scholarly journals The mechanism of adaptation of the organism of patients with chronic heart failure combined with vitamin D deficiency and the morphofunctional state of peripheral blood erythrocytes

2019 ◽  
Vol 10 (3) ◽  
pp. 352-357
Author(s):  
N. I. Baryla ◽  
I. P. Vakaliuk ◽  
S. L. Pоpеl’
Keyword(s):  
Heart Failure ◽  
Vitamin D ◽  
Chronic Heart Failure ◽  
Red Blood Cells ◽  
Vitamin D Deficiency ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Functional Class ◽  
Exercise Stress ◽  
Control Group

The problem of structural changes in peripheral blood erythrocytes in patients with chronic heart failure in combination with vitamin D deficiency during exercise stress remains insufficiently studied. Vitamin receptors are located on smooth myocytes, endothelial cells, cardiomyocytes and blood cells. It affects the state of the cell membrane, the contractile function of the myocardium, the regulation of blood pressure, cardiac remodeling and reduction of left ventricular hypertrophy. Therefore, it is important to assess the level of vitamin D in blood plasma in individuals with chronic heart failure and to identify the effect of its deficiency on the state of peripheral red blood cells when performing a 6-minute walk test. A total of 75 patients of the main group with chronic heart failure stage II A, I–II functional class with different levels of vitamin D deficiency were examined. The control group included 25 patients with chronic heart failure stage II A, functional class I–II without signs of vitamin D deficiency. The average age of patients was 57.5 ± 7.5 years. All patients were asked to undergo the 6 minutes walking test. The level of total vitamin D in plasma was determined by enzyme immunoassay. Morphological studies of erythrocytes were performed on the light-optical and electron-microscopic level. The obtained results showed that patients of the main group with chronic heart failure had a decrease in vitamin D by 2.2 times compared with the control group. Correlation analysis showed a directly proportional relationship between vitamin D deficiency and the number of red blood cells of a modified form and red blood cells with low osmotic resistance. Dosed exercise stress in patients with chronic heart failure against a background of vitamin D deficiency leads to an increase in the number of reversibly and irreversibly deformed erythrocytes and a decrease in their osmotic stability. This indicates a disorder in the structural integrity of their membrane and can have negative consequences for the somatic health of such patients.

scholarly journals VITAMIN D DEFICIENCY IN PATIENTS WITH CHRONIC HEART FAILURE AND MORPHO-FUNCTIONAL CONDITION OF PERIPHERAL BLOOD ERYTHROCYTES

2020 ◽  
Vol 73 (8) ◽  
pp. 1610-1614
Author(s):  
Nadiia I. Baryla ◽  
Igor P. Vаkаlyuk ◽  
Sergii L. Popel’
Keyword(s):  
Heart Failure ◽  
Vitamin D ◽  
Chronic Heart Failure ◽  
Vitamin D Deficiency ◽  
Peripheral Blood ◽  
Physical Exertion ◽  
Electron Microscopic Level ◽  
Control Group ◽  
Negative Consequences ◽  
Morphological Studies

The aim: To assess the vitamin D level in blood plasma of patients with chronic heart failure and to identify the effect of its deficiency on the state of peripheral blood erythrocytes during physical exertion. Materials and methods: A total of 25 patients with CHF grade IIA stages II and III functional class were examined. The control group consisted of 25 relatively healthy people. All patients were offered to complete a 6 minutes walking test. The level of 25 (OH) D total in plasma was determined by enzyme immunoanalysis. Morphological studies of erythrocytes were performed on the light-optical (Leica CME) and electron-microscopic level («JEOL-25M-T220»). Results: Patients with chronic heart failure experienced 22.9% decrease in their vitamin D level (17.2±0.04 ng/ml) compared to the control group (38.4±0.05 ng/ml). Correlation analysis showed a direct proportional relationship between vitamin D deficiency and the number of erythrocytes of a modified form (r = 0.58; p <0.05) and erythrocytes with low osmotic resistance (r = 0.87; p <0.05). During the timed physical evaluation patients who experienced chronic heart failure accompanied by vitamin D deficiency developed an increase in the number of their reversibly and irreversibly deformed erythrocytes and a decrease in the cellular osmotic tability. Conclusions: During physical exertion, patients who experienced chronic heart falure accompanied by with vitamin D deficiency experienced morpho-biochemical changes in their red blood cells. These changes indicated structural disturbances in the membranes of their erythrocytes and could potentially have negative consequences for the somatic health of these patients.

