF DRUG TREATMENT ON CARDIO-VASCULAR SYSTEM OF THE RATS WITH CONGENITAL HYPOTHYROIDISM

2019 ◽  
Vol 72 (7) ◽  
pp. 1300-1303
Author(s):  
Svetlana Chuhray ◽  
Viktoria Lavrynenko ◽  
Rostyslav Kaminsky ◽  
Olena Ustymenko ◽  
Iryna Dzevulska ◽  
...  
Keyword(s):  
Congenital Hypothyroidism ◽  
Structural Changes ◽  
Vascular System ◽  
Ventricular Myocardium ◽  
Left Ventricular ◽  
The Body ◽  
Left Ventricular Myocardium ◽  
Substitution Therapy ◽  
Blood Capillaries ◽  
Pharmacological Correction

Introduction: In the treatment of hypothyroidism substitution therapy with L-thyroxine is used, it is also advisable to use the metabolites with membrane-stabilizing properties that normalize the metabolism in the body, for example, calcitonin, which significantly reduces the depth of the dystrophic phenomena in the myocardium. The aim was to study the patterns of structural changes in the left ventricular myocardial capillaries of rats with congenital hypothyroidism in combinative drug therapy with L-thyroxine and calcitonin. Materials and methods: 30 white Wistar line rats were used as experimental animals: 10 with treatment and 10 without as well as control – 10 intact Wistar line rats of the same age. Mercazolil was used to inhibit thyroid gland in order to model congenital hypothyroidism. After birth, the rats received L-thyroxine at a dose of 10 μg / kg per os daily, calcitonin at a dose of 1.0 MU / kg per day intramuscularly, then with mother’s milk, later by themselves for 100 days. The arterial pressure was measured in all experimental groups during extraction from the experiment by plethysmograph, their left ventricular myocardium was examined under electron microscope and micropinocytotic vesicles in their cells were studied morphometrically. Results: In rats with congenital hypothyroidism, for which L-thyroxin drug in combination with calcitonin was used as a substitution therapy, after pharmacological correction, in general there is no pronounced heteromorphism of the ultrastructure of the left ventricular myocardial blood capillaries, which was characteristic for animals without pharmacological correction. The analysis showed normalization of the content of free thyroxine in blood plasma and blood pressure of rats with congenital hypothyroidism after complex substitution therapy. Conclusions: In rats with congenital hypothyroidism, which received L-thyroxine and calcitonin at birth, the myocardium capillaries generally remain intact and have morphological and functional characteristics similar to intact animals, which is the theoretical basis for the need for calcitonin to be used in substitution therapy in hypothyroidism.

scholarly journals MORPHOFUNCTIONAL STATUS OF CARDIO-VASCULAR SYSTEM OF RATS WITH CONGENITAL HYPOTHYREOSIS

2019 ◽  
Vol 72 (2) ◽  
pp. 229-233 ◽  
Author(s):  
Svitlana M. Chuhray ◽  
Viktoria E. Lavrynenko ◽  
Rostyslav F. Kaminsky ◽  
Iryna V. Dzevulska ◽  
Olexandr V. Malikov ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Congenital Hypothyroidism ◽  
Vascular System ◽  
Left Ventricular ◽  
Transport Processes ◽  
Morphometric Study ◽  
Left Ventricular Myocardium ◽  
Moderate Amount ◽  
Blood Capillaries ◽  
Sexually Mature

Introduction: There is an increasing number of cases of congenital hypothyroidism. One of the most common complications of hypothyroidism is damage to the cardiovascular system, which in 30-50% of patients leads to the development of arterial hypertension. The aim: studying the features of the ultrastructure of myocardial capillaries in mature rats with hypothyroidism. Materials and methods: Experiments were conducted on 40Wistar line rats with congenital hypothyroidism: juvenile young (45-day) and sexually mature (100-day) rats, as well as intact animals of the corresponding age. While extracted from the experiment rats of all experimental groups had their arterial pressure measured using a plethysmograph while the development of hypothyroidism was controlled by the immune-enzymatic method. Electron microscopic examination of the left ventricular myocardium and morphometric study of volumetric and quantitative densities, cross-section area, and form factor of micropinocytotic vesicles were conducted. Results: In the sexually mature rats with congenital hypothyroidism the quantitative density of the capillaries in the myocardium decreases. Activation of transcytosis is accompanied by significant violations of vesiculation. Some of the endothelial cells of experimental animals contain a moderate amount of transport vesicles, while others are overfilled with these structures and desquamate into the lumen. In older rats with congenital hypothyroidism there is a further dilution of capillaries, the development of hypoxic state in them, mucinous edema of interstitial space, decrease of biosynthetic and transport processes activity. Conclusions: In young (45-day) rats with congenital hypothyroidism the direction and expressiveness of compensatory processes is to enhance the transcytosis processes. Dystrophicdestructive changes are manifested by apoptosis in some endothelial cells, decrease in the number of biosynthetic organelles, lysis and edema of their cytoplasm. In sexually mature (100-day) rats with congenital hypothyroidism destructively-dystrophic processes in the blood capillaries of the myocardium are approximately balanced with compensatory-adaptive.

