Leaf Micromorphology and Pollen Grain Characteristics of Nicotiana glauca. R.C. Graham and Nicotiana tabacum. L.(Solanaceae, Nicotianoideae)

2021 ◽  
pp. 25-33
Author(s):  
Farag M. El-Mokasabi ◽  
Abdelbaset M. Asker ◽  
Raja M. El-Mabrouk
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Nicotiana Tabacum ◽  
Light Microscope ◽  
Pollen Grain ◽  
Nicotiana Glauca ◽  
Leaf Venation ◽  
Leaf Micromorphology ◽  
Nicotiana Tabacum L ◽  
Scanning Electron

The micromorphological variation of the leaf and pollen grain characteristics in two species of Nicotiana was examined using a light microscope (LM) and scanning electron microscopy (SEM). These species are Nicotiana glauca R.C. Graham (wild species) and Nicotiana tabacum L. (cultivated species). The leaves are simple. The other leaf micromorphological features presented variation. The leaf architectural characteristics were also observed. Leaf venation is pinnate brochidodromous. The epidermal cell shape, on the abaxial leaf surface, is polygonal to irregular in Nicotiana glauca but it is so adaxially in all the two species the cells are irregular on both the epidermal layers. The anticlinal walls are either straight or sinuous. The leaves are amphistomatic. The stomata are commonly anomocytic to anisocytic. Foliar trichomes are glandular and non-glandular. Some of the foliar micromorphological characters as viewed under SEM were beneficial in species identification. The pollen shape is prolate with aperture tricolpate without opercula, the colpi shallow sunken with their ends acute. The exine sculpture is reticulate with granulate or granulate-microperforated to fine granulate and irregularly reticulate to perforate. Keywords: Leaf micromorphology- Pollen grains - Light microscope (LM); Scanning electron microscopy (SEM)- Nicotiana Solanaceae -Nicotianoideae.

Fine Structure of Pollen Grain Ektexine of Maize, Teosinte and Tripsacum

1972 ◽  
Vol 30 ◽  
pp. 226-227
Author(s):  
Umesh C. Banerjee ◽  
Elso S. Barghoorn
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Zea Mays ◽  
Fine Structure ◽  
Light Microscope ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Wild Relatives ◽  
Distilled Water ◽  
Scanning Electron

Using light microscope (LM) it was found very difficult to distinguish the ektexine (outer sculptured layer of exine) pattern of maize (Zea mays L.) pollen grains from that of its wild relatives teosinte (Euchlaena mexicana Schrad.) and tripsacum (Tripsacum spp.). At the magnifications obtained by LM, the pollen grain ektexine is faintly granular or netted. By the use of electron microscopy, however, it is possible to characterize their pollen ektexine patterns.For scanning electron microscopy (SEM) acetolysed pollen grains were used. After acetolysis the pollen samples were washed several times in glass-distilled water to remove traces of acids. Each sample was dispersed in a drop of distilled water placed on specimen holders.

scholarly journals Scanning Electron Microscopy Observations of Loa loa (Nematoda)

2020 ◽  
Vol 11 (2) ◽  
pp. 486-492
Author(s):  
Jens Anibal Juul ◽  
Vegard Asgeir Forsaa ◽  
Tor Paaske Utheim ◽  
Endre Willassen
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Case Report ◽  
Scanning Electron Microscope ◽  
Light Microscope ◽  
Loa Loa ◽  
Scanning Electron

We present a case report of periocular Loa loa. The key feature of L. loa distinguishing it from other human filarial parasites are cuticular bosses, which are presented in images from a light microscope and a scanning electron microscope. The cuticular bosses could be divided into three subtypes not previously described.

Mucilaginous film production by plant cells immobilised in a polyurethane or nylon matrix

1985 ◽  
Vol 63 (12) ◽  
pp. 2357-2363 ◽  
Author(s):  
M. J. C. Rhodes ◽  
R. J. Robins ◽  
R. J. Turner ◽  
J. I. Smith
Keyword(s):  
Thin Film ◽  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscope ◽  
Plant Cells ◽  
The Matrix ◽  
Nylon Fibre ◽  
Scanning Electron

The surface features of plant cells immobilised in a matrix of either reticulated polyurethane foam or nylon fibre have been examined with the scanning electron microscope. It has been found that both cells and matrix are enveloped in a thin film, the appearance of which is very dependent on the method by which material is prepared for scanning electron microscopy. The structure is severely damaged by fixation and dehydration. Only in specimens examined in the frozen hydrated state is a structure seen compatible with that observed with the light microscope. From the way the appearance of the film is affected by different methods of preparation for the scanning electron microscope, it is suggested that the film is a hydrated mucilage. The importance of this film for the retention of cells within the matrix is discussed.

scholarly journals Pollen grain surface in Vaccinium myrtillus as seen in scanning electron microscopy

2014 ◽  
Vol 50 (4) ◽  
pp. 583-584
Author(s):  
Józef Kocoń ◽  
Kazimierz Pliszka ◽  
Stanisław Muszyński
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Vaccinium Myrtillus ◽  
Grain Surface ◽  
Scanning Electron

Pollen grain surface of <em>Vaccinium myrtillus</em> L. was analyzed by scanning electron microscopy. Pollen grains remain in tetrahedral tetrads. Grain surface is verrucose, consisting of thick, irregularly shaped muri, surrounding small, round or oval lumina. The surface of the muri is fissured, and minute papillae can also be noted.