scholarly journals Reduced Half-Life of Red Blood Cells Causes Anemia in the Model of Hypertensive Chronic Heart Failure

2016 ◽  
Vol 22 (9) ◽  
pp. S206
Author(s):  
Eri Manabe ◽  
Naoko Sasaki ◽  
Yoshiya Ohno ◽  
Toshiyuki Tanaka ◽  
Satoyasu Ito ◽  
...  
Keyword(s):  
Heart Failure ◽  
Chronic Heart Failure ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Half Life

Vitamin D deficiency and clinical outcome in patients with chronic heart failure: A review

2017 ◽  
Vol 27 (10) ◽  
pp. 837-849 ◽  
Author(s):  
C. D'Amore ◽  
F. Marsico ◽  
A. Parente ◽  
S. Paolillo ◽  
F. De Martino ◽  
...  
Keyword(s):  
Heart Failure ◽  
Vitamin D ◽  
Chronic Heart Failure ◽  
Clinical Outcome ◽  
Vitamin D Deficiency

scholarly journals Prognostic role of metabolic risk factors (hyperhomocysteinemia) in the development of chronic heart failure

2015 ◽  
Vol 18 (1) ◽  
pp. 20
Author(s):  
Ye. N. Berezikova ◽  
M. G. Pustovetova ◽  
S. N. Shilov ◽  
A. V. Yefremov ◽  
A. T. Teplyakov ◽  
...  
Keyword(s):  
Heart Failure ◽  
Chronic Heart Failure ◽  
Blood Serum ◽  
Homocysteine Level ◽  
Functional Class ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Relationship Of ◽  
The Relationship

The aim of the study was to assess the relationship of homocysteine levels in the blood serum with the severity and nature of chronic heart failure (CHF) in patients with coronary heart disease. 94 patients with CHF were examined. The control group included 32 patients without cardiovascular disorders. At baseline and after 12 months of observation the homocysteine levels in the blood serum were determined by enzyme-linked immunosorbent assay. Correlative relationship of hyperhomocysteinemia with ischemic myocardial remodeling and with reduced inotropic function in CHF patients was observed. The homocysteine level in the blood serum of CHF patients significantly exceeded that of the control group and moderately increased with the progression of the disease severity of functional class. In the group with an unfavorable course of CHF the baseline homocysteine level proved to have the greatest value as compared to that in the group with a favorable course. In patients with a favorable course of CHF the homocysteinemia level tended to decrease towards the end of prospective study, whereas in patients with an unfavorable course, on the contrary, hyperhomocysteinemia persisted (p<0.01). In case the hyperhomocysteinemia baseline exceeds 18.5 mkmol/L (ROC-AreaSE = 0.860.04, sensitivity 71 %, specificity 90%), the severity and characteristics of the ischemic CHF could be most likely predicted. Thus, hyperhomocysteinemia is related with the severity and nature of CHF. Determining the level of homocysteine in the blood serum can be recommended for early prediction of the severity and nature of CHF.

Structural-functional state of erythrocytes in children with chronic kidney disease

2019 ◽  
Vol 1 (7) ◽  
pp. 105-108
Author(s):  
A. V. Sabirova ◽  
D. K. Volosnikov ◽  
O. V. Matyash
Keyword(s):  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Red Blood Cells ◽  
Functional State ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Early Stage ◽  
Control Group ◽  
Healthy Children ◽  
Range Analysis

