VITAMIN D DEFICIENCY IN PATIENTS WITH CHRONIC HEART FAILURE AND MORPHO-FUNCTIONAL CONDITION OF PERIPHERAL BLOOD ERYTHROCYTES

The aim: To assess the vitamin D level in blood plasma of patients with chronic heart failure and to identify the effect of its deficiency on the state of peripheral blood erythrocytes during physical exertion. Materials and methods: A total of 25 patients with CHF grade IIA stages II and III functional class were examined. The control group consisted of 25 relatively healthy people. All patients were offered to complete a 6 minutes walking test. The level of 25 (OH) D total in plasma was determined by enzyme immunoanalysis. Morphological studies of erythrocytes were performed on the light-optical (Leica CME) and electron-microscopic level («JEOL-25M-T220»). Results: Patients with chronic heart failure experienced 22.9% decrease in their vitamin D level (17.2±0.04 ng/ml) compared to the control group (38.4±0.05 ng/ml). Correlation analysis showed a direct proportional relationship between vitamin D deficiency and the number of erythrocytes of a modified form (r = 0.58; p <0.05) and erythrocytes with low osmotic resistance (r = 0.87; p <0.05). During the timed physical evaluation patients who experienced chronic heart failure accompanied by vitamin D deficiency developed an increase in the number of their reversibly and irreversibly deformed erythrocytes and a decrease in the cellular osmotic tability. Conclusions: During physical exertion, patients who experienced chronic heart falure accompanied by with vitamin D deficiency experienced morpho-biochemical changes in their red blood cells. These changes indicated structural disturbances in the membranes of their erythrocytes and could potentially have negative consequences for the somatic health of these patients.

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The mechanism of adaptation of the organism of patients with chronic heart failure combined with vitamin D deficiency and the morphofunctional state of peripheral blood erythrocytes

Regulatory Mechanisms in Biosystems ◽

10.15421/021954 ◽

2019 ◽

Vol 10 (3) ◽

pp. 352-357

Author(s):

N. I. Baryla ◽

I. P. Vakaliuk ◽

S. L. Pоpеl’

Keyword(s):

Heart Failure ◽

Vitamin D ◽

Chronic Heart Failure ◽

Red Blood Cells ◽

Vitamin D Deficiency ◽

Peripheral Blood ◽

Blood Cells ◽

Functional Class ◽

Exercise Stress ◽

Control Group

The problem of structural changes in peripheral blood erythrocytes in patients with chronic heart failure in combination with vitamin D deficiency during exercise stress remains insufficiently studied. Vitamin receptors are located on smooth myocytes, endothelial cells, cardiomyocytes and blood cells. It affects the state of the cell membrane, the contractile function of the myocardium, the regulation of blood pressure, cardiac remodeling and reduction of left ventricular hypertrophy. Therefore, it is important to assess the level of vitamin D in blood plasma in individuals with chronic heart failure and to identify the effect of its deficiency on the state of peripheral red blood cells when performing a 6-minute walk test. A total of 75 patients of the main group with chronic heart failure stage II A, I–II functional class with different levels of vitamin D deficiency were examined. The control group included 25 patients with chronic heart failure stage II A, functional class I–II without signs of vitamin D deficiency. The average age of patients was 57.5 ± 7.5 years. All patients were asked to undergo the 6 minutes walking test. The level of total vitamin D in plasma was determined by enzyme immunoassay. Morphological studies of erythrocytes were performed on the light-optical and electron-microscopic level. The obtained results showed that patients of the main group with chronic heart failure had a decrease in vitamin D by 2.2 times compared with the control group. Correlation analysis showed a directly proportional relationship between vitamin D deficiency and the number of red blood cells of a modified form and red blood cells with low osmotic resistance. Dosed exercise stress in patients with chronic heart failure against a background of vitamin D deficiency leads to an increase in the number of reversibly and irreversibly deformed erythrocytes and a decrease in their osmotic stability. This indicates a disorder in the structural integrity of their membrane and can have negative consequences for the somatic health of such patients.

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Vitamin D deficiency and clinical outcome in patients with chronic heart failure: A review

Nutrition Metabolism and Cardiovascular Diseases ◽

10.1016/j.numecd.2017.07.009 ◽

2017 ◽

Vol 27 (10) ◽

pp. 837-849 ◽

Cited By ~ 13

Author(s):

C. D'Amore ◽

F. Marsico ◽

A. Parente ◽

S. Paolillo ◽

F. De Martino ◽

...

Keyword(s):

Heart Failure ◽

Vitamin D ◽

Chronic Heart Failure ◽

Clinical Outcome ◽

Vitamin D Deficiency

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Correlation of Vitamin D Deficiency With Severity of Chronic Heart Failure as Assessed by Functional Class and N-Terminal Pro-Brain Natriuretic Peptide Levels

Cureus ◽

10.7759/cureus.13522 ◽

2021 ◽

Author(s):

Parminder S Otaal ◽

Sudheer Pachipala ◽

Lipi Uppal ◽

Dinakar Bootla

Keyword(s):

Heart Failure ◽

Vitamin D ◽

Chronic Heart Failure ◽

Brain Natriuretic Peptide ◽

Vitamin D Deficiency ◽

Natriuretic Peptide ◽

Functional Class

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Does vitamin D deficiency play a role in the pathogenesis of chronic heart failure? Do supplements improve survival?

Cleveland Clinic Journal of Medicine ◽

10.3949/ccjm.77a.08078 ◽

2010 ◽

Vol 77 (5) ◽

pp. 290-293 ◽

Cited By ~ 2

Author(s):

Victor Hajjar ◽

Jeremiah P. Depta ◽

Maria M. Mountis

Keyword(s):

Heart Failure ◽

Vitamin D ◽

Chronic Heart Failure ◽

Vitamin D Deficiency

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The evaluation of 25(OH)D concentration in blood serum of chronic heart failure patients

Current Issues in Pharmacy and Medical Sciences ◽

10.1515/cipms-2016-0021 ◽

2016 ◽

Vol 29 (3) ◽

pp. 107-110

Author(s):

Tomasz Lewandowski ◽

Andrzej Baginski ◽

Arleta Malecha-Jedraszek ◽

Jerzy Mosiewicz ◽

Helena Donica

Keyword(s):

Heart Failure ◽

Vitamin D ◽

Chronic Heart Failure ◽

Statistical Significance ◽

Venous Blood ◽

Control Group ◽

Total Calcium ◽

Phosphate Homeostasis ◽

Creatinine Concentration ◽

Inorganic Phosphates

Abstract This current study examined patients with severe chronic heart failure (CHF) in order to ascertain the held vitamin D, based on an assessment of calcidiol [25(OH)D] concentration. It also identified and evaluated possible correlations between 25(OH)D level and the concentration of total calcium, inorganic phosphates and creatinine concentration in their serum. Herein, venous blood samples were taken from 36 patients with CHF. Diagnosis was confirmed by echocardiographic, as well as by electrocardiographic examinations. In this work, the control group consisted of 41 randomly selected healthy individuals. The results of our study showed that CHF patients had significantly lower concentration of 25(OH)D, as well as total calcium and inorganic phosphates. Moreover, mean creatinine concentration was higher, in comparison to the control group, but did not exhibit statistical significance. As calcium-phosphate homeostasis is regulated by numerous factors, including PTH, neurohormonal factors and calcitriol (1,25(OH)2D), it is possible that vitamin D deficiency may play a significant role in the pathomechanism of CHF, and a lowered 25(OH)D level may be related to progression of the disease.

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