scholarly journals INFLUENCE OF COMBINED PHARMACOTHERAPY ON CARDIO-VASCULAR SYSTEM OF ARTERIAL HYPERTENSION (IN EXPERIMENT)

2020 ◽  
Vol 73 (8) ◽  
pp. 1712-1716
Author(s):  
Svetlana N. Chuhray ◽  
Viktoria E. Lavrynenko ◽  
Ruzhena M. Matkivska ◽  
Tetiana V. Lachtadyr ◽  
Valentina M. Hamalii ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Arterial Pressure ◽  
Arterial Hypertension ◽  
Structural Changes ◽  
Vascular System ◽  
Ventricular Myocardium ◽  
Left Ventricular ◽  
Morphometric Study ◽  
Left Ventricular Myocardium ◽  
Electron Microscopic

DOI: 10.36740/ BOGOMOLETS NATIONAL MEDICAL UNIVERSITY, KYIV, UKRAINE, TARAS SHEVCHENKO NATIONAL UNIVERSITY OF KYIV, KYIV, UKRAINE 3KYIV CITY CLINICAL EMERGENCY HOSPITAL, KYIV, UKRAINE ABSTRACT The aim: Study of the patterns of structural changes in the left ventricular myocardial capillaries of rats with arterial hypertension with combined pharmacotherapy with Bisoprolol and Thiotriazolinum. Materials and methods: Experiments were conducted on 30 line rats with congenital stress-induced arterial hypertension: 10 animals without treatment and 10 animals with treatment. Pharmacological correction of spontaneous arterial hypertension was performed with 20 mg / kg of Bisoprolol and 50 mg / kg of Thiotriazolinum per os once a day. Pharmacotherapy began at 5 months of age, that is, at a time when compensated heart failure was formed in rats with arterial hypertension. Animals were withdrawn from the experiment 100 days after the start of the correction. Control was provided by intact animals (10 rats) of the corresponding age. While extracted from the experiment rats of all experimental groups had their arterial pressure measured using a plethysmograph, electron microscopic examination of the left ventricular myocardium and morphometric study of volumetric and quantitative densities, cross-section area and form factor of micropinocytotic vesicles were conducted. Results: In rats with arterial hypertension after application of Bisoprolol and Thiotriazolinum, arterial pressure significantly decreases in experimental rats compared to animals without correction. The number of capillaries in the myocardium after pharmacotherapy increases up to control values, which shows their reparation. In most endothelial cells, organelles retain their integrity and presence that are characteristic of intact rats. The well-expressed processes of transcytosis are shown by the statistical similarity of the quantitative density and the size of the micropinocytotic vesicles in the endothelial cells of the myocardium capillaries of compared experimental animals. Conclusions: In rats with arterial hypertension, the combination of Bisoprolol and Thiotriazolinum prevents the decrease in the number of capillaries in the myocardium of the left ventricle, promotes the preservation of the ultrastructure of their endothelial cells and maintains the processes of transedothelial transfer of substances at the level of intact animals.

scholarly journals Remodeling of the heart and main vessels in patients with marfanoid habitus

2020 ◽  
Vol 10 (5) ◽  
pp. 27-34
Author(s):  
Eugene V. Timofeev ◽  
Eduard G. Malev ◽  
Nina N. Parfenova ◽  
Eduard V. Zemtsovsky
Keyword(s):  
Marfan Syndrome ◽  
Aortic Root ◽  
Structural Changes ◽  
Systolic Function ◽  
Interventricular Septum ◽  
Age Groups ◽  
Ventricular Myocardium ◽  
Left Ventricular ◽  
Control Group ◽  
Left Ventricular Myocardium