During Chronic Kidney Disease (CKD) development and progression emphasis is placed on structural-functional state of red blood cells. The aim of this paper is to examine red blood values and red blood cells morphological type in peripheral blood among children with CKD. 75 children with CKD aged 5-16 years were examined. The control group consisted of 25 healthy children of the same age range. Analysis of the morphometric parameters of red peripheral blood cells - mean volume (MCV) and erythrocyte diameter, mean content (MCH) and mean hemoglobin concentration in the erythrocyte (MCHC), were performed using a Gobas Micros (Roche) hematology counter. Smears were marked according to Romanovsky-Giemsa. Morphometric studies of red blood cells were carried out using the Morphology 5.2 program and red blood cell confocal microscopy on a LSM-710 Confocal Microscope, manufactured by Carl Zeiss. The tendency to poikilocytosis in CKD was confirmed. We identified that among children with CKD the distribution of red blood cells in diameter is disturbed, the content of microcytes increases, poikilocytosis is observed with an increase in the content of irreversibly transformed elements. The revealed features may serve as an additional criterion for the diagnosis of chronic kidney insufficiency at the early stage.

scholarly journals Study of blood levels of Lactate, Vitamin D and Nucleated Red Blood Cells for early prediction of Hypoxic–Ischemic Encephalopathy: A case-control study

2021 ◽  
Author(s):  
Ahmed Hosni Saad ◽  
Ahmed Thabet Mahmoud ◽  
Mohamed Abdelrehem Soliman ◽  
Alyaa Ahdy Abdelaziz
Keyword(s):  
Vitamin D ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Venous Blood ◽  
White Blood Cells ◽  
Control Group ◽  
Blood Levels ◽  
P Value ◽  
Nucleated Red Blood Cells ◽  
Control Study

Abstract Objective and background: Perinatal asphyxia is a serious medical condition in which there is lack of oxygen or blood flow during, or even after the labor process. So, this study aimed to study serum blood levels of Lactate, vitamin D and count of nucleated Red Blood Cells (nRBCs) per 100 White Blood Cells (WBCs) as early biological predictors of Hypoxic–Ischemic Encephalopathy (HIE) after birth Asphyxia.Methods: A retrospective case-control study was conducted on 20 neonates with HIE comprising the cases (APGAR ≤ 5) and another 20 neonates representing the control group with their age, sex, and weight matched, admitted to NICU from March 2020 to the end of September 2020 at Menoufia University Hospital and El-Bagour Central Hospital, Egypt. Venous blood samples for lactate, vitamin D and nucleated RBCs were drawn during 1st 6h of birth and sent for analysis. Sarnat score was used to assess the severity of HIE.Results: Venous blood levels of lactate and nucleated Red Blood Cells per 100 White Blood Cells (nRBC/100 WBC) counts were significantly higher in HIE neonates than in the control group with a highly significant difference (P-value < 0.001). While serum Vitamin D was significantly lower in the HIE group than in the control (P-value < 0.001).Conclusion: Serum lactate level, vitamin D level and nRBCs/100 WBCs might be used as biomarkers in the early prediction of HIE.

Role of soluble Fas ligand in myocardial remodeling, severity and outcomes of chronic heart failure

2016 ◽  
Vol 88 (9) ◽  
pp. 10-16 ◽  
Author(s):  
A T Teplyakov ◽  
E N Berezikova ◽  
S N Shilov ◽  
E V Grakova ◽  
Yu Yu Torim ◽  
...  
Keyword(s):  
Heart Failure ◽  
Chronic Heart Failure ◽  
Fas Ligand ◽  
Functional Class ◽  
Control Group ◽  
Myocardial Remodeling ◽  
Soluble Fas ◽  
Soluble Fas Ligand

Aim. To reveal the specific features of Fas ligand-mediated ischemic myocardial remodeling and those of chronic heart failure (CHF) development during a 12-month prospective follow-up. Subjects and methods. A total of 94 patients with ischemic CHF were examined and divided into 3 groups according to NYHA Functional Class (FC): 1) FC II CHF in 35 patients; 2) FC III CHF in 31; 3) FC IV CHF in 28. According to the results of the 12-month follow-up, the patients were randomized into 2 groups: A) 49 patients with a favorable course of cardiovascular disease and B) 45 patients with its poor course. Serum soluble Fas ligand (sFas-L) levels were measured by enzyme immunoassay. Results. In the patients with CHF, the baseline sFas-L levels substantially exceeded that in the control group by 3—6 times (p

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