scholarly journals MORPHOFUNCTIONAL STATUS OF CARDIO-VASCULAR SYSTEM OF RATS WITH CONGENITAL HYPOTHYREOSIS

2019 ◽  
Vol 72 (2) ◽  
pp. 229-233 ◽  
Author(s):  
Svitlana M. Chuhray ◽  
Viktoria E. Lavrynenko ◽  
Rostyslav F. Kaminsky ◽  
Iryna V. Dzevulska ◽  
Olexandr V. Malikov ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Congenital Hypothyroidism ◽  
Vascular System ◽  
Left Ventricular ◽  
Transport Processes ◽  
Morphometric Study ◽  
Left Ventricular Myocardium ◽  
Moderate Amount ◽  
Blood Capillaries ◽  
Sexually Mature

Introduction: There is an increasing number of cases of congenital hypothyroidism. One of the most common complications of hypothyroidism is damage to the cardiovascular system, which in 30-50% of patients leads to the development of arterial hypertension. The aim: studying the features of the ultrastructure of myocardial capillaries in mature rats with hypothyroidism. Materials and methods: Experiments were conducted on 40Wistar line rats with congenital hypothyroidism: juvenile young (45-day) and sexually mature (100-day) rats, as well as intact animals of the corresponding age. While extracted from the experiment rats of all experimental groups had their arterial pressure measured using a plethysmograph while the development of hypothyroidism was controlled by the immune-enzymatic method. Electron microscopic examination of the left ventricular myocardium and morphometric study of volumetric and quantitative densities, cross-section area, and form factor of micropinocytotic vesicles were conducted. Results: In the sexually mature rats with congenital hypothyroidism the quantitative density of the capillaries in the myocardium decreases. Activation of transcytosis is accompanied by significant violations of vesiculation. Some of the endothelial cells of experimental animals contain a moderate amount of transport vesicles, while others are overfilled with these structures and desquamate into the lumen. In older rats with congenital hypothyroidism there is a further dilution of capillaries, the development of hypoxic state in them, mucinous edema of interstitial space, decrease of biosynthetic and transport processes activity. Conclusions: In young (45-day) rats with congenital hypothyroidism the direction and expressiveness of compensatory processes is to enhance the transcytosis processes. Dystrophicdestructive changes are manifested by apoptosis in some endothelial cells, decrease in the number of biosynthetic organelles, lysis and edema of their cytoplasm. In sexually mature (100-day) rats with congenital hypothyroidism destructively-dystrophic processes in the blood capillaries of the myocardium are approximately balanced with compensatory-adaptive.

scholarly journals Morphofunctional status of cardio-vascular system of rats with arterial hypertension

2020 ◽  
Vol 73 (2) ◽  
pp. 355-359 ◽  
Author(s):  
Svetlana M. Chuhray ◽  
Viktoria E. Lavrynenko ◽  
Rostyslav F. Kaminsky ◽  
Iryna V. Dzevulska ◽  
Oleksandr V. Malikov ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Arterial Pressure ◽  
Arterial Hypertension ◽  
Vascular System ◽  
Left Ventricular ◽  
Morphometric Study ◽  
Left Ventricular Myocardium ◽  
Blood Capillaries ◽  
Sexually Mature

The aim: Was to clarify the general patterns of structural changes in the left ventricular myocardial capillaries in rats with spontaneous arterial hypertension. Materials and methods: Experiments were conducted on 50 ISIAH (inherited stress-induced arterial hypertension) line rats with arterial hypertension: juvenile young (45-day) and sexually mature (100-day) rats, as well as intact animals of the corresponding age. While extracted from the experiment rats of all experimental groups had their arterial pressure measured using a plethysmograph. Electron microscopic examination of the left ventricular myocardium and morphometric study of volumetric and quantitative densities, cross-section area, and form factor of micropinocytotic vesicles were conducted. Results: In sexually mature rats with arterial hypertension, a high level of pressure is maintained. In 45-day-old rats with arterial hypertension in endothelial cells of myocardial blood capillaries there is a hyperactivation of biosynthetic processes (euchromatic nucleus, large-sized mitochondria, ER canals, Golgi complex), which may be a manifestation of reactive processes in response to a non-stable increase in arterial pressure. In the 100-day rats with arterial hypertension, the mosaic of the ultrastructure of the myocardium blood vessels is preserved, but destructively-dystrophic changes become more expressive and involve not only the organelles but also the integrity of the endothelial cell itself. Destructively-dystrophic processes in rat capillaries are accompanied by compensatory and adaptive ones. This is manifested by activation of the transport of substances, both transendothelial and paracellular, and quantitative density of micropinocytotic vesicles increases statistically significantly. Conclusions: In myocardial capillaries of young (45-day) arterial hypertension rats, compensatory and adaptive changes are manifested by activation of biosynthetic processes in endothelial cells following a slight increase in micropinocytotic vesicles quantitative density and signs of destructive-dystrophic processes (minor edema and lysis of endothelial cell cytoplasm). In sexually mature (100-day) arterial hypertension rats in the blood capillaries of the myocardium, the destructive-degenerative changes increase is accompanied by preservation of signs of compensatory processes. Reducing the number of capillaries is offset by an increase in the number of micropinocytotic vesicles.