For many hereditary connective tissue disorders (HCTD), especially Marfan syndrome, remodeling of the heart and main vessels is described, which is manifested by a decrease in the systolic function of the left ventricle and expansion of the thoracic aorta. Evaluation of morphometric characteristics of the heart and main vessels in patients with other HCTD, in particular marfanoid habitus (MH) has not been previously carried out. Materials and methods. Weexamined 90 young men and 74 young women between the ages of 18 to 25 years, 111 patients older age groups with stable over coronary heart disease (mean age 64.66.2 years) and 9 patients with verified Marfan syndrome (mean age 27.99.3years). All survey phenotypic and performed anthropometric survey identifying bone signs of dysembryogenesis as well as Echocardiography study on standard protocol. The results.Patients with MH as compared with control group revealed a relatively larger diameter of aortic root (30.44.7 vs 28.03.6 mm,p= 0.03) and the ascending aorta (26.64.9 vs 24.63.2 mm,p= 0.05). Also young with MH turned out to be significantly thicker myocardium of left ventricular posterior wall (8.30.8 vs 7.71.1 mm,p= 0.02) and interventricular septum (8.81.2vs 8.21.1mm,p= 0.04). When performing correlation analysis identified reliable positive correlation between such highly specialized bone signs as high palate (r= 0.31), infundibular deformation of the chest (r= 0.43), arachnodactyly (r= 0.45) andZ-test (p 0.05 for all). Expansion of the aorta (Z-criterion 2.0) have found 24% of older patients with MH. Conclusion.Inpatients with MH revealed significant structural changes of heart and main vessels which are progredient character thickening of the left ventricular myocardium and expansion of the aortic root.

scholarly journals Contributions of the 12 Segments of Left Ventricular Myocardium to the Body Surface Potentials

2007 ◽  
pp. 300-309
Author(s):  
Juho Väisänen ◽  
Jesús Requena-Carrión ◽  
Felipe Alonso-Atienza ◽  
Jari Hyttinen ◽  
José Luis Rojo-Álvarez ◽  
...  
Keyword(s):  
Body Surface ◽  
Ventricular Myocardium ◽  
Left Ventricular ◽  
The Body ◽  
Left Ventricular Myocardium ◽  
Surface Potentials

scholarly journals Morphofunctional status of cardio-vascular system of rats with arterial hypertension

2020 ◽  
Vol 73 (2) ◽  
pp. 355-359 ◽  
Author(s):  
Svetlana M. Chuhray ◽  
Viktoria E. Lavrynenko ◽  
Rostyslav F. Kaminsky ◽  
Iryna V. Dzevulska ◽  
Oleksandr V. Malikov ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Arterial Pressure ◽  
Arterial Hypertension ◽  
Vascular System ◽  
Left Ventricular ◽  
Morphometric Study ◽  
Left Ventricular Myocardium ◽  
Blood Capillaries ◽  
Sexually Mature

The aim: Was to clarify the general patterns of structural changes in the left ventricular myocardial capillaries in rats with spontaneous arterial hypertension. Materials and methods: Experiments were conducted on 50 ISIAH (inherited stress-induced arterial hypertension) line rats with arterial hypertension: juvenile young (45-day) and sexually mature (100-day) rats, as well as intact animals of the corresponding age. While extracted from the experiment rats of all experimental groups had their arterial pressure measured using a plethysmograph. Electron microscopic examination of the left ventricular myocardium and morphometric study of volumetric and quantitative densities, cross-section area, and form factor of micropinocytotic vesicles were conducted. Results: In sexually mature rats with arterial hypertension, a high level of pressure is maintained. In 45-day-old rats with arterial hypertension in endothelial cells of myocardial blood capillaries there is a hyperactivation of biosynthetic processes (euchromatic nucleus, large-sized mitochondria, ER canals, Golgi complex), which may be a manifestation of reactive processes in response to a non-stable increase in arterial pressure. In the 100-day rats with arterial hypertension, the mosaic of the ultrastructure of the myocardium blood vessels is preserved, but destructively-dystrophic changes become more expressive and involve not only the organelles but also the integrity of the endothelial cell itself. Destructively-dystrophic processes in rat capillaries are accompanied by compensatory and adaptive ones. This is manifested by activation of the transport of substances, both transendothelial and paracellular, and quantitative density of micropinocytotic vesicles increases statistically significantly. Conclusions: In myocardial capillaries of young (45-day) arterial hypertension rats, compensatory and adaptive changes are manifested by activation of biosynthetic processes in endothelial cells following a slight increase in micropinocytotic vesicles quantitative density and signs of destructive-dystrophic processes (minor edema and lysis of endothelial cell cytoplasm). In sexually mature (100-day) arterial hypertension rats in the blood capillaries of the myocardium, the destructive-degenerative changes increase is accompanied by preservation of signs of compensatory processes. Reducing the number of capillaries is offset by an increase in the number of micropinocytotic vesicles.