Comparison of Larval Stored Product Beetle Mandibles by Light Microscope and Scanning Electron Microscope

1981 ◽  
Vol 64 (1) ◽  
pp. 199-224
Author(s):  
John E Kvenberg
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Light Microscope ◽  
Morphological Characteristics ◽  
Scanning Electron ◽  
Conventional Light Microscopy

Abstract Larval stored product beetle mandibles were studied by comparing images made by scanning electron microscopy with those made by conventional light microscopy. Discussion of morphological characteristics is based on illustrations of 25 species

Stipa ×fallax (Poaceae: Pooideae: Stipeae), a new natural hybrid from Tajikistan, and a new combination in Stipa drobovii

Phytotaxa ◽  
2017 ◽  
Vol 303 (2) ◽  
pp. 141 ◽  
Author(s):  
MARCIN NOBIS ◽  
ARKADIUSZ NOWAK ◽  
AGNIESZKA NOBIS ◽  
SYLWIA NOWAK ◽  
JUSTYNA ŻABICKA ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Central Asia ◽  
Field Observation ◽  
Parental Species ◽  
Pollen Grain ◽  
Numerical Analyses ◽  
New Combination ◽  
Natural Hybrid ◽  
Scanning Electron

Stipa ×fallax nothosp. nov. (Poaceae), from western Pamir Alai Mts (Tajikistan), is described and illustrated. Field observation, numerical analyses of morphology, and pollen grain viability data show that it originated from hybridization between S. drobovii and S. macroglossa subsp. macroglossa, species representing sections Smirnovia and Stipa, respectively. Stipa ×fallax is morphologically close to S. ×alaica and S. ×hissarica, but is distinguished by its shortly pilose lower part of the awn and densely pubescent leaves. Characters distinguishing S. ×fallax from its parental species as well as similar hybrid taxa in section Smirnovia that also grow in Central Asia are presented. The micromorphology of lemmas and leaves of S. ×fallax and its parental species was examined by scanning electron microscopy. We also propose the new combination Stipa drobovii var. iskanderkulica (Tzvelev) M.Nobis & A.Nowak.

scholarly journals Pollen morphology of Prototulbaghia Vosa: A comparative palynological study of the Southern African Alliaceae.

2013 ◽  
Vol 32 (1) ◽  
Author(s):  
Melissa Andriessen ◽  
Madeleen Struwig ◽  
Stefan J. Siebert
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grain ◽  
Evolutionary Significance ◽  
Monotypic Genus ◽  
Taxonomic Characteristic ◽  
Unknown Event ◽  
Palynological Study ◽  
Scanning Electron

The  Southern  African  Alliaceae  Borkh.  is  represented  by  four genera (Allium L., Nothoscordum Kunth, Tulbaghia L. and Prototulbaghia Vosa) and 28 species. The  pollen  morphology  of  the  endangered  monotypic  genus Prototulbaghia has  not  been described  before.  A  comparative  study  of  the  pollen  morphology  of Prototulbaghia  siebertii Vosa, Nothoscordum  borbonicum Kunth, Tulbaghia  simmleri P.Beauv.  and T.  violaceae Harv.  is presented in this article. Scanning electron microscopy, as well as light microscopy, were used to examine the pollen. The pollen morphology of the species can be described as perprolate and monosulcate, and the surface sculpture as reticulate and heterobrochate. However, the pollen of Prototulbaghia siebertii displays a unique characteristic as the grains are folded in their breadth with the tips touching, hence causing the grain to display a triangular and disulcate appearance. It might be possible to ascribe this fold to the process of harmomegathy or a still unknown event that occurs during the development of the pollen grain. This phenomenon should be further investigated to determine the cause of folding and whether it is a unique taxonomic  characteristic  of  this  genus,  and  if  it  could  be  of  evolutionary  significance  for the Alliaceae.

Urediniospore morphology of Puccinia species attacking Cardueae

1983 ◽  
Vol 61 (7) ◽  
pp. 2047-2051 ◽  
Author(s):  
J. A. Traquair ◽  
E. G. Kokko
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscope ◽  
Morphological Features ◽  
Spore Ornamentation ◽  
Evolutionary Lineages ◽  
Scanning Electron

Scanning electron microscopy of the urediniospores of three Puccinia species further resolved their spore ornamentation and supported observations made with the light microscope. In particular, the smooth hilum surface of P. jaceae Otth. urediniospores was clearly observed in contrast to the minutely verrucose and distinctly verrucose hilum of P. centaureae D. C. and P. carthami Cda., respectively. These observations in combination with other morphological features of urediniospores were useful in delimiting the three species and in recognizing two urediniospore forms characteristic of separate evolutionary lineages.

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