scholarly journals THE INFLUENCE OF DRUG TREATMENT ON CARDIO-VASCULAR SYSTEM OF THE RATS WITH CONGENITAL HYPOTHYROIDISM

2019 ◽  
Vol 72 (7) ◽  
pp. 1300-1303
Author(s):  
Svetlana Chuhray ◽  
Viktoria Lavrynenko ◽  
Rostyslav Kaminsky ◽  
Olena Ustymenko ◽  
Iryna Dzevulska ◽  
...  
Keyword(s):  
Congenital Hypothyroidism ◽  
Structural Changes ◽  
Vascular System ◽  
Ventricular Myocardium ◽  
Left Ventricular ◽  
The Body ◽  
Left Ventricular Myocardium ◽  
Substitution Therapy ◽  
Blood Capillaries ◽  
Pharmacological Correction

Introduction: In the treatment of hypothyroidism substitution therapy with L-thyroxine is used, it is also advisable to use the metabolites with membrane-stabilizing properties that normalize the metabolism in the body, for example, calcitonin, which significantly reduces the depth of the dystrophic phenomena in the myocardium. The aim was to study the patterns of structural changes in the left ventricular myocardial capillaries of rats with congenital hypothyroidism in combinative drug therapy with L-thyroxine and calcitonin. Materials and methods: 30 white Wistar line rats were used as experimental animals: 10 with treatment and 10 without as well as control – 10 intact Wistar line rats of the same age. Mercazolil was used to inhibit thyroid gland in order to model congenital hypothyroidism. After birth, the rats received L-thyroxine at a dose of 10 μg / kg per os daily, calcitonin at a dose of 1.0 MU / kg per day intramuscularly, then with mother’s milk, later by themselves for 100 days. The arterial pressure was measured in all experimental groups during extraction from the experiment by plethysmograph, their left ventricular myocardium was examined under electron microscope and micropinocytotic vesicles in their cells were studied morphometrically. Results: In rats with congenital hypothyroidism, for which L-thyroxin drug in combination with calcitonin was used as a substitution therapy, after pharmacological correction, in general there is no pronounced heteromorphism of the ultrastructure of the left ventricular myocardial blood capillaries, which was characteristic for animals without pharmacological correction. The analysis showed normalization of the content of free thyroxine in blood plasma and blood pressure of rats with congenital hypothyroidism after complex substitution therapy. Conclusions: In rats with congenital hypothyroidism, which received L-thyroxine and calcitonin at birth, the myocardium capillaries generally remain intact and have morphological and functional characteristics similar to intact animals, which is the theoretical basis for the need for calcitonin to be used in substitution therapy in hypothyroidism.

scholarly journals INFLUENCE OF COMBINED PHARMACOTHERAPY ON CARDIO-VASCULAR SYSTEM OF ARTERIAL HYPERTENSION (IN EXPERIMENT)

2020 ◽  
Vol 73 (8) ◽  
pp. 1712-1716
Author(s):  
Svetlana N. Chuhray ◽  
Viktoria E. Lavrynenko ◽  
Ruzhena M. Matkivska ◽  
Tetiana V. Lachtadyr ◽  
Valentina M. Hamalii ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Arterial Pressure ◽  
Arterial Hypertension ◽  
Structural Changes ◽  
Vascular System ◽  
Ventricular Myocardium ◽  
Left Ventricular ◽  
Morphometric Study ◽  
Left Ventricular Myocardium ◽  
Electron Microscopic