Hypertrabecular aspect of left ventricular myocardium: A possible complication of congenital hypothyroidism in a preterm infant

2010 ◽  
Vol 23 (7) ◽  
pp. 732-735
Author(s):  
Francesca Paola Fusco ◽  
Paola Sindico ◽  
Maria Pia De Carolis ◽  
Simonetta Costa ◽  
Francesco Cota ◽  
...  
Keyword(s):  
Preterm Infant ◽  
Congenital Hypothyroidism ◽  
Ventricular Myocardium ◽  
Left Ventricular ◽  
Left Ventricular Myocardium

Localization of preproenkephalin mRNA in rat heart: selective gene expression in left ventricular myocardium

1998 ◽  
Vol 275 (2) ◽  
pp. H378-H384 ◽  
Author(s):  
Joachim Weil ◽  
Thomas Eschenhagen ◽  
Gerrit Fleige ◽  
Clemens Mittmann ◽  
Ellen Orthey ◽  
...  
Keyword(s):  
Left Ventricle ◽  
Opiate Receptors ◽  
Ventricular Myocardium ◽  
Northern Blotting ◽  
Postnatal Period ◽  
Left Ventricular ◽  
The Body ◽  
Left Ventricular Myocardium ◽  
Mrna Levels ◽  
The Right

The enkephalins are derived from a common precursor protein known as preproenkephalin (ppENK). Enkephalins appear to be one of the endogenous ligands for the opiate receptors. In the rat the ventricular myocardium contains more ppENK mRNA than any other tissue. To gain further insight into the role of cardiac enkephalins, the regional and developmental distribution of ppENK mRNA was studied by Northern blotting and in situ hybridization. In the early postnatal period, ppENK mRNA is low in atrial and ventricular myocardium. With maturation, ppENK expression increases threefold in left and right ventricular tissue, but not in the atria or cardiac conductive system. Interestingly, ppENK mRNA levels are four times higher in the left than in the right chamber. Thus, to our knowledge, ppENK is the only gene exhibiting marked differences in expression between the adult right and left ventricle. Given the left-side preference of ppENK expression, the possibility is raised that the left ventricle is an endocrine organ that supplies the body with enkephalins.

scholarly journals Effects of Telmisartan on Myocardial Protein Profiles in Hypertensive Rats

2021 ◽  
Vol 34 (3) ◽  
pp. 299-299
Author(s):  
Yu Feng ◽  
Man-li Zhou ◽  
Jian-zhang Wang ◽  
Jia-qi Zhang ◽  
Shu-le Qian ◽  
...  
Keyword(s):  
Heat Shock ◽  
Ventricular Myocardium ◽  
Left Ventricular ◽  
Left Ventricular Myocardium ◽  
Sterile Water ◽  
Protein Profiles ◽  
Related Protein ◽  
Hypertensive Rats ◽  
Treatment Groups ◽  
Proteasome 26S

Abstract Background To investigate the effects of telmisartan on the protein profiles of the left ventricular myocardium in spontaneously hypertensive rats (SHR). Methods Sixteen SHR were randomly divided into control and telmisartan treatment groups. Rats were treated with sterile water (10 ml/kg) or telmisartan (4.33 mg/kg) by gavage for 12 weeks. Wistar-Kyoto (WKY) rats treated with sterile water (10 ml/kg) as controls. At the end of 12 weeks of control or telmisartan treatment, rats were sacrificed, and hearts were collected for protein preparations, isotope labeling, and mass spectrometric analysis. Results In total, there were 23 differentially expressed proteins in the left ventricular myocardium between control and telmisartan treatment groups in SHR. Compared with the telmisartan group, the upregulated proteins in the SHR were dual-specificity mitogen-activated protein kinase kinase 3-like, transgelin, and haptoglobin subtype 2. The downregulated proteins in the SHR were as follows: von Willebrand factor (fragment), kininogen 1, small ribonucleoprotein-related protein, fibrinogen beta chain, protein mass 3 (fragment), proteasome 26s, heat shock protein 27-related protein 1, tenascin X, fibronectin subtype 2, transferrin receptor protein, platelets 1, cathepsin L1, complement factor B, isoform CRA_b, fibrinogen isomer, immunoglobulin heavy chain (γ polypeptide), and α 1 antiprotease. Conclusions Telmisartan differentially regulates myocardial protein expression in hypertensive rats including heat shock protein 27, fibrinogen, fibronectin, proteasome 26s and transgelin, as well as proteins in biochemical, metabolic, and signal transduction pathways. These changes in protein expression may contribute to the antihypertrophic effects of telmisartan in hypertension.

Sign in / Sign up

Export Citation Format

Share Document