DOI: 10.36740/ BOGOMOLETS NATIONAL MEDICAL UNIVERSITY, KYIV, UKRAINE, TARAS SHEVCHENKO NATIONAL UNIVERSITY OF KYIV, KYIV, UKRAINE 3KYIV CITY CLINICAL EMERGENCY HOSPITAL, KYIV, UKRAINE ABSTRACT The aim: Study of the patterns of structural changes in the left ventricular myocardial capillaries of rats with arterial hypertension with combined pharmacotherapy with Bisoprolol and Thiotriazolinum. Materials and methods: Experiments were conducted on 30 line rats with congenital stress-induced arterial hypertension: 10 animals without treatment and 10 animals with treatment. Pharmacological correction of spontaneous arterial hypertension was performed with 20 mg / kg of Bisoprolol and 50 mg / kg of Thiotriazolinum per os once a day. Pharmacotherapy began at 5 months of age, that is, at a time when compensated heart failure was formed in rats with arterial hypertension. Animals were withdrawn from the experiment 100 days after the start of the correction. Control was provided by intact animals (10 rats) of the corresponding age. While extracted from the experiment rats of all experimental groups had their arterial pressure measured using a plethysmograph, electron microscopic examination of the left ventricular myocardium and morphometric study of volumetric and quantitative densities, cross-section area and form factor of micropinocytotic vesicles were conducted. Results: In rats with arterial hypertension after application of Bisoprolol and Thiotriazolinum, arterial pressure significantly decreases in experimental rats compared to animals without correction. The number of capillaries in the myocardium after pharmacotherapy increases up to control values, which shows their reparation. In most endothelial cells, organelles retain their integrity and presence that are characteristic of intact rats. The well-expressed processes of transcytosis are shown by the statistical similarity of the quantitative density and the size of the micropinocytotic vesicles in the endothelial cells of the myocardium capillaries of compared experimental animals. Conclusions: In rats with arterial hypertension, the combination of Bisoprolol and Thiotriazolinum prevents the decrease in the number of capillaries in the myocardium of the left ventricle, promotes the preservation of the ultrastructure of their endothelial cells and maintains the processes of transedothelial transfer of substances at the level of intact animals.

Deficiency in endothelial nitric oxide synthase impairs myocardial angiogenesis

2002 ◽  
Vol 283 (6) ◽  
pp. H2371-H2378 ◽  
Author(s):  
Xue Zhao ◽  
Xiangru Lu ◽  
Qingping Feng
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Heart Development ◽  
Tube Formation ◽  
Left Ventricular ◽  
Left Ventricular Myocardium ◽  
Wild Type ◽  
Endothelial Nitric Oxide

We recently demonstrated that mice deficient in endothelial nitric oxide (NO) synthase (eNOS) have congenital septal defects and postnatal heart failure. However, the mechanisms by which eNOS affects heart development are not clear. We hypothesized that deficiency in eNOS impairs myocardial angiogenesis. Myocardial capillary densities were measured morphometrically in neonatal mouse hearts. In vitro tube formation on Matrigel was investigated in cardiac endothelial cells. In vivo myocardial angiogenesis was performed by implanting Matrigel in the left ventricular myocardium. Myocardial capillary densities and VEGF mRNA expression were decreased in neonatal eNOS−/− compared with neonatal wild-type mice ( P < 0.01). Furthermore, in vitro tube formation from cardiac endothelial cells and in vivo myocardial angiogenesis were attenuated in eNOS−/− compared with wild-type mice ( P < 0.01). In vitro tube formation was inhibited by N G-nitro-l-arginine methyl ester in wild-type mice and restored by a NO donor, diethylenetriamine-NO, in eNOS−/− mice ( P < 0.05). In conclusion, deficiency in eNOS decreases VEGF expression and impairs myocardial angiogenesis and capillary development. Decreased myocardial angiogenesis may contribute to cardiac abnormalities during heart development in eNOS−/− mice.

scholarly journals CARDIOVASCULAR SYSTEM OF THE MATURE RATS WITH CONGENITAL HYPOTHYROIDISM AND ARTERIAL HYPERTENSION

2020 ◽  
Vol 73 (10) ◽  
pp. 2209-2213
Author(s):  
Svetlana N. Chuhray ◽  
Viktoria E. Lavrynenko ◽  
Rostyslav F. Kaminsky ◽  
Larysa B. Shobat ◽  
Oleksandr I. Kovalchuk ◽  
...  
Keyword(s):  
Arterial Hypertension ◽  
Congenital Hypothyroidism ◽  
Blood Circulation ◽  
Transport Processes ◽  
Electron Microscopic ◽  
Blood Capillaries ◽  
Perivascular Edema ◽  
Enzyme Method ◽  
Hypoxic State ◽  
Left Ventricle Myocardium

The aim: Studying changes in the ultrastructure of blood circulatory capillaries of the myocardium of mature rats with hypothyroidism and arterial hypertension. Materials and methods: Experiments were conducted on (240 days) 10 ISIAH (inherited stress-induced arterial hypertension) line rats with AH (arterial hypertension), 10 Wistar line rats with congenital hypothyroidism and 10 intact animals. Arterial pressure was measured, and the development of hypothyroidism was controlled by the immune enzyme method. The study of the left ventricle myocardium of the rat heart was carried out by electron microscopic and morphometric studies. Results: In in rats with AH the following changes were observed in the blood capillaries of the myocardium: decrease in the number of capillaries; disturbance of blood circulation; the number of organelles of the biosynthetic plan and structures involved in the transendothelial transfer of substances decreased in endothelial cells; lysis and edema of the latter; mucinous perivascular edema, confirmed by the accumulation of fine-fibrillar structures, collagen fibers, cellular detritus. By the same term, in the group with congenital hypothyroidism, dystrophic-destructive changes in the blood capillaries of the myocardium acquired the highest degree, which resulted in a decrease in their number due to destruction. Ultrastructure of the biosynthetic plan organelles and structures of the transendothelial transfer of substances were in decompensated state. Conclusions: The rats (in 240 days) with AH and congenital hypothyroidism express breakdown of compensatory processes in the capillaries of the myocardium. This is manifested by the further dilution of capillaries, the development of hypoxic state in them as well as mucinous edema of interstitium, the decrease of activity of biosynthetic and transport processes.

Hypertrabecular aspect of left ventricular myocardium: A possible complication of congenital hypothyroidism in a preterm infant

2010 ◽  
Vol 23 (7) ◽  
pp. 732-735
Author(s):  
Francesca Paola Fusco ◽  
Paola Sindico ◽  
Maria Pia De Carolis ◽  
Simonetta Costa ◽  
Francesco Cota ◽  
...  
Keyword(s):  
Preterm Infant ◽  
Congenital Hypothyroidism ◽  
Ventricular Myocardium ◽  
Left Ventricular ◽  
Left Ventricular Myocardium

Lasertechniques treatment of vascular malformations

Phlebologie ◽  
2010 ◽  
Vol 39 (03) ◽  
pp. 167-175
Author(s):  
M. Poetke ◽  
P. Urban ◽  
H.-P. Berlien
Keyword(s):  
Endothelial Cells ◽  
Spontaneous Regression ◽  
Vascular System ◽  
Vascular Malformations ◽  
Clinical Importance ◽  
Vascular Structure ◽  
Laser Application ◽  
Vascular Morphogenesis ◽  
Structural Abnormalities

SummaryVascular malformations are structural abnormalities, errors of vascular morphogenesis, which can be localized in all parts of the vascular system. All vascular malformations by definition, are present at birth and grow proportionately with the child; their volume can change. In contrast to the haemangiomas, which only proliferate from the endothelial cells the division in stages is of clinical importance. Vascular malformations are divided from the part of vascular system, which is affected.In principle the techniques of laser application in congenital vascular tumours like haemangiomas and in vascular malformations are similar, but the aim is different. In tumours the aim is to induce regression, in vascular malformations the aim is to destroy the pathologic vascular structure because there is no spontaneous regression. This means that the parameters for treatment of vascular malformations must be more aggressive than for vascular tumours.

scholarly journals Studying dynamic stress effects on the behaviour of THP-1 cells by microfluidic channels

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Semra Zuhal Birol ◽  
Rana Fucucuoglu ◽  
Sertac Cadirci ◽  
Ayca Sayi-Yazgan ◽  
Levent Trabzon
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Vascular System ◽  
Circulatory System ◽  
Disease Process ◽  
Microfluidic Channels ◽  
Shear Stresses ◽  
Stress Effects ◽  
Adhesion Behavior ◽  
Cycling System

AbstractAtherosclerosis is a long-term disease process of the vascular system that is characterized by the formation of atherosclerotic plaques, which are inflammatory regions on medium and large-sized arteries. There are many factors contributing to plaque formation, such as changes in shear stress levels, rupture of endothelial cells, accumulation of lipids, and recruitment of leukocytes. Shear stress is one of the main factors that regulates the homeostasis of the circulatory system; therefore, sudden and chronic changes in shear stress may cause severe pathological conditions. In this study, microfluidic channels with cavitations were designed to mimic the shape of the atherosclerotic blood vessel, where the shear stress and pressure difference depend on design of the microchannels. Changes in the inflammatory-related molecules ICAM-1 and IL-8 were investigated in THP-1 cells in response to applied shear stresses in an continuous cycling system through microfluidic channels with periodic cavitations. ICAM-1 mRNA expression and IL-8 release were analyzed by qRT-PCR and ELISA, respectively. Additionally, the adhesion behavior of sheared THP-1 cells to endothelial cells was examined by fluorescence microscopy. The results showed that 15 Pa shear stress significantly increases expression of ICAM-1 gene and IL-8 release in THP-1 cells, whereas it decreases the adhesion between THP-1 cells and endothelial cells